Liver - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Avian Ducklings, 7 day Hepatocytes Collagenase: 0.05%
Hyaluronidase: 0.05%
 DMEM/ F12 Lee J., Culvenor J., Angus P., Smallwood R., Nicoll A., and Locarnini S.: Duck Hepatitis B Virus Replication in Primary Bile Duct Epithelial Cells, J Virol 75(16), 7651, 2001
Canine Dog, 13 years old
(also rat, guinea-pig, rabbit, human)		 Hepatocytes Collagenase: 90 u/ml
 CF EGTA perfusate Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies, In Vitro 17, 935, 1981
Canine Dog, adult Hepatocytes Trypsin: 0.1%
 HBSS, CMF Vickrey, H., Ramon, J., and McCann, D.: Continuous Culture of Normal Adult Mammalian Hepatocytes Exhibiting Parenchymal Functions, In Vitro 15, 120, 1979
Chicken Chicken, adult Hepatocytes Collagenase: 0.02%
 HEPES, CF Fraslin, J., Touquette, L., Douaire, M., Menezo, Y., Guillemot, J., and Mallard, J.: Isolation and Long Term Maintenance of Differentiated Adult Chicken Hepatocytes in Primary Culture, In Vitro Cell Dev Biol 28, 615, 1992
Chicken Chicken
(also rat, SD, 150-250 g)		 Hepatocytes Collagenase Type 4: 6000 units
 Medium A Roseman, S., Weigel, P., Schnaar, R., Kuhlenschmidt, M., Schmell, E., Lee, R., and Lee, Y.: Adhesion of Hepatocytes to Immobilized Sugars. A Threshold Phenomenon, J Cell Biol 254, 10830, 1979
Chicken Chicken, Leghorn, white, 10-15 day Hepatocytes Deoxyribonuclease I: 0.00125%
 PBS Tarlow, D., Watkins, P., Reed, R., Miller, R., Zwergel, E., and Lane, M.: Lipogenesis and the Synthesis and Secretion of Very Low Density Lipoprotein by Avian Liver Cells in Nonproliferating Monolayer Culture, J Cell Biol 73, 332, 1974
Chicken Chick embryos, 5 day Heart Liver Trypsin: 3.0%
 Tyrode's solution, CMF Steinberg, M.: "ECM": Its Nature, Origin, And Function in Cell Aggregation, Exp Cell Res 30, 257, 1963
Chicken Chick embryonic Various tissues (heart, liver, skeletal, cardiac) Trypsin: various grades
 CMF Tyrode's solution Rinaldini, L.: An Improved Method for the Isolation and Quantitative Cultivation of Embryonic Cells, Exp Cell Res 16, 477, 1959
Equine Equine, 4-13 yr Hepatocytes Collagenase Type 4: 0.1%
 HBSS Bakala A., Karlik W., and Wiechetek M.: Preparation of Equine Isolated Hepatocytes, Toxicol In Vitro 17(5-6), 615, 2003
Fish Rainbow trout (Oncorhynchus mykiss), male & female,
120-600 g		 Hepatocytes Collagenase Type 2: 0.045%
 Kreb's-Ringer bicarbonate buffer, CF Blair, J.B., Miller, M.R., Pack, D., Barnes, R., Teh, S.J. and Hinton, D.E.: Isolated Trout Liver Cells:Establishing Short-Term Primary Cultures Exhibiting Cell-to-Cell Interactions, In Vitro Cell Dev Biol 26, 237, 1990
Fish Trout, male, 100 - 200 g Hepatocytes Collagenase Type 2: 0.045%
 HBSS with 0.05M HEPES Lipsky, M., Sheridan, T., Bennett, R., and May, E.: Comparison of Trout Hepatocyte Culture on Different Substrates, In Vitro Cell Dev Biol 22, 360, 1986
Fish Rainbow trout, (Salmo gairdneri), male, 150-200 g Hepatocytes Collagenase: 100 u/ml
 HBSS/CMF Klaunig, J., Ruch, R., and Goldblah, P.: Trout Hepatocyte Culture: Isolation and Primary Culture, In Vitro Cell Dev Biol 21, 221, 1985
Fish Trout 100g - 5kg Hepatocytes Hyaluronidase: 0.08%
