Chymotrypsin Product Information

Source: Bovine Pancreas

I.U.B.: 3.4.21.1

Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. Chymotrypsin catalyzes the hydrolysis of bonds of leucyl, methionyl, asparaginyl and glutamyl residues.

Stability/Storage: The enzyme is stable for days in solution at pH 3.0 and for years as a dry powder at 2-8°C. Protect from moisture.

Unit Definition: One Unit hydrolyzes one micromole of benzoyl-L-tyrosine ethyl ester per minute at 25°C, pH 7.8 in the presence of calcium. An activity of 45 units per mg using the above definition, is the equivalent of 10,000 optical density or 1330 N.F. units per mg using ATEE as a substrate.

1 BTEE unit = 29.5 USP/NF units.

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DescriptionActivityCodeSizeCat. No.Price
Chymotrypsin, Alpha, TLCK Treated, Sequence
Three time crystallized and treated with 1-chloro-3-tosylamido-7-amino-2-heptanone (TLCK) to inhibit trypsin activity [Shaw, et al., Biochemistry, 4, 2219 (1965)]. Dialyzed to remove autolysis products and low molecular weight contaminants. Supplied as lyophilized powder.
Store at 2-8C.
≥40 u/mgP CDSEQ 4x25 ug LS02130 $150.00 Buy Now
4x100 ug LS02132 $395.00 Buy Now
Chymotrypsin, Alpha, TLCK Treated
Three times crystallized and treated with 1-chloro-3-tosylamido-7-amino-2-heptanone (TLCK) to inhibit trypsin activity [Shaw, et al., Biochemistry, 4, 2219 (1965)]. Dialyzed against 1 mM HCl to remove autolysis products and low molecular weight contaminants. Supplied as a dialyzed, lyophilized powder.
Store at 2-8C.
≥45 units per mg protein CDTLCK 25 mg LS001430 $26.00 Buy Now
100 mg LS001432 $62.00 Buy Now
1 gm LS001434 $490.00 Buy Now
Bulk LS001438 Inquire
Chymotrypsin, Alpha, Purified
Chromatographically prepared by the procedure of Yapel, et al., J. Amer. Chem. Soc., 88, 2573 (1966). A lyophilized powder.
Store at 2-8C.
≥45 units per mg protein CDS 100 mg LS001475 $63.00 Buy Now
1 gm LS001479 $484.00 Buy Now
Bulk LS001477 Inquire
Chymotrypsin, Alpha
Three times crystallized alpha chymotrypsin which is an activation product of a 3X crystallized zymogen. Dialyzed against 1mM HCl and lyophilized.
Store at 2-8C.
≥45 units per mg protein CDI 250 mg LS001448 $36.00 Buy Now
1 gm LS001450 $106.00 Buy Now
10 gm LS001451 $797.00 Buy Now
Bulk LS001453 Inquire
Chymotrypsin, Alpha
Crystallized as zymogen and activated. Dialyzed against 1mM HCl and lyophilized.
Store at 2-8C.
≥35 units per mg protein CDAG 1 gm LS001333 $29.00 Buy Now
10 gm LS001334 $163.00 Buy Now
Bulk LS001332 Inquire

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