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Questions in Tissue Dissociation
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- We ordered hyaluronidase from worthington cat no LS005474. the activity says that it is >/3000 USP/NF units per mg dry weight. Can you please convert it to mg. thanks
(16228 views) - Which type of collagenase is present in your purified collagenase, CLSPA?
(15825 views) - I ordered Collagenase IV from your company (100mg). Some of the protocols call for 2mg/ml concentration and others call for 0.2% solution. How does one prepare a 0.2% solution?
Thanks for your time!
(65289 views) - Are your sterile filtered collagenase preps tested for LPS?
(52617 views) - How do the different types of collagenase offered by Worthington compare to collagenase from other suppliers?
(100409 views) - How can I convert USP/NF units/mg dw of hyaluronidase into U/ml?
(33208 views) - Can I use the Neonatal Cardiomyocyte Isolation System with mice?
(30761 views) - Previously I've used Dispase II at around 2.5 mg/ml at 37C for 2hr or overnight at 4C to dissociate epidermis from dermis in skin biopsies. What concentration of your neutral protease do you recommend for this procedure?
(99902 views) - I have a question relating to your "Neonatal Cardiomyocyte Isolation System" protocol. Is there any serum added to your L-15 media provided in the kit or do I need to add some separately? If the serum is already present, would it be possible to culture the cells in the absence of any serum?
(98382 views) - What is the expiration time for the neonatal cardiomyocyte isolation system?
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