 See reference Bailey, G., Taylor, M., and Selivonchick, D.: Aflatoxin B1 Metabolism and DNA Binding in Isolated Hepatocytes From Rainbow Trout Salmo gairdner, Carcinogenesis 3, 511, 1982
Fish Trout (Salmo gairdneri), male, 150-200 g
Catfish (Ictalurus punctatus), male, 100 - 150 g		 Hepatocytes Collagenase: 100u/ml
 HBSS Klaunig, J.: Establishment of Fish Hepatocyte Cultures For Use in In Vitro Carcinogenicity Studies, Natl Cancer Inst Monogr 65, 163, 1981
Guinea-Pig Guinea-pig, Hartley, male, 250-300 g Hepatocytes Collagenase Type 2:
Kreb's Ringer bicarbonate buffer Arinze, I. and Kawai, Y.: Adrenergic Regulation of Glycogenolysis in Isolated Guinea-Pig Hepatocytes: Evidence that B2-Receptors Mediate Catecholamine Stimulation of Glycogenolysis, Arch Biochem Biophys 225, 196, 1983
Guinea-Pig Guinea-pig, young Hepatocytes Collagenase: 90 u/ml
 CF EGTA perfusate Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies, In Vitro 17, 935, 1981
Human Human Hepatocytes Collagenase: 0.05%
 HEPES Pichard L, Raulet E, Fabre G, Ferrini JB, Ourlin JC, and Maurel P: Human hepatocyte culture, Methods Mol Biol 320, 283, 2006
Human Human Hepatocytes Collagenase: 0.05%
 EBSS Hughes, R., Mitry, R., Dhawan, A., Lehec, S., Girlanda, R., Rela, M., Heaton, N., and Muiesan, P.: Isolation of Hepatocytes from Livers from Non-Heart-Beating Donors for Cell Transplantation, Liver Transpl 12, 713, 2006
Human Human, adult Hepatocytes Collagenase: 0.6%
 RPMI 1640 Cho JJ, Joseph B, Sappal BS, Giri RK, Wang R, Ludlow JW, Furth ME, Susick R, and Gupta S.:   Analysis of the functional integrity of cryopreserved human liver cells including xenografting in immunodeficient mice to address suitability for clinical applications, Liver Int 24, 361, 2004
Human Human Hepatocytes Collagenase Type 4: 0.05%
 Williams medium E Duanmu Z., Locke D., Smigelski J., Wu W., Dahn M., Falany C., Kocarek T., and Runge-Morris M.: Effects of Dexamethasone on Aryl (SULT1A1)- and hydroxysteroid (SULT2A1)-Sulfotransferase Gene Expression in Primary Cultured Human Hepatocytes, Drug Metab Dispos 30(9), 997, 2002
Human Human
(also rat)		 Hepatocytes Collagenase Type 1: 0.05%
 Leffert's buffer Dandri, M., Burda, M., Torok, E., Pollok, J., Iwanska, A., Sommer, G., et al.: Repopulation of Mouse Liver with Human Hepatocytes and In Vivo Infection with Hepatitis B Virus, Hepatol 33, 981, 2001
Human Human Hepatocytes Collagenase: 0.05%
 Williams E Donato M, Viitala P, Rodriguez-Antona C, Lindfors A, Castell J, Raunio H, Gomez-Lechon M, Pelkonen O: CYP2A5/CYP2A6 expression in mouse and human hepatocytes treated with various in vivo inducers, Drug Metab Dispos 28, 1321-6, 2000
Human Human Hepatocytes Collagenase: 0.05%
 HEPES buffer
(see reference)		 Gomez-Lechon, M., Lopez, P., Donato, T., Montoya, A., Larrauri, A., Gimenez, P., Trullenque, R., Fabra, R., and Castell, J.: Culture of Human Hepatocytes From Small Surgical Liver Biopsies: Biochemical Characterization And Comparison With in vivo, In Vitro Cell Dev Biol 26, 67, 1990
Human Human, male, 17-40 yrs. Hepatocytes Collagenase: 0.05%
