Endothelial - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Bovine umbilical cord (BUVEC) Collagenase: 0.1%
Dulbecco's/Ham F-12 Ricken Albert M, Traenkner Anja, Merkwitz Claudia, Hummitzsch Katja, Grosche Jens, Spanel-Borowski Katharina: The short prolactin receptor predominates in endothelial cells of micro- and macrovascular origin, J Vasc Res 44, 19-30, 2007
Bovine Bovine Pulmonary artery endothelial and smooth muscle cells Collagenase: 0.04-0.05%
Soybean Trypsin Inhibitor: 0.04%
RPMI-1640 Yu, M., McAndrew, R., Al-Saghir, R., Maier, K., Medhora, M., Roman, R. and Jacobs, E.: Nitric Oxide Contributes to 20-HETE-Induced Relaxation of Pulmonary Arteries., J Appl Physiol 93, 1391, 2002
Bovine Bovine, (Bos taurus), calf Endothelial
Collagenase: 1000 u/ml
PBS, CMF Del Vecchio, P.J., Siflinger-Birnboim, A., Belloni, P.N., Holleron, L.A., Lum, H., and Malik, A.B.: Culture and Characterization of Pulmonary Microvascular Endothelial Cell, In Vitro Cell Dev Biol 28A, 711, 1992
Bovine Calf Endothelial Trypsin: 0.25%
HEPES Vender, R.: Role of Endothelial Cells in the Proliferative Response of Cultured Pulmonary Vascular Smooth Muscle Cells to Reduced Oxygen Tension, In Vitro Cell Dev Biol 28A, 403, 1992
Bovine Bovine, adult Cerebral artery
Collagenase: 0.2%
HBSS Machi, T., Kassell, N.F., and Scheld, W.M.: Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries, In Vitro Cell Dev Biol 26, 291, 1990
Bovine Bovine Endothelial
Trypsin: 0.05%
Krebs Ringer solution DeNucci, G., Gryglewski, R.J., Warner, T.D., and Vane, J.R.: Receptor-Mediated Release of Endothelium-Derived Relaxing Factor and Prostacyclin From Bovine Aortic Endothelial Cells Is Coupled, Proc Natl Acad Sci U S A 85, 2334, 1988
Bovine Bovine Endothelial, pulmonary artery Collagenase: 0.1%
CMF-Dulbecco's PBS Martin, T.: Formation of Diacylglycerol by a Phospholipase D-phosphatidate Phosphatase Pathway Specific for Phosphatidylcholine in Endothelial Cells, Biochim Biophys Acta 962, 282, 1988
Bovine Bovine Endothelial
Collagenase Type 2: 0.1%
PBS Carson, M.P. and Haudenschild, C.C.: Microvascular Endothelium and Pericytes: High Yield, Low Passage Cultures, In Vitro Cell Dev Biol 22, 344, 1986
Bovine Bovine Endothelial
Collagenase Type 2: 0.1%
DMEM Kinsella, Michael G. and Wight, Thomas N.: Modulation of Sulfated Proteoglycan Synthesis by Bovine Aortic Endothelial Cells During Migration, J Cell Biol 102, 679, 1986
Bovine Bovine Endothelial Collagenase: 0.5%
DMEM/Ham's F-12 Gospodarowicz, D., Massoglia, S., Cheng, J., and Fujii, D.: Effect of Fibroblast Growth Factor and Lipoproteins on the Proliferation of Endothelial Cells Derived From Bovine Adrenal Cortex, Brain Cortex, and Corpus Luteum Capillaries, J Cell Physiol 127, 121, 1986
Bovine Bovine Endothelial
Brain arteries
Collagenase Type 2: 0.2%
Dulbecco's PBS Goetz, I.., Warren, J., Estrada, C., Roberts, E., and Krause, D.: Long-Term Cultivation of Arterial and Capillary Endothelium From Adult Bovine Brain, In Vitro Cell Dev Biol 21, 172, 1985
Bovine Calf Endothelial
Smooth muscle
Collagenase: 0.75%
