Stem - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Canine Canine, 20-25 kg Adipose stem cells Collagenase: see reference
 Media-199 Fischer, L., McIlhenny, S., Tulenko, T., Golesorkhi, N., Zhang, P., Larson, R., Lombardi, J., Shapiro, I. and DiMuzio, P.: Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force., J Surg Res 152, 157, 2009
Equine Equine Adipose derived stem cells Collagenase Type 1: 0.1%
 PBS Vidal, M., Robinson, S., Lopez, M., Paulsen, D., Borkhsenious, O., Johnson, J., Moore, R. and Gimble, J.: Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow.,Vet Surg Vol. 37,, , 713, 2008
Equine Equine, 1-5 year Adipose derived stem cells Collagenase Type 1: 0.1%
 PBS Vidal, M., Kilroy, G., Lopez, M., Johnson, J., Moore, R. and Gimble, J.: Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells.,Vet Surg Vol. 36,, , 613, 2007
Human Human Dental pulp derived stem cell Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
 DMEM Sakai, K., Yamamoto, A., Matsubara, K., Nakamura, S., Naruse, M., Yamagata, M., Sakamoto, K., Tauchi, R., Wakao, N., Imagama, S., Hibi, H., Kadomatsu, K., Ishiguro, N. and Ueda, M: Human Dental Pulp-Derived Stem Cells Promote Locomotor Recovery After Complete Transection of the Rat Spinal Cord by Multiple Neuro-Regenerative Mechanisms., J Clin Invest 122, 80, 2012
Human Human, male 40-60 years Adipose derived stem cells Collagenase: 0.25%
Deoxyribonuclease I: 0.002%
 PBS Blasi, A., Martino, C., Balducci, L., Saldarelli, M., Soleti, A., Navone, S., Canzi, L., Cristini, S., Invernici, G., Parati, E. and Alessandri, G.: Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential,Vasc Cell Vol. 3,, , 5, 2011
Human Human Umbilical cord stromal stem Collagenase Type 4: 0.08%
Neutral Protease: 0.138%
Hyaluronidase: 0.02%
 DMEM Farias, V., Linares-Fernandez, J., Penalver, J., Paya Colmenero, J., Ferron, G., Duran, E., Fernandez, R., Olivares, E., O'Valle, F., Puertas, A., Oliver, F. and Ruiz de Almodovar, J.: Human Umbilical Cord Stromal Stem Cell Express CD10 and Exert Contractile Properties., Placenta 32, 86, 2011
Human Human Glioma stem cells PDS kit: per instructions
 neurobasal medium Hjelmeland, A., Wu, Q., Wickman, S., Eyler, C., Heddleston, J., Shi, Q., Lathia, J., Macswords, J., Lee, J., McLendon, R. and Rich, J.: Targeting A20 Decreases Glioma Stem Cell Survival and Tumor Growth.,PLoS Biol Vol. 8,, , e1000319, 2010
Human Human Dental pulp and apical papilla stem cells Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
 MEM Huang, G., Yamaza, T., Shea, L., Djouad, F., Kuhn, N., Tuan, R. and Shi, S.: Stem/Progenitor Cell-Mediated De Novo Regeneration of Dental Pulp with Newly Deposited Continuous Layer of Dentin in an In Vivo Model.,Tissue Eng Part A Vol. 16,, , 605, 2010
Human Human Corneal stromal stem Neutral Protease: 1.2 u/ml
Collagenase: 0.1%
 DMEM Du, Y., Roh, D., Funderburgh, M., Mann, M., Marra, K., Rubin, J., Li, X. and Funderburgh, J.: Adipose-Derived Stem Cells Differentiate to Keratocytes In Vitro., Mol Vis 16, 2680, 2010
Human Human, 40-65 year Adult human adipose stem cells Collagenase Type 2: 0.075%
 DMEM Sun, N., Panetta, N., Gupta, D., Wilson, K., Lee, A., Jia, F., Hu, S., Cherry, A., Robbins, R., Longaker, M. and Wu, J.: Feeder-Free Derivation of Induced Pluripotent Stem Cells from Adult Human Adipose Stem Cells., Proc Natl Acad Sci U S A 106, 15720, 2009
