Heart - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine
(also rat)		 Heart Adrenal chromaffin Paraneurons Trypsin: 0.06%
 25mM HEPES buffered Locke's solution, CMF Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990
Canine Dog, mongrel, adult Myocytes Hyaluronidase: 0.1%
 Joklik's MEM Spanier, A. and Weglicki, W.: Ca2+-Tolerant Adult Canine Myocytes: Preparation and Response to Anoxia/Acidosis, Am J Physiol 243, H448, 1982
Chicken Chicken embryos, 9-11 day incubated Cardiomyocytes Trypsin: 0.25%
 PBS Eschenhagen, T., Fink, C., Remmers, U., Scholz, H., Wattchow, J., Weil, J., et al.: Three-Dimensional Reconstitution of Embryonic Cardiomyocytes in a Collagen Matrix: A New Heart Muscle Model System, FASEB J 11, 683, 1997
Chicken Chick, embryos, 10-11 day Cardiomyocytes Trypsin: 0.17%
 HBSS, CMF Wang, S., Greaser, M.L., Schultz, E., Bulinski, J.C., Lin, J.J., and Lessard, J.L.: Studies on Cardiac Myofibrillogenesis With Antibodies to Titin, Actin, Tropomyosin, and Myosin, J Cell Biol 107, 1075, 1988
Chicken Chick, 10 day Ventricular Trypsin: 0.025%
 HBSS, CMF Kim, D., Okada, A., and Smith, T.W.: Control of Cytosolic Calcium Activity During Low Sodium Exposure in Cultured Chick Heart Cells, Circ Res 61, 29, 1987
Chicken Chick, embryo, 11 day Cardiomyocytes Trypsin: 0.05%
 HBSS, CMF Jacob, R., Lieberman, M., Murphy, E., and Piwnica-Worms, D.: Effect of Sodium-Potassium Pump Inhibition and Low Sodium on Membrane Potential in Cultured Embryonic Chick Heart Cells, J Physiol 387, 549, 1987
Chicken Chick, embryonic, 11-12-day-old Myocytes Trypsin: 0.025%
 CMF solution Murphy, E., Aiton, J., Russell, C., and Lieberman, M.: Calcium Elevation in Cultured Heart Cells: Its Role in Cell Injury, Am J Physiol Cell Physiol 245 (14), C316, 1983
Chicken Chick, 8 day embryo
(also rat, neonate and mouse)		 Myocytes Trypsin NF 1:250: 0.25%
 Rinaldini's buffer solution, CF Gross, W., Schopf-Ebner, E., and Bucher, O.: Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells, Exp Cell Res 53, 1, 1968
Chicken Hen, White Leghorn Heart Trypsin: 0.025%
 Medium 199 Dehann, R.: Regulation of Spontaneous Activity and Growth of Embryonic Chick Heart Cells in Tissue Culture, Dev Biol 16, 216, 1967
Chicken Chick embryos, 5 day Heart Liver Trypsin: 3.0%
 Tyrode's solution, CMF Steinberg, M.: "ECM": Its Nature, Origin, And Function in Cell Aggregation, Exp Cell Res 30, 257, 1963
Feline Cat, mongrel, adult, 1.8-2.8 Kg Myocytes Ventricular Collagenase Type 2: 0.12%
 Kreb's Henseleit, CF Silver, L., Hemwall, E., Marino, T., and Houser, S.: Isolation and Morphology of Calcium-Tolerant Feline Ventricular Myocytes, Am J Physiol 245, H891, 1983
Fish Octopus, E. cirhosa, 260-352 g Systemic heart cardiomyocytes Collagenase: 0.025%
Trypsin: 0.02%
 see reference Altimiras J., Hove-Madsen L., and Gesser H.: Ca(2+) Uptake in the Sarcoplasmic Reticulum from the Systemic Heart of Octopod Cephalopods, J Exp Biol 202, 2531, 1999
Frog Frog (Rana esculenta) Myocytes Trypsin: 0.04%
 CF Ringer Arrio-Dupont, M., and de Nay, D.: High Yield Preparation of Calcium-Tolerant Myocytes From Frog Ventricles, Biol Cell 54, 164, 1985
Guinea-Pig Guinea pig, adult Cardiomyocytes Collagenase Type 2: 100 u/ml
 M-199 Zorn-Pauly K, Schaffer P, Pelzmann B, Bernhart E, Lang P, and Koidl B: L-type and T-type Ca2+ current in cultured ventricular guinea pig myocytes, Physiol Res 53(4), 369, 2004
Guinea-Pig Guinea pig, female, 300-350g Coronary endothelial Collagenase Type 2: 0.1%
 see reference Buxton I L, Kaiser R A, Oxhorn B C, Cheek D J: Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium, Am J Physiol Heart Circ Physiol 281, H1657-66, 2001
Guinea-Pig Guinea pig, 250-450 g Endothelial Collagenase Type 2: 0.15%
 Perfusing solution
(see reference)		 Preisig-Muller, R., Mederos Y Schnitzler, M., Derst, C., and Daut J.: Separation of Cardiomyocytes and Coronary Endothelial Cells for Cell-Specific RT-PCR, Am J Physiol 277, H413, 1999
Guinea-Pig Guinea-pig, male, 450-600 g Myocytes Hyaluronidase: 0.02%
 Krebs Henseleit bicarbonate buffer Stemmer, P., Akera,T., Brody, T., Rardon, D., and Watanabe, A.: Isolation and Enrichment of Ca2+-Tolerant Myocytes for Biochemical Experiments from Guinea-Pig Heart, Life Sci 44, 1231, 1989
Guinea-Pig Guinea-pig Cardiomyocytes Collagenase: 0.04%
 Tyrode solution, CF Ishihara, K., Mitsuiye, T., Noma, A., and Takano, M.: The Mg2+ Block and Intrinsic Gating Underlying Inward Rectification of the K+ Current in Guinea-Pig Cardiac Myocytes, J Physiol 419, 297, 1989
Guinea-Pig Guinea-pig Myocytes Hyaluronidase: 0.10%
 Bicarbonate buffer, CF Bridge, J., Spitzer, K., and Ershler, P.: Relaxation of Isolated Ventricular Cardiomyocytes by a Voltage-Dependent, Science 241, 823, 1988
Human Human (n=16, age: 60 &plusmin;3 years) Myocardial Hyaluronidase: 0.05%
 Medium 199 Mukerjee, R., Multani, M., Sample, J., Dowdy, K., Zellner, J., Hoover, D., and Spinale, F.: Effects of adrenomedullin on human myocyte contractile function and beta-adrenergic response, J Cardiovasc Pharmacol 7 (4), 235, 2002
Human Human Cardiomyocytes Collagenase Type 2: 0.05%
Collagenase Type 1: 0.025%