DMEM Voyta, J., Via, D., Butterfield, C., and Zetter, B.: Identification and Isolation of Endothelial Cells Based on Their Increased Uptake of Acetylated-Low Density Lipoprotein, J Cell Biol 99, 2034, 1984
Bovine Bovine Endothelial
Trypsin: 0.2%
PBS: DMEM Scott, D., Murray, J., and Barnes, M.: Investigation of the Attachment of Bovine Corneal Endothelial Cells, Exp Cell Res 144, 472, 1983
Bovine Bovine, 2-3 weeks Endothelial
Pulmonary artery
Collagenase Type 1: 0.2%
RPMI 1640 w/1% Fetal Bovine Serum Lee, S., Douglas, W., Deneke, S., and Fanburg, B.: Ultrastructural Changes in Bovine Pulmonary Artery Endothelial Cells Exposed to 80% O2 In Vitro, In Vitro 19, 714, 1983
Bovine Bovine Endothelial, Corneal Trypsin: 0.05%
0.01M Phosphate buffer with 0.02% EDTA
0.9% NaCl (See Reference)
Robinson, J. and Gospodarowicz, D.: Glycosaminoglycans Synthesized by Cultured Bovine Corneal Endothelial Cells, J Cell Physiol 117, 368, 1983
Bovine Bovine Endothelial
Subclavian vein
Collagenase Type 1: 0.10%
PBS Olander, J., Marasa, J., Kimes, R., Johnston, G., and Feder, J.: An assay measuring the stimulation of several types of bovine endothelial cells by growth factor(s) derived from cultured human tumor cells, In Vitro 18, 99, 1982
Bovine Bovine Aortic
Pulmonary artery
Collagenase Type 2: 0.10%
PBS Makarski, J. S.: Stimulation of cyclic AMP production by vasoactive agents in cultured bovine aortic and pulmonary artery endothelial cells, In Vitro 17, 450, 1981
Bovine Calf, fetal, 4-9 months Endothelial Collagenase Type 1: 0.25%
PBS Rosen, E., Mueller, S., Noveral, J., and Levine, E.: Proliferative Characteristics of Clonal Endothelial Cell Strains, J Cell Physiol 107, 123, 1981
Bovine Bovine, adult Aorta Collagenase Type 1: 125 u/ml
Dulbecco's PBS with calcium and magnesium Cotta-Pereira, G., Sage, H., Bornstein, P., Ross, R., and Schwartz, S.: Studies of Morphologically Atypical ("Sprouting") Cultures of Bovine Aortic Endothelial Cells. Growth Characteristics and Connective Tissue Protein Synthesis, J Cell Physiol 102, 183, 1980
Bovine Bovine, young Pulmonary artery Collagenase: 0.1%
Medium 199 Ryan, U., Mortara, M., and Whitaker, C.: Methods for Microcarrier Culture of Bovine Pulmonary Artery Endothelial Cells Avoiding the Use of Enzymes, Tissue Cell 12, 619, 1980
Bovine Bovine Foreskin Collagenase: 0.5%
Dulbecco's MEM w/10% calf serum Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76, 5217, 1979
Bovine Bovine Saphenous Vein
Collagenase: 0.01%
PBS Eskin, S., Sybers, H., Trevino, L., Lie, J., and Chimoskey, J.: Comparison of Tissue-Cultured Bovine Endothelial Cells from Aorta and Saphenous Vein, In Vitro 14, 903, 1978
Bovine Bovine Endothelial
Thoracic aorta
Saphenous veins
Collagenase Type 2: 0.1%
PBS Schwartz, S.M.: Selection and Characterization of Bovine Aortic Endothelial Cells, In Vitro 14, 966, 1978
Bovine Bovine Pulmonary artery Collagenase Type 2: 0.25%
Puck's solution Ryan, U.S., Clements, E., Habliston, D., and Ryan, J.W.: Isolation And Culture of Pulmonary Artery Endothelial Cells, Tissue Cell 10, 535, 1978
Bovine Calf Endothelial Collagenase Type 2: 0.1%
DMEM Howard, B., Macarak, E., Gunson, D., and Kefalides, N.: Characterization of the Collagen Synthesized by Endothelial Cells in Culture, Proc Natl Acad Sci U S A 73 (7), 2361, 1976