Human Human Filum terminale neural progenitor Trypsin: see reference
 DMEM/F-12 Varghese, M., Olstorn, H., Berg-Johnsen, J., Moe, M., Murrell, W. and Langmoen, I.: Isolation of Human Multipotent Neural Progenitors from Adult Filum Terminale.,Stem Cells Dev Vol. 18,, , 603, 2009
Human Human, male Spermatogonial stem cells Collagenase: 1%
Deoxyribonuclease I: 0.22%
Trypsin: 0.4%
 DMEM Kossack, N., Meneses, J., Shefi, S., Nguyen, H., Chavez, S., Nicholas, C., Gromoll, J., Turek, P. and Reijo-Pera, R.: Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells., Stem Cells , , 2008
Human Human Muscle derived multiprogenitor cells Collagenase Type 2: 0.05%
 DMEM Nesti, L., Jackson, W., Shanti, R., Koehler, S., Aragon, A., Bailey, J., Sracic, M., Freedman, B., Giuliani, J. and Tuan, R.: Differentiation Potential of Multipotent Progenitor Cells Derived from War-Traumatized Muscle Tissue.,J Bone Joint Surg Am Vol. 90,, , 2390, 2008
Human Human Adipose derived stem cells Collagenase Type 1: 0.075%
 DMEM Jeong, J.: Adipose Stem Cells as a Clinically Available and Effective Source of Adult Stem Cell Therapy, Int J Stem Cells 1, 43, 2008
Human Human Hepatic stem cells and heptoblasts Collagenase Type 4: 0.014-0.06%
 various Wauthier, E., Schmelzer, E., Turner, W., Zhang, L., LeCluyse, E., Ruiz, J., Turner, R., Furth, M., Kubota, H., Lozoya, O., Barbier, C., McClelland, R., Yao, H., Moss, N., Bruce, A., Ludlow, J. and Reid, L.: Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance., Methods Cell Biol 86, 137, 2008
Human Human Bone marrow derived MSC Trypsin: 0.05%
Papain: 0.0025%
 DMEM Welter Jean F, Solchaga Luis A, Penick Kitsie J: Simplification of aggregate culture of human mesenchymal stem cells as a chondrogenic screening assay, Biotechniques 42, 732, 734-7, 2007
Human Human, 8-12 year Tendon stem/progenitor Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
 DMEM Bi Yanming, Ehirchiou Driss, Kilts Tina M, Inkson Colette A, Embree Mildred C, Sonoyama Wataru, Li Li, Leet Arabella I, Seo Byoung-Moo, Zhang Li, Shi Songtao, Young Marian F: Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche, Nat Med 13, 1219-27, 2007
Human Human, male Adipose derived adult stem cells Collagenase Type 1: 0.1%
 DMEM Lei, L., Liao, W., Sheng, P., Fu, M., He, A. and Huang, G.: Biological Character of Human Adipose-Derived Adult Stem Cells and Influence of Donor Age on Cell Replication in Culture.,Sci China C Life Sci Vol. 50,, , 320, 2007
Human Human Pancreatic cancer stem cells Collagenase Type 4: 200 u/ml
 medium 199 Li, C., Heidt, D., Dalerba, P., Burant, C., Zhang, L., Adsay, V., Wicha, M., Clarke, M. and Simeone, D.: Identification of Pancreatic Cancer Stem Cells., Cancer Res 67, 1030, 2007
Human Human, adult Adipose-derived adult stem Collagenase Type 1: 0.1%
 DMEM/F-12 Ham's Mitchell James B, McIntosh Kevin, Zvonic Sanjin, Garrett Sara, Floyd Z Elizabeth, Kloster Amy, Di Halvorsen Yuan, Storms Robert W, Goh Brian, Kilroy Gail, Wu Xiying, Gimble Jeffrey M: Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers, Stem Cells 24, 376-85, 2006
Human Human Mesenchymal stem Collagenase Type 1: 0.075%
