Enzymatic Reaction (image will open in a new window)
Hyaluronidase catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido-2-deoxy-b-D-glucose and D-glucose residues in hyaluronate.
Characteristics of Hyaluronidase from Bovine Testes:
Molecular weight: Borders and Raftery (1968) reported a molecular weight of 61,000. Khorlin et al. (1973) report four subunits of 14,000 each and a total molecular weight of 55,000.
Optimum pH: 4.5 - 6 (DeSalequi et al. 1967).
Composition: The enzyme is a glycoprotein containing 5% mannose and 2.17% glucosamine. The amino acid composition has been determined ( Borders and Raftery 1968).
Extinction coefficient: = approximately 8.
Inhibitors: Fe2+ and Fe3+ are inhibitory as are Mn2+ and Cu2+ (Warren et al. 1962).
Specificity: Testicular hyaluronidase hydrolyzes the endo-N-acetylhexosaminic bonds of hyaluronic acid and chondroitin sulfuric acids A and C (but not B), primarily to tetrasaccharide residues (Ludowieg et al. 1961). Monosaccharides are not liberated (Rappaport et al. 1951).
Stabilizers: Yang and Srivastav (1975) report that sodium chloride acts as a stabilizer.
Stability: Worthington hyaluronidase is stable for 1 - 2 years and the purified preparation for 6 - 12 months when stored at 2 -8°C.