Enzymatic Reaction (image will open in a new window)
Cl. histolyticum produces two distinct types of collagenase: Collagenase 1 is very active on native collagen and synthetic substrates but with negligible activity on gelatin and azocoll, and collagenase 2 is active on gelatin but less active on synthetic substrates. Clostridiopeptidase A is a collagenase 1. It degrades the helical regions on native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Activity is measured by the specific assay of Wünsch and Heidrich. CLOSA is suitable for more critical experiments requiring only partial collagen degradation.
Worthington's clostridiopeptidase A was a highly purified form of collagenase that we no longer manufacture. For additional information please see our collagenase manual entry.
Stability: Stable for years at 2 - 8 ° C.
Storage: Store at 2-8 ° C.
Unit Definition: 1 unit releases 1 micromole of p-phenylazobenzyloxycarbonyl-L-prolyl-L-leucine per minute at 25 ° C, pH 7.1.