 DMEM Kern Susanne, Eichler Hermann, Stoeve Johannes, Kluter Harald, Bieback Karen: Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue, Stem Cells 24, 1294-301, 2006
Human Human Placental mesenchymal stem Collagenase Type 2: 270 u/ml
Neutral Protease: 2.4 u/ml
 MEM Portmann-Lanz CBettina, Schoeberlein Andreina, Huber Alexander, Sager Ruth, Malek Antoine, Holzgreve Wolfgang, Surbek DanielV: Placental mesenchymal stem cells as potential autologous graft for pre- and perinatal neuroregeneration, Am J Obstet Gynecol 194, 664-73, 2006
Human Human Stromal stem cells Collagenase Type 1: 0.2%
 HBSS Boquest Andrew C, Shahdadfar Aboulghassem, Brinchmann Jan E, Collas Philippe: Isolation of stromal stem cells from human adipose tissue, Methods Mol Biol 325, 35-46, 2006
Human Human, female Adipose derived adult stem cells Collagenase Type 1: 0.1%
 DMEM/F-12 Guilak, F., Lott, K., Awad, H., Cao, Q., Hicok, K., Fermor, B. and Gimble, J.: Clonal Analysis of the Differentiation Potential of Human Adipose-Derived Adult Stem Cells., J Cell Physiol 206, 229, 2006
Human Human Adult stem cells Collagenase Type 1: 0.1%
 PBS Devireddy, R., Thirumala, S. and Gimble, J.: Cellular Response of Adipose Derived Passage-4 Adult Stem Cells to Freezing Stress., J Biomech Eng 127, 1081, 2005
Human Human, female Adipose derived adult stem cells Collagenase Type 1: 0.1%
 DMEM-Ham's F-12 Aust, L., Devlin, B., Foster, S., Halvorsen, Y., Hicok, K., du Laney, T., Sen, A., Willingmyre, G. and Gimble, J.: Yield of Human Adipose-Derived Adult Stem Cells from Liposuction Aspirates.,Cytotherapy Vol. 6,, , 7-14, 2004
Human Human, adult, male Human epidermal keratinocyte stem cells Neutral Protease: 0.5%
 DMEM Papini S, Cecchetti D, Campani D, Fitzgerald W, Grivel J, Chen S, Margolis L, Revoltella R.: Isolation and Clonal Analysis of Human Epidermal Keratinocyte Stem Cells in Long-term Culture, Stem Cells 21, 481, 2003
Human Human Umbilical vein mesenchymal stem cells Collagenase: 1%
 PBS Covas DT, Siufi JL C, Silva AR L, Orellana MD: Isolation and culture of umbilical vein mesenchymal stem cells, Braz J Med Biol Res 36, 1179-83, 2003
Human Human, 7-8 yr Stem cells Human exfoliated deciduous teeth Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
 see reference Miura, M., Gronthos, S, Zhao, M, Lu, B, Fisher, L, Robey, P, and Shi, S.: SHED: Stem Cells from Human Exfoliated Deciduous Teeth, Proc Natl Acad Sci U S A 100, 5807, 2003
Human Human, fetal Epithelial progenitor Collagenase: 0.03%
 DMEM Malhi, H., Irani, A., Gagandeep, S. and Gupta, S.: Isolation of Human Progenitor Liver Epithelial Cells with Extensive Replication Capacity and Differentiation into Mature Hepatocytes., J Cell Sci 115, 2679, 2002
Human Human, adult Human skin mast cells Collagenase Type 2: 0.15%
Hyaluronidase: 0.07%
Deoxyribonuclease I: 0.03%
 HBSS Kambe, N, Kambe, M, Kochan, J, and Schwartz ,L.: Human Skin-derived Mast Cells Can Proliferate While Retaining Their Characteristic Functional and Protease Phenotypes, Blood 97, 2045, 2001
Human Human Central nervous system stem Collagenase: 0.1%
Hyaluronidase: 0.1%
 HBSS Uchida, N., Buck, D., He, D., Reitsma, M., Masek, M., Phan, T., Tsukamoto, A., Gage, F. and Weissman, I.: Direct Isolation of Human Central Nervous System Stem Cells., Proc Natl Acad Sci U S A 97, 14720, 2000
