Adipose/Fat - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Adipocytes Collagenase Type 1: 40 u/ml
 Krebs-Ringer bicarbonate Yang, Y., Baldwin, R.: Preparation and Metabolism of Isolated Cells from Bovine Adipose Tissue, J Dairy Sci 56, 350, 1973
Canine Canine Renal adipose derived cells Collagenase Type 1: 0.3%
 DMEM Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney.,Lipids Health Dis Vol. 10,, , 171, 2011
Canine Canine, 20-25 kg Adipose stem cells Collagenase: see reference
 Media-199 Fischer, L., McIlhenny, S., Tulenko, T., Golesorkhi, N., Zhang, P., Larson, R., Lombardi, J., Shapiro, I. and DiMuzio, P.: Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force., J Surg Res 152, 157, 2009
Canine Canine White fat Collagenase: 0.05%
 Kreb's Ringer bicarbonate buffer DiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species, Am J Physiol 221, 850, 1971
Equine Equine Adipose derived stem cells Collagenase Type 1: 0.1%
 PBS Vidal, M., Robinson, S., Lopez, M., Paulsen, D., Borkhsenious, O., Johnson, J., Moore, R. and Gimble, J.: Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow.,Vet Surg Vol. 37,, , 713, 2008
Equine Equine, 1-5 year Adipose derived stem cells Collagenase Type 1: 0.1%
 PBS Vidal, M., Kilroy, G., Lopez, M., Johnson, J., Moore, R. and Gimble, J.: Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells.,Vet Surg Vol. 36,, , 613, 2007
Fish Fish, Atlantic salmon Preadipocytes Collagenase Type 1: 0.1%
 HBSS Todorcevic, M., Vegusdal, A., Gjoen, T., Sundvold, H., Torstensen, B., Kjaer, M. and Ruyter, B.: Changes in Fatty Acids Metabolism During Differentiation of Atlantic Salmon Preadipocytes; Effects of n-3 and n-9 Fatty Acids., Biochim Biophys Acta 1781, 326, 2008
Gerbil Gerbil of unknown age
(also rat, hamster, rabbit, lamb, guinea-pig)		 Brown fat Collagenase Type 1: 0.10%
 Bicarbonate buffer Nedergaard, J. and Lindberg, O.: The Brown Fat Cell, Int Rev Cytol 74, 187, 1982
Guinea-Pig Guinea-pig, adult
(also rat, hamster, gerbil, rabbit, lamb)		 Brown fat Collagenase Type 1: 0.10%
 Bicarbonate buffer Nedergaard, J. and Lindberg, O.: The Brown Fat Cell, Int Rev Cytol 74, 187, 1982
Hamster Hamster, adult
(also rat, gerbil, rabbit, lamb, guinea-pig)		 Brown fat Collagenase Type 1: 0.10%
 Bicarbonate buffer Nedergaard, J. and Lindberg, O.: The Brown Fat Cell, Int Rev Cytol 74, 187, 1982
Hamster Hamster, 5 week-12 month White fat Collagenase: 0.05%
 Kreb's Ringer bicarbonate buffer DiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species, Am J Physiol 221, 850, 1971
Human Human, male 40-60 years Adipose derived stem cells Collagenase: 0.25%
Deoxyribonuclease I: 0.002%
 PBS Blasi, A., Martino, C., Balducci, L., Saldarelli, M., Soleti, A., Navone, S., Canzi, L., Cristini, S., Invernici, G., Parati, E. and Alessandri, G.: Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential,Vasc Cell Vol. 3,, , 5, 2011
Human Human Renal adipose derived cells Collagenase Type 1: 0.3%
 DMEM Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney.,Lipids Health Dis Vol. 10,, , 171, 2011
Human Human Adipocytes Collagenase Type 1: 0.1%
 KRB Peters, R., Wolf, M., van den Broek, M., Nuvolone, M., Dannenmann, S., Stieger, B., Rapold, R., Konrad, D., Rubin, A., Bertino, J., Aguzzi, A., Heikenwalder, M. and Knuth, A.: Efficient Generation of Multipotent Mesenchymal Stem Cells from Umbilical Cord Blood in Stroma-Free Liquid Culture., PLoS ONE 5, e15689, 2010