Canine Dog
(also human)
Microvessels Collagenase Type 4: 0.1%
DMEM Gerhart, D. Z., Broderius, M. A., and Drewes, L. R.: Cutlured Human and Canine Endothelial Cells from Brain Microvessels, Brain Res Bull 21, 785, 1988
Canine Dog, mongrel, adult Endothelium
Jugular vein
Trypsin: 0.1%
Earle's PBS, CMF Ford, J., Burkel, W., and Kahn, R.: Isolation of Adult Canine Venous Endothelium for Tissue Culture, In Vitro 17, 44, 1981
Guinea-Pig Guinea pig, female, 300-350g Coronary endothelial Collagenase Type 2: 0.1%
see reference Buxton I L, Kaiser R A, Oxhorn B C, Cheek D J: Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium, Am J Physiol Heart Circ Physiol 281, H1657-66, 2001
Human Human Vascular endothelial Collagenase Type 1: 0.1%
HBSS Ganguly, A., Zhang, H., Sharma, R., Parsons, S. and Patel, K.: Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions., J Vis Exp 66, e4032, 2012
Human Human, 18-68 yr Corneal endothelial Collagenase: 0.2%
Neutral Protease: 1.0%
DMEM/F12 Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G: A novel method of isolation, preservation, and expansion of human corneal endothelial cells, Inv Ophthal Visual Sci 48, 614-20, 2007
Human Human Endothelial and vascular smooth muscle Collagenase Type 1: 0.2%
HBSS Moss, S., Bates, M., Parrino, P. and Woods, TC.: Isolation of Endothelial Cells and Vascular Smooth Muscle Cells from Internal Mammary Artery Tissue., Ochsner J 7, 133, 2007
Human Human HUVEC Collagenase Type 1: 0.1%
HBSS Davis, J., Crampton, S. and Hughes. C.: Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)., J Vis Exp 3, 183, 2007
Human Human HUVEC Collagenase Type 4: 0.1%
RPMI 1640 Silva AP, Kaufmann JE, Vivancos C, Fakan S, Cavadas C, Shaw P, Brunner HR, Vischer U, and Grouzmann E.: Neuropeptide Y expression, localization and cellular transducing effects in HUVEC, Biol Cell 97(6), 457, 2005
Human Human Endothelial Collagenase Type 2: 0.1%
DMEM Patel, V., Logan, A., Watkinson, J., Uz-Zaman, S., Sheppard, M., Ramsden, J. and Eggo, M.: Isolation and Characterization of Human Thyroid Endothelial Cells., Am J Physiol Endocrinol Metab 284, E168, 2003
Human Human Esophageal microvascular endothelial Collagenase Type 2: 0.2%
MCDB-131 Rafiee, P., Ogawa, H., Heidemann, J., Li, M., Aslam, M., Lamirand, T., Fisher, P., Graewin, S., Dwinell, M., Johnson, C., Shaker, R. and Binion, D.: Isolation and Characterization of Human Esophageal Microvascular Endothelial Cells: Mechanisms of Inflammatory Activation., Am J Physiol Gastrointest Liver Physiol 285, G1277, 2003
Human Human Colonic endothelial cells Collagenase Type 2: 0.25%
HBSS/5%FBS Wang D., Lehman R., Donner D., Matli M., Warren R., and Welton M.: Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells, Am J Physiol/Gastro 282, G1088, 2002
Human Human Pulmonary vascular endothelial cells Neutral Protease: 1.18 u/ml
Elastase: 10 u/ml
M199 Muller AM, Hermanns MI, Skrzynski C, Nesslinger M, Muller KM, and Kirkpatrick CJ.: Expression of the endothelial markers PECAM-1, vWf, and CD34 in vivo and in vitro, Exp Mol Pathol 72, 221, 2002
Human Human HUVEC Collagenase Type 2: 0.1%