Protease XIV: 0.013%
 HEPES solution Todor Anastassia, Sharov Victor G, Tanhehco Elaine J, Silverman Norman, Bernabei Alvise, Sabbah Hani N: Hypoxia-induced cleavage of caspase-3 and DFF45/ICAD in human failed cardiomyocytes, Am J Physiol Heart Circ Physiol 283, H990-5, 2002
Human Human Heart Myocytes Collagenase Type 2: 200 u/ml
 Tyrode's solution Hoppe, U., Jansen, E., Sudkamp, M., and Beukelmann, D.: Hyperpolarization-Activated Inward Current in Ventricular Myocytes From Normal and Failing Human Hearts, Circulation 97, 55, 1998
Human Human, fetal, 14.5 week gestation Myocytes Hyaluronidase: 0.1%
 HBSS with Calcium Goldman, B., and Wurzel, J.: Effects of Subcultivation and Culture Medium on Differentiation of Human Fetal Cardiac Myocytes, In Vitro Cell Dev Biol 28, 109, 1992
Human Human, both sexes, 18-81 years Myocardial Atrial Collagenase: 0.14%
 HBSS, CMF Smith, D., Glover, J., Townsend, L., and Maupin, E.: A Method for the Harvest, Culture, and Characterization of Human Adult Atrial Myocardial Cells: Correlation with Age of Donor, In Vitro Cell Dev Biol 27, 914, 1991
Human Human, adult Myocytes Trypsin: 0.25%
 Joklik's MEM Bugaisky, L.B.: Biology of Isolated Adult Cardiac Myocytes,Isolation and Culture of Human Adult Cardiac Myocytes,(Clark,Decker,Borg,eds), Elsevier Science Publishing Co., Inc., , 1988
Human Human Thoracic aorta Collagenase: 0.2%
 Phosphate buffer w/NaCl Hassler, O., Wiren, M., and Herbertsson, S.: The Elastic Coat of the Arterial Wall Studied with the Aid of Collagenase, Acta Pathol Microbiol Scand 57, 15, 1963
Mouse Mouse, adult Cardiomyocytes Collagenase: see reference
 HBSS O'Connell Timothy D, Rodrigo Manoj C, Simpson Paul C: Isolation and culture of adult mouse cardiac myocytes, Methods Mol Biol 357, 271-96, 2007
Mouse Mouse Smooth muscle cells Collagenase Type 2: 300 u/ml
Elastase: 5 u/ml
 F10 Ham Verheye, S., Martinet, W., Kockx, M., Knaapen, M., Salu, K., Timmermans, J., Ellis, J., Kilpatrick, D., DeMayer, G.: Selective Clearance of Macrophages in Atherosclerotic Plaques by Autophagy, J Am Coll Cardiol 49, 706, 2007
Mouse Mouse Ventricular myocytes and mesenteric arterial SMC Collagenase Type 2: 0.06%
Papain: 0.175%
 Krebs-Ringer Lu Tong, Ye Dan, Wang Xiaoli, Seubert JohnM, Graves JoanP, Bradbury JAlyce, Zeldin DarrylC, Lee Hon-Chi: Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms, J Physiol 575, 627-44, 2006
Mouse Mouse, neonatal Cardiomyoctes Collagenase Type 1: 150 u/ml
Trypsin: 0.01%
 M199 Takahashi N, Wang X, Tanabe S, Uramoto H, Jishage K, Uchida S, Sasaki S, Okada Y.: ClC-3-independent sensitivity of apoptosis to Cl- channel blockers in mouse cardiomyocytes, Cell Physiol Biochem 15, 263, 2005
Mouse Mouse, 1 day Cardiomyoctes NCIS kit:
EBSS Potts, Malia B., Vaughn, Allyson E., McDonough, Holly, Patterson, Cam, and Deshmukh, Mohanish: Reduced Apaf-1 levels in cardiomyocytes engage strict regulation of apoptosis by endogenous XIAP, J Cell Biol 171, 925, 2005
Mouse Mouse Smooth muscle cells Collagenase Type 1: 0.1%
Elastase: 0.0125%
 DMEM Fukumoto Yoshihiro, Deguchi Jun-o, Libby Peter, Rabkin-Aikawa Elena, Sakata Yasuhiko, Chin Michael T, Hill Christopher C, Lawler Patrick R, Varo Nerea, Schoen Frederick J, Krane Stephen M, Aikawa Masanori: Genetically determined resistance to collagenase action augments interstitial collagen accumulation in atherosclerotic plaques, Circulation 110, 1953-9, 2004
Mouse Mouse, female, 14-18 g Cardiomyocytes Collagenase Type 2: 0.02%
Elastase: 0.03%
Pancreatin: 0.06%
 see reference Zhang Sui, Wang Dachun, Estrov Zeev, Raj Sean, Willerson James T, Yeh Edward T H: Both cell fusion and transdifferentiation account for the transformation of human peripheral blood CD34-positive cells into cardiomyocytes in vivo, Circulation 110, 3803-7, 2004
Mouse Mouse, 6-8 week Vascular smooth muscle Papain: 10 u/ml
Elastase: .005%
Collagenase: 0.05%
Deoxyribonuclease I: 1000 u/ml
PDS kit: with modifications
 EBSS Qian, Q., Hunter, L., Li, M., Marin-Padilla, M., Prakash, Y., Somlo, S., Harris, P., Torres, V., and Sieck, G.: Pkd2 Haploinsufficiency Alters Intracellular Calcium Regulation in Vascular Smooth Muscle Cells, Hum Mol Genet 12(15), 1875, 2003
Mouse Mouse Cardiomyocytes Collagenase Type 2: 0.2%
Pancreatin: 0.06%
 DMEM/F-12 Rybkin, I., Markham, D., Yan, Z., Bassel-Duby, R., Williams, R., and Olson, E.: Conditional Expression of SV40 T-antigen in Mouse Cardiomyocytes Facilitates an Inducible Switch from Proliferation to Differentiation, J Biol Chem 278(18), 15927, 2003
Mouse Mouse, neonatal 2-4 day Atrial myocytes NCIS kit: with modifications see reference
 L-15 Bettahi, I,, Marker, C., Roman, M., and Wickman, K.: Contribution of the Kir3.1 Subunit to the Muscarinic-gated Atrial Potassium Channel IKACh, J Biol Chem 277, 48282, 2002
Mouse Mouse, neonatal and adult Cardiomyocytes Collagenase Type 2: 0.03-0.2%
 Joklik's MEM Schreiber, K., Paquet, L., Allen, B., and Rindt, H.: Protein Kinase C Isoform Expression and Activity in the Mouse Heart, Am J Physiol Heart Circ Physiol 281(5), H2062, 2001
Mouse Mouse, NTg and Tg, 8 - 12 week Myocytes Collagenase Type 2: 150 u/ml