Human Human Adipose derived stromal cells Collagenase Type 1: see reference
 DMEM Traktuev, D., Merfeld-Clauss, S., Li, J., Kolonin, M., Arap, W., Pasqualini, R., Johnstone, B., and March, K.: A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks, Circ Res 102, 77, 2008
Human Human Adipocytes Collagenase Type 1: 0.2%
 HBSS Bujalska Iwona J, Durrani Omar M, Abbott Joseph, Onyimba Claire U, Khosla Pamela, Moosavi Areeb H, Reuser Tristan T Q, Stewart Paul M, Tomlinson Jeremy W, Walker Elizabeth A, Rauz Saaeha: Characterisation of 11beta-hydroxysteroid dehydrogenase 1 in human orbital adipose tissue: a comparison with subcutaneous and omental fat, J Endocrinol 192, 279-88, 2007
Human Human Adipose derived stromal cells Collagenase Type 1: 0.15%
 DMEM Schaffler A, Buchler C: Concise review: adipose tissue-derived stromal cells--basic and clinical implications for novel cell-based therapies, Stem Cells 25, 818-27, 2007
Human Human Mesenchymal stem Collagenase Type 1: 0.1%
 HBSS Jeon Eun Su, Song Hae Young, Kim Mi Ra, Moon Hyun Jung, Bae Yong Chan, Jung Jin Sup, Kim Jae Ho: Sphingosylphosphorylcholine induces proliferation of human adipose tissue-derived mesenchymal stem cells via activation of JNK, J Lipid Res 47, 653-64, 2006
Human Human Adipocytes, stromal vascular Collagenase: 0.2%
 HBSS Boquest Andrew C, Shahdadfar Aboulghassem, Fronsdal Katrine, Sigurjonsson Olafur, Tunheim Siv H, Collas Philippe, Brinchmann Jan E: Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture, Mol Biol Cell 16, 1131-41, 2005
Human Human Multipotent adipose derived stem Collagenase: 0.2%
 DMEM Rodriguez Anne-Marie, Pisani Didier, Dechesne Claude A, Turc-Carel Claude, Kurzenne Jean-Yves, Wdziekonski Brigitte, Villageois Albert, Bagnis Claude, Breittmayer Jean-Philippe, Groux Herve, Ailhaud Gerard, Dani Christian: Transplantation of a multipotent cell population from human adipose tissue induces dystrophin expression in the immunocompetent mdx mouse, J Exp Med 201, 1397-405, 2005
Human Human Adult stem cells Collagenase Type 1: 0.1%
 PBS Devireddy, R., Thirumala, S. and Gimble, J.: Cellular Response of Adipose Derived Passage-4 Adult Stem Cells to Freezing Stress., J Biomech Eng 127, 1081, 2005
Human Human, adult, obese Adipocytes Collagenase Type 2: 0.1%
 DMEM/F12 Seboek, D., Linscheid, P., Zulewski, H., Langer, I., Christ-Crain, M., Keller, U., and Muller, B.: Somatostatin Is Expressed and Secreted by Human Adipose Tissue Upon Infection and Inflammation, J Clin Endocrinol Metab 89(10), 4833, 2004
Human Human, adult, female Preadipocytes Collagenase Type 1: 0.2%
 DMEM/F-12 Quickler, M., Sinha, B., Tomlinson, J., Bujalska, I., Stewart, P., Arlt, W.,: Androgen generation in adipose tissue in women with simple obesity--a site-specific role for 17beta-hydroxysteroid dehydrogenase type 5, J Endocrinol 183, 331, 2004
Human Human, adult Adipocytes Collagenase Type 1: 0.13%
 see reference Fain JN, Madan AK, Hiler ML, Cheema P, and Bahouth SW.: Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humans, Endocrinology 145, 2273, 2004
Human Human Stromal vascular, adipocytes Collagenase: 0.2%
 DMEM/F12 Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis: Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives, Circulation 109, 656-63, 2004
Human Human Stromal vascular, adipocytes, stem Collagenase: 300 u/ml
