Phosphatase, Alkaline Product Information
I.U.B.: 3.1.3.1
Alkaline phosphatase is a broad term associated with non-specific phosphomonoesterases with an alkaline pH optimum.
Source/Unit Definitons:
CAP: Calf intestine. One Worthington Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 37°C, pH 9.8.
BAPF, BAPC, BAPSF: E. coli. One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.
PC: Chicken Intestine. One Unit hydrolyzes 1µmole of o-carboxyphenol phosphate per minute at 25°C, pH 8.8.
Technical Notes: Worthington chicken intestine alkaline phosphatase (Code: PC) is the preparation used in the NF/USP dexamethasone phosphate measurement.
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| Description | Activity | Code | Size | Cat. No. | Price | |
Phosphatase, Alkaline, Purified
Chromatographically purified, EIA grade. A solution in 50% glycerol containing 5mM MgCl2 and 0.12mM ZnCl2. Protein concentration is approximately 20mg/ml.
Store at 2-8°C.
|
≥3,000 units per mg protein (37°C, pH9.8, DEA) |
CAP |
1 mg |
LS004228 |
$50.00 |
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| 5 mg |
LS004230 |
$210.00 |
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| Bulk |
LS004234 |
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Phosphatase, Alkaline, Purified
Chromatographically purified from Code: BAPC. Ribonuclease ≤ 0.002% by weight as RNase A using a poly C assay. Phosphodiesterase not detectable when assayed at 0.1 mg/ml with bis(p-nitrophenyl) phosphate. A suspension in 2.6M ammonium sulfate, pH 8.0.
Store at 2-8°C.
|
≥30 units per mg protein (25°C, pH 8.0) |
BAPF |
1 mg |
LS006130 |
$28.00 |
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| 5 mg |
LS006124 |
$105.00 |
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| 25 mg |
LS006123 |
$465.00 |
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| Bulk |
LS006122 |
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Phosphatase, Alkaline
Chromatographically purified. A suspension in 2.6M ammonium sulfate, pH 8.0.
Store at 2-8°C.
|
≥20 units per mg protein (25°C, pH 8.0) |
BAPC |
5 mg |
LS005129 |
$60.00 |
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| 10 mg |
LS005130 |
$97.00 |
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| Bulk |
LS005131 |
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Phosphatase, Alkaline
Partially purified. A suspension in 2.6M ammonium sulfate, pH 8.0.
Store at 2-8°C.
|
≥10 units per mg protein (25°C, pH 8.0) |
BAPSF |
10 mg |
LS004081 |
$24.00 |
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| Bulk |
LS004082 |
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Phosphatase, Alkaline
Partially purified. A dried powder. Used in the NF/USP dexamethasone phosphate assay.
Store at 2-8°C.
|
≥0.9 units per mg dry weight (25°C pH 8.8) |
PC |
250 mg |
LS003172 |
$68.00 |
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| 1 gm |
LS003171 |
$200.00 |
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| 5 gm |
LS003170 |
$800.00 |
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| Bulk |
LS003174 |
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