 Joklik's MEM Nelson, D., Setser, E., Hall, D., Schwartz, S., Hewitt, T., Klevitsky, R., Osinska, H., Bellgrau, D., Duke, R., and Robbins, J.: Proinflammatory Consequences of Transgenic Fas Ligand Expression in the Heart, J Clin Invest 105 (9), 1199, 2000
Mouse Mouse, 1-6 day Cardiomyocytes Collagenase Type 2: 0.1%
Deoxyribonuclease I: 0.002%
 PBS Watzka SB, Lucien J, Shimada M, Edwards V, Yeger H, Hannigan G, and Coles JG.: Selection of viable cardiomyocytes for cell transplantation using three-dimensional tissue culture, Transplantation 70, 1310, 2000
Mouse Mouse, 2-4 mo Ventricular myocytes Collagenase Type 2: 0.05%
Collagenase Type 4: 0.05%
Protease XIV: 0.002%
 MEM Zhou YY, Wang SQ, Zhu WZ, Chruscinski A, Kobilka BK, Ziman B, Wang S, Lakatta EG, Cheng H, Xiao RP: Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology, Am J Physiol Heart Circ Physiol 279, H429-36, 2000
Mouse Mouse Cardiomyocytes Collagenase Type 2: 150 u/ml
 Joklik's MEM Christensen G, Minamisawa S, Gruber PJ, Wang Y, Chien KR: High-efficiency, long-term cardiac expression of foreign genes in living mouse embryos and neonates, Circulation 101, 178-84, 2000
Mouse Mouse, 1-3 day Cardiomyocytes Trypsin: 0.25%
 FBS-MEM Wang, G., and Kang, Y.: Inhibition of Doxorubicin Toxicity in Cultured Neonatal Mouse Cardiomyocytes with Elevated Metallothionein Levels, J Pharmacol Exp Ther 288(3), 938, 1999
Mouse Mouse, 1 day Cardiomyocytes NCIS kit: with modifications see reference
 L-15 Lader, A., Kwiatkowski, D., and Cantiello, H.: Role of Gelsolin in the Actin Filament Regulation of Cardiac L-type Calcium Channels, Am J Physiol 277, C1277, 1999
Mouse Mouse, 3-7 month Fibroblasts, mesangial, smooth muscle Trypsin: 0.25%
Collagenase: see reference
Soybean Trypsin Inhibitor: .05%
 DMEM Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH: Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation, Mol Biol Cell 10, 1569-79, 1999
Mouse Mouse (BALB/c), adult Myocytes Collagenase Type 2: 0.04%
 Tyrode's solution Felzen, B., Shilkrut, M., Less, H., Sarapov, I., Maor, G., Coleman, R., Robinson, R., et al.: Fas (CD95/Apo-1)-Mediated Damage to Ventricular Myocytes Induced by Cytotoxic T Lymphocytes from Perforin-Deficient Mice, Circ Res 82, 438, 1998
Mouse Mouse, 1 day Cardiomyocytes NCIS kit: with modifications see reference
 L-15 Lader, A., Xiao, Y., Ishikawa, Y., Cui, Y., Vatner, D., Vatner, S., Homcy, C., Cantiello, H.: Cardiac Gsalpha Overexpression Enhances L-type Calcium Channels Through an Adenylyl Cyclase Independent Pathway, Proc Natl Acad Sci U S A 95, 9669, 1998
Mouse Mouse, P0-P1 Cardiomyocytes Collagenase: 0.045%
 DMEM, Medium 199 w/ 10% horse serum and 5% fetal calf serum Arber, S., Hunter, J., Ross, J., Hongo, M., Sansig, G., Borg, J., Perriard, J., Chien, K., and Caroni, P.: MLP-Deficient Mice Exhibit a Disruption of Cardiac Cytoarchitectural Organization, Dilated Cadiomyopathy, and Heart Failure, Cell 88, 393, 1997
Mouse Mouse, 6-9 or 21-41 day Ventricular and atrial myocytes Collagenase Type 2: 95 u/ml
Hyaluronidase: 172.5 u/ml
Trypsin: 0.002%
Deoxyribonuclease I: 60u/ml
 DMEM/Tyrodes Valenzuela, D., Han, X., Mende, U., Fankhauser, C., Mashimo, H., Huang, P., Pfeffer, J., Neer, E., and Fishman, M.: G alpha(o) is Necessary for Muscarinic Regulation of Ca2+ Channels in Mouse Heart, Proc Natl Acad Sci U S A 94(5), 1727, 1997
Mouse Mouse Cardiomyocyte Collagenase: 0.17 %
 PBS Soonpaa, M., Kim, K., Pajak, aL., Franklin, M., and Field, L.: Cardiomyocyte DNA Synthesis and Binucleation During Murine Development, Am J Physiol 271, H2183, 1996
Mouse Mouse embryos Myocytes Collagenase Type 1: 0.1%
 DMEM Wobus, A., Kleppisch, T., Maltsev, V., and Hescheler, J.: Cardiomyocyte-Like Cells Differentiated In Vitro From Embryonic Carcinoma Cells P19 are Characterized by Functional Expression of Adrenoceptors and Ca2+ Channels, In Vitro Cell Dev Biol 30A, 425, 1994
Mouse Mouse
(also rat, neonate and chick, 8 day embryo)		 Myocytes Trypsin NF 1:250: 0.25%
 Rinaldini's buffer solution, CF Gross, W., Schopf-Ebner, E., and Bucher, O.: Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells, Exp Cell Res 53, 1, 1968
Porcine Porcine Coronary vascular smooth muscle (PCVSMCs) Trypsin: 0.037%
 HEPES Christ, M., Gunther, A., Heck, M., Schmidt, B., Falkenstein, E., and Wehling, M.: Aldosterone, Not Estradiol, Is the Physiological Agonist for Rapid Increases in cAMP in Vascular Smooth Muscle Cells, Circulation 99, 1485, 1999
Porcine Miniature swine, adult, female, 25-40 kg, familiarized with treadmill exercise Coronary smooth muscle Trypsin: 0.1%
 Krebs bicarbonate solution, iced Bowles, D., Hu, Q., Laughlin, M., and Sturek, M.: Exercise Training Increases L-type Calcium Current Density in Coronary Smooth Muscle, Am J Physiol 275 (44), H2159, 1998
Rabbit Rabbit Smooth muscle cells Collagenase Type 2: 300 u/ml
Elastase: 5 u/ml
 F10 Ham Verheye, S., Martinet, W., Kockx, M., Knaapen, M., Salu, K., Timmermans, J., Ellis, J., Kilpatrick, D., DeMayer, G.: Selective Clearance of Macrophages in Atherosclerotic Plaques by Autophagy, J Am Coll Cardiol 49, 706, 2007
