Papain Dissociation System Product Information

The Worthington Papain Dissociation System is a set of reagents intended for use in the neural cell isolation method of Huettner and Baughman (J. Neurosci., 6, 3044, 1986). The materials are designed for convenience and simplicity and are useful to the occasional user as well as the more experienced and frequent user. Each lot is use tested for performance in rat spinal neural cell isolation and this kit provides freshly prepared enzyme solutions for each dissociation.

Stability/Storage: The reagents are stable at ambient temperatures for the periods of time expected in normal shipping procedures, but the package should be refrigerated upon arrival. Contents may be stored at 2-8°C for 4-6 months before use. Store at 2-8°C.

Package Contents
The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0.3-0.4 cm3 each. For larger tissue samples prepare proportionately larger volumes of reagents at each step and combine them in the same ratio as described in the protocol.

• Vial 1: Sterile Earle's Balanced Salt Solution (EBSS) with calcium, magnesium, bicarbonate and phenol red, one vial per package, 100ml. Aliquots of this vial are used to reconstitute other vials and to prepare dilute inhibitor solution. Refrigerate between uses and equilibrate with sterile O2:CO2 before each use.

• Vial 2: Papain containing L-cysteine and EDTA, 5 x 100 unit single-use vials per package. The material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5ml of EBSS (Vial 1) yields a solution at 20 units of papain per ml in 1mM L-cysteine with 0.5mM EDTA. Brief incubation at 37°C is needed to insure full solubility and activity.

• Vial 3: Deoxyribonuclease I (DNase), 5 x 2000 unit single use vials per package.
This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5ml of EBSS (Vial 1) yields a solution at 2000 units of deoxyribonuclease per ml. Avoid vigorous mixing.

• Vial 4: Ovomucoid protease inhibitor with bovine serum albumin, one vial per package, 32ml upon reconstitution.
This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 32ml of EBSS (Vial 1) yields a solution at an effective concentration of 10mgs of ovomucoid inhibitor and 10mgs of albumin per ml. Aliquots of this vial are used for each dissociation. Refrigerate between uses and equilibrate with sterile O2:CO2 before each use. Stable after reconstitution when stored at 2-8°C.

Also included is a card correlating color with pH for use as a guide in O2:CO2 equilibration.

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DescriptionActivityCodeSizeCat. No.Price
Papain Dissociation System
Source: N/A
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's balanced salt solution (EBSS), and an inhibitor vial for use in the tissue dissociation method of Huettner, J.E., and Baughman, R.W.: J. Neuroscience, 6, 3044 (1986). Use tested by Worthington using new-born rat pup spinal cord. The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0.3-0.4 cm3 each.
Store at 2-8C.
PDS 1 bx LK003150 $240.00 Buy Now
3 bx LK003153 $635.00 Buy Now
Papain Dissociation System, Without EBSS
Source: N/A
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).
Store at 2-8C.
PDS2 1 bx LK003160 $220.00 Buy Now
3 bx LK003163 $585.00 Buy Now
PDS Kit, Papain Vial
Source: Papaya Latex
A component of the Papain Dissociation System, for use in the tissue dissociation method of Huettner, J.E., and Baughman, R.W.: J. Neuroscience, 6, 3044 (1986). Contains papain, L-cysteine, and EDTA. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of EBSS or equivalent yields a solution at 20 units of papain per ml in 1mM L-cysteine with 0.5mM EDTA.
Store at 2-8C.
≥100 units per vial PAP2 1 vi LK003176 $24.00 Buy Now
5 vi LK003178 $81.00 Buy Now
DNase Vial (D2)
Source: Bovine Pancreas
A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of EBSS or equivalent yields a solution of 2000 units/ml of deoxyribonuclease (1 mg/ml).
Store at 2-8C.
≥1,000 units per vial D2 1 vi LK003170 $23.00 Buy Now
5 vi LK003172 $78.00 Buy Now
PDS Kit, Inhibitor Vial
Source: Egg White/BSA
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and lyophilized in autoclaved vials to contain 10 mg/ml each upon reconstitution with 32 ml of EBSS.
Store at 2-8C.
≥300 mg TRL inhibited per vial OI-BSA 1 vi LK003182 $72.00 Buy Now
PDS Kit, EBSS Vial
Source: N/A
Earle's balanced salt solution (EBSS) as supplied in the Papain Dissociation System.
Store at 2-8C.
EBSS 1 vi LK003188 $51.00 Buy Now

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