Deoxyribonuclease II Product Information
Source: Porcine SpleenI.U.B.: 3.1.22.1
Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.
Unit Definition: One Unit causes an increase in absorbance at 260 nm of 0.001 per minute at 25°C, pH 4.6 using highly polymerized DNA as substrate.
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| Description | Activity | Code | Size | Cat. No. | Price | |
Deoxyribonuclease II
A dialyzed, lyophilized powder.
Store at 2-8°C.
|
≥800 units per mg dry weight |
HDA |
80 ku |
LS002425 |
$52.00 |
|
| Bulk |
LS002427 |
Inquire |
Deoxyribonuclease II, Purified
Chromatographically purified in a modification of the procedure of Bernardi, et al., BBA, 129, 1 (1966). A dialyzed, lyophilized powder.
Store at -20°C.
REQUIRES SPECIAL SHIPPING: ICE PACK |
≥12,000 units per mg protein |
HDAC |
20 ku |
LS005410 |
$210.00 |
|
| Bulk |
LS005411 |
Inquire |
Deoxyribonuclease II, Purified, Solution
Chromatographically prepared. A solution in 50% glycerol.
Store at -20°C.
REQUIRES SPECIAL SHIPPING: ICE PACK |
≥12,000 units per mg protein |
HDACS |
2 ku |
LS005416 |
$35.00 |
|
| 5 ku |
LS005418 |
$80.00 |
|
| Bulk |
LS005420 |
Inquire |