 HEPES buffer Begue, J., Baffet, G., Campion, J., and Guillouzo, A.: Differential Response of Primary Cultures of Human and Rat Hepatocytes to Aflatoxin B1-Induced Cytotoxicity and Protection by the Hepatoprotective agent(+)-Cyanidanol-3, Biol Cell 63, 327, 1988
Human Human, 4 male kidney donors, age between 17 and 31
(also adult rats, male, SD, 180-200 g)		 Hepatocytes Collagenase: 0.05% & 0.025%
 HEPES
(see reference)		 LeBot, M., Begue, J., Kernaleguen, D., Robert, J., Ratanasavanh, D., Airiau, J., Riche, C., and Guillouzo, A.: Different Cytotoxicity and Metabolism of Doxorubicin, Daunorubicin, Epirubicin, Esorubicin, and Idarubicin in Cultured Human and Rat Hepatocytes, Biochem Pharmacol 37 (20), 3877, 1988
Human Human, 3 human kidney donors, age 20, 23, 25 Hepatocytes Collagenase: 0.05%
 HEPES Gugen-Guillouzo, C., Campion, J., Brissot, P., Glaise, D., Launois, B., Bourel, M., and Guillouzo, A.: High Yield Preparation of Isolated Human Adult Hepatocytes by Enzymatic Perfusion of the Liver, Cell Biol Int Rep 6 (6), 625, 1982
Human Human, 51-75 years old
(also rat, guinea-pig, rabbit, dog)		 Hepatocytes Collagenase: 90 u/ml
 CF EGTA perfusate Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies, In Vitro 17, 935, 1981
Human Human Hepatocytes Trypsin: 0.1%
 HBSS, CMF Kaighn, M.: Human Liver Cells,Tissue Culture Methods / Applications,(Kruse, P., Patterson, M. eds), , 54, 1973
Monkey Monkey (Macaca fascicularis), adult, 5-6 Kg Hepatocytes Trypsin: 160 u/ml
 HEPES buffer Ulrich, R., Aspar, D., Cramer, C., Kletzien, R., and Ginsberg, L.: Isolation and Culture of Hepatocytes from the Cynogmolgus Monkey (Macaca fascicularis), In Vitro Cell Dev Biol 26, 815, 1990
Mouse Mouse Liver epithelial progenitor cells Collagenase Type 4: 0.1%
Deoxyribonuclease I: 0.05%
 DMEM Li Wen-Lin, Su Juan, Yao Yu-Cheng, Tao Xin-Rong, Yan Yong-Bi, Yu Hong-Yu, Wang Xin-Min, Li Jian-Xiu, Yang Yong-Ji, Lau Joseph T Y, Hu Yi-Ping: Isolation and characterization of bipotent liver progenitor cells from adult mouse, Stem Cells 24, 322-32, 2006
Mouse Mouse Liver sinusodial endothelial Collagenase: 0.03%
 DMEM Benten Daniel, Follenzi Antonia, Bhargava KuldeepK, Kumaran Vinay, Palestro ChristopherJ, Gupta Sanjeev: Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice, Hepatol 42, 140-8, 2005
Mouse Mouse Hepatocytes Collagenase Type 1: 0.033%
 Leffert's buffer Jiang Guoqiang, Li Zhihua, Liu Franklin, Ellsworth Kenneth, Dallas-Yang Qing, Wu Margaret, Ronan John, Esau Christine, Murphy Cain, Szalkowski Deborah, Bergeron Raynald, Doebber Thomas, Zhang Bei: Prevention of obesity in mice by antisense oligonucleotide inhibitors of stearoyl-CoA desaturase-1, J Clin Invest 115, 1030-8, 2005
Mouse Mouse Hepatocytes Collagenase Type 1: 0.053%
 DMEM/ F-12 Sazani P., Gemignani F., Kang S., Maier M., Manoharan M., Persmark M., Bortner D., and Kole R.: Systemically Delivered Antisense Oligomers Up-regulate Gene Expression in Mouse Tissues, Nat Biotechnol 20, 1228, 2002
Mouse Mouse, male, 4-10 week Hepatocytes Collagenase Type 2: 100 u/ml
