Previously I've used Dispase II at around 2.5 mg/ml at 37C for 2hr or overnight at 4C to dissociate epidermis from dermis in skin biopsies. What concentration of your neutral protease do you recommend for this procedure?
Dispase II usually refers to a partially purified grade of dispase
similiar to Worthington's NPRO2. Historically most lots of NPRO2
average around 0.4 u/mg so 1u/ml of our neutral protease should be
similar to 2.5 mg Dispase II. Neutral protease working concentrations
are usually in the 0.5-2.0 u/ml range.