PBS Takano Manabu, Meneshian Avedis, Sheikh Emran, Yamakawa Yasuhiko, Wilkins Kirsten Bass, Hopkins Elise A, Bulkley Gregory B: Rapid upregulation of endothelial P-selectin expression via reactive oxygen species generation, Am J Physiol Heart Circ Physiol 283, H2054-61, 2002
Human Human Endothelial Trypsin: 2%
PBS Goolcharran, C., Cleland, J., Keck, R., Jones, A., and Borchardt, R.: Comparsion of the Rates of Deamidation, Diketopiperazine Formation and Oxidation in Recombinant Human Vascular Endothelial Growth Factor and Model Peptides, AAPS PharmSci 2 (1), 5, 2000
Human Human HUVEC, porcine pulmonary arterial endothelial cells Collagenase Type 2: 0.2%
DMEM Kwak HJ, Lee SJ, Lee YH, Ryu CH, Koh KN, Choi HY, and Koh GY.: Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells, Circulation 101(19), 2317, 2000
Human Human Vascular endothelial cells Collagenase: 0.1%
DMEM Schonbeck U, Sukhova GK, Graber P, Coulter S, Libby P: Augmented expression of cyclooxygenase-2 in human atherosclerotic lesions, Am J Pathol 155, 1281-91, 1999
Human Human Hepatic endothelial cells Collagenase: 0.2%
DMEM Sanyal AJ, and Mirshahi F.: A simplified method for the isolation and culture of endothelial cells from pseudointima of transjugular intrahepatic portasystemic shunts, Lab Invest 78(11), 1469, 1998
Human Human Foreskin microvascular endothelial Trypsin: 0.3%
HBSS Wojta J, Gallicchio M, Zoellner H, Filonzi E, Hamilton J, McGrath K: Interleukin-4 stimulates expression of urokinase-type-plasminogen activator in cultured human foreskin microvascular endothelial cells, Blood 81, 3285-92, 1993
Human Human Endothelial Trypsin: 0.3.%
(see reference)
Lee, K, Lawley, T, Xu, Y, and Swerlick, R.: VCAM-1-, ELAM-1-, and ICAM-1-Independent Adhesion of Melanoma Cells to Cultured Human Dermal Microvascular Endothelial Cells, J Invest Dermatol 98, 79, 1992
Human Human Vascular endothelial Neutral Protease: 0.15%
Trypsin: 0.25%
M-199 Farber HW, Antonov AS, Romanov YA, Smirnov VN, Scarfo LM, Beer DJ: Cytokine secretion by human aortic endothelial cells is related to degree of atherosclerosis, Am J Physiol 262, H1088-95, 1992
Human Human Umbilical vein
Collagenase: 0.1%
Cord Buffer
(See Reference)
Grant, D.S., Lelkes, P.I., Fukuda, K., Kleinman, H.K.: Intracellular Mechanisms Involved in Basement Membrane Induced Blood Vessel Differentiation In Vitro, In Vitro Cell Dev Biol 27, 327, 1991
Human Human Crypt cells Collagenase: 125 u/ml
RMPI 1640 Whitehead, R., and Eeden, P.: A Method For the Prolonged Culture of Colonic Epithelial Cells, J Tiss Cul Meth 13, 103, 1991
Human Human Endothelial Collagenase Type 1: 0.2%
Medium 199 Fischer, E., Stingl, A., and Kirkpatrick, C.: Migration Assay for Endothelial Cells in Multiwells Application to Studies on the Effect of Opioids, J Immunol Methods 128, 235, 1990
Human Human Human umbilical vein endothelial cells Collagenase Type 2: 75 u/ml
M199 Muller WA, Ratti CM, McDonnell SL, Cohn ZA: A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular junctions, J Exp Med 170, 399-414, 1989
Human Human Microvessels Collagenase Type 4: 0.1%
DMEM Gerhart, D. Z., Broderius, M. A., and Drewes, L. R.: Cutlured Human and Canine Endothelial Cells from Brain Microvessels, Brain Res Bull 21, 785, 1988