 PBS Miranville A, Heeschen C, Sengenes C, Curat CA, Busse R, Bouloumie A: Improvement of postnatal neovascularization by human adipose tissue-derived stem cells, Circulation 110, 349-55, 2004
Human Human, adult, female, non-obese Adipocytes Collagenase Type 2: 0.05%
 DMEM Gesta, S., Lolmede, K., Daviaud, D., Berlan, M., Bouloumie, A., Lafontan, M., Valet, P., and Saulnier-Blache, J.: Culture of human adipose tissue explants leads to profound alteration of adipocyte gene expression, Horm Metab Res 35, 158, 2003
Human Human Adipocytes Collagenase Type 1: see reference
 Saline Patwardhan, R., Tubbs, R., Leonard, R., Kelly, D., Killingsworth, C., Rollins, D., Smith, W., Ideker, R., and Oakes, W.: Discernment of Adipose versus Nervous Tissue: A Novel Adjunct Solution in Lipomyelomeningocele Surgery, Ped Neurosurg 36, 314, 2002
Human Human, adult, male and female Adipocytes Collagenase Type 1: 0.2%
 DMEM/F12 McTernan, P., Anderson, L., Anwar, A., Eggo, M., Crocker, J., Barnett, A., Stewart, P., and Kumar, S.: Glucocorticoid Regulation of P450 Aromatase Activity in Human Adipose Tissue: Gender and Site Differences, J Clin Endocrinol Metab 87, 1327, 2002
Human Human Processed lipoaspirate cells Collagenase Type 1: 0.075%
 PBS Zuk Patricia A, Zhu Min, Ashjian Peter, De Ugarte Daniel A, Huang Jerry I, Mizuno Hiroshi, Alfonso Zeni C, Fraser JohnK, Benhaim Prosper, Hedrick Marc H: Human adipose tissue is a source of multipotent stem cells, Mol Biol Cell 13, 4279-95, 2002
Human Human Adipocytes Collagenase Type 1: 0.2%
 HBSS McTernan PG, Anwar A, Eggo MC, Barnett AH, Stewart PM, Kumar S: Gender differences in the regulation of P450 aromatase expression and activity in human adipose tissue, Int J Obes Relat Metab Disord 24, 875-81, 2000
Human Human, adult, male and female Adipocytes Collagenase Type 1: 0.1%
 DMEM/Ham's F-12 Gottschling-Zelle, H., Birgel, M., Scriba, D., Blum, W., and Hauner, H.: Depot-specific Release of Leptin from Subcutaneous and Omental Adipocytes in Suspension Culture: Effect of Tumor Necrosis Factor-alpha and Transforming Growth Factor-beta1, Eur J Endocrinol 141 (4), 436, 1999
Human Human, adult, male and female Adipocytes Collagenase Type 1: 0.1%
 DMEM/F12 Zhang, H., Kumar, S., Barnett, A., and Eggo, M.: Intrinsic Site-Specific Differences in the Expression of Leptin in Human Adipocytes and Its Autocrine Effects on Glucose Uptake, J Clin Endocrinol Metab 84, 2550, 1999
Human Human, non-diabetic, male Adipocytes Collagenase Type 1: 0.05%
 Kreb's Ringer bicarbonate buffer Anderson, O., Gliemann, J., and Gammeltoft: Receptor Binding and Biological Effect of Insulin in Human Adipocytes, Diabetologia 13, 589, 1977
Mouse Mouse Stem and progenitor Collagenase Type 2: 0.2%
 HBSS Han, J., Koh, Y., Moon, H., Ryoo, H., Cho, C., Kim, I. and Koh, G.: Adipose Tissue is an Extramedullary Reservoir for Functional Hematopoietic Stem and Progenitor Cells., Blood 115, 957, 2010
Mouse Mouse, 3 week Adipocytes Collagenase Type 1: 0.2%
 HBSS De Matteis, R., Zingaretti, M., Murano, I., Vitali, A., Frontini, A., Giannulis, I., Barbatelli, G., Marcucci, F., Bordicchia, M., Sarzani, R., Raviola, E. and Cinti, S.: In Vivo Physiological Transdifferentiation of Adult Adipose Cells., Stem Cells 27, 2761, 2009
Mouse Mouse White adipocytes Collagenase Type 2: 0.1%
 DMEM Wong, K., Szeto, F, Zhang, W., Ye, H., Kong, J., Zhang, Z., Sun, X. and Li, Y: Involvement of the Vitamin D Receptor in Energy Metabolism: Regulation of Uncoupling Proteins., Am J Physiol/Endo 296, 820, 2009
