Technical Support FAQ - Most recently posted questions:

I ordered Collagenase IV from your company (100mg). Some of the protocols call for 2mg/ml concentration and others call for 0.2% solution. How does one prepare a 0.2% solution? Thanks for your time!

Thu, 07 Aug 2008 13:38:56 GMT

A 0.2% solution is equivalent to 2 mg/ml. Just remember a 1% solution refers to 1 gram per 100 ml or 10 mg/ml.

I bought Deoxyribonuclease I (DCLS) from your company. How i can make the stock solution? Can I disslove the powder in distilled water?

Wed, 30 Jul 2008 20:47:20 GMT

Depending on the application DNase I can be dissolved in water or numerous buffers. Once reconstituted the material should be aliquoted and frozen at -20C or -80C. Avoid repeat freeze/ thaw cycles. For long term storage we dissolve Dnase I in 5mM acetate buffer pH 4.5 containing 1mM calcium. It is best to freeze stocks near 1mg/ml as very dilute solutions will not be as stable.

I got a phosphodiesterase I as a powder. I need to weight very small amounts of powder in each experiment and I prefer to dissolve it in a buffer and keep it like that. Is it O.K to do so? and if so, in what buffer?

Tue, 03 Jun 2008 14:51:40 GMT

Our QC department reconstitutes the 100 unit vial of phosphodiesterase I (code VPH) with 2ml of 0.11M Tris pH 8.9 containing 0.11M NaCl and 15 mM MgCl, aliquots (to avoid repeated freeze/thaw cycles) and freezes at -20C. Under these conditions the frozen aliquots are stable for several months

Are your sterile filtered collagenase preps tested for LPS?

Tue, 03 Jun 2008 14:45:58 GMT

We are not currently testing our collagenase products for endotoxin or LPS. The filtered collagense is a typical batch of bacterial collagenase that is resuspended and 0.22 micron filtered then lyophilized in autoclaved vials. Enzymatic activity is rechecked and colony counts are preformed with <1 cfu/ml acceptance criteria.

How to the different types of collagenase offered by Worthington compare to collagenase from other suppliers?

Mon, 14 Jan 2008 15:23:47 GMT

Worthington Collagenase Cross Reference Chart

Worthington CLS-1	Worthington CLS-2	Worthington CLS-3	Worthington CLS-4
Invitrogen/Gibco I	Invitrogen/Gibco II	Invitrogen/Gibco III	Invitrogen/Gibco IV
Roche Collagenase A	Roche Collagenase B		Roche Collagenase D
Sigma Type I
USB type I

Technical Support FAQ - Most recently posted questions:

I ordered Collagenase IV from your company (100mg). Some of the protocols call for 2mg/ml concentration and others call for 0.2% solution. How does one prepare a 0.2% solution? Thanks for your time!

I bought Deoxyribonuclease I (DCLS) from your company. How i can make the stock solution? Can I disslove the powder in distilled water?

I got a phosphodiesterase I as a powder. I need to weight very small amounts of powder in each experiment and I prefer to dissolve it in a buffer and keep it like that. Is it O.K to do so? and if so, in what buffer?

Are your sterile filtered collagenase preps tested for LPS?

How to the different types of collagenase offered by Worthington compare to collagenase from other suppliers?

Worthington Collagenase Cross Reference Chart

Worthington CLS-1

Worthington CLS-2

Worthington CLS-3

Worthington CLS-4