Rabbit Rabbit, New Zealand, 2 kg Cardiomyocytes and fibroblasts Collagenase: 0.06%
 Medium 199 Driesen Ronald B, Dispersyn Gerrit D, Verheyen Fons K, van den Eijnde Stefan M, Hofstra Leo, Thone Fred, Dijkstra Petra, Debie Wiel, Borgers Marcel, Ramaekers Frans C S: Partial cell fusion: a newly recognized type of communication between dedifferentiating cardiomyocytes and fibroblasts, Cardiovasc Res 68, 37-46, 2005
Rabbit Rabbit, New Zealand White, 2-3 kg Myocytes Collagenase Type 2: 0.1%
 HEPES Spitzer, K., Ershler, P., Skolnick, R., and Vaughan-Jones, R.: Generation of Intracellular pH Gradients in Single Cardiac Myocytes with a Microperfusion System, Am J Physiol 278, H1371, 2000
Rabbit Rabbit, New Zealand, white, either sex Myocytes Collagenase Type 2: 0.05%
 EGTA-KB Sedarat, F., Xu, L., Moore, E., and Tibbits, G.: Colocalization of Dihydropyridine and Ryanodine Receptors in Neonate Rabbit Heart Using Confocal Microscopy, Am J Physiol Heart Circ Physiol 279, H202, 2000
Rabbit Rabbit, adult 1.5-2.5 kg Tricuspid valve cells Collagenase: 0.8 mg/ml
 HEPES Tyrode solution Anumonwo JM, Delmar M, and Jalife J.: Electrophysiology of single heart cells from the rabbit tricuspid valve, J Physiol 425, 145, 1990
Rabbit Rabbit, New Zealand, white, male, 2-3 kg Cardiomyocytes Hyaluronidase: 0.5%
 Eagle's MEM Buxton, I., and Brunton, L.: Compartments of Cyclic AMP and Protein Kinase in Mammalian Cardiomyocytes, J Biol Chem 258 (17), 10233, 1983
Rabbit Rabbit, adult, 2 kg Myocytes Hyaluronidase: 0.007%
 Kreb's Ringer Dani, A., Cittadini, A., Flamini, G., Festuccia, G., and Terranova, T.: Preparation and Some Properties of Isolated Beating Myocytes from Adult Rabbit Heart, J Mol Cell Cardiol 9, 777, 1977
Rat Rat, SD, 300-350 g Cardiomyocytes Collagenase Type 2: 0.05%
Hyaluronidase: 0.02%
 DMEM Niederbichler Andreas D, Hoesel Laszlo M, Westfall Margaret V, Gao Hongwei, Ipaktchi Kyros R, Sun Lei, Zetoune Firas S, Su Grace L, Arbabi Saman, Sarma J Vidya, Wang StewartC, Hemmila MarkR, Ward PeterA: An essential role for complement C5a in the pathogenesis of septic cardiac dysfunction, J Exp Med 203, 53-61, 2006
Rat Rat, SD, male Ventricular myocytes Collagenase: 0.13%
Hyaluronidase: 0.06%
 DMEM Stagg Mark A, Coppen Steven R, Suzuki Ken, Varela-Carver Anabel, Lee Joon, Brand Nigel J, Fukushima Satsuki, Yacoub Magdi H, Terracciano Cesare M: Evaluation of frequency, type, and function of gap junctions between skeletal myoblasts overexpressing connexin43 and cardiomyocytes: relevance to cell transplantation, FASEB J 20, 744-6, 2006
Rat Rat, SD, 200-250 g Ventricular myocytes and mesenteric arterial SMC Collagenase Type 2: 0.06%
Papain: 0.175%
 Krebs-Ringer Lu Tong, Ye Dan, Wang Xiaoli, Seubert JohnM, Graves JoanP, Bradbury JAlyce, Zeldin DarrylC, Lee Hon-Chi: Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms, J Physiol 575, 627-44, 2006
Rat Rat, 1-2 day Cardiomyocytes NCIS kit: per instructions
 DMEM/F-12 Pedram, A., Razandi, M., Aitkenhead, M., and Levin, E: Estrogen Inhibits Cardiomyocyte Hypertrophy in vitro. Antagonism of Calcineurin-related Hypertrophy through Induction of MCIP1, J Biol Chem 280, 26339, 2005
Rat Rat, SD, 2-4 day Cardiomyocytes, fibroblasts Trypsin: 0.1%
Collagenase: 0.1%
 medium 199 Entcheva, E., Bien, H, Yin L., Chung, C., Farrell, M., and Kostov, Y.: Functional Cardiac Cell Constructs on Cellulose-based Scaffolding, Biomaterials 25(26), 5753, 2004
Rat Rat, SD, 1-2 day Cardiomyocytes NCIS kit: per instructions
 L-15 Muller-Bore, B., Cascio, W., Anderson, P., Snowwaert, J., Frye, J., Desai, N., Esch, G., Brackham, J., Bagnell, C., Coleman, W., Grisham, J., and Malouf, N.: Adult-derived Liver Stem Cells Acquire a Cardiomyocyte Structural and Functional Phenotype ex vivo, Am J Pathol 165, 135, 2004
Rat Rat,1-2 day Cardiomyocytes NCIS kit: per instructions
 DMEM Natarajan AR, Rong Q, Katchman AN, and Ebert SN.: Intrinsic cardiac catecholamines help maintain beating activity in neonatal rat cardiomyocyte cultures, Pediatr Res 56, 411, 2004
Rat Rat, 1 day Cardiomyocytes NCIS kit: per instructions
 F-10 Chen, Z., Ge, Y., and Kang J.: Down-regulation of the M6P/IGF-II Receptor Increases Cell Proliferation and Reduces Apoptosis in Neonatal Rat Cardiac Myocytes, BMC Cell Biol 5, 15, 2004
Rat Rat, neonatal Cardiac myocytes Collagenase Type 2: 0.5%
 HBSS Chen Hsiao-Huei, Mullett StevenJ, Stewart AlexandreF R: Vgl-4, a novel member of the vestigial-like family of transcription cofactors, regulates alpha1-adrenergic activation of gene expression in cardiac myocytes, J Biol Chem 279, 30800-6, 2004
Rat Rat, male, 250-300 g Cardiomyocytes Collagenase Type 1: 0.03%
Protease: 0.01%
 HBSS Hunton DaciaL, Zou LuYun, Pang Yi, Marchase Richard B: Adult rat cardiomyocytes exhibit capacitative calcium entry, Am J Physiol Heart Circ Physiol 286, H1124-32, 2004
Rat Rat, fetal or 3-day Cardiomyocytes Collagenase Type 2: 0.1%
 HEPES Tamamori-Adachi Mimi, Ito Hiroshi, Sumrejkanchanakij Piyamas, Adachi Susumu, Hiroe Michiaki, Shimizu Masato, Kawauchi Junya, Sunamori Makoto, Marumo Fumiaki, Kitajima Shigetaka, Ikeda Masa-Aki: Critical role of cyclin D1 nuclear import in cardiomyocyte proliferation, Circ Res 92, e12-9, 2003
