Neutral Protease (Dispase) Product Information

Source: Bacillus polymyxa

I.U.B.: 3.4.24.28

Neutral protease (Dispase®) is a non-mammalian animal origin free (AOF) metallo, neutral protease, purified by methods developed at Worthington. Its mild proteolytic action makes the enzyme especially suitable for the preparation of primary cells and secondary (subcultivation) cell culture, since it is gentle on cell membranes. This protease is also used as a secondary enzyme in cell isolation and tissue dissociation applications, commonly used with collagense.

Characteristics of Neutral Protease (Dispase®) from Bacillus polymyxa

Molecular Weight: 36 kda.

pH Optimum: Stable over a wide pH range: 4.0-9.0, optimum pH 5.9-7.0.

Stability/Storage: Stable at 2-8°C for 12 months. Aliquot and store at -20°C after reconstitution with water or commonly used balanced salt solutions or media.

Unit Definition: One Unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C, pH 7.5, using casein as the substrate.

Specificity: Non-specific cleavage of peptide bonds containing leucine and phenylalanine.

Activators: Divalent cations including Ca2+, Mg2+, Mn2+, and Fe2+

Inhibitors: EDTA (1mM), EGTA, 1-10-phenanthroline and heavy metals

Application(s): Commonly used to separate skin epidermis from dermis leaving intact epithelial sheets and stem cell, hepatocyte and other cell isolation applications. However, due to the diversity of the variables involved, exact isolation conditions should be determined empirically for each cell/tissue application.

Related Products:

Collagenase (CLS1-4/CLSS1-4/CLSPA)
Deoxyribonuclease I (DP/D/DCLS/D2/DPFF/DPRF)
Elastase (ES/ESL/ESFF)
Hyaluronidase (HSE/HSEP)
Papain (PAP/PAPL/PAP2)
Proteinase K (PROK)
Trypsin (TL/TRL/TRL3/TRLS/TRTPCK)
Trypsin Inhibitors (LBI/OI/SI/SIC)

Cell Isolation Optimizing System (CIT)
Hepatocyte Isolation System (HIS)
Neonatal Cardiomyocyte Isolation System (NCIS)
Papain Dissociiation System (PDS/PDS2)

DescriptionActivityCodeSizeCat. No.Price
Neutral Protease (Dispase), Purified
Chromatographically purified. A lyophilized powder.
Store at 2-8C.
≥4 units per mg dry weight NPRO 10 mg LS02100 $70.00 Buy Now
50 mg LS02104 $295.00 Buy Now
Bulk LS02108 Inquire
Neutral Protease, Partially Purified
Partially purified. A lyophilized powder.
Store at 2-8C.
≥0.1 units per mg dry weight NPRO2 1 gm LS02109 $142.00 Buy Now
5 gm LS02111 $640.00 Buy Now
Bulk LS02112 Inquire

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