Eye - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Microvascular endothelial Collagenase/Dispase: 0.1%
 MEM Bowman, P., Betz, A., and Goldstein, G.: Primary Culture of Microvascular Endothelial Cells From Bovine Retina, In Vitro 18 (7), 626, 1982
Chicken Chick, embryo, 6 day Retinal cells Trypsin: 0.005%
Deoxyribonuclease I: 0.005%
 DMEM/F12 Jacob Vanessa, Rothermel Andrae, Wolf Peter, Layer Paul G: Rhodopsin, violet and blue opsin expressions in the chick are highly dependent on tissue and serum conditions, Cell Death Differ. 180, 159-68, 2005
Chicken Chick embryo Corneal epithelia Collagenase: 0.08%
Trypsin: 0.08%
 HBSS Reenstra Wende R, Orlow Daniel L, Svoboda Kathy K H: ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent, Inv. Ophthal. Visual Sci. 43, 3181-9, 2002
Chicken Chick embryo
(also rat)		 Retinal Trypsin: 0.6%
 DMEM Seigel, G.: The Golden Age of Retinal Cell Culture, Mol. Vis. 5, 4, 1999
Chicken Chick, embryo, 10-14 day Flat, retina Trypsin: 0.1%
 Tyrode's solution, CMF Moyer, M., Bullrich, F., and Sheffield, J.: Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina, In Vitro Cell. Dev. Biol. 26, 1073, 1990
Human Human, 18-68 yr Corneal endothelial Collagenase: 0.2%
Neutral Protease: 1.0%
 DMEM/F12 Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G: A novel method of isolation, preservation, and expansion of human corneal endothelial cells, Inv. Ophthal. Visual Sci. 48, 614-20, 2007
Human Human Corneal limbal epithelial sheet Neutral Protease: 5%
 see reference Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R, and Tseng SC: Novel enzymatic isolation of an entire viable human limbal epithelial sheet, Inv. Ophthal. Visual Sci. 44(10), 4275, 2003
Human Human, 5-65 years Retinal pigment epithelial (RPE) Trypsin: 0.25%
 HBSS Von Recum, H., Okano, T., Kim, S, and Bernstein, P.: Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture, Exp. Eye Res. 69, 97, 1999
Monkey Monkey, cynomolgus, young adult Conjunctival lymphocytes Collagenase Type 1: 0.02%
 RPMI 1640 Whittum-Hudson JA, Taylor HR: Antichlamydial specificity of conjunctival lymphocytes during experimental ocular infection, Infect. Immun. 57, 2977, 1989
Mouse mouse, adult Retinal Papain: 50 u/ml
 DMEM/F-12 Jadhav AP, Cho SH and Cepko CL: Notch activity permits retinal cells to progress through multiple progenitor states and acquire a stem cell property, Proc. Natl. Acad. Sci. USA 103, 18998, 2006
Mouse Mouse, 2-5 month Mouse retinal and bipolar Papain: 20 u/ml
Deoxyribonuclease I: 200 u/ml
 HBSS Maxeiner Stephan, Dedek Karin, Janssen-Bienhold Ulrike, Ammermuller Josef, Brune Hendrik, Kirsch Taryn, Pieper Mario, Degen Joachim, Kruger Olaf, Willecke Klaus, Weiler Reto: Deletion of connexin45 in mouse retinal neurons disrupts the rod/cone signaling pathway between AII amacrine and ON cone bipolar cells and leads to impaired visual transmission, J. Neurosci. 25, 566-76, 2005
Mouse Mouse, 4 week Retinal endothelial cells Collagenase Type 1: 0.1%
 DMEM Su X, Sorenson CM, and Sheibani N.: Isolation and characterization of murine retinal endothelial cells, Mol. Vis. 9, 171, 2003
Porcine Porcine, 5-60 kg Retinal pigment epithelial cells Collagenase: 2%
