Dextranase

Dextranases catalyze the endohydrolysis of 1,6-[[alpha]]-glucosidic linkages in dextran. They have been reviewed by Fischer and Stein (1960).

Characteristics of Dextranase from Penicillium Sp.:

Molecular weight: 41,000 (gel permeation chromatography) (Chaiet et al. 1970).

Optimum pH: From pH 5.0 to 7.0 (Chaiet et al. 1970).

Extinction coefficient: = 20.0 (Chaiet et al. 1970).

Isoelectric points: 4.55 ± 0.05 (Chaiet et al. 1970).

Activators: Co²⁺, Mn²⁺, Cu²⁺ (Sugiura et al. 1973).

Specificity: Dextranase preferentially cleaves the α-1,6 linkages of dextran (Sugiura et al. 1973).

Inhibitors: Hg²⁺, Ag²⁺, N-bromosuccinimide, I₂ (Sugiura et al. 1973).

Stability: The enzyme is stable at pH 6.0 up to 40°C (Sugiura et al. 1973).