Proteinase K - Manual

Source:
Recombinant PROK produced in yeast
CAS:
39450-01-6
EC:
3.4.21.64

Proteinase K (PROK) is a serine endopeptidase with a broad spectrum of action, isolated from the filtrate of the fungus Tritirachium album limber.

Characteristics of Proteinase K from Tritirachium album limber:

Specificity

In addition to cleavage of peptide bonds, it is able to catalyze peptide amide hydrolysis. Proteinase K is inactivated by diisopropyl fluorophosphate (DFP) or phenyl methane sulphonyl fluoride (PMSF). Chelating agents such as citrate and EDTA have no affect on the enzyme activity.

Applications

Proteinase K is very useful in the isolation of highly native, undamaged DNAs or RNAs, since most microbial or mammalian DNases and RNases are rapidly inactivated by the enzyme, particularly in the presence of 0.5 - 1% SDS.

Characteristics of Proteinase K
Molecular Weight

28,900 daltons.

Optimal pH

7.5 - 12, using denatured hemoglobin as substrate.

Storage

PROK/PROKR: Store at 2 - 8°C; PROKS/PROKRS: Store @ -20°C

Worthington PROK/PROKR is supplied as a highly purified lyophilized powder. Code PROKS/PROKRS is a 20mg/ml solution containing 50% glycerol.  Both are tested to be free of DNase and RNase.

Stability

Although calcium ions do not affect the enzyme activity, they do contribute to its stability when present at a concentration of 1 - 5 μmoles. An interesting characteristic of proteinase K is that it retains its activity in the presence of sodium dodecyl sulphate (SDS) or urea. (0.5 - 1% SDS and 1 - 4 M urea). Raising the temperature of the reaction from 37°C to 50°C - 60°C can increase the activity several folds. A special feature of proteinase K is its ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process.

Proteinase K Products

Description
Activity
Code
Cat. #
Size
Price
Description
Proteinase K, Solution
Source:
Tritirachium album limber
A concentrated, ready to use liquid formulation. Proteinase K prepared at 20mg/ml in 10mM Tris-HCl, 1mM calcium acetate, pH 7.5 containing 50% glycerol. DNase- and RNase- free.
Store at -20°C.
Ice Pack required
Activity
≥400 u/ml
Code
PROKS
Description
Proteinase K Recombinant, Animal Free/AF
Source:
Recombinant PROK produced in yeast
Animal Free/AF, Molecular Biology Grade purified recombinant Tritiachium album Proteinase K produced in yeast. Free of DNase and RNase. Supplied as a lyophilized powder. Store at 2-8°C.
Store at 2-8°C.
Activity
>20 units per mg dry weight
Code
PROKR
LS004248
25 mg
$42.00
LS004249
100 mg
$89.00
LS004250
1 gm
$682.00
LS004252
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 20000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Proteinase K, Recombinant Solution
Source:
Tritirachium album limber
A concentrated, ready to use liquid formulation. Recombinant Proteinase K prepared at 20mg/ml in 10mM Tris-HCl, 1mM calcium acetate, pH 7.5 containing 50% glycerol. DNase and RNase free. Store at -20°C.
Store at -20°C.
Ice Pack required
Activity
>400 u/ml
Code
PROKRS
LS004254
5 ml
$135.00
LS004256
25 ml
$545.00
LS004258
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 500 x 1 ml. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.