 DMEM Wiencke, A., Kiilgaard, J., Nicolini, J., Bundgaard, M., Ropke, C., and La Cour, M.: Growth of Cultured Porcine Retinal Pigment Epithelial Cells, Acta Opthalmol. Scand. 81(2), 170, 2003
Rabbit Rabbit, New Zealand Corneal keratocytes Trypsin: 0.25%
Collagenase: 0.5%
 PBS Stramer Brian M, Kwok Michael G K, Farthing-Nayak Pamela J, Jung Jae-Chang, Fini M Elizabeth, Nayak Ramesh C: Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes, Inv. Ophthal. Visual Sci. 45, 807-12, 2004
Rabbit Rabbit, adult Retinal neurons Papain: 26 u/ml
 DMEM Brockway LM, Zhou Z-H, Bubien JK, Jovov B, Benos DJ, Keyser KT: Rabbit retinal neurons and glia express a variety of ENaC/DEG subunits, Am. J. Physiol. Cell Physiol. 283, C126-34, 2002
Rabbit Rabbit, New Zealand, adult, male, albino,
2 kg		 Epithelial Trypsin: 0.25%
 HBSS/DMEM Johnson-Muller, B., and Gross, J.: Regulation of Corneal Collagenase Production: Epithelial-Stromal Cell Interactions, Proc. Natl. Acad. Sci. USA 75 (9), 4417, 1978
Rat Rat, Dark Agouti, 3-4 week Ciliary-derived eye PDS kit: with modifications
 DMEM/F12 Akagi T, Mandai M, Ooto S, Hirami Y, Osakada F, Kageyama R, Yoshimura N, Takahashi M.: Otx2 homeobox gene induces photoreceptor-specific phenotypes in cells derived from adult iris and ciliary tissue, Inv. Ophthal. Visual Sci. 45, 4570, 2004
Rat Rat, embryonic 17 day Retinal Trypsin: 0.6%
 DMEM Seigel, G.: The Golden Age of Retinal Cell Culture, Mol. Vis. 5, 4, 1999
Rat Rat, SD Retina Papain: 120 U
 HBSS, PBS Jing, S., Wen, D., Yu, Y., Holst, P., Luo, Y., Fang, M., Tamir, R., Antonio, L., Hu, Z., et al.: GDNF-Induced Activation of the Ret Protein Tyrosine Kinase is Mediated by GDNFR-a, a Novel Receptor for GDNF, Cell 85, 1113, 1996
Rat Rat, 4-14 week Retinal pigment epithelial cells Collagenase Type 1: 65 u/ml
Hyaluronidase: 220 u/ml
 CF Hanks with EDTA Wang N, Koutz CA, and Anderson RE.: A method for the isolation of retinal pigment epithelial cells from adult rats, Inv. Ophthal. Visual Sci. 34(1), 101, 1993
Rat Rat, 6-8 day Retinal pigment epithelial Neutral Protease: 2%
 DMEM Chang CW, Roque RS, Defoe DM, and Caldwell RB.: An improved method for isolation and culture of pigment epithelial cells from rat retina, Curr. Eye Res. 10(11), 1081, 1991
Rat Rat, Long Evans, 1-15 days Neurons, visual cortex Papain: 20 u/ml
 BSS see reference (see reference) Huettner, J., and Baughman, R.: Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats, J. Neurosci. 6, 3044, 1986
Rat Rat, SD, pups Retina Trypsin: 0.25%
 Ham's F-12 Sarthy PV, Curtis BM, and Catterall WA.: Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat, J. Neurosci. 3 (12), 2532, 1983
Salamander Salamander, 18-25 cm Retina Papain: 14 u/ml
 Saline Townes-Anderson, E., MacLeish, P., and Raviola, E.: Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase, J. Cell Biol. 100, 175, 1985
Salamander Salamander (A. tigrinum) Photoreceptors, retina Papain: 0.05%
 (see reference) Bader, C., MacLeish, P., and Schwartz, E.: Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina, Proc. Natl. Acad. Sci. USA 75, 3507, 1978
Turtle Turtle (Pseudemys scripta elegans) Retinal Papain: 0.1% (13.5 u/mg)
 Kreb's Ringer Lam, D.: Biosynthesis of Acetylcholine in Turtle Photoreceptors, Proc. Natl. Acad. Sci. USA 69, 1987, 1972