 HBSS Lingohr Melissa K, Bull Richard J, Kato-Weinstein Junko, Thrall Brian D: Dichloroacetate stimulates glycogen accumulation in primary hepatocytes through an insulin-independent mechanism,Toxicol Sci Vol. 68,, , 508-15, 2002
Mouse Mouse Hepatocytes Collagenase: 0.05%
 Williams E Donato M, Viitala P, Rodriguez-Antona C, Lindfors A, Castell J, Raunio H, Gomez-Lechon M, Pelkonen O: CYP2A5/CYP2A6 expression in mouse and human hepatocytes treated with various in vivo inducers, Drug Metab Dispos 28, 1321-6, 2000
Mouse Mouse, 7 week Kupffer cells Collagenase Type 4: 0.05%
 HBSS Angele MK, Knoferl MW, Schwacha MG, Ayala A, Cioffi WG, Bland KI, and Chaudry IH.: Sex steroids regulate pro- and anti-inflammatory cytokine release by macrophages after trauma-hemorrhage, Am J Physiol 277, C35, 1999
Mouse Mouse Nonparenchymal liver Collagenase Type 1: 0.05%
 Hanks Ling W, Lougheed M, Suzuki H, Buchan A, Kodama T, Steinbrecher UP: Oxidized or acetylated low density lipoproteins are rapidly cleared by the liver in mice with disruption of the scavenger receptor class A type I/II gene, J Clin Invest 100, 244-52, 1997
Mouse Mouse, C3H, 6-8 weeks Epithelial Collagenase: 0.10%
 DMEM Lillehaug, J., Mondal, S., and Heidelberger, C.: Establishment of Epithelial Cell Lines from Mouse Regenerating Liver, In Vitro 15, 910, 1979
Mouse Mouse, 20-30 g Parenchymal and non-parenchymal Hyaluronidase: 0.1%
 Hank's w/ Insulin, CMF Crisp, D., and Pogson, C.: Glycolytic and Gluconeogenic Enzyme Activities in Parenchymal and Non-parenchymal Cells from Mouse Liver, Biochem J 126, 1009, 1972
Porcine Piglets, one week Hepatocytes Collagenase Type 4: 0.05%
 RPMI 1640 Wang Y., Liu H., Guo H., Wen H., and Liu J.: Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich, World J Gastroenterol 10, 699, 2004
Porcine Porcine, male, 2-3 week Hepatocytes Collagenase: 0.05%
 DMEM Raman Priya, Donkin Shawn S, Spurlock Michael E: Regulation of hepatic glucose metabolism by leptin in pig and rat primary hepatocyte cultures,Am J Physiol Regul Integr Comp Physiol Vol. 286,, , R206-16, 2004
Porcine Pig, adult Hepatocytes Collagenase: 0.05%
Dispase:
MEM Zhou X, Liu L, Kano J, Mukaiyama T, and Tokiwa T.: Isolation and Cultivation of Porcine Hepatocytes for Extracorporeal Artificial Liver Support System, Chin Med J 114, 946, 2001
Porcine Porcine, male, 6-40 kg Hepatocytes Collagenase: 0.8%
 PBS Gerlach, J., Brombacher, J., Smith, M., Neuhaus, P.: High Yield Hepatocyte Isolation from Pig Livers for Investigation of Hybrid Liver Support Systems: Influence of Collagenase Concentration and Body Weight, J Surg Res 62 (1), 85, 1996
Rabbit Rabbit, New Zealand white Hepatocytes Collagenase: 90 u/ml
 CF EGTA perfusate Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies, In Vitro 17, 935, 1981
Rat Rat, Fisher, E14 Hepatocytes Collagenase Type 1: 0.22%
 HBSS Isabel Zvibel, Miri Bronstein, Einav Hubel, Ella Bar-Lev, Zamir Halpern, Ran Oren: Isolation, characterization and culture of Thy1-positive cells from fetal rat livers, World J Gastroenterol 12, 3841-7, 2006
Rat Rat, Lewis, female, 150-200 g Hepatocytes Collagenase: see references
