Eye - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Pericyte Collagenase: 0.2%
DMEM Bryan, B. and D'Amore, P.: Pericyte Isolation and USe is Endothelial/Pericyte Coculture Models, Meth Enzymol 443, 315, 2008
Bovine Bovine Microvascular endothelial Collagenase/Dispase: 0.1%
MEM Bowman, P., Betz, A., and Goldstein, G.: Primary Culture of Microvascular Endothelial Cells From Bovine Retina, In Vitro 18 (7), 626, 1982
Chicken Chick, embryo, 6 day Retinal cells Trypsin: 0.005%
Deoxyribonuclease I: 0.005%
DMEM/F12 Jacob Vanessa, Rothermel Andrae, Wolf Peter, Layer Paul G: Rhodopsin, violet and blue opsin expressions in the chick are highly dependent on tissue and serum conditions, Cell Death Differ 180, 159-68, 2005
Chicken Chick embryo Corneal epithelia Collagenase: 0.08%
Trypsin: 0.08%
HBSS Reenstra Wende R, Orlow Daniel L, Svoboda Kathy K H: ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent, Inv Ophthal Visual Sci 43, 3181-9, 2002
Chicken Chick embryo
(also rat)
Retinal Trypsin: 0.6%
DMEM Seigel, G.: The Golden Age of Retinal Cell Culture, Mol Vis 5, 4, 1999
Chicken Chick, embryo, 10-14 day Flat, retina Trypsin: 0.1%
Tyrode's solution, CMF Moyer, M., Bullrich, F., and Sheffield, J.: Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina, In Vitro Cell Dev Biol 26, 1073, 1990
Fish Fish, zebrafish Retinal Papain: 10 u/ml
L-15 Diekmann, H., Kalbhen, P. and Fischer, D.: Characterization of Optic Nerve Regeneration using Transgenic Zebrafish., Front Cell Neurosci 9, 118, 2015
Human Human Corneal stromal stem Neutral Protease: 1.2 u/ml
Collagenase: 0.1%
DMEM Du, Y., Roh, D., Funderburgh, M., Mann, M., Marra, K., Rubin, J., Li, X. and Funderburgh, J.: Adipose-Derived Stem Cells Differentiate to Keratocytes In Vitro., Mol Vis 16, 2680, 2010
Human Human, 18-68 yr Corneal endothelial Collagenase: 0.2%
Neutral Protease: 1.0%
DMEM/F12 Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G: A novel method of isolation, preservation, and expansion of human corneal endothelial cells, Inv Ophthal Visual Sci 48, 614-20, 2007
Human Human Corneal limbal epithelial sheet Neutral Protease: 5%
see reference Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R, and Tseng SC: Novel enzymatic isolation of an entire viable human limbal epithelial sheet, Inv Ophthal Visual Sci 44(10), 4275, 2003
Human Human, 5-65 years Retinal pigment epithelial (RPE) Trypsin: 0.25%
HBSS Von Recum, H., Okano, T., Kim, S, and Bernstein, P.: Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture, Exp Eye Res 69, 97, 1999
Monkey Macaques and baboon Retinal Papain: 20-40 u/ml
Deoxyribonuclease I: 400 u/ml
Ames' solution Han ,Y., Jacoby, R. and Wu, S.: Morphological and Electrophysiological Properties of Dissociated Primate Retinal Cells., Brain Res 875, 175, 2000
Monkey Monkey, cynomolgus, young adult Conjunctival lymphocytes Collagenase Type 1: 0.02%
RPMI 1640 Whittum-Hudson JA, Taylor HR: Antichlamydial specificity of conjunctival lymphocytes during experimental ocular infection, Infect Immun 57, 2977, 1989
Mouse Mouse, C57BL/6 Retinal astrocytes Papain: 16.5 u/ml
Deoxyribonuclease I: 0.004%