Human Human Embryonic stem Neutral Protease: 1%
Collagenase Type 4: 0.1%
 DMEM Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM: Embryonic stem cell lines derived from human blastocysts, Science 282, 1145-7, 1998
Human Human Muscle-derived stem cells Trypsin: 0.25%
 DMEM/F12 Alessandri Giulio, Pagano Stefano, Bez Alessandra, Benetti Anna, Pozzi Stefano, Iannolo Gioacchin, Baronio Manuela, Invernici Gloria, Caruso Arnaldo, Muneretto Claudio, Bisleri Gianluigi, Parati Eugenio: Isolation and culture of human muscle-derived stem cells able to differentiate into myogenic and neurogenic cell lineages, Lancet 364, 1872-83,
Monkey Monkey Embryonic stem Collagenase Type 4: 0.08%
 DMEM Chen, S., Revoltella, R., Papini, S., Michelini, M., Fitzgerald, W., Zimmerberg, J.,and Margolis, L.: Multilineage Differentiation of Rhesus Monkey Embryonic Stem cells in Three-dimensional Culture Systems, Stem Cells 21(3), 281, 2003
Monkey Baboons, 1yr, 7yr, 14 yr Primate spermatogonial Collagenase Type 2: 0.1%
Trypsin: 0.05%
Deoxyribonuclease I: 0.1%
 DMEM Nagano, M., McCarrey, J., and Brinster, R.: Primate Spermatogonial Stem Cells Colonize Mouse Testes, Biol Reprod 64, 1409, 2001
Mouse Mouse, 8-10 week Adipose derived stem Collagenase Type 1: 0.025%
 HBSS Sugii, S., Kida, Y., Berggren, W. and Evans, R.: Feeder-Dependent and Feeder-Independent iPS Cell Derivation from Human and Mouse Adipose Stem Cells., Nat Protoc 6, 346, 2011
Mouse Mouse Spleen, bone marrow endothelial Collagenase Type 4: 0.3-1.0%
Deoxyribonuclease I: 20 u/ml
 PBS Shi, C., Jia, T., Mendez-Ferrer, S., Hohl, T., Serbina, N., Lipuma, L., Leiner, I., Li, M., Frenette, P. and Pamer, E.: Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands., Immunity 34, 590, 2011
Mouse Mouse Stem and progenitor Collagenase Type 2: 0.2%
 HBSS Han, J., Koh, Y., Moon, H., Ryoo, H., Cho, C., Kim, I. and Koh, G.: Adipose Tissue is an Extramedullary Reservoir for Functional Hematopoietic Stem and Progenitor Cells., Blood 115, 957, 2010
Mouse Mouse, 6-8 week Bone marrow mesenchymal stem Collagenase Type 1: 0.25%
 RPMI 1640 Xu, S., De Becker, A., Van Camp, B., Vanderkerken, K. and Van Riet, I.: An Improved Harvest and In Vitro Expansion Protocol for Murine Bone Marrow-Derived Mesenchymal Stem Cells.,J Biomed Biotechnol Vol. 2010,, , 105940, 2010
Mouse Mouse, adult Adult neural stem Trypsin: 0.05%
 DMEM Deleyrolle, L. and Reynolds, B.: Isolation, Expansion, and Differentiation of Adult Mammalian Neural Stem and Progenitor Cells using the Neurosphere Assay., Methods Mol Biol 549, 91, 2009
Mouse Mouse, 6-8 week Prostate epithelial/stem Collagenase Type 2: 0.5%
Trypsin: 0.05%
 HBSS Burger, P., Gupta, R., Xiong, X., Ontiveros, C., Salm, S., Moscatelli, D. and Wilson, E.: High Aldehyde Dehydrogenase Activity: A Novel Functional Marker of Murine Prostate Stem/Progenitor Cells., Stem Cells 27, 2220-8, 2009
Mouse Mouse Germ cells Collagenase Type 1: 100 u/ml
 HBSS Breault, D., Min, I., Carlone, D., Farilla, L., Ambruzs, D., Henderson, D., Algra, S., Montgomery, R., Wagers, A. and Hole, N.: Generation of mTert-GFP Mice as a Model to Identify and Study Tissue Progenitor Cells., Proc Natl Acad Sci U S A 105, 10420, 2008
Mouse Mouse Neural progenitor cell Papain: 10 ul/ml
