What is an acceptable enzyme diluent for Neutral Protease/Dispase (code NPRO/NPRO2)?
Neutral protease is usually dissolved in neutral balanced salt solution of choice (ie PBS, Hanks, Earles etc). The composition of several balanced salt solutions are posted in our Tissue Dissociation Guide at http://www.worthington-biochem.com/tissuedissociation/working.html
Usual working solutions for Neutral Protease are 0.5-3.0 u/ml but this varies widely and is dependent on application, diluent, incubation time and temperature. We generally recommend diluting the enzyme as needed as the lyophilized powder is stable at 2-8C. Reconstituted enzymes should not be stored at 2 - 8°C. If necessary they can be aliquoted and frozen at -20°C. Avoid repeated freeze-thaw cycles.