Rat Rat, adult, 220-280g Cardiomyocytes Collagenase Type 2: 178 u/ml
Hyaluronidase: 0.01%
 Krebs-Henseleit calcium free Gordon JenniferM, Dusting GregoryJ, Woodman OwenL, Ritchie RebeccaH: Cardioprotective action of CRF peptide urocortin against simulated ischemia in adult rat cardiomyocytes, Am J Physiol Heart Circ Physiol 284, H330-6, 2003
Rat Rat, 50 g Ventricular myocytes Collagenase: 223 u/ml
Hyaluronidase: 0.01%
Trypsin: 0.002%
 Medium 199 Aberle II NS, and Ren J.: Experimental Assessment of the Role of Acetaldehyde in Alcoholic Cardiomyopathy, Biol Proced Online 5, 1, 2003
Rat Rat, neonatal Cardiomyocytes NCIS kit: per instructions
 L-15 Li TS, Ito H, Kajiwara K, and Hamano K.: Long-term survival of xenografted neonatal cardiomyocytes by adenovirus-mediated CTLA4-Ig expression and CD40 blockade, Circulation 108, 1760, 2003
Rat Rat, SD, 200-300 g Cardiomyocytes Collagenase Type 2: 0.05-0.1%
 Joklik's MEM Swift Luther, McHowat Jane, Sarvazyan Narine: Inhibition of membrane-associated calcium-independent phospholipase A2 as a potential culprit of anthracycline cardiotoxicity, Cancer Res 63, 5992-8, 2003
Rat Rat, SD, 175-200g Cardiomyocytes Collagenase Type 2: 0.05%
Deoxyribonuclease I: 0.02%
 Krebs-Ringer Tardif Annie, Julien Nathalie, Chiasson Jean-Louis, Coderre Lise: Stimulation of glucose uptake by chronic vanadate pretreatment in cardiomyocytes requires PI 3-kinase and p38 MAPK activation,Am J Physiol Endocrinol Metab Vol. 284,, , E1055-64, 2003
Rat Rat, SD, pregnant female Peritoneal mast Hyaluronidase: 100 u/ml
 DMEM (see reference) DeAlmeida, A., Mustin, D., Forman, M., Brower, G., Janicki, J., and Carver, W.: Effects of mast cells on the behavior of isolated heart fibroblasts: modulation of collagen remodeling and gene expression, J Cell Physiol 191, 51, 2002
Rat Rat, neonatal, adult, 17 day Ventricular cardiomyocytes Collagenase Type 2: 0.7-1%
 DMEM Lam ML, Bartoli M, and Claycomb WC.: The 21-day postnatal rat ventricular cardiac muscle cell in culture as an experimental model to study adult cardiomyocyte gene expression, Mol Cell Biochem 229, 51, 2002
Rat Rat, 1 day Cardiomyocytes NCIS kit: per instructions
 L-15 Yang Y, Liao H, Ke Q, Cai J, Xiao YF, and Morgan JP: Enhancement of nitric oxide production by methylecgonidine in cultured neonatal rat cardiomyocytes, Br J Pharmacol 135, 188, 2002
Rat Rat, female, 250-300 g Ventricular myocytes Collagenase Type 2: 0.1%
 Ringer solution Liu Qinghang, Hofmann Polly A: Antiadrenergic effects of adenosine A(1) receptor-mediated protein phosphatase 2a activation in the heart, Am J Physiol Heart Circ Physiol 283, H1314-21, 2002
Rat Rat, Wistar, 1 day Cardiomyocytes Collagenase Type 2: 80u/ml
 M199 Shimizu Tatsuya, Yamato Masayuki, Isoi Yuki, Akutsu Takumitsu, Setomaru Takeshi, Abe Kazuhiko, Kikuchi Akihiko, Umezu Mitsuo, Okano Teruo: Fabrication of pulsatile cardiac tissue grafts using a novel 3-dimensional cell sheet manipulation technique and temperature-responsive cell culture surfaces, Circ Res 90, e40, 2002
Rat Rat, male, 6-12 months old Cardiac myocytes Protease: 0.55 u/ml
 Tyrode's solution Kitta, K., Day, R., Ikeda, T., and Suzuki, Y.: Hepatocyte Growth Factor Protects Cardiac Myocytes Against Oxidative Stress-Induced Apoptosis, Free Radic Biol Med 31 (7), 902, 2001
Rat Rat, Harlan SD, pup Cardiomyocytes Trypsin:
DMEM/Ham's F-12 Schwartzbauer, G., and Robbins, J.: The Tumor Suppressor Gene PTEN Can Regulate Cardiac Hypertrophy and Survival, J Biol Chem 276, 35786, 2001
Rat Rat, SD, newborn Cardiac myocytes NCIS kit: per instructions
 L-15 Rahman A, Alam M, Rao S, Cai L, Clark LT, Shafiq S, and Siddiqui MA: Differential effects of doxorubicin on atrial natriuretic peptide expression in vivo and in vitro, Biol Res 34(3-4), 195, 2001
Rat Rat, adult Ventricular myocytes Collagenase: 223 u/ml
Hyaluronidase: 0.01%
Trypsin: 0.002%
 Tyrode solution Ren J, and Wold LE.: Measurement of Cardiac Mechanical Function in Isolated Ventricular Myocytes from Rats and Mice by Computerized Video-Based Imaging, Biol Proced Online 3, 43, 2001
Rat Rat, SD, male Cardiomyocytes Collagenase Type 2: 140 u/ml
 Krebs-Henseleit Dai L, Brookes PS, Darley-Usmar VM, and Anderson PG.: Bioenergetics in cardiac hypertrophy: mitochondrial respiration as a pathological target of NO*, Am J Physiol/Heart 281, H2261-H2269, 2001
Rat Rat, neonatal Cardiomyocytes NCIS kit: per instructions
 DMEM/M199 Guo K, Searfoss G, Krolikowski D, Pagnoni M, Franks C, Clark K, Yu KT, Jaye M, Ivashchenko Y: Hypoxia induces the expression of the pro-apoptotic gene BNIP3,Cell Death Differ Vol. 8,, , 367-76, 2001
Rat Rat, Wistar, female Ventricular myocytes Collagenase Type 2: 0.1%
 Ringer solution Pyle, W, Lester, J, and Hofmann, P.: Effects of Kappa-opioid receptor activation of myocardium, Am J Physiol Heart Circ Physiol 281, H669, 2001
Rat Rat, SD, 1-2 day Cardiomyocytes Trypsin: 0.01%
Collagenase Type 2: 0.08%
 HBSS Arutunyan A, Webster D, Swift L, Sarvazyan N: Localized injury in cardiomyocyte network: a new experimental model of ischemia-reperfusion arrhythmias, Am J Physiol Heart Circ Physiol 280, H1905-15, 2001
