Worthington offers DNA isolated from a number of different sources:
Calf thymus:
(Code: DNA) Prepared and purified by a method developed at Worthington to have lower protein and RNA contamination than most other commercial preparations. This highly polymerized DNA is an excellent substrate for deoxyribonuclease. A sodium salt, it must be converted to magnesium form to be susceptible to DNase.
Salmon Testes:
(Code: SDNA) Prepared by a modification of the method of Emanuel and Chaikoff, (1953). A minimum of 75% native nucleic acid.
Deoxyribonucleic acid, denatured fragmented: (Code: SDNAD) Prepared from purified salon testes DNA, fragmented by mechanical shearing and denatured by heat. Average size of fragments 200 - 1,000 base pairs.
Escherichia coli:
(Code: DNAEC) Isolated as described by Marmur (1961).
E. coli W8850 (lambda Cl857s7)
(Code: DNAL) is prepared from CsCl purified phage and is purified to an A260/A280 greater than 1.8. Homogeneous in agarose gel electrophoresis. Supplied as a solution in 10mM Tris⋅HCl pH 8, with 1 mM EDTA. One A260 unit is equivalent to 50 ug DNA.
Storage: Storage buffer 10 mM Tris⋅HCl, pH 8.0 containing 1 mM EDTA. Store at - 20°C. Once thawed keep at 2-8°C.
Deoxyribonucleic Acids, Cellulose
Cellulose, Calf Thymus DNA
DNA cellulose is suitable for the chromatography of many proteins that are normally associated with DNA including DNA polymerase, RNA polymerase, many nucleases, and DNA binding proteins. When properly washed the cellulose contains 3 - 4 mg calf thymus DNA per gram. It is prepared by the method of Alberts and Herrick (1970).
Cellulose, ds, Calf Thymus DNA
Microcrystalline cellulose containing bound native calf thymus DNA, prepared according to a method developed at Worthington is by which native calf thymus DNA is covalently bound to cellulose via free hydroxyl groups. Suitable for the affinity purification of many proteins associated with nucleic acids, including DNA/RNA polymerases, exonucleases, and DNA binding proteins. One gram of dsDNA-cellulose contains a minimum of 3 mg native DNA, and when fully hydrated swells to 3 ml - 4 ml gel.
Microcrystalline cellulose containing bound denatured calf thymus DNA is prepared according to a method developed at Worthington by which denatured calf thymus DNA is covalently bound to cellulose via free hydroxyl groups. Suitable for the affinity purification of many proteins associated with nucleic acids, including DNA/RNA polymerases, exonucleases, reverse transcriptase, and DNA binding proteins. One gram of ssDNA-cellulose contains a minimum of 3 mg SS-DNA, and when fully hydrated swells to 3 ml - 4 ml gel.
Deoxyribonuclease Acid, Markers
Lambda DNA BstE II Fragments
A mixture of DNA fragments is prepared by digesting λDNA (Code: DNAL) with restriction endonuclease BstEII. The digest can be used as DNA molecular weight markers. On agarose gel electrophoresis the mixture separates into 14 individual bands having the following number of base pairs: 8454 bp, 7242 bp, 6369 bp, 5686 bp, 4822 bp, 3775 bp, 2323 bp, 1929 bp, 1371 bp, 1264 bp, 702 bp, 224 bp, 117 bp.
Lambda DNA EcoR I Fragments
A mixture of DNA fragments is obtained by digestion of λDNA (Code: DNAL) with restriction endonuclease EcoR I. The digest can be used as DNA molecular markers. On agarose gel elecrophoresis the mixture separates into 5 individual bands having the following number of base pairs: 21226 bp, 7421bp, 5804 bp, 4878 bp, and 3530 bp
Lambda DNA Hind III Fragments
A mixture of DNA fragments obtained by digestion of λDNA (Code: DNAL) with restriction endonuclease Hind III. The digest can be used as DNA molecular weight markers. On agarose gel elecrophoresis the mixture separates into 7 individual bands having the following number of base pairs: 23130 bp, 9416 bp, 6557 bp, 4361bp, 2322 bp, 2027 bp, and 564 bp. Note: A higher sample load concentration may be required to clearly see the 564 base pair band.
Technical Note: One base pair is approximately 680 daltons. One A260 unit is equivalent to 50 ug DNA products
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