Colon - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Guinea-Pig Guinea-pig, adult Enterochromaffin Trypsin: 0.05%
Collagenase Type 1: 0.1%
DMEM Raghupathi, R., Duffield, M., Zelkas, L., Meedeniya, A., Brookes, S., Sia, T., Wattchow, D., Spencer, N. and Keating, D.: Identification of Unique Release Kinetics of Serotonin From Guinea-pig and Human Enterochromaffin Cells., J Physiol 591, 5959-75, 2013
Guinea-Pig Guinea-Pig, male, 200-250g Myenteric ganglia Collagenase Type 4: 0.2%
Protease: 0.1%
Kreb's solution Kang, M., Maguma, H., Smith, T., Ross, G., Dewey, W. and Akbarali, H.: The Role of β-arrestin2 in the Mechanism of Morphine Tolerance in the Mouse and Guinea Pig Gastrointestinal Tract., J Pharmacol Exp Ther 340, 567-76, 2012
Human Human Colon enteric neural Neutral Protease: 0.5%
Collagenase animal free: 0.1%
Hyaluronidase: 0.1%
Deoxyribonuclease I: 0.01%
Ham's F-12 Rollo, B., Zhang, D., Stamp, L., Menheniott, T., Stathopoulos, L., Denham, M., Dottori, M., King, S., Hutson, J. and Newgreen, D.: Enteric Neural Cells from Hirschsprung Disease Patients Form Ganglia in Autologous Aneuronal Colon, Cell Mol Gastroenterol Hepatol 2, 92, 2016
Human Human Enterochromaffin Trypsin: 0.05%
Collagenase Type 1: 0.1%
DMEM Raghupathi, R., Duffield, M., Zelkas, L., Meedeniya, A., Brookes, S., Sia, T., Wattchow, D., Spencer, N. and Keating, D.: Identification of Unique Release Kinetics of Serotonin From Guinea-pig and Human Enterochromaffin Cells., J Physiol 591, 5959-75, 2013
Human Human Colonic epithelial Collagenase: 150 u/ml
Neutral Protease: 0.04 mg/ml
Basal X media Roig, A., Eskiocak, U., Hight, S., Kim, S., Delgado, O., Souza, R., Spechler, S., Wright, W. and Shay, J.: Immortalized Epithelial Cells Derived from Human Colon Biopsies Express Stem Cell Markers and Differentiate In Vitro., Gastroenterol 138, 1012, 2010
Human Human Colorectal cancer Collagenase Type 4: 1%
Deoxyribonuclease I: 0.2%
HBSS Zhou, J., Belov, L., Huang, P., Shin, J., Solomon, M., Chapuis, P., Bokey, L., Chan, C., Clarke, C., Clarke, S. and Christopherson, R.: Surface Antigen Profiling of Colorectal Cancer Using Antibody Microarrays With Fluorescence Multiplexing., J Immunol Methods 355, 40, 2010
Human Human Colonic epithelial Collagenase Type 4: 0.1%
not listed Huang, E., Hynes, M., Zhang, T., Ginestier, C., Dontu, G., Appelman, H., Fields, J., Wicha, M. and Boman, B.: Aldehyde Dehydrogenase 1 is a Marker for Normal and Malignant Human Colonic Stem Cells (SC) and Tracks SC Overpopulation During Colon Tumorigenesis., Cancer Res 69, 3382-9, 2009
Human Human Colon cancer Collagenase Type 1: 300 u/ml
Hyaluronidase: 100 u/ml
DMEM/F12 Varnat, F., Duquet, A., Malerba, M., Zbinden, M., Mas, C., Gervaz, P. and Ruiz i Altaba, A.: Human Colon Cancer Epithelial Cells Harbour Active HEDGEHOG-GLI Signalling that is Essential for Tumour Growth, Recurrence, Metastasis and Stem Cell Survival and Expansion., EMBO Mol Med 1, 338-51, 2009
Human Human Cancer stem cell Collagenase Type 3: 200 u/ml
Deoxyribonuclease I: 100 u/ml
RPMI-1640 Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F: Phenotypic characterization of human colorectal cancer stem cells, Proc Natl Acad Sci U S A 104, 10158-63, 2007
Human Human Colonic epithelial Collagenase:
Neutral Protease: 0.3%
Deoxyribonuclease I: 0.05%
RPMI 1640 Fukushima, K. and Fiocchi, C.: Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients., Am J Physiol Gastrointest Liver Physiol 286, G804-13, 2004
Human Human Colonic endothelial cells Collagenase Type 2: 0.25%
HBSS/5%FBS Wang D., Lehman R., Donner D., Matli M., Warren R., and Welton M.: Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells, Am J Physiol/Gastro 282, G1088, 2002
