Hydroxysteroid Dehydrogenase Product Information


Source: Pseudomonas testosteroni

I.U.B.: 1.1.1.51 and 1.1.1.50

Hydroxysteroid dehydrogenases catalyze the interconversion of hydroxyl and carboxyl groups of steroids. P.testosteroni derived hydroxysteroid dehydrogenases are of two types: 3-α-hydroxysteroid dehydrogenase (α enzyme) and 3-β-hydroxysteroid dehydrogenase (β enzyme). The α enzyme has a molecular weight of 47,000 daltons. The α enzyme oxidizes only 3-α-hydroxysteroids of the C19, C21 and C24 series. It is inhibited by heavy metals and sulfhydryl-binding agents. The β enzyme catalyzes the oxidation of 3-β-hydroxysteroids of the C19 and C21 series, 17-β-hydroxysteroids of the C18, C19 and C21 series, as well as certain 16-β-hydroxysteroids. It is inhibited by heavy metals and reducing agents. The oxidation of testosterone is inhibited by 3,17-α-estradiol and other 1,3,5-estradiene derivatives. Worthington supplies two preparations: one from the regular P. testosteroni (ATCC 11966) culture which produces both the α and the β enzymes, and a second from a mutant strain which produces almost exclusively the α enzyme. By using both, the β-hydroxysteroid can be determined by difference.

Technical Note: STDHP and STDH contain both α and β activities. STDHMP, however, contains only α activity.

Unit Definition: One Unit reduces one micromole of NAD per minute at 25°C, pH 9.0 using androsterone or testosterone as substrate.

DescriptionActivityCodeSizeCat. No.Price
Hydroxysteroid Dehydrogenase
A purified powder obtained from induced cells. Contains both alpha and beta activities.
Store at -20C.
REQUIRES SPECIAL SHIPPING: ICE PACK
≥0.03 units per mg dry weight STDH 1 gm LS004915 $73.00 Buy Now
5 gm LS004916 $292.00 Buy Now
Bulk LS004918 Inquire
Hydroxysteroid Dehydrogenase
A purified powder obtained from adapted cells of a mutant strain. Activity on androsterone only, no activity exhibited on testosterone.
Store at -20C.
REQUIRES SPECIAL SHIPPING: ICE PACK
≥0.5 units per mg dry weight STDHMP 10 un LS004908 $90.00 Buy Now
50 un LS004910 $320.00 Buy Now
Bulk LS004911 Inquire
Hydroxysteroid Dehydrogenase
A purified powder obtained from induced cells. Contains both alpha and beta activities.
Store at -20C.
REQUIRES SPECIAL SHIPPING: ICE PACK
≥0.5 units per mg dry weight STDHP Bulk LS004922 Inquire

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