Rat Rat, newborn Atrial Trypsin: 0.06%
 DMEM Kim, D., and Pleumsamran, A.: Cytoplasmic Unsaturated Free Fatty Acids Inhibit ATP-Dependent Gating of the G Protein-gated K+ Channel, J Gen Physiol 115, 287, 2000
Rat Rat, 1-2 day Cardiomyocytes Collagenase Type 1: 84 u/ml
Pancreatin: 0.05%
 M199 medium Bueno OF, De Windt LJ, Tymitz KM, Witt SA, Kimball TR, Klevitsky R, Hewett TE, Jones SP, Lefer DJ, Peng CF, Kitsis RN, and Molkentin JD.: The MEK1-ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice, EMBO J 19(23), 6341, 2000
Rat Rat, Winstar, 1-2 day Ventricular myocytes Collagenase Type 2: 0.08%
 DMEM Maki T, Horio T, Yoshihara F, Suga S, Takeo S, Matsuo H, and Kangawa K: Effect of neutral endopeptidase inhibitor on endogenous atrial natriuretic peptide as a paracrine factor in cultured cardiac fibroblasts, Br J Pharmacol 131(6), 1204, 2000
Rat Rat, SD, adult female Heart microvascular cells CLSPA: 250u/ml
Papain: 5 u/ml
Elastase: 0.8 u/ml
 L-15 Puri S, Bansal DD, Uskokovic MR, and MacGregor RR.: Induction of tissue plasminogen activator secretion from rat heart microvascular cells by fM 1,25(OH)(2)D(3), Am J Physiol/Endo 278(2), E293, 2000
Rat Rat, SD, male, 250-300g Cardiomyocytes Collagenase Type 2: 0.06%
 M199 Sun L, Chang J, Kirchhoff SR, and Knowlton AA.: Activation of HSF and selective increase in heat-shock proteins by acute dexamethasone treatment, Am J Physiol Heart Circ Physiol 278(4), H1091, 2000
Rat Rat, wistar, 2 day Cardiomyocytes NCIS kit: per instructions
 DMEM-F12 Cowan DB, Poutias DN, Del Nido PJ, and McGowan FX Jr: CD14-independent activation of cardiomyocyte signal transduction by bacterial endotoxin, Am J Physiol Heart Circ Physiol 279, H619, 2000
Rat Rat, neonatal Cardiomyocytes NCIS kit: per instructions
 L-15 Webster DR, and Patrick DL.: Beating rate of isolated neonatal cardiomyocytes is regulated by the stable microtubule subset, Am J Physiol Heart Circ Physiol 278, H1653, 2000
Rat Rat, Lewis, adult male, 300-350-g Aorta, smooth muscle Trypsin: 0.025%
 Meduim 199 with 20% FBS medium Kim, B., Nikolovski, J., Bonadio, J., Smiley, E., and Mooney, D.: Engineered Smooth Muscle Tissues: Regulating Cell Phenotype with the Scaffold, Exp Cell Res 251, 318, 1999
Rat Rat, 10-15 newborn, 1-2 days old Atrial Trypsin: 0.03%
 HBSS or EBSS Nemec, J., Wickman, K., and Clapham, D.: GBy Binding Increases the Open Time of IKACh: Kinetic Evidence for Multiple GBy Binding Sites, Biophys J 76, 246, 1999
Rat Rat, 1-3 day Neonatal rat cardiomyocytes Collagenase Type 2: 80u/ml
Pancreatin: 0.06%
 DMEM/F12 Adderley SR, and Fitzgerald DJ.: Oxidative damage of cardiomyocytes is limited by extracellular regulated kinases 1/2-mediated induction of cyclooxygenase-2, J Biol Chem 274, 5038, 1999
Rat Rat, 1-2 day Cardiomyocytes Pancreatin: 0.06%
Collagenase Type 2: 95 u/ml
 DMEM Reinecke H, Zhang M, Bartosek T, and Murry CE.: Survival, integration, and differentiation of cardiomyocyte grafts: a study in normal and injured rat hearts, Circulation 100(2), 193, 1999
Rat Rat, adult and neonatal Cardioomyocytes Collagenase Type 4: 0.12%-adult
Collagenase Type 2: 0.05%-neonatal
 DMEM/medium 199 Richards SM, Jaconi ME, Vassort G, and Puceat M.: A spliced variant of AE1 gene encodes a truncated form of Band 3 in heart: the predominant anion exchanger in ventricular myocytes, J Cell Sci 112, 1519, 1999
Rat Rat, Wistar, male Cardiomyocytes Collagenase Type 2: 228 u/ml
 Joklik MEM Sambandam N, Abrahani MA, St Pierre E, Al-Atar O, Cam MC, and Rodrigues B.: Localization of lipoprotein lipase in the diabetic heart: regulation by acute changes in insulin, Arterioscler Thromb Vasc Biol 19(6), 1526, 1999
Rat Rat, 1-2 day Cardiomyocytes NCIS kit: per instructions
 RPMI Matsui, T., Li, Ling, Del Monte, F., Fukui, Y., Franke, T., Hajjar, R., Rosenzweig, A.: Adenoviral gene transfer of activated phosphatidylinositol 3'-kinase and Akt inhibits apoptosis of hypoxic cardiomyocytes in vitro, Circulation 100, 2373-9, 1999
Rat Rat Thoracic aorta, smooth muscle Elastase: 0.02%
 (see reference) Zilberman, A., Dave, V., Miano, J., Olson, E., and Periasamy, M.: Evolutionary Conserved Promoter Region Containing CArg-Like Elements is Crucial for Smooth Muscle Myosin Heavy Chain Gene Expression, Circ Res 82, 566, 1998
Rat Rat, SD, 1 day Cardiomyocytes NCIS kit:
DMEM/F-12 Wagner DR, Combes A, McTiernan C, Sanders VJ, Lemster B, Feldman AM: Adenosine inhibits lipopolysaccharide-induced cardiac expression of tumor necrosis factor-alpha, Circ Res 82, 47-56, 1998
Rat Rat, SD, 300-350 g Cardiomyocytes Collagenase Type 2: 0.04%
 see reference Yu L, Netticadan T, Xu YJ, Panagia V, Dhalla NS: Mechanisms of lysophosphatidylcholine-induced increase in intracellular calcium in rat cardiomyocytes, J Pharmacol Exp Ther 286, 1-8, 1998
Rat Rat, SD, female, 200g Ventricular cardiac myocytes Collagenase Type 2: 0.5%
Hyaluronidase: 0.2%
 Krebs-Henseleit buffer Westfall M V, Rust E M, Metzger J M: Slow skeletal troponin I gene transfer, expression, and myofilament incorporation enhances adult cardiac myocyte contractile function, Proc Natl Acad Sci U S A 94, 5444-9, 1997