Human Human Colonocytes Collagenase:
DMEM/F12 Emenaker N, Calaf G, Cox D, Basson M and Qureshi N: Short chain fatty acids differentially modulate cellular phenotype and c-myc protein levels in primary human nonmalignant and malignant colonocytes, J Nutr 46, 96-105, 2001
Human Human Epithelial and mucosal lymphocytes Neutral Protease: 0.1%
CLSPA: 0.02%
Deoxyribonuclease I: 0.01%
RPMI 1640 Hisamatsu, T., Watanabe, M., Ogata, H., Ezaki, T., Hozawa, S., Ishii, H., Kanai, T. and Hibi, T.: Interferon-Inducible Gene Family 1-8U Expression in Colitis-Associated Colon Cancer and Severely Inflamed Mucosa in Ulcerative Colitis., Cancer Res 59, 5927-31, 1999
Human Human Colonic epithelial Neutral Protease: 1.2 u/ml
Collagenase Type 4: 50 u/ml
HBSS Gibson, P., Rosella, O., Wilson, A., Mariadason, J., Rickard, K., Byron, K. and Barkla, D.: Colonic Epithelial Cell Activation and the Paradoxical Effects of Butyrate., Carcinogenesis 20, 539, 1999
Human Human Lamina propria lymphocytes Collagenase: 25 u/ml
HBSS Ueyama H, Kiyohara T, Sawada N, Isozaki K, Kitamura S, Kondo S, Miyagawa J, Kanayama S, Shinomura Y, Ishikawa H, Ohtani T, Nezu R, Nagata S, Matsuzawa Y: High Fas ligand expression on lymphocytes in lesions of ulcerative colitis, Gut 43, 48-55, 1998
Mouse Mouse Lamina propria Collagenase Type 2: 0.1%
Neutral Protease: 0.1%
Deoxyribonuclease I: 0.004%
RPMI 1640 Atarashi, K., Nishimura, J., Shima, T., Umesaki, Y., Yamamoto, M., Onoue, M., Yagita, H., Ishii, N., Evans, R., Honda, K. and Takeda, K.: ATP Drives Lamina Propria T(H)17 Cell Differentiation., Nature 455, 808, 2008
Mouse Mouse, 6-8 week Cancer stem cell Collagenase Type 3: 200 u/ml
Deoxyribonuclease I: 100 u/ml
RPMI-1640 Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F: Phenotypic characterization of human colorectal cancer stem cells, Proc Natl Acad Sci U S A 104, 10158-63, 2007
Mouse Mouse Lamina propria mononuclear cells Collagenase: 0.05%
Deoxyribonuclease I: 0.05%
Neutral Protease: 0.3%
HBSS Weigmann Benno, Tubbe Ingrid, Seidel Daniel, Nicolaev Alex, Becker Christoph, Neurath Markus F: Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue, Nat Protoc 2, 2307-11, 2007
Mouse Mouse Dentritic Collagenase: 300 u/ml
Deoxyribonuclease I: 0.002%
RPMI 1640 Abe, K., Nguyen, K., Fine, S., Mo, J., Shen, C., Shenouda, S., Corr, M., Jung, S., Lee, J., Eckmann, L. and Raz, E.: Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells., Proc Natl Acad Sci U S A 104, 17022, 2007
Mouse Mouse Lympocytes Collagenase/Dispase: 100 u/ml
RPMI 1640 Annacker O, Coombes JL, Malmstrom V, Uhlig HH, Bourne T, Johansson-Lindbom B, Agace WW, Parker CM, Powrie F.: Essential role for CD103 in the T cell-mediated regulation of experimental colitis, J Exp Med 202, 1051, 2005
Mouse Mouse, 6-8 week Lamina propria lymphocytes Collagenase Type 1: 0.2%
Deoxyribonuclease I: 0.01%
HBSS Totsuka T, Kanai T, Uraushihara K, Iiyama R, Yamazaki M, Akiba H, Yagita H, Okumura K, Watanabe M: Therapeutic effect of anti-OX40L and anti-TNF-alpha MAbs in a murine model of chronic colitis, Am J Physiol/Gastro 284, G595-603, 2003
Mouse Mouse, 6-8 week Lamina propria mononuclear cells Collagenase Type 2: 0.015%
Deoxyribonuclease I: 0.01%
RPMI Wirtz S., Becker C., Blumberg R., Galle P., and Neurath M.: Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA, J Immunol 168(1), 411, 2002
Rat Rat, adult Colon smooth muscle Soybean Trypsin Inhibitor: 0.01%
Collagenase Type 2: 0.1%
DMEM Somara, S., Bashllari, D., Gilmont, R. and Bitar, K.: Real-Time Dynamic Movement of Caveolin-1 During Smooth Muscle Contraction of Human Colon and Aged Rat Colon Transfected with Caveolin-1 cDNA., Am J Physiol Gastrointest Liver Physiol 300, G1022, 2011