Human Human Endothelial/HUVEC
Foreskin & umbilical cord
Trypsin: 0.3%
HBSS/PBS, Medium 199
(see reference)
Kubota, Y., Kleinman, H., Martin, G., and Lawley, T.: Role of Laminin and Basement Membrane in Morphological Differentiation of Human Endothelial Cells into Capillary-like Structures, J Cell Biol 107, 1589, 1988
Human Human Umbilical cord
Smooth muscle
Collagenase: 0.1%
HEPES Hoshi, H., Kan, M., Chen, J., and McKeehan, W.: Comparative Endocrinology-Paracrinology-Autocrinology of Human Adult Large Vessel Endothelial and Smooth Muscle Cells, In Vitro Cell Dev Biol 24 (4), 309, 1988
Human Human Endothelial
Saphenous vein
Collagenase Type 2: 0.1%
PBS, CMF Sharefkin, J.B., Fairchild, K.D., Albus, R.A., Cruess, D.F., and Rich, N.M.: The Cytoxic Effect of Surgical Glove Powder Particles on Adult Human Vascular Endothelial Cell Cultures: Implications for Clinical Uses of Tissue Culture Techniques, J Surg Res 41, 463, 1986
Human Human Endothelial Collagenase: 0.1%
HEPES Hoshi, H., and McKeehan, W.: Isolation, Growth Requirements, Cloning, Prostacylclin Production and Life-Span of Human Adult Endothelial Cells in Low Serum Culture Medium, In Vitro Cell Dev Biol 22 (1), 51, 1986
Human Human Endothelial
Trypsin: 0.3%
PBS Marks, R.M., Czerniecki, M., and Penny, R.: Human Dermal Microvascular Endothelial Cells: An Improved Method for Tissue Culture and Description of Some Singular Properties in Culture, In Vitro Cell Dev Biol 21, 627, 1985
Human Human Fibroblasts
Hyaluronidase: 0.10%
DMEM Gordon, P., Sussman, I., and Hatcher, V.: Long-Term Culture of Human Endothelial Cells, In Vitro 19, 661, 1983
Human Human Iliac arteries Collagenase: 0.25%
PBS w/Ca++, Mg++, & BSA
(see reference)
Glassberg, M., Bern, M., Coughlin, S., Haudenschild, C., Hoyer, L., and Antoniades, H.: Cultured Endothelial Cells Derived from the Human Iliac Arteries, In Vitro 18, 859, 1982
Human Human, adult Peripheral blood mononuclear
T cells
Collagenase: 0.25%
RPMI 1640 Ashida, E., Johnson, A., and Lipsky, P.: Human endothelial cell-lymphocyte interaction. Endothelial cells function as accessory cells necessary for mitogen-induced human T lymphocyte activation in vitro, J Clin Invest 67, 1490, 1981
Human Human Microvascular endothelial
Neonatal foreskins
Neutral Protease: at 1000 u/ml
Konigsberg's modification of HBSS
(See Reference)
Sherer, G., Fitzharris, T., Faulk, W., and LeRoy, E.: Cultivation of Microvascular Endothelial Cells from Human Preputial Skin, In Vitro 16, 675, 1980
Human Human (adult and child) Foreskin Collagenase: 0.5%
Dulbecco's MEM w/10% calf serum Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76, 5217, 1979
Human Human Umbilical vein
Trypsin: 100 g/ml
Tris-HCl,0.2 M Jaffe, E.A., Minick, C.R., Adelman, B., Becker, C.G., and Nachman, R.: Synthesis of Basement Membrane Collagen By Cultured Human Endothelial Cells, J Exp Med 144, 209, 1976
Human Human Umbilical vein Collagenase Type 1: 125 u/ml
Dulbecco's PBS Gimbrone Jr., M.A.: Culture of Vascular Endothelium, Prog Hemost Thromb 3, 1, 1976
Human Human Umbilical cord Collagenase: 0.1%