 PBS Hutton, S. and Pevny, L.: Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System., Cold Spring Harb. Protoc. 11, 5077, 2008
Mouse Mouse, embryonic HES-BC cells Trypsin: 0.05%
 DMEM Lu Shi-Jiang, Feng Qiang, Caballero Sergio, Chen Yu, Moore Malcolm A, Grant Maria B, Lanza Robert: Generation of functional hemangioblasts from human embryonic stem cells, Nat Methods 4, 501-9, 2007
Mouse Mouse, 6-8 week Tendon stem/progenitor Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
 DMEM Bi Yanming, Ehirchiou Driss, Kilts Tina M, Inkson Colette A, Embree Mildred C, Sonoyama Wataru, Li Li, Leet Arabella I, Seo Byoung-Moo, Zhang Li, Shi Songtao, Young Marian F: Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche, Nat Med 13, 1219-27, 2007
Mouse Mouse Liver epithelial progenitor cells Collagenase Type 4: 0.1%
Deoxyribonuclease I: 0.05%
 DMEM Li Wen-Lin, Su Juan, Yao Yu-Cheng, Tao Xin-Rong, Yan Yong-Bi, Yu Hong-Yu, Wang Xin-Min, Li Jian-Xiu, Yang Yong-Ji, Lau Joseph T Y, Hu Yi-Ping: Isolation and characterization of bipotent liver progenitor cells from adult mouse, Stem Cells 24, 322-32, 2006
Mouse Mouse, 6 week Adipose mesenchymal stem Collagenase: 0.2%
 PBS Di Rocco Giuliana, Iachininoto Maria Grazia, Tritarelli Alessandra, Straino Stefania, Zacheo Antonella, Germani Antonia, Crea Filippo, Capogrossi Maurizio C: Myogenic potential of adipose-tissue-derived cells, J Cell Sci 119, 2945-52, 2006
Mouse Mouse Neural stem cells Papain: see reference
 DMEM/F-12 Meletis, K., Wirta, V., Hede, S., Nister, M., Lundeberg, J. abd Frisen, J.: p53 Suppresses the Self-Renewal of Adult Neural Stem Cells., Development 133, 363, 2006
Mouse Mouse, day 7 Cerebellar stem cells Papain: 10u/ml
Deoxyribonuclease I: 250 u/ml
 Dulbecco's PBS Lee, A, Kessler, J, Read, T, Kaiser, C, Corbeil, D, Huttner ,W, Johnson, J, Wechsler-Reya, R.: Isolation of Neural Stem Cells from the Postnatal Cerebellum, Nat Neurosci 8, 723, 2005
Mouse Mouse, embryonic Embryonic fibroblast feeder cells Collagenase Type 4: 0.1%
 DMEM Schatten Gerald, Smith Joseph, Navara Christopher, Park Jong-Hyuk, Pedersen Roger: Culture of human embryonic stem cells, Nat Methods 2, 455-63, 2005
Mouse Mouse, 1 day Neural progenitor PDS kit: per instructions
 see reference Seaberg, R., Smukler, S. and Van der Kooy, D.: Intrinsic Differences Distinguish Transiently Neurogenic Progenitors from Neural Stem Cells in the Early Postnatal Brain., Dev Biol 278, 71, 2005
Mouse Mouse, 8-10 week Liver derived stem cells Collagenase Type 1: 0.1%
Neutral Protease: 2.4 u/ml
 HBSS Kotton, D., Fabian, A. and Mulligan, R.: A Novel Stem-Cell Population in Adult Liver with Potent Hematopoietic-Reconstitution Activity., Blood 106, 1574, 2005
Mouse Mouse Bone marrow Collagenase Type 1: 0.15%
Neutral Protease: 0.15%
 PBS Bertoncello, I. and Williams, B.: Hematopoietic Stem Cell Characterization by Hoechst 33342 and Rhodamine 123 Staining., Methods Mol Biol 263, 181, 2004
Mouse Mouse, 4-7 day Pluripotent stem cells Collagenase Type 1: 220 u/ml
Neutral Protease: 33 u/ml
 MEM Howell, J., Lee, W., Morrison, P., Zhong, J., Yoder, M. and Srour, E.: Pluripotent Stem Cells Identified in Multiple Murine Tissues., Ann N Y Acad Sci 996, 158, 2003
Mouse Mouse, 15 day or 3 month Neural stem cells Papain: 0.1%