Rat Rat, Wistar, 1 day Cardiomyoctes Collagenase Type 2: 80 u/ml
 HBSS Kinugawa, K., Kohmoto, O., Yao, A., Serizawa, T., and Takahashki, T.: Cardiac inducible nitric oxide synthase negatively modulates myocardial function in cultured rat myocytes, Am J Physiol 272, H35-47, 1997
Rat Rat, neonatal, 5-day Cardiomyocytes Trypsin: 0.05%
 DMEM Vanwinkle, W., Snuggs, M., and Buja, L.: Cardiogel: A Biosynthetic Extracellular Matrix for Cardiomyocyte Culture, In Vitro Cell Dev Biol Anim 32, 478, 1996
Rat Rat, SD, 250-300 g Cardiomyocytes Collagenase Type 2: 0.05%
 Joklik's Sharma VK, Colecraft HM, Wang DX, Levey AI, Grigorenko EV, Yeh HH, and Sheu SS: Molecular and functional identification of m1 muscarinic acetylcholine receptors in rat ventricular myocytes, Circ Res 79(1), 86, 1996
Rat Rat, SD, fetal and neonatal Cardiomyocytes Collagenase: 0.1%
Trypsin: 0.2%
 PBS Li RK, Mickle DA, Weisel RD, Zhang J, Mohabeer MK: In vivo survival and function of transplanted rat cardiomyocytes, Circ Res 78, 283-8, 1996
Rat Rat, SD, male, 200-250 g Myocytes Collagenase: 0.07%
 Joklik's MEM with HEPES Laughlin, M.H., Schaefer, M.E., and Sturek, M.: Effect of Exercise Training on Intracellular Free Ca2+ Transients in Ventricular Myocytes of Rats, J Appl Physiol 73, 1441, 1992
Rat Rat, SD, male, 200-250 g Myocytes Ventricles Collagenase: 0.07%
 Joklik's MEM Laughlin, M., Schaeffer, M., and Sturek, M.: Effect of Exercise Training on Intracellular Free Ca2+ Transients in Ventricular Myocytes of Rats, Am J Physiol 73, 1141, 1992
Rat Rat, SD, female, 60-90 days Myocardial Collagenase Type 2: 0.1%
 Joklik's MEM, Kreb's-Henseleit buffer, CF Welder, A.A., Grant, R., Bradlaw, J., and Acosta, D.: A Primary Culture System of Adult Rat Heart Cells for the Study of Toxicologic Agents, In Vitro Cell Dev Biol 27, 921, 1991
Rat Rat, SD, adult, female, 180-220 g Cardiomyocytes Collagenase: 0.11%
 (see reference) Fischer, Y., Rose, H., and Kammermeier. H.: Highly Insulin-Responsive Isolated Rat Heart Muscle Cells Yielded By a Modified Isolation Method, Life Sci 49, 1679, 1991
Rat Rat, neonatal, 1-4 days Cardiomyocytes Collagenase Type 2: 0.12%
 Krebs-Henseleit Buffer, CF Marino, T.A., Walter, R.,Cobb, E., Palasiuk, M., Parsons, T., and Mercer, W.E.: Effects of Norepinephrine on Neonatal Rat Cardiocyte Growth and Differentiation, In Vitro Cell Dev Biol 26, 229, 1990
Rat Rat
(also bovine)		 Heart Adrenal chromaffin Paraneurons Trypsin: 0.06%
 25mM HEPES buffered Locke's solution, CMF Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990
Rat Rat, adult, female Myocytes Collagenase Type 2: 0.05%
 Joklik's MEM Nag, A.C., Lee, M., and Kosiur, J.R.: Adult Cardiac Muscle Cells in Long-Term Serum-Free Culture:Myofibrillar Organization and Expression of Myosin Heavy Chain Isoforms, In Vitro Cell Dev Biol 26, 464, 1990
Rat Rats, AW, late-gestation pregnant females Ventricular Cardiomyocytes Collagenase Type 2: 0.05 - 0.08%
 See reference Engelmann, G., McTiernan, C., Gerrity, R., and Samarel, A.: Technique 2 (6), 279, 1990
Rat Rat,SD, albino, 10-14 weeks Myocytes Collagenase Type 1: 100 u/ml and 150 u/ml
 Krebs Ringer bicarbonate buffer Berg, I., Guse, A.H., and Gercken, G.: Carbamoylcholine-Induced Accumulation of Inositol Mono-,Bis-,Tris-, And Tetrakisphosphates In Isolated Cardiac Myocytes From Adult Rats, Biochim Biophys Acta 1010, 100, 1989
Rat Rat, SD, adult, female, 175 - 225 g Myocytes Collagenase: 0.1%
 Joklik's medium Bugaisky, L.B. and Zak, R.: Differentiation of Adult Rat Cardiac Myocytes in Cell Culture, Circ Res 64, 493, 1989
Rat Rat, SD, male, 250 - 350 g Myocytes, ventricular Collagenase Type 1: 90 and 100 u/ml
 Joklik's solution with and without calcium De Young, M., Giannattasio, B., and Scarpa, A.: Isolation of Calcium-Tolerant Atrial And Ventricular Myocytes From Adult Rat Heart, Methods Enzymol 173, 662, 1989
Rat Rat, SD, 2-4 day old Cardiomyocytes Fibroblasts Trypsin: 0.1%
 HBSS Toraason, M., Luken, M.E., Breitenstein, M., Krueger, J.A., and Biagini, R.E.: Comparative Toxicity of Allylamine and Acrolein in Cultured Myocytes and Fibroblasts from Neonatal Rat Heart, Toxicology 56, 107, 1989
Rat Rat, SD, male Aortic smooth muscle Trypsin: 0.05%
 DMEM Cornwell, T., and Lincoln, T.: Regulation of Intracellular Ca2+ Levels in Cultured Vascular Smooth Muscle Cells, J Biol Chem 264 (2), 1146, 1989
Rat Rat, adult, 150-200 g Myocytes, atria Collagenase Type 1: 100 u/ml
 Kreb's Ringer bicarbonate - HEPES buffer McMahon, K.K.: A Study of Adult Rat Atrial Myocyte Attachment to Extricellular Matrix Components and Long Term Culture,Biology of Isolated Adult Cardiac Myocytes,(Clark, Decker, Borg, Eds), Elsevier Science Publishing Co. Inc., , 1988
Rat Rat, SD Myocytes Collagenase Type 2: 0.1%
 Kreb's Ringer w/ calcium Buxton, I.L.O. and Doggwiler, K.O.: Alpha1-Adrenergic Receptor Signal Transduction in the Adult Rat Cardiac Myocyte,Biology of Isolated Adult Cardiac Myocytes,(Clark, Decker, Borg, eds), , 298, 1988
Rat Rat, SD, male, 250-350 g Myocytes, heart Hyaluronidase: 0.03%
