Can the Neonatal Cardiomyocyte Isolation System (NCIS Kit) be used on adults?
The NCIS kit is specifically packaged for five separate tissue dissociations, each containing up to twelve neonatal rat hearts. The NCIS protocol is available online at www.worthington-biochem.com/NCIS/default.html. Adult rat cardiomyocytes usually require perfusion ie. Langendorf Perfusion System and Collagenase Type II.