Can I use the Neonatal Cardiomyocyte Isolation System with mice?
The Neonatal Cardiomyocyte Isolation System is specifically packaged and use tested on neonatal rats.
A few researchers have used the system on neonatal mice, with some modifications. I have copied some references below for neonatal mice. It seems neonatal mouse hearts may require higher enzyme concentrations and/or longer digestion times than the rats. Increasing the collagenase digestion time to 45 min is a good place to start your optimization. Also one researcher has informed me he increased the trypsin concentration to 100 ug/ml by cutting final volumes in half.
The protocol for the NCIS kit can viewed from the following link-