What types of media work best with Collagenase I? A colleague of mine uses DMEM but we always use PBS with either 2% FBS or 0.5% BSA. Do you have any suggestions of what might be the best media? We are using it for mouse bone marrow isolation.
Collagenase works well in most balanced salt solutions (ie. Hanks, Earles, PBS, DMEM). The choice is usually more application or tissue specific. We are not working with murine bone marrow here, so I hesitate to choose one over the other. Suggestions are usually based on references. You can search our Tissue Dissociation Guide at www.tissuedissociation.com for references by tissue type.