Deoxyribonuclease II Product Information

Source: Porcine Spleen

I.U.B.: 3.1.22.1

Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.

Unit Definition: One Unit causes an increase in absorbance at 260 nm of 0.001 per minute at 25°C, pH 4.6 using highly polymerized DNA as substrate.

Related Products:

Albumin, Nuclease-Free (BSANF)
Deoxyribonuclease I (DP/D/DCLS/D2/DPFF/DPRF)
E•RASE RNase A/T1 Blend (RCT)
Histones (H, NHL)
Lysozyme (LY/LYSF)
Nuclease, Micrococcal (NFCP)
Nuclease, S1 (SINUC/SINUCL)
Nucleic Acids, DNA, E.coli, Lambda/Fragments,RNA
Phosphatase, Alkaline (CAP/BAPF/BAPC/BAPSF/PC)Phosphodiesterase I (VPH)

Proteinase K (PROK/PROKS)
Reverse Transcriptase, Recombinant HIV (RTHIV)
Ribonuclease A (R/RAF/RASE/RS/RPDF)
Ribonuclease T1, Animal Origin Free (RT1S)
DescriptionActivityCodeSizeCat. No.Price
Deoxyribonuclease II
A dialyzed, lyophilized powder.
Store at 2-8C.
≥800 units per mg dry weight HDA 80 ku LS002425 $66.00 Buy Now
Bulk LS002427 Inquire
Deoxyribonuclease II, Purified
Chromatographically purified in a modification of the procedure of Bernardi, et al., BBA, 129, 1 (1966). A dialyzed, lyophilized powder.
Store at -20C.
REQUIRES SPECIAL SHIPPING: ICE PACK
≥12,000 units per mg protein HDAC 20 ku LS005410 $218.00 Buy Now
Bulk LS005411 Inquire
Deoxyribonuclease II, Purified, Solution
Chromatographically prepared. A solution in 50% glycerol.
Store at -20C.
REQUIRES SPECIAL SHIPPING: ICE PACK
≥12,000 units per mg protein HDACS 2 ku LS005416 $45.00 Buy Now
5 ku LS005418 $102.00 Buy Now
Bulk LS005420 Inquire

Up: Worthington Product Catalog