Dulbecco's PBS Gimbrone, M.A., Cotran, R.S., and Folkman, J.: Human Vascular Endothelial Cells in Culture: Growth and DNA Synthesis, J Cell Biol 60, 673, 1974
Human Human Umbilical cord Collagenase: 0.2%
Cord buffer
(See Reference)
Jaffe, E., Nachman, R., Becker, C., and Minick, C.: Culture of Human Endothelial Cells Derived from Umbilical Veins. Identification by Morphologic and Immunologic Criteria, J Clin Invest 52, 2745, 1973
Human Human Umbilical cord Trypsin NF 1:250: 0.125%
Saline, normal Lewis, L.J., Haok, J.C., Maca, R.D., and Fry, G.L.: Replication of Human Endothelial Cells in Culture, Science 181, 452, 1973
Human Human Umbilical cord Trypsin NF 1:250: 0.25%
CMF solution Fryer, D.G., Birnbaum, G., and Luttrell, C.N.: Human Endothelium in Cell Culture, J Atheroscler Res 6, 151, 1966
Mouse Mouse Coronary endothelial Collagenase Type 2: 0.1%
Neutral Protease: 0.6 u/ml
Kreb's Luo, S., Truong, A. and Makino, A.: Isolation of Mouse Coronary Endothelial Cells., J Vis Exp , , 2016
Mouse Mouse Endothelial lung Collagenase Type 1: 0.2%
DMEM Wang, J., Sun, C., Gerdes, N., Liu, C., Liao, M., Liu, J., Shi, M., He, A., Zhou, Y., Sukhova, G., Chen, H., Cheng, X., Kuzuya, M., Murohara, T., Zhang, J., Cheng, X., Jiang, M., Shull, G. and Rogers, S.: Interleukin 18 Function in Atherosclerosis is Mediated by the Interleukin 18 Receptor and the Na-Cl Co-Transporter., Nat Med 21, 820-6, 2015
Mouse Mouse Endothelial Collagenase Type 2: 0.1%
Neutral Protease: 0.25 u/ml
Deoxyribonuclease I: 0.0075%
DMEM Xiao, L., McCann, J. and Dudley, A.: Isolation and Culture Expansion of Tumor-specific Endothelial Cells., J Vis Exp , e53072, 2015
Mouse Mouse Cerebral artery endothelial Neutral Protease: 4 u/ml
Elastase: 1 u/ml
Collagenase Type 1: 120 u/ml
DMEM Kochukov, M., Balasubramanian, A., Abramowitz, J., Birnbaumer, L. and Marrelli, S.: Activation of Endothelial Transient Receptor Potential C3 Channel is Required for Small Conductance Calcium-Activated Potassium Channel Activation and Sustained Endothelial Hyperpolarization and Vasodilation of Cerebral Artery., J Am Heart Assoc 3, , 2014
Mouse Mouse Tumor endothelial Collagenase Type 1: 0.2%
HBSS Kazerounian, S., Gerald, D., Huang, M., Chin, R, Udayakumar, D, Zheng, N., O'Donnell, R., Perruzzi, C., Mangiante, L., Pourat, J., Phung, T., Bravo-Nuevo, A., Shechter, S., McNamara, S. and Duhadaway, K.: RhoB Differentially Controls Akt Function in Tumor Cells and Stromal Endothelial Cells During Breast Tumorigenesis., Cancer Res 73, 50, 2013
Mouse Mouse, 8-14 week Endothelial Collagenase Type 4: 0.2%
HBSS Imoukhuede, P. and Popel, A.: Expression of VEGF Receptors on Endothelial Cells in Mouse Skeletal Muscle., PLoS ONE 7, e44791, 2012
Mouse Mouse Endothelial Papain: 40 u/ml
Deoxyribonuclease I: 125 u/ml
DPBS Daneman, R., Zhou, L., Agalliu, D., Cahoy, J., Kaushal, A. and Barres, B.: The Mouse Blood-Brain Barrier Transcriptome: a New Resource for Understanding the Development and Function of Brain Endothelial Cells., PLoS ONE 5, e13741, 2010
Mouse Mouse, 7-10 week Liver endothelial Collagenase: 0.03%