HBSS Blandford, S. and Baldridge, W.: The Effect of Glutamate Receptor Agonists on Mouse Retinal Astrocyte [Ca(2+)]i.,Biomed Res Int Vol. 2016,, , 8178162, 2016
Mouse Mouse, adult Retinal photoreceptor PDS kit: with modifications
EBSS Feodorova, Y., Koch, M., Bultman, S., Michalakis, S. and Solovei, I.: Quick and Reliable Method for Retina Dissociation and Separation of Rod Photoreceptor Perikarya from Adult Mice., MethodsX 2, 39, 2015
Mouse Mouse, 4-8 day Photoreceptors PDS kit: per instructions
Neurobasal Balmer, J., Zulliger, R., Roberti, S. and Enzmann, V.: Retinal Cell Death Caused by Sodium Iodate Involves Multiple Caspase-Dependent and Caspase-Independent Cell-Death Pathways., Int J Mol Sci 16, 15086-103, 2015
Mouse Mouse, embryonic and P0 Retinal Papain: 8 u/ml
Deoxyribonuclease I: 124 u/ml
DPBS Dvoriantchikova, G., Perea-Martinez, I., Pappas, S., Barry, A., Danek, D., Dvoriantchikova, X., Pelaez, D. and Ivanov, D.: Molecular Characterization of Notch1 Positive Progenitor Cells in the Developing Retina., PLoS ONE 10, e0131054, 2015
Mouse Mouse Vitreous cells Collagenase: 0.1%
Hyaluronidase: 0.03%
DMEM Iqbal, N., Xu, L., Devitt, C. and Skapek, S.: Isolation and Characterization of Mammalian Cells Expressing the Arf Promoter During Eye Development., Biotechniques 56, 239-49, 2014
Mouse Mouse, 6 week Retinal PDS kit: per instructions
DMEM Singh, M., Issa, P, Butler, R., Martin, C., Lipinski, D., Sekaran, S., Barnard, A. and Maclaren, R.: Reversal of End-Stage Retinal Degeneration and Restoration of Visual Bunction by Photoreceptor Transplantation., Proc Natl Acad Sci U S A , , 2013
Mouse Mouse Retinal ganglion CLSPA: 240 u/ml
Hyaluronidase: 0.2%
Ames Schmidt, T. and Kofuji, P.: An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells., J Vis Exp 47, 2367, 2011
Mouse Mouse, adult Cilary epithelial PDS kit: per instructions
EBSS Gualdoni, S., Baron, M., Lakowski, J., Decembrini, S., Pearson, R., Ali, R. and Sowden, J.: Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells., Curr Protoc Stem Cell Biol Chapter 1, Unit 1H.4, 2011
Mouse Mouse, 4-5 week Lens Fiber Collagenase Type 4: 0.125%
Protease: 0.5%
PBS Ebihara, L., Tong, J., Vertel, B., White, T. and Chen, T.: Properties of Connexin 46 Hemichannels in Dissociated Lens Fiber Cells., Inv Ophthal Visual Sci 52, 882-9, 2011
Mouse Mouse, postnatal day 1 Retinal progenitor Collagenase: 0.1%
HBSS Jiang, C. Klassen, H., Zhang, X. and Young, M.: Laser Injury Promotes Migration and Integration of Retinal Progenitor Cells into Host Retina., Mol Vis 16, 983, 2010
Mouse Mouse Retinal Collagenase: 0.1%
Deoxyribonuclease I: 0.001%
RPMI Amadi-Obi, A., Yu, C., Liu, X., Mahdi, R., Clarke, G., Nussenblatt, R, Gery, I., Lee, Y. and Egwuagu, C.: TH17 Cells Contribute to Uveitis and Scleritis and are Expanded by IL-2 and Inhibited by IL-27/STAT1., Nat Med 13, 711, 2007
Mouse mouse, adult Retinal Papain: 50 u/ml
DMEM/F-12 Jadhav AP, Cho SH and Cepko CL: Notch activity permits retinal cells to progress through multiple progenitor states and acquire a stem cell property, Proc Natl Acad Sci U S A 103, 18998, 2006