 DMEM Sosef MN, Baust JM, Sugimachi K, Fowler A, Tompkins RG, and Toner M.: Cryopreservation of isolated primary rat hepatocytes: enhanced survival and long-term hepatospecific function, Ann Surg 241, 125, 2005
Rat Rat Liver sinusodial endothelial Collagenase: 0.03%
 DMEM Benten Daniel, Follenzi Antonia, Bhargava KuldeepK, Kumaran Vinay, Palestro ChristopherJ, Gupta Sanjeev: Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice, Hepatol 42, 140-8, 2005
Rat Rat, Wistar, 200-300 g Hepatocytes Collagenase Type 2: 0.05%
 Williams E Pütz G, Schmider W, Nitschke R, Kurz G, Blum HE: Synthesis of phospholipid-conjugated bile salts and interaction of bile salt-coated liposomes with cultured hepatocytes, J Lipid Res 46, 2325-38, 2005
Rat Rat, adult Hepatocytes Collagenase Type 1: 0.1%
Pronase: 1%
Deoxyribonuclease I: 0.007%
 Williams E medium Jensen C., Jauho E., Santoni-Rugiu E., Holmskov U., Teisner B., Tygstrup N., and Bisgaard H.: Transit-amplifying Ductular (oval) Cells and Their Hepatocytic Progeny are Characterized by a Novel and Distinctive Expression of Delta-like Protein/preadipocyte Factor 1/fetal Antigen 1, Am J Pathol 164(4), 1347, 2004
Rat Rat, wistar Hepatocytes Collagenase Type 4: 0.05%
 RPMI 1640 Wang Y., Liu H., Guo H., Wen H., and Liu J.: Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich, World J Gastroenterol 10, 699, 2004
Rat Rat, SD, male, 200-250 g Hepatocytes Collagenase: 0.05%
 DMEM Raman Priya, Donkin Shawn S, Spurlock Michael E: Regulation of hepatic glucose metabolism by leptin in pig and rat primary hepatocyte cultures,Am J Physiol Regul Integr Comp Physiol Vol. 286,, , R206-16, 2004
Rat Rat, SD, male, 230-250 g Hepatocytes Collagenase Type 1: 0.1%
 HBSS Kuddus, R., Nalesnik, M., Subbotin, V., Rao, A., and Gandhi, C.: Enhanced Sythesis and Reduced Metabolism of Endothelin-1 (ET-1) by Hepatocytes - An Important Mechanism of Increased Endogenous Levels of ET-1 in Liver Cirrhosis, J Hepatol 33, 725, 2000
Rat Rat, male, 3 week Hepatocytes Collagenase Type 4: 0.02%
 Williams medium E Low-Baselli A., Hufnagl K., Parzefall W., Schulte-Hermann R., and Grasl-Kraupp B.: Initiated Rat Hepatocytes in Primary Culture: A Novel Tool to Study Alterations in Growth Control During the First Stage of Carcinogenesis, Carcinogenesis 21, 79, 2000
Rat Rat, SD, male, 450-500 g Stellate Protease: 0.02%
 HBSS Gabriel, A., Kuddus, R., Rao, A., and Gandhi, C.: Down-Regulation of Endothelin Receptors by Transforming Growth Factor B1 in Hepatic Stellate Cells, J Hepatol 30, 440, 1999
Rat Rat, Wistar, male Hepatocytes Collagenase Type 2: 120 u/ml
 HBSS Wolz E, Liechti H, Notter B, Oesterhelt G, Kistler A: Characterization of metabolites of astaxanthin in primary cultures of rat hepatocytes, Drug Metab Dispos 27, 456-62, 1999
Rat Rat, Lewis, adult Hepatocytes Collagenase: 60 u/ml
 HEPES Matsuura T, Gad MZ, Harrison EH, and Ross AC: Lecithin:retinol acyltransferase and retinyl ester hydrolase activities are differentially regulated by retinoids and have distinct distributions between hepatocyte and nonparenchymal cell fractions of rat liver, J Nutr 127, 218, 1997
Rat Rat Hepatocytes Collagenase: 0.04% - 0.06%
 PBS : Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:14.1, 1995
