Worthington Biochemical FAQ

What is an acceptable enzyme diluent for Elastase (ES)?

Elastase has poor solubility at neutral pH and concentrations greater than 0.25%. It is helpful to make primary solutions in KCl or alkaline buffers before diluting into reaction mixtures or media, compensating for ionic strength or pH changes. Elastase is stable at pH 4.0-10.4, optimum pH 8.5. Elastase is unstable at pH below 3.5. Elastase should be sterile filtered through a 0.22um pore size membrane before use. DO NOT FREEZE.