Mouse Mouse, C57Bl/6J Adipocytes Collagenase Type 1: 0.15%
 DMEM/F12 Aoyagi T, Shimba S, Tezuka M: Characteristics of Circadian Gene Expressions in Mice White Adipose Tissue and 3T3-L1 Adipocytes, J Health Sci 51, 21, 2005
Mouse Mouse, 3-6 day and 2-3 month Adipose-derived stromal cells Collagenase Type 2: 0.075%
 PBS Cowan Catherine M, Shi Yun-Ying, Aalami Oliver O, Chou Yu-Fen, Mari Carina, Thomas Romy, Quarto Natalina, Contag Christopher H, Wu Benjamin, Longaker Michael T: Adipose-derived adult stromal cells heal critical-size mouse calvarial defects, Nat Biotechnol 22, 560-7, 2004
Mouse Mouse Stromal vascular, adipocytes Collagenase: 0.2%
 DMEM/F12 Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis: Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives, Circulation 109, 656-63, 2004
Mouse Mouse, C57BL/6J and FVB, male, 8-9 week Adipocytes Collagenase: see reference
 DMEM Ruan, H., Zarnowski, MJ., Cushman, S., and Lodish, H.: Standared Isolation of Primary Adipose Cells from Mouse Epididymal Fat Pads Induces Inflammatory Mediators and Down-regulates Adipocyte Genes, J Biol Chem 278, 47585, 2003
Mouse Mouse, both sexes Adipocytes Collagenase: 0.05%
 Krebs-Ringer Phosphate HEPES (KRPH) Nadler, S., Stoehr, J., Rabaglia, M., Schueler, K., Birnbaum, M., and Attie, A.: Normal Akt/PKB with Reduced PI3K Activation in Insulin-resistant Mice, Am J Physiol/Endo 281, E1249, 2001
Mouse Mouse, B6D2F1, F1 hybrids, New Zealand black female & New Zealand white male Vascular endothelial Collagenase: 0.2%
 PBS Launder, T., Gegen, N., Knedler, A., and Harbeck, R.: The Isolation and Characterization of Enriched Microvascular Endothelial Cells From Mouse Adipose Tissue, J Immunol Methods 102, 45, 1987
Porcine Porcine, female, <1 year Adipose mesenchymal stem Collagenase Type 1: 0.1%
 DMEM Williams, K., Picou, A., Kish, S., Giraldo, A., Godke, R. and Bondioli, K: Isolation and Characterization of Porcine Adipose Tissue-Derived Adult Stem Cells., Cells Tissues Organs 188, 251, 2008
Porcine Porcine, crossbred, male 1-4 day Adipocytes Collagenase Type 1: 0.2%
 DMEM/F12 Ramsay, T.G.: Porcine Leptin Inhibits Lipogenesis in Porcine Adipocytes, J Anim Sci 81, 3008, 2003
Porcine Porcine, 8-9 week Adipose Collagenase Type 1: 300 u/ml
 HEPES Ding, S., McNeel, R., and Mersmann, H.: Expression of Porcine Adipocyte Transcripts: Tissue Distribution and Differentiation In Vitro and In Vivo, Comp Biochem Physiol B 123, 307, 1999
Porcine Porcine, neonatal Adipocytes Collagenase Type 1: 0.3%
 Krebs-Ringer bicarbonate albumin Wang Y, Fried SK, Petersen RN, Schoknecht PA: Somatotropin regulates adipose tissue metabolism in neonatal swine, J Nutr 129, 139-45, 1999
Porcine Porcine, crossbred, 1-3 day Adipose, Stromal-vascular Collagenase Type 1: 0.2%
 DMEM/F12 Suryawan, A., Swanson, L., and Hu, C.: Insulin and Hydrocortisone, But Not Triiodothyronine, Are Required for the Differentiation of Pig Preadipocytes in Primary Culture, J Anim Sci 75, 105, 1997
Rat Rat, Lewis, male Renal adipose derived cells Collagenase Type 1: 0.3%
 DMEM Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney.,Lipids Health Dis Vol. 10,, , 171, 2011
Rat Rat, SD, neonatal Brown adipocytes Collagenase Type 4: 0.1%
Neutral Protease: 0.1%
Trypsin: 0.05%
 PBS Liu, Z., Wang, H., Zhang, Y., Zhou, J., Lin, Q., Wang, Y., Duan, C., Wu, K. and Wang, C.: Efficient Isolation of Cardiac Stem Cells from Brown Adipose.,J Biomed Biotechnol Vol. 2010,, , 104296, 2010