Rat Rat Hepatocytes Collagenase: 0.05%
 DMEM : Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:16.1, 1995
Rat Rat, SD, male, 200 g Hepatocytes Collagenase: 0.05%
 Medium 199 Rana, B., Mischoulon, D., Xie, Y., Bucher, N., and Farmer, S.: Cell-Extracellular Matrix Interactions Can Regulate the Switch between Growth and Differentiation in Rat Hepatocytes: Reciprocal Expression of C/EBPa and Immediate-Early Growth Response Transcription Factors, Mol Cell Biol 14 (9), 5858, 1994
Rat Rat, Wistar, adult, male, 100-125 g Hepatocytes Collagenase Type 2: 0.05%
 Serum-free medium Liu, J., McKim, J., Liu, Y., and Klaassen, C.: Effects of Butyrate Homologes on Metallothionein Induction in Rat Primary Hepatocyte Cultures, In Vitro Cell Dev Biol 28, 320, 1992
Rat Rat, SD, male, 250-350 g Hepatocytes Collagenase Type 4: 200 u/ml
 RPMI 1640 Dixit, V., Arthur, M., and Gitnick, G.: Repeated Transplantation of Microencapsulated Hepatocytes for Sustained Correction of Hyperbilirubinemia in Gunn Rats, Cell Transplant 1, 275, 1992
Rat Rat, SD, 8 - 12 day Parenchymal hepatocytes Collagenase Type 4: 80 u/ml
 HBSS Davila, J., Reddy, C., Davis, P. and Acosta, D.: Toxicity Assessment of Papaverine Hydrochloride and Papaverine-Derived Metabolites in Primary Cultures of Rat Hepatocytes, In Vitro Cell Dev Biol 26, 515, 1990
Rat Rat, SD, male, 250-350 g Hepatocytes Collagenase Type 2: 0.05%
 HBSS, CMF Liu, J., Kershaw, W., and Klaassen, C.: Rat Primary Hepatocyte Cultures are a Good Model for Examining Metallothionein-Induced Tolerance to Cadmium Toxicity, In Vitro Cell Dev Biol 26, 75, 1990
Rat Rat, Wistar, male, 200 g Parenchymal Collagenase Type 1:
HEPES, modified Kindberg, G., Gudmundsen, O., and Berg, T.: The Effect of Vandate on Receptor-mediated Endocytosis of Asialoorosomucoid in Rat Liver Parenchymal Cells, J Biol Chem 265, 8999, 1990
Rat Rat, SD, male, 175-225 g Hepatocytes Collagenase:
Kreb's Ringer bicarbonate buffer Reddy, S., Amick, G., Cooper, R., and Damun, Z.: Insulin Stimulates the Activity of a Protamine in Isolated Rat Hepatocytes, J Biol Chem 265, 7748, 1990
Rat Rat, SD, neonatal, 8 - 10 days Hepatocytes Collagenase Type 4: 0.05%
 Hanks' BSS, CF Davila, J., Lenherr, A., and Acosta, D.: Protective Effect of Flavonoids on Drug-Induced Hepatotoxicity In Vitro, Toxicology 57, 267, 1989
Rat Rat, Fischer 344, male, 14 months Hepatocytes Collagenase: 0.05%
 Ringer's biocarbonate buffer Nagy, I., Ohno-Iwashita, Y., Ohta, M., Nagy, V., Kitani, K., Ando, S., and Imahori, K.: Effect of Perfringolysin O on the Lateral Diffusion Constant of Membrane Proteins of Hepatocytes as Revealed by Fluorescence Recovery After Photobleaching, Biochim Biophys Acta 939, 551, 1988
Rat Rat, SD, male, 200-300 g Hepatocytes Collagenase: 0.05%
 HEPES Voss, A. and Sprecher, H.: Metabolism of 6,9,12-Octadecatetraenoic and 6,9,12,15-Octadecatetraenoic Acid, Biochim Biophys Acta 958, 153, 1988
Rat Rat, Wistar, male,3 month, 200 g Parenchymal Endothelial Kupffer Collagenase Type 1: 0.05%
