DNA Polymerase, Taq

C.A.S.: 9012-90-2

Deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase (DNA-directed)

Taq DNA polymerase is a DNA-dependent polymerase. Taq refers to Thermus aquaticus, the bacteria from which this thermostable polymerase was first isolated. It consists of a single polypeptide chain. DNA synthesis proceeds in the 5' → 3' direction. It requires the presence of dNTPs and a primer-template DNA. The enzyme is thermostable and exhibits optimal activity at 75°C.

Uses: The highly processive and thermostable Taq DNA polymerase is suitable for primer-dependent DNA labeling or DNA sequencing particularly since the reaction can proceed at high temperature (75°C).

Molecular Weight: Approximately 95 kDa

Enzymatic Reaction (image will open in a new window)

Stability:  The enzyme is thermostable and exhibits optimal activity at 75°C.  It is stable at -20°C when undiluted.  If needed, it is recommended to dilute the enzyme in storage buffer containing 0.1% nuclease-free BSA (Worthington Code: BSANF).  Storage buffer, 50 mM potassium phosphate, pH 7.4, 1 mM DTT and 15% glycerol.

Storage:  Store at -20°C.

Unit Definition:  One unit incorporates 10 nanomoles of total deoxynucleotides into acid precipitatable products with activated calf thymus DNA as template primer in 30 minutes at 37°C.