How can I convert USP/NF units/mg dw of hyaluronidase into U/ml?
Worthington supplies hyaluronidase as a lyophilized powder reporting the u/mg dry weight on the Certificate of Analysis. You can resuspend hyaluronidase to the desired u/ml using this information.
As an example, the current lot of hyaluronidase was released at 525 u/mg dw, if you need a 500 u/ml solution you would dilute 5 mg with 5.25 ml to obtain the 500 u/ml. Calculation 5mg*525u/mg=2625 units/5.25 ml=500 u/ml