 Krebs Henseleit Kuiper, J., Zijlstra, F., Kamps, J, and vanBerkel, T.: Identification of Prostaglandin D2 as the Major Eiconsanoid from Liver Endothelial and Kupffer Cells, Biochim Biophys Acta 959, 143, 1988
Rat Rat, Wistar, male, 200 - 300 g Parenchymal Kupffer Collagenase Type 2: 0.05%
 HBSS with CaCl2 Cai, H., He, Z., and Ding, Y.: Effects of Monocyte Macrophages Stimulation on Hepatic Lipoprotein Receptors, Biochim Biophys Acta 958, 334, 1988
Rat Rat, Wistar, female, 17 days Hepatocytes Collagenase: 0.05%
 HBSS Cotariu, D., Barr-Nea, L., Papo, N., and Zaidman, J.: Induction of gamma-Glutamyl Transferase by Dexamethasone in Cultured Rat Hepatocytes, Enzyme 40, 212, 1988
Rat Rat, CD strain, albino, male, 140 -180 g Hepatocytes and Nonparenchymal Pronase: 0.1%
 HEPES buffer with calcium Braun, L., Mead, J., Panzica, M., Mikumo, R., Bell, G., and Fausto, N.: Transforming Growth Factor B mRNA Increases During Liver Regeneration:A Possible Paracrine Mechanism Of Growth Regulation, Proc Natl Acad Sci U S A 85, 1539, 1988
Rat Rat, 250-350 g
(also guinea-pig & rabbit)		 Hepatocytes Collagenase Type 2: 166 u/ml
 DMEM Chang, T., and Chang, C.: Hepatic Uptake of Asialoglycoprotein is Different Among Mammalian Species Due to Different Receptor Distribution, Biochim Biophys Acta 942, 57, 1988
Rat Rat, SD, male, 180-200 g Hepatocytes Collagenase: 0.05%
 MEM Gupta, C., Hattori, A., Betschart, J., Virji, M., and Shinozuka, H.: Modulation of Epidermal Growth Factor Receptors in Rat Hepatocytes by Two Liver-Promoting Regimens, a Choline-Deficient and a Phenobarbital Diet, Cancer Res 48, 1162, 1988
Rat Rat, Fisher 344, 150-200 g Bile ductular epithelial Collagenase Type 1: 220 u/ml
 Leibovitz L-15 Mathis, G., Walls, S., and Sirica, A.: Biochemical Characteristics of Hyperplastic Rat Bile Ductular Epithelial Cells Cultured "on Top" and "Inside" Different Extracellular Matrix Substitutes, Cancer Res 48, 6145, 1988
Rat Rat, Wistar, male, 260-310 g Hepatocytes Collagenase Type 1: 0.05%
 HEPES McAbee DD and Weigel PH: ATP-dependent inactivation and reactivation of constitutively recycling galactosyl receptors in isolated rat hepatocytes, Biochem 27, 2061, 1988
Rat Rat, SD, male, 350-400 g Hepatocytes Collagenase Type 2:
HBSS Rodriguez de Turco, E., and Spitzer, J.: Kinetics of Diacylglycerol Accumulation in Response to Vasopressin Stimulation in Hepatocytes of Continuously Endotoxaemic Rats, Biochem J 253, 73, 1988
Rat Rat, SD, male, 350 - 450 g Lipocytes Kupffer Sinusoidal endothelial Collagenase: 0.015%
 DMEM/Ham's F-12 Friedman, S. and Roll, F.: Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan, Anal Biochem 161, 207, 1987
Rat Rat, SD, male Hepatocytes Collagenase: 100 - 200 µg/g body weight
 Eagle's
Eagle's w/HEPES
HBSS		 Oka, J. and Weigel, P.: Monensin Inhibits Ligand Dissociation Only Transiently and Partially and distinguishes two galactosyl receptor pathways in isolated rat hepatocytes, J Cell Physiol 133, 243, 1987
Rat Rat, Fischer, male, 150-250 g Hepatocytes Collagenase Type 1: 125 - 250 u/ml
 MEM
(see reference)		 Francavilla, A., Ove, P., Polimeno, L., Sciascia, C., Coetzee, M., and Starzi, T.: Epidermal Growth Factor and Proliferation in Rat Hepatocytes in Primary Culture Isolation at Different Times after Partial Hepatectomy, Cancer Res 46, 1318, 1986
