Papain Dissociation System References

CAS:
9035-81-1
9048-46-8
9003-98-9
9001-73-4
Bader, C. , MacLeish, P. and Schwartz, E.
Bashor, Mark M.
, Vol. LVIII (I) , 124 , 1979
Dreyfus, Cheryl F. and Black, Ira B.
,
Methods Neurosci
2
, 10 , 1990
Huettner, J. and Baughman, R.
Lam, D.
Biosynthesis of Acetylcholine in Turtle Photoreceptors ,
Proc Natl Acad Sci U S A
69
, 1987 , 1972
Monaco, M. , Maric, D. , Bandeian, A. , Leibovitch, E. , Yang, W. and Major, E.

Papain Dissociation System Products

Description
Activity
Code
Cat. #
Size
Price
Description
Papain Dissociation System
Source:
N/A
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's balanced salt solution (EBSS), and an inhibitor vial for use in the tissue dissociation method of Huettner, J.E., and Baughman, R.W.: J. Neuroscience, 6, 3044 (1986). Use tested by Worthington using new-born rat pup spinal cord. The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0.3-0.4 cm3 each.
Store at 2-8°C.
Code
PDS
LK003150
1 bx
$295.00
LK003153
3 bx
$775.00
Description
Papain Dissociation System, Without EBSS
Source:
N/A
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).
Store at 2-8°C.
Code
PDS2
LK003160
1 bx
$265.00
LK003163
3 bx
$715.00
Description
PDS Kit, Papain Vial
Source:
Papaya Latex
A component of the Papain Dissociation System, for use in the tissue dissociation method of Huettner, J.E., and Baughman, R.W.: J. Neuroscience, 6, 3044 (1986). Contains papain, L-cysteine, and EDTA. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of EBSS or equivalent yields a solution at 20 units of papain per ml in 1mM L-cysteine with 0.5mM EDTA.
Store at 2-8°C.
Activity
≥100 units per vial
Code
PAP2
LK003176
1 vi
$29.00
LK003178
5 vi
$98.00
Description
DNase Vial (D2)
Source:
Bovine Pancreas
A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of EBSS or equivalent yields a solution of 2000 units/ml of deoxyribonuclease (1 mg/ml).
Store at 2-8°C.
Activity
≥1,000 Kunitz units per vial
Code
D2
LK003170
1 vi
$28.00
LK003172
5 vi
$92.00
Description
PDS Kit, Inhibitor Vial
Source:
Egg White/BSA
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and lyophilized in autoclaved vials to contain 10 mg/ml each upon reconstitution with 32 ml of EBSS.
Store at 2-8°C.
Activity
≥300 mg TRL inhibited per vial
Code
OI-BSA
LK003182
1 vi
$86.00