Colon - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Human Human Colonic epithelial Collagenase: 150 u/ml
Neutral Protease: 0.04 mg/ml
 Basal X media Roig, A., Eskiocak, U., Hight, S., Kim, S., Delgado, O., Souza, R., Spechler, S., Wright, W. and Shay, J.: Immortalized Epithelial Cells Derived from Human Colon Biopsies Express Stem Cell Markers and Differentiate In Vitro., Gastroenterol 138, 1012, 2010
Human Human Colorectal cancer Collagenase Type 4: 1%
Deoxyribonuclease I: 0.2%
 HBSS Zhou, J., Belov, L., Huang, P., Shin, J., Solomon, M., Chapuis, P., Bokey, L., Chan, C., Clarke, C., Clarke, S. and Christopherson, R.: Surface Antigen Profiling of Colorectal Cancer Using Antibody Microarrays With Fluorescence Multiplexing., J Immunol Methods 355, 40, 2010
Human Human Colonic epithelial Collagenase Type 4: 0.1%
 not listed Huang, E., Hynes, M., Zhang, T., Ginestier, C., Dontu, G., Appelman, H., Fields, J., Wicha, M. and Boman, B.: Aldehyde Dehydrogenase 1 is a Marker for Normal and Malignant Human Colonic Stem Cells (SC) and Tracks SC Overpopulation During Colon Tumorigenesis., Cancer Res 69, 3382-9, 2009
Human Human Cancer stem cell Collagenase Type 3: 200 u/ml
Deoxyribonuclease I: 100 u/ml
 RPMI-1640 Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F: Phenotypic characterization of human colorectal cancer stem cells, Proc Natl Acad Sci U S A 104, 10158-63, 2007
Human Human Colonic epithelial Collagenase:
Neutral Protease: 0.3%
Deoxyribonuclease I: 0.05%
 RPMI 1640 Fukushima, K. and Fiocchi, C.: Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients.,Am J Physiol Gastrointest Liver Physiol Vol. 286,, , G804-13, 2004
Human Human Colonic endothelial cells Collagenase Type 2: 0.25%
 HBSS/5%FBS Wang D., Lehman R., Donner D., Matli M., Warren R., and Welton M.: Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells, Am J Physiol/Gastro 282, G1088, 2002
Human Human Colonocytes Collagenase:
DMEM/F12 Emenaker N, Calaf G, Cox D, Basson M and Qureshi N: Short chain fatty acids differentially modulate cellular phenotype and c-myc protein levels in primary human nonmalignant and malignant colonocytes, J Nutr 46, 96-105, 2001
Human Human Epithelial and mucosal lymphocytes Neutral Protease: 0.1%
CLSPA: 0.02%
Deoxyribonuclease I: 0.01%
 RPMI 1640 Hisamatsu, T., Watanabe, M., Ogata, H., Ezaki, T., Hozawa, S., Ishii, H., Kanai, T. and Hibi, T.: Interferon-Inducible Gene Family 1-8U Expression in Colitis-Associated Colon Cancer and Severely Inflamed Mucosa in Ulcerative Colitis., Cancer Res 59, 5927-31, 1999
Human Human Colonic epithelial Neutral Protease: 1.2 u/ml
Collagenase Type 4: 50 u/ml
 HBSS Gibson, P., Rosella, O., Wilson, A., Mariadason, J., Rickard, K., Byron, K. and Barkla, D.: Colonic Epithelial Cell Activation and the Paradoxical Effects of Butyrate., Carcinogenesis 20, 539, 1999
Human Human Lamina propria lymphocytes Collagenase: 25 u/ml
 HBSS Ueyama H, Kiyohara T, Sawada N, Isozaki K, Kitamura S, Kondo S, Miyagawa J, Kanayama S, Shinomura Y, Ishikawa H, Ohtani T, Nezu R, Nagata S, Matsuzawa Y: High Fas ligand expression on lymphocytes in lesions of ulcerative colitis, Gut 43, 48-55, 1998
Mouse Mouse Lamina propria Collagenase Type 2: 0.1%
Neutral Protease: 0.1%
Deoxyribonuclease I: 0.004%
 RPMI 1640 Atarashi, K., Nishimura, J., Shima, T., Umesaki, Y., Yamamoto, M., Onoue, M., Yagita, H., Ishii, N., Evans, R., Honda, K. and Takeda, K.: ATP Drives Lamina Propria T(H)17 Cell Differentiation., Nature 455, 808, 2008
Mouse Mouse, 6-8 week Cancer stem cell Collagenase Type 3: 200 u/ml
Deoxyribonuclease I: 100 u/ml
 RPMI-1640 Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F: Phenotypic characterization of human colorectal cancer stem cells, Proc Natl Acad Sci U S A 104, 10158-63, 2007
Mouse Mouse Lamina propria mononuclear cells Collagenase: 0.05%
Deoxyribonuclease I: 0.05%
Neutral Protease: 0.3%
 HBSS Weigmann Benno, Tubbe Ingrid, Seidel Daniel, Nicolaev Alex, Becker Christoph, Neurath Markus F: Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue, Nat Protoc 2, 2307-11, 2007
Mouse Mouse Dentritic Collagenase: 300 u/ml
Deoxyribonuclease I: 0.002%
 RPMI 1640 Abe, K., Nguyen, K., Fine, S., Mo, J., Shen, C., Shenouda, S., Corr, M., Jung, S., Lee, J., Eckmann, L. and Raz, E.: Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells., Proc Natl Acad Sci U S A 104, 17022, 2007
Mouse Mouse Lympocytes Collagenase/Dispase: 100 u/ml
 RPMI 1640 Annacker O, Coombes JL, Malmstrom V, Uhlig HH, Bourne T, Johansson-Lindbom B, Agace WW, Parker CM, Powrie F.: Essential role for CD103 in the T cell-mediated regulation of experimental colitis, J Exp Med 202, 1051, 2005
Mouse Mouse, 6-8 week Lamina propria lymphocytes Collagenase Type 1: 0.2%
Deoxyribonuclease I: 0.01%
 HBSS Totsuka T, Kanai T, Uraushihara K, Iiyama R, Yamazaki M, Akiba H, Yagita H, Okumura K, Watanabe M: Therapeutic effect of anti-OX40L and anti-TNF-alpha MAbs in a murine model of chronic colitis, Am J Physiol/Gastro 284, G595-603, 2003
Mouse Mouse, 6-8 week Lamina propria mononuclear cells Collagenase Type 2: 0.015%
Deoxyribonuclease I: 0.01%
 RPMI Wirtz S., Becker C., Blumberg R., Galle P., and Neurath M.: Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA, J Immunol 168(1), 411, 2002