Pectinase Product Information
Source: Aspergillus niger
Purified pectinase is a multi-component preparation highly effective in depolymerizing plant pectins with varying degrees of esterification. The product contains substantial hemicellulase, cellulase, pectinesterase and xylanase activities which together with pectin lyase and polygalacturonase work synergistically to digest plant cell wall tissues. When used with Worthington purified cellulase, purified pectinase has been found to be highly successful for generating good yields of viable protoplasts in several plant systems, e.g., corn, soybean, red beet, sunflower, tomato and citrus. In general, a concentration range of 0.1% to 0.5% pectinase (with accompanying 0.5% to 1.5% cellulase) used at 24°C to 37°C for periods of 1 to 16 hours will yield good results.
Stability/Storage: Protect from moisture. If not using entire bottle at once, weigh into single-use aliquots on arrival and store tightly covered and dessicated, at 2-8°C. Material is very hygroscopic and can become tacky and difficult to weigh if exposed to moisture.
Unit Definition: One Unit releases 1µmole of D-galacturonic acid from polygalacturonic acid per minute at 37°C, pH 5.0.
Technical Note: Pectinase is extremely hydroscopic; store dessicated to protect from moisture.
Related Products: Cellulase (CEL/CELF)