Mouse Mouse, 2-5 month Mouse retinal and bipolar Papain: 20 u/ml
Deoxyribonuclease I: 200 u/ml
HBSS Maxeiner Stephan, Dedek Karin, Janssen-Bienhold Ulrike, Ammermuller Josef, Brune Hendrik, Kirsch Taryn, Pieper Mario, Degen Joachim, Kruger Olaf, Willecke Klaus, Weiler Reto: Deletion of connexin45 in mouse retinal neurons disrupts the rod/cone signaling pathway between AII amacrine and ON cone bipolar cells and leads to impaired visual transmission, J Neurosci 25, 566-76, 2005
Mouse Mouse, 4 week Retinal endothelial cells Collagenase Type 1: 0.1%
DMEM Su X, Sorenson CM, and Sheibani N.: Isolation and characterization of murine retinal endothelial cells, Mol Vis 9, 171, 2003
Porcine Porcine, 5-60 kg Retinal pigment epithelial cells Collagenase: 2%
DMEM Wiencke, A., Kiilgaard, J., Nicolini, J., Bundgaard, M., Ropke, C., and La Cour, M.: Growth of Cultured Porcine Retinal Pigment Epithelial Cells, Acta Opthalmol Scand 81(2), 170, 2003
Rabbit Rabbit, New Zealand, Adult Corneal Endothelial Collagenase Type 2: 0.2%
HBSS Lai, J., Cheng, H. and Ma, D.: Investigation of Overrun-Processed Porous Hyaluronic Acid Carriers in Corneal Endothelial Tissue Engineering, PLoS ONE 10, 1371, 2015
Rabbit Rabbit Corneal keratocytes Hyaluronidase: 0.05%
Collagenase: 0.2%
DMEM Hao, M., Flynn, K., Nien-Shy, C., Jester, B., Winkler, M., Brown, D., La Schiazza, O., Bille, J., Jester, J.: In Vivo Non Linear Optical (NLO) Imaging in Live Rabbit Eyes Using the Heidelbert Two-Photon Laser Opthalmoscope, Exp Eye Res 91, 308, 2010
Rabbit Rabbit, New Zealand Corneal keratocytes Trypsin: 0.25%
Collagenase: 0.5%
PBS Stramer Brian M, Kwok Michael G K, Farthing-Nayak Pamela J, Jung Jae-Chang, Fini M Elizabeth, Nayak Ramesh C: Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes, Inv Ophthal Visual Sci 45, 807-12, 2004
Rabbit Rabbit, adult Retinal neurons Papain: 26 u/ml
DMEM Brockway LM, Zhou Z-H, Bubien JK, Jovov B, Benos DJ, Keyser KT: Rabbit retinal neurons and glia express a variety of ENaC/DEG subunits, Am J Physiol Cell Physiol 283, C126-34, 2002
Rabbit Rabbit, New Zealand, adult, male, albino,
2 kg
Epithelial Trypsin: 0.25%
HBSS/DMEM Johnson-Muller, B., and Gross, J.: Regulation of Corneal Collagenase Production: Epithelial-Stromal Cell Interactions, Proc Natl Acad Sci U S A 75 (9), 4417, 1978
Rat Rat, Wister, 3-30 wk Retinal Deoxyribonuclease I: 0.005%
Papain: 30 u/ml
EBSS Cameron, M., Kekesi, O., Morley, J., Tapson, J., Breen, P., Van Schaik, A and Buskila, Y.: Calcium Imaging of AM Dyes Following Prolonged Incubation in Acute Neuronal Tissue., PLoS ONE 11, e0155468, 2016
Rat Rat, SD, 6-8 week Retinal PDS kit: per instructions
EBSS Vigneswara, V., Esmaeili, M., Deer, L., Berry, M., Logan, A. and Ahmed, Z.: Eye Drop Delivery of Pigment Epithelium-Derived Factor-34 Promotes Retinal Ganglion Cell Neuroprotection and Axon Regeneration., Mol Cell Neurosci 68, 212-21, 2015
Rat Rat, SD Retinal ganglion PDS kit: with modifications
Neurobasal Tan, C., Andrews, M., Kwok, J., Heintz, T., Gumy, L., Fassler, R. and Fawcett, J.: Kindlin-1 Enhances Axon Growth on Inhibitory Chondroitin Sulfate Proteoglycans and Promotes Sensory Axon Regeneration., J Neurosci 32, 7325, 2012