DMEM Follenzi, A., Benten, D., Novikoff, P., Faulkner, L., Raut, S. and Gupta, S.: Transplanted Endothelial Cells Repopulate the Liver Endothelium and Correct the Phenotype of Hemophilia A Mice., J Clin Invest 118, 935, 2008
Mouse Mouse, 4 week Endothelial kidney Collagenase Type 1: 0.1%
DMEM Kondo, S., Scheef, E., Sheibani, N. and Sorenson, C.: PECAM-1 Isoform-Specific Regulation of Kidney Endothelial Cell Migration and Capillary Morphogenesis., Am J Physiol Cell Physiol 292, C2070, 2007
Mouse Mouse, embryonic or yolk sac Endothelial Collagenase Type 3: 200 u/ml
Deoxyribonuclease I: 0.001%
PBS Braren Rickmer, Hu Huiqing, Kim YungHae, Beggs HilaryE, Reichardt LouisF, Wang Rong: Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation, J Cell Biol 172, 151-62, 2006
Mouse Mouse, neonatal Microvascular endothelial Neutral Protease: 0.005%
Collagenase Type 1: 4%
DMEM Cha, S., Talavera, D., Demir, E., Nath, A. and Sierra-Honigmann, M.: A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin., Microvasc Res 70, 198, 2005
Mouse Mouse, 4 week Retinal endothelial cells Collagenase Type 1: 0.1%
DMEM Su X, Sorenson CM, and Sheibani N.: Isolation and characterization of murine retinal endothelial cells, Mol Vis 9, 171, 2003
Mouse Mouse, male Endothelial cells from lymph node Collagenase Type 1: 0.1%
PBS Izawa D, Tanaka T, Saito K, Ogihara H, Usui T, Kawamoto S, Matsubara K, Okubo K, and Miyasaka M: Expression profile of active genes in mouse lymph node high endothelial cells, Int Immunol 11(12), 1989, 1999
Mouse Mouse, 2-4 week Cerebrovascular Endothelial Collagenase/Dispase: 0.1%
PBS Sapatino, B., Welsh, C., Smith, C., Bebo, B., and Linticum, D.: Cloned Mouse Cerebrovascular Endothelial Cells That Maintain Their Differentiation Markers for Factor VIII, Low Density Lipoprotein, and Angiotensin-Converting Enzyme, In Vitro Cell Dev Biol 29A, 923, 1993
Porcine Porcine, 6 month Valvular endothelial Neutral Protease: 2 u/ml
Collagenase Type 2: 60 u/ml
PBS Balaoing, L., Post, A., Lin, A., Tseng, H., Moake, J. and Grande-Allen, K.: Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation., PLoS ONE 10, e0130749, 2015
Porcine Porcine Valvular endothelial Collagenase Type 2: 300-600 u/ml
DMEM Gould, R. and Butcher, J.: Isolation of Valvular Endothelial Cells., J Vis Exp 46, e2158, 2010
Porcine Porcine
(also bovine)
Endothelial Collagenase Type 1:
DMEM w/ 10% calf serum Nugent, H., and Edelman, E.: Endothelial Implants Provide Long-Term Control of Vascular Repair in a Porcine Model of Arterial Injury, J Surg Res 99, 228, 2001
Porcine Porcine HUVEC, porcine pulmonary arterial endothelial cells Collagenase Type 2: 0.2%
DMEM Kwak HJ, Lee SJ, Lee YH, Ryu CH, Koh KN, Choi HY, and Koh GY.: Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells, Circulation 101(19), 2317, 2000
Porcine Porcine, 6-7 month Porcine pulmonary endothelial Collagenase Type 1: 0.3%
RPMI 1640 Hill-Kapturczak N, Kapturczak MH, Block ER, Patel JM, Malinski T, Madsen KM, and Tisher CC.: Angiotensin II-stimulated nitric oxide release from porcine pulmonary endothelium is mediated by angiotensin IV, J Am Soc Nephrol 10(3), 481, 1999
Porcine Porcine Endothelial Trypsin: 0.25%
Medium 199 Shasby, S.: Endothelial Cells Grown On Permeable Membrane Supports, J Tiss Cul Meth 14, 247, 1992
