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Catalase - Assay

Source:
Bovine Liver
CAS:
9001-05-2
EC:
1.11.1.6
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Catalase Assay

Method
There are numerous assays for catalase. Gregory and Fridovich (1974) report on a sensitive activity stain for catalase applicable to a polyacrylamide gel electrophoretogram, Haining and Legan (1972) describe a polarographic assay utilizable in tissue homogenates, and Kroll et al. (1989) discuss a rapid method for estimating the bacterial content of foods. The subject has been reviewed by Maehly and Chance (1954) and Chance and Maehly (1955). The assay used at Worthington follows:

 

Method: Essentially that described by Beers and Sizer (1952) in which the disappearance of peroxide is followed spectrophotometrically at 240 nm. One Unit decomposes one micromole of H2O2 per minute at 25°C and pH 7.0 under the specified conditions.

Reagents
  • 0.05 M Potassium phosphate, pH 7.0
  • 0.059 M Hydrogen peroxide (30%) in 0.05 M potassium phosphate, pH 7.0
Enzyme

Immediately prior to use dilute the enzyme in 0.05 M phosphate buffer, pH 7.0 to obtain a rate of 0.03-0.07 ΔA/min.

equation

Procedure

Adjust the spectrophotometer to 240 nm and 25°C.

Pipette into each cuvette as follows:

Reagent grade water 1.9 ml
0.059 M Hydrogen peroxide 1.0 ml

Incubate in spectrophotometer for 4-5 minutes to achieve temperature equilibration and to establish blank rate if any. Add 0.1 ml of diluted enzyme and record decrease in absorbance at 240 nm for 2-3 minutes. Calculate ΔA240/min from the initial (45 second) linear portion of the curve.

Calculation

Catalase Products

Description
Activity
Code
Cat. #
Size
Price
Description
Catalase, Suspension
Source:
Bovine Liver
A crystalline aqueous suspension of approximately 6 mg/ml containing thymol as a preservative.
Store at 2-8°C. DO NOT STORE IN PLASTIC: CONTAINS THYMOL.
…
Activity
≥20,000 units per mg protein
Code
CTR
LS001872
10 ml
$58.00
LS001873
100 ml
$425.00
LS001874
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 2000 x 1 ml. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Catalase, Filtered
Source:
Bovine Liver
Supplied as an aqueous solution of 2X crystallized catalase (Code: CTR without thymol) filtered through a 0.22 micron pore size membrane. Minimum of 30,000 units/ml; 10 ml/vial.
Store at 2-8°C. DO NOT FREEZE.
…
Activity
≥40,000 units per mg protein
Code
CTS
LS001896
10 ml
$48.00
LS001898
10x10 ml
$350.00
Description
Catalase, Lyophilized
Source:
Bovine Liver
A partially purified, lyophilized powder.
Store at -20°C. PROTECT FROM MOISTURE.
…
Activity
≥3,000 units per mg protein
Code
CTL
LS001847
2 gm
$80.00
LS001849
10 gm
$334.00
LS001851
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 200 x 1 gm. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.