I purchased phosphodiesterase I as a powder. I need to weight very small amounts of powder in each experiment and I prefer to dissolve it in a buffer and keep it like that. Is it O.K to do so? and if so, in what buffer?
Our QC department reconstitutes the 100 unit vial of phosphodiesterase I (code VPH) with 2ml of 0.11M Tris pH 8.9 containing 0.11M NaCl and 15 mM MgCl, aliquots (to avoid repeated freeze/thaw cycles) and freezes at -20°C. Under these conditions the frozen aliquots are stable for several months