Rat Rat, SD, 180-220g Retinal ganglion Papain: 0.2%
Neurobasal Ma, J., Yu, W., Wang, Y., Cao, G., Cai, S., Chen, X., Yan, N., Yuan, Y., Zeng, H., Fleenor, D., Liu, X. and Pang, I.: Neuroprotective Effects of C-type Natriuretic Peptide on Rat Retinal Ganglion Cells., Inv Ophthal Visual Sci 51, 3544-53, 2010
Rat Rat, Fisher, adult Retinal PDS kit: per instructions
MEM Suzuki, T., Mandai, M., Akimoto, M., Yoshimura, N. and Takahashi, M.: The Simultaneous Treatment of MMP-2 Stimulants in Retinal Transplantation Enhances Grafted Cell Migration into the Host Retina., Stem Cells 24, 2406, 2006
Rat Rat, Dark Agouti, 3-4 week Ciliary-derived eye PDS kit: with modifications
DMEM/F12 Akagi T, Mandai M, Ooto S, Hirami Y, Osakada F, Kageyama R, Yoshimura N, Takahashi M.: Otx2 homeobox gene induces photoreceptor-specific phenotypes in cells derived from adult iris and ciliary tissue, Inv Ophthal Visual Sci 45, 4570, 2004
Rat Rat, embryonic 17 day Retinal Trypsin: 0.6%
DMEM Seigel, G.: The Golden Age of Retinal Cell Culture, Mol Vis 5, 4, 1999
Rat Rat, SD Retina Papain: 120 U
HBSS, PBS Jing, S., Wen, D., Yu, Y., Holst, P., Luo, Y., Fang, M., Tamir, R., Antonio, L., Hu, Z., et al.: GDNF-Induced Activation of the Ret Protein Tyrosine Kinase is Mediated by GDNFR-a, a Novel Receptor for GDNF, Cell 85, 1113, 1996
Rat Rat, 4-14 week Retinal pigment epithelial cells Collagenase Type 1: 65 u/ml
Hyaluronidase: 220 u/ml
CF Hanks with EDTA Wang N, Koutz CA, and Anderson RE.: A method for the isolation of retinal pigment epithelial cells from adult rats, Inv Ophthal Visual Sci 34(1), 101, 1993
Rat Rat, 6-8 day Retinal pigment epithelial Neutral Protease: 2%
DMEM Chang CW, Roque RS, Defoe DM, and Caldwell RB.: An improved method for isolation and culture of pigment epithelial cells from rat retina, Curr Eye Res 10(11), 1081, 1991
Rat Rat, Long Evans, 1-15 days Neurons, visual cortex Papain: 20 u/ml
BSS
(see reference)
Huettner, J., and Baughman, R.: Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats, J Neurosci 6, 3044, 1986
Rat Rat, SD, pups Retina Trypsin: 0.25%
Ham's F-12 Sarthy PV, Curtis BM, and Catterall WA.: Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat, J Neurosci 3 (12), 2532, 1983
Salamander Salamander Neurons Papain: 14 u/ml
Ringers Clarke, R, Wang, J. and Townes-Anderson, E.: Using Laser Tweezers For Manipulating Isolated Neurons In Vitro., J Vis Exp 19, 911, 2008
Salamander Salamander, 18-25 cm Retina Papain: 14 u/ml
Saline Townes-Anderson, E., MacLeish, P., and Raviola, E.: Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase, J Cell Biol 100, 175, 1985
Salamander Salamander (A. tigrinum) Photoreceptors, retina Papain: 0.05%
(see reference) Bader, C., MacLeish, P., and Schwartz, E.: Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina, Proc Natl Acad Sci U S A 75, 3507, 1978
Turtle Turtle (Pseudemys scripta elegans) Retinal Papain: 0.1% (13.5 u/mg)
Kreb's Ringer Lam, D.: Biosynthesis of Acetylcholine in Turtle Photoreceptors, Proc Natl Acad Sci U S A 69, 1987, 1972