 Bicarbonate-buffered medium Kim, D. and Smith, T.W.: Temporal Variation in Contractile State And [Ca++] in Isolated Adult Rat And Guinea Pig Cardiac Myocytes,Biology of Isolated Adult Cardiac Myocytes,(Clark, Decker, Borg, eds), , 370, 1988
Rat Rat, Wistar, 2-4 day neonates Myoblast, cardiac Trypsin: 0.1%
 Standardized Medium
(see reference)		 Grynberg, A., Degois, M., Guenot, L., and Athias, P.: Primary Rat Cardiac Cell Culture: Diet of the Mother Rats as a Determinant Parameter of Cardiomyoblast Production from Neonates, Biol Cell 57, 89, 1986
Rat Rat, SD, timid-pregnant Myocytes Trypsin: 2.4 u/ml
 DMEM Freerksen, D., Schroedl, N., and Hartzell, C.: Control of Enzyme Activity Levels by Serum and Hydrocortisone in Neonatal Rat Heart Cells Cultured in Serum-Free Medium, J Cell Physiol 120, 126, 1984
Rat Rat, SD, female, 12 weeks Myocytes Collagenase Type 1: 0.06%
 Krebs Ringer bicarbonate buffer Piper, H.M., Probst, I., Schwartz, P., Hutter, F.J., and Spieckermann, P.G.: Culturing of Calcium Stable Adult Cardiac Myocytes, J Mol Cell Cardiol 14, 397, 1982
Rat Rat, male/female, 200-250 g Muscle Hyaluronidase: 0.1%
 Krebs Ringer phosphate buffer, CMF Nag, A. and Cheng, M.: Adult Mammalian Cardiac Muscle Cells in Culture, Tissue Cell 13, 515, 1981
Rat Rat, female, adult, 200 - 300 g Myocytes Collagenase Type 2: 0.1%
 Joklik's MEM Claycomb, W.C., and Palazzo, M.C.: Culture of The Terminally Differentiated Adult Cardiac Muscle Cell: A Light and Scanning Electron Microscope Study, Dev Biol 80, 466, 1980
Rat Rat, SD, adult, male, 200-250 g Myocytes Hyaluronidase: 0.10%
 Joklik MEM, CF Frangakis, C., Bahl, J., McDaniel, H., and Bressler, R.: Tolerance to Physiological Calcium by Isolated Mycocytes from the Adult Rat Heart; An Improved Cellular Preparation, Life Sci 27, 815, 1980
Rat Rat, SD, female, 12 wks, 250 g Myocytes Ventricular myocardium Collagenase Type 1: 0.20%
 Kreb's Ringer bicarbonate buffer Powell, T., Terrar, D.A., and Twist, V.W.: Electrical Properties of Individual Cells Isolated From Adult Rat Ventricular Myocardium, J Physiol 302, 131, 1980
Rat Rat, 10 wk Myocytes Collagenase Type 1: 0.1%
 Saline Bishop, S., Oparil, S., Reynolds, R., and Drummond, J.: Regional Myocyte Size in Normotensive and Spontaneously Hypertensive Rats, Hypertension 1 (4), 378, 1979
Rat Rat, male, 200-300 g Myocytes Hyaluronidase: 82 u/ml
 Kreb's Henseleit buffer Farmer, B., Harris, R., Jolly, W., Hathaway, D., Katzberg, A., Watanabe, A., Whitlow, A., and Besch, H.: Isolation and Characterization of Adult Rat Heart Cells, Arch Biochem Biophys 179, 545, 1977
Rat Rat, female, albino, 200-250 g Myocytes Hyaluronidase: 0.1%
 Kreb's Ringers phosphate Nag, A., Fischman, D., Aumont, M., and Zak, R.: Studies of Isolated Adult Rat Heart Cells: The Surface Morphology and the Influence of Extracellular Calcium Ion Concentration on Cellular Viability, Tissue Cell 9 (3), 419, 1977
Rat Rat, adult Myocytes Collagenase: 0.1%
 Perfusing solution Moustafa, E., Skomedal, T., Osnes, J., and Oye, I.: Cyclic Amp Formation and Morphology of Myocardial Cells Isolated from Adult Heart: Effect of Ca2+ and Mg2+, Biochim Biophys Acta 421, 411, 1976
Rat Rat, SD, albino, male, 180-230 g Heart ventricles, beating Collagenase: 0.05-0.2%
 Phosphate buffer Glick, M., Burns, A., and Reddy, W.: Dispersion and Isolation of Beating Cells from Adult Rat Heart, Anal Biochem 61, 32, 1974
Rat Rat, Hebrew Un strain, 3 & 17 months, 150-210 g Myocytes Trypsin: 0.05%
 Versene buffer Bierman, E.L., Stein, O., and Stein, Y.: Lipoprotein Uptake and Metabolism by Rat Aortic Smooth Muscle Cells in Tissue Culture, Circ Res 35, 136, 1974
Rat Rat, 2-5 day Myocytes Trypsin: 0.01%
 Saline A Speicher, D.W., and McCarl, R.L.: Pancreatic Enzyme Requirements for the Dissociation of Rat Hearts for Culture, In Vitro 10, 30, 1974
Rat Rat, SD, male, 150-200 g Myocytes Hyaluronidase: 0.05%
 (see reference) Pretlow II, T., Glick, M., and Reddy, W.: Separation of Beating Cardiac Myocytes from Suspensions of Heart Cells, Am J Pathol 67 (2), 215, 1972
Rat Rat, albino, male, 250 g Myocytes Hyaluronidase: 0.20%
 Hank's solution, CF Berry, M., Friend, D., and Scheuer, J.: Morphology and Metabolism of Intact Muscle Cells Isolated from Adult Rat Heart, Circ Res 26, 679, 1970
Rat Rat, adult, male, 250 g Myocytes Trypsin: 0.1%
 Saline A Vahouny, G.V., Wei, R., Starkweather, R., and Davis, C.: Preparation of Beating Heart Cells From Adult Rats, Science 167, 1616, 1970
Rat Rat, Wistar, 1-4 day old Myocytes Trypsin: 0.125%
 Gey's BSS Mark, G., and Strasser, F.: Pacemaker Activity and Mitosis in Cultures of Newborn Rat Heart Ventricle Cells, Exp Cell Res 44, 217, 1966
Shellfish Pacific Oysters Haemocytes Trypsin:
L15 medium Le Duff, R., Lipart, C., and Renault, T.: Primary Culture of Pacific Oyster, Crassostrea gigas, heart cells, J Tiss Cul Meth 16, 67, 1994
Shellfish Clams, 3.0 to 4.5 cm Myocytes Fibroblasts Collagenase Type 1: 2%
 L15 medium Wen, C., Kou, G., and Chen, S.: Cultivation of Cells From the Heart of the Hard Clam, Merretrix lusoria (Roding), J Tiss Cul Meth 15, 123, 1993