Intestine - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Canine Dog, adult Intestinal L-cells Collagenase Type 1: 75 u/ml
 HBSS Damholt AB, Buchan AM, Kofod H: Glucagon-like-peptide-1 secretion from canine L-cells is increased by glucose-dependent-insulinotropic peptide but unaffected by glucose, Endocrinology 139, 2085-91, 1998
Human Human Lamina propria mononuclear cells Collagenase: 0.02%
 HBSS Kanai Takanori, Totsuka Teruji, Uraushihara Koji, Makita Shin, Nakamura Tetsuya, Koganei Kazutaka, Fukushima Tsuneo, Akiba Hisaya, Yagita Hideo, Okumura Ko, Machida Utako, Iwai Hideyuki, Azuma Miyuki, Chen Lieping, Watanabe Mamoru: Blockade of B7-H1 suppresses the development of chronic intestinal inflammation, J Immunol 171, 4156-63, 2003
Human Human Mucosal mononuclear cells Collagenase Type 3: 0.01%
Deoxyribonuclease I: 0.01%
Soybean Trypsin Inhibitor: 0.01%
 RPMI Stallmach A, Schaffer F, Hoffmann S, Weber S, Muller-Molaian I, Schneider T, Kohne G, Ecker KW, Feifel G, Zeitz M: Increased state of activation of CD4 positive T cells and elevated interferon gamma production in pouchitis, Gut 43, 499-505, 1998
Mouse Mouse, 7 week Interstitial cells Collagenase: 0.04%
Trypsin: 0.02%
 see reference Goto Kazunori, Matsuoka Satoshi, Noma Akinori: Two types of spontaneous depolarizations in the interstitial cells freshly prepared from the murine small intestine, J Physiol 559, 411-22, 2004
Mouse Mouse, 9-13 day Interstitial cells of Cajal Collagenase Type 2: 0.13%
 HBSS Ordag Tamas, Redelman Doug, Miller Lisa J, Horvath Viktor J, Zhong Qiao, Almeida-Porada Graca, Zanjani Esmail D, Horowitz Burton, Sanders Kenton M: Purification of interstitial cells of Cajal by fluorescence-activated cell sorting, Am J Physiol Cell Physiol 286, C448-56, 2004
Mouse Mouse, 0-15 day Interstitial cells of Cajal Collagenase Type 2: 0.13%
 Hanks Lee Young Mee, Kim Byung Joo, Kim Hyun Jin, Yang Dong Ki, Zhu Mei Hong, Lee Kyu Pil, So Insuk, Kim Ki Whan: TRPC5 as a candidate for the nonselective cation channel activated by muscarinic stimulation in murine stomach,Am J Physiol Gastrointest Liver Physiol Vol. 284,, , G604-16, 2003
Mouse Mouse Lamina propria lymphocytes Collagenase Type 4: 300 u/ml
 PBS Wu Y, Wang X, Csencsits KL, Haddad A, Walters N, Pascual DW: M cell-targeted DNA vaccination, Proc Natl Acad Sci U S A 98, 9318-23, 2001
Mouse Mouse, F14 Intestinal mesenchymal Collagenase Type 2: 0.03%
 HBSS Sakagami Y, Inaguma Y, Sakakura T, Nishizuka Y: Intestine-like remodeling of adult mouse glandular stomach by implanting of fetal intestinal mesenchyme, Cancer Res 44, 5845-9, 1984
Rat Rat, male, 18-24 day Intestinal epithelial Collagenase Type 1: 0.1%
 DMEM Quaroni A, Wands J, Trelstad RL, Isselbacher KJ: Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria, J Cell Biol 80, 248-65, 1979