Cell Isolation Optimizing System

Worthington Biochemical Corporation offers a complete method development kit containing an assortment of enzymes most frequently used in tissue dissociation and cell isolation procedures. The "Cell Isolation Optimizing System" includes instructions, references and strategies for the handling, use and optimization of enzymatic cell isolation methods to achieve maximum yield of viable cells.

The "System" contains all of the enzymes commonly referenced in tissue dissociation and cell isolation procedures along with the Cell Isolation Guide detailing the tissue types commonly used, the mode of action of the various enzymes, tissue culture techniques, and protocol optimization guidelines. In addition the guide lists hundreds of cell and tissue specific isolation references for getting started in enzymatic cell isolation.

Kit Contents

Enzyme

Code*

Qty/Vial

Collagenase Type 1

CLS1

500 mg dw

Collagenase Type 2

CLS2

500 mg dw

Collagenase Type 3

CLS3

500 mg dw

Collagenase Type 4

CLS4

500 mg dw

Trypsin

TL

500 mg dw

Hyaluronidase

HSE

50,000 Units

Elastase

ESL

100 mg P

Papain

PAPL

100 mg P

Deoxyribonuclease I

DP

25 mg dw

Trypsin Inhibitor

SIC

100 mg dw

Neutral Protease (Dispase)

NPRO

10 mg dw

Up: Worthington Enzyme Manual