 DMEM/F-12 Gritti, A, Bonfanti, L, Doetsch, F, Caille, I, Alvarez-Buylla, A, Lim, D, Galli, R, Verdugo J, Herrera, D, and Vescovi A.: Multipotent Neural Stem Cells Reside into the Rostral Extension and Olfactory Bulb of Adult Rodents, J Neurosci 22(2), 437, 2002
Mouse Mouse, embryonic Cortical progenitors PDS kit: per instructions
 Serum free medium Estivill-Torrus, G., Pearson, H., Van Heyningen, V., Price, D. and Rashbass, P.: Pax6 is Required to Regulate the Cell Cycle and the Rate of Grogression from Symmetrical to Asymmetrical Division in Mammalian Cortical Progenitors., Development 129, 455, 2002
Mouse Mouse, 4-8 month Neural subventricular zone Trypsin: 0.13%
Hyaluronidase: 0.067%
 DMEM/F12 Gritti A, Frolichsthal-Schoeller P, Galli R, Parati E, Cova L, Pagano S, Bjornson C, and Vescovi A.: Epidermal and Fibroblast Growth Factors Behave as Mitogenic Regulators for a Single Multipotent Stem Cell-like Population from the Subventricular Region of the Adult Mouse forebrain, J Neurosci 19(9), 3287, 1999
Mouse Mouse, 6 week Muscle hematopoietic stem cells Collagenase: 0.2%
Trypsin: 0.1%
 DMEM Jackson, K., and Goodell, M.: Hematopoietic Potential of Stem Cells Isolated from Murine Skeletal Muscle, Proc Natl Acad Sci U S A 96, 14482, 1999
Porcine Porcine, female, <1 year Adipose mesenchymal stem Collagenase Type 1: 0.1%
 DMEM Williams, K., Picou, A., Kish, S., Giraldo, A., Godke, R. and Bondioli, K: Isolation and Characterization of Porcine Adipose Tissue-Derived Adult Stem Cells., Cells Tissues Organs 188, 251, 2008
Rabbit Rabbit, New Zealand, 8-10 week Tenocytes and tendon stem cells Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
 DMEM Zhang, J. and Wang, J.: Characterization of Differential Properties of Rabbit Tendon Stem Cells and Tenocytes., BMC Musculoskelet Disord 11, 10, 2010
Rat Rat, SD, newborn Neutral stem cells, Schwann cells Trypsin: 0.25%
Collagenase: 0.16%
 DMEM/F12 Zeng Yuan-Shan, Ding Ying, Wu Li-Zhi, Guo Jia-Song, Li Hai-Biao, Wong Wai-Man, Wu Wu-Tian: Co-transplantation of schwann cells promotes the survival and differentiation of neural stem cells transplanted into the injured spinal cord, Dev Neurosci 27, 20-6, 2005
Rat Rat, male, 200-250 g Neural stem cells Papain: 0.09%
Deoxyribonuclease I: 0.1%
 EBSS Gobbel GT, Choi SJ, Beier S, Niranjan A: Long-term cultivation of multipotential neural stem cells from adult rat subependyma, Brain Res 980, 221, 2003
Rat Rat, adult Neural stem cells Papain: 2.5 u/ml
Deoxyribonuclease I: 250 u/ml
Neutral Protease: 1 u/ml
 DMEM/F-12 Palmer, T., Markakis, E., Willhoite, A., Safar, F., and Gage, F.: Fibroblast Growth Factor-2 Activates a Latent Neurogenic Program in Neural Stem Cells from Diverse Regions of the Adult CNS, J Neurosci 19, 8487, 1999
Rat Rat Central nervous system stem cells Papain: 0.01%
Neutral Protease: 0.1%
 DMEM/F-12 Ray, J. and Gage, F.: Neural Stem Cell Isolation, Characterization and Transplantation,Modern Techniques in Neuroscience Research,Johansson, H. and Windhorst, U., Springer-Verlag New York, 339, 1999
Rat Rat, embryonic Neurons and progenitor Papain: 20 u/ml
 EBSS Maric, D., Maric, I. and Barker, J.: Buoyant Density Gradient Fractionation and Flow Cytometric Analysis of Embryonic Rat Cortical Neurons and Progenitor Cells.,Methods Vol. 16,, , 247, 1998