Porcine Porcine Endothelial Trypsin: 0.05%
DMEM Vischer, P., and Buddecke, E.: Alteration of Glycosytransferase Activities during Proliferation of Cultivated Arterial Endothelial Cells and Smooth Muscle Cells, Exp Cell Res 158, 15, 1985
Porcine Porcine Endothelial
Collagenase Type 2: 0.1%
Dulbecco-Vogt MEM w/o serum Dickinson, E. and Slakey, L.: Plasma-derived Serum as a Selective Agent to Obtain Endothelial Cultures from Swine Aorta, In Vitro 18, 63, 1982
Porcine Porcine, 30-40 kg Endothelia
Collagenase Type 4: 0.025%
Medium 199 Merrilees, M.J., and Scott, L.: Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture, Atherosclerosis 39, 147, 1981
Porcine Porcine, 20-30 week Endothelial
Collagenase: 0.1%
Medium 199 w/BSS and HEPES or NaHCO3 Slater, D.N., and Sloan, J.M.: The Porcine Endothelial Cell in Tissue Culture, Atherosclerosis 21, 259, 1975
Porcine Porcine, 60-100 days Aorta Trypsin: 0.1%
Phosphate buffer
(see reference)
Coulson, W.F.: The Effect Of Proteolytic Enzymes on the Tensile Strength of Whole Aorta and Isolated Aortic Elastin, Biochim Biophys Acta 237, 378, 1971
Rabbit Rabbit Corneal endothelial cells (CEC) Hyaluronidase: 0.05%
DMEM Choi, J., Ko, M., and Kay, E.: Subcellular Localization of the Expressed 18 kDa FGF-2 Isoform in Corneal Endothelial Cells, Mol Vis 6, 222, 2000
Rabbit Rabbit, 2-3Kg Endothelial, aortic Elastase: 0.2%
Hanks solution Haley, N., Shio, H., Fowler, S.: Characterization of lipid-laden aortic cells from cholesterol-fed rabbits. I. Resolution of aortic cell populations by metrizamide density gradient centrifugation, Lab Invest 37, 287, 1977
Rat Rat, SD, male, 250-300 g Smooth muscle, aorta Soybean Trypsin Inhibitor: 0.25%
HBSS with 0.2 mM Ca++ Schwertschlag, U.S., and Whorton, A.R.: Platelet-Activating Factor-Induced Homologous and Heterologous Desensitization In Cultured Vascular Smooth Muscle Cells, J Biol Chem 263, 13791, 1988
Rat Rat, SD, male, 350 - 450 g Lipocytes
Sinusoidal endothelial
Collagenase: 0.015%
DMEM/Ham's F-12 Friedman, S. and Roll, F.: Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan, Anal Biochem 161, 207, 1987
Rat Rat, Wistar, male, 3 mo Endothelial
Pronase: 0.25%
HBSS Nagelkenke, J., Barto, K., and VanBerkel, T.: In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells, J Biol Chem 258, 12221, 1983
Rat Rat, Wistar-Kyoto, male, 100 - 200 g Endothelial
Collagenase Type 2: 0.05%
HBSS Diglio, C.A., Grammas, P., Filiberto Giacomelli, M.S., and Wiener, J.: Primary Culture of Rat Cerebral Microvascular Endothelial Cells, Lab Invest 46, 554, 1982
Rat Rat, 300 G, and pig, 30-40 Kg Endothelial
Thoracic aorta
Trypsin: 0.05%
Medium 199 and 0.01M EDTA Merrilees, M.J., and Scott, L.: Interaction of aortic endothelial and smooth muscle cells in culture. Effect on glycosaminoglycan levels, Atherosclerosis 39, 147, 1981
Rat Rat Foreskin Collagenase: 0.5%
Dulbecco's MEM w/10% calf serum Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76, 5217, 1979
Rat Rat, Wistar, adult, 170 g Endothelial Trypsin: 0.5%
BSS Phillips, P., Kumar, P., Kumar, S., and Waghe, M.: Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter, J Anat 129, 261, 1979