I bought Deoxyribonuclease I (DCLS) from your company. How can I make the stock solution? Can I disslove the powder in distilled water?
Depending on the application DNase I can be dissolved in water or numerous buffers. Once reconstituted the material should be aliquoted and frozen at -20°C or -80°C. Avoid repeat freeze/ thaw cycles. For long term storage we dissolve Dnase I in 5mM acetate buffer pH 4.5 containing 1mM calcium. It is best to freeze stocks near 1mg/ml as very dilute solutions will not be as stable.