Rat Rat, Wistar, 4 week Adipocytes Collagenase: 0.2%
 Ham's F12 Aoki, S., Toda, S., Sakemi, T., and Sugihara, H.: Coculture of Endothelial Cells and Mature Adipocytes Actively Promotes Immature Preadipocyte Development In Vitro, Cell Struct Funct 28, 55, 2003
Rat Rat, SD Adipocytes Collagenase Type 1: 0.2%
 KRHB Mora, S., Yang, C., Ryder, J., Boeglin, D and Pessin, J: The MEF2A and MEF2D Isoforms are Differentially Regulated in Muscle and Adipose Tissue during States of Insulin Deficiency, Endocrinology 142, 1999, 2001
Rat Rat, SD, male, 4-7 weeks Brown adipocytes Deoxyribonuclease I: 0.5%
 DMEM Omatsu-Kanbe, M., and Matsuura, H.: Inhibition of Store-operated Ca2+ Entry by Extracellular ATP in Rat Brown Adipocytes, J Physiol 521 (3), 601, 1999
Rat Rat Adipocytes Collagenase Type 2: 0.2%
 DMEM /F-12 Serrero, G: Primary Culture in Defined Medium of Adipocyte Precursors,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 11B:6.1, 1995
Rat Rat, SD, male, 130-160 g Adipose
Epididymal fat pads		 Collagenase: 0.3%
 Kreb's-Ringer bicarbonate buffer modified Charron, M.J. and Kahn, B.B.: Divergent Molecular Mechanisms for Insulin-Resistant Glucose Transport in Muscle and Adipose Cells In Vivo, J Biol Chem 265, 7994, 1990
Rat Rat, 3 day Preadipocytes Collagenase Type 3: 0.10%
 Parker Medium 199 Gaben-Cogneville, A., Poussin, B., Chamblier, M., Forgue-Fafitte, M., and Rosselin, G.: Development of Insulin and Epidermal Growth Factor Receptors During the Differentiation of Rat Preadipocytes in Primary Culture, Biochim Biophys Acta 968, 231, 1988
Rat Rat, SD, various weights and ages Brown adipocytes
Interscapular & cervical depots		 Collagenase: 0.2%
Soybean Trypsin Inhibitor: 0.3%
 Krebs Ringer bicarbonate buffer Woodward, Julie A. and Saggerson, E.: Effect of Adenosine Deaminase, N6-Phenylisopropyladenosine and Hypothroidism on the Responsiveness of Rat Brown Adipocytes to Noradrenaline, Biochem J 238, 395, 1986
Rat Rat, CD, male, 150-200 g Adipocytes
Epididymal fat pads		 Collagenase: 0.1%
 Krebs Ringer bicarbonate buffer Pessin, J.E., Gitomer, W., Oka, Y., Oppenheimer, C.L., and Czech, M.P.: B-Adrenergic Regulation of Insulin and Epidermal Growth Factor Receptors in Rat Adipocytes, J Biol Chem 258, 7386, 1983
Rat Rat, Wistar, albino, male, 100-140 g Adipocytes
Epididymal-fat pads		 Collagenase: 0.3%
 Kreb's Ringer Green, A. and Newsholme, E.: Sensitivity of Glucose Uptake and Lipolysis of White Adipocytes of the Rat to Insulin and Effects of Some Metabolites, Biochem J 180, 365, 1979
Rat Rat, Fischer, 344, male, 9 - 13 week White fat Collagenase: 0.3%
 Kreb's Ringer bicarbonate buffer Stiles, J.W., Francendese, A.A. and Masoro, E.J.: Influence of Age on Size an Number of Fat Cells in the Epididymal Depot, Am J Physiol 229, 1561, 1975
Rat Rat, Wistar, male, 5 week-16 month White fat Collagenase: 0.05%
 Kreb's Ringer bicarbonate buffer DiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species, Am J Physiol 221, 850, 1971
Rat Rat (CFE), albino, female Brown fat Collagenase Type 1: 0.10%
 Bicarbonate buffer Fain, J., Reed, N., and Saperstein, R.: Isolation and Metabolism of Brown Fat Cells, J Biol Chem 242, 1887, 1967
Rat Rat, SD, male, 160-210 g Fat Collagenase: 0.3%
 Albumin-bicarbonate buffer Rodbell, M.: Metabolism of Isolated Fat Cells. I. Effects of Hormones on Glucose Metabolism and Lipolysis, J Biol Chem 239 (2), 375, 1964