Rat Rat, SD, female, 100-180 g Hepatocytes Collagenase: 0.5%
 Krebs Ringer bicarbonate buffer Schwarz, K., Lanier, S., Carter, E., Homcy, C., and Graham, R.: Rapid Reciprocal Changes in Adrenergic Receptors in Intact Isolated Hepatocytes During Primary Cell Culture, Mol Pharmacol 27, 200, 1985
Rat Rat, Lewis, female, 200-250 g Hepatocytes, Kupffer, endothelial Collagenase: 0.05%
 Gey's BSS Holstege A, Leser HG, Pausch J, Gerok W: Uridine catabolism in Kupffer cells, endothelial cells, and hepatocytes, Eur J Biochem 149, 169-73, 1985
Rat Rat, SD, male, 270 - 320 g Hepatocytes Collagenase: 0.04%
 Bicarbonate buffer with calcium added Brass, E., Garrity, M., and Robertson, R.: Inhibition of Glucagon-Stimulated Hepatic Glycogenolysis by E-Series Prostaglandins, FEBS Lett 169, 293, 1984
Rat Rat, Wistar, adult, male, 150-200 g Hepatocytes Trypsin: 0.005%
 Williams medium E Okumura, T. and Saito, K.: Degradation of Prostaglandin E2 in a Primary Culture of Adult Rat Hepatocytes, J Biochem (Tokyo) 96, 429, 1984
Rat Rat, Fischer, Lewis and SD, male 10-18 months Epithelial Trypsin: 0.05%
 CMF HBSS Herring, A., Raychaudhuri, R., Kelley, S., and Iybe, P.: Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats, In Vitro 19, 576, 1983
Rat Rat, Wistar, male, 3 month old Endothelial Kupffer Parenchymal Collagenase: 0.05%
 HBSS Nagelkenke, J., Barto, K., and Berkel, T.: In Vivo and in Vitro Uptake and Degradation of Acetylated Low Density Lipoprotein by Rat Liver Endothelial, Kupffer, and Parenchymal Cells, J Biol Chem 258 (20), 12221, 1983
Rat Rat, SD, male/female, 250-300 g Hepatocytes Collagenase: 100 u/ml
 Krebs Henseleit bicarbonate buffer Studer, R. and Borle, A.: Differences between Male and Female Rats in the Regulation of Hepatic Glycogenolysis. The Relative Role of Calcium and cAMP in Phosphorylase Activation by Catecholamines, J Biol Chem 257, 7987, 1982
Rat Rat, Wistar, 12 day Epithelial Trypsin: 0.05%
 HBSS, CMF Malan-Shibley, L., and Iype, P.: Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels, Exp Cell Res 131, 363, 1981
Rat Rat, Zucker, obese Hepatocytes Collagenase Type 2: 0.30%
 Dulbecco-Vogt arginine free Eagle's Goldstein, A., Palmer, J., and Johnson, P.: Primary Cultures of Fetal Hepatocytes from the Genetically Obese Zucker Rat: Protein Synthesis, In Vitro 17, 651, 1981
Rat Rat, SD Hepatocytes Collagenase: 90 u/ml
 CF EGTA perfusate Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies, In Vitro 17, 935, 1981
Rat Rat, Wistar, female, fetus Hepatocytes Collagenase: 0.025%
 HEPES buffer Gugen-Guillouzo, C., Tichonicky, L., Szajnert, M., and Kruh, J.: Changes in Some Chromatin and Cytoplastic Enzymes of Perinatal Rat Hepatocytes, In Vitro 16, 1, 1980
Rat Rat, SD, male, 250-300 g Parenchymal Collagenase Type 2: 0.05%
 Kreb's Henseleit bicarbonate buffer Yamada, S., Otto, P., Kennedy, D., and Whayne, T.: The Effects of Dexamethasone on Metabolic Activity of Hepatocytes in Primary Monolayer Culture, In Vitro 16, 559, 1980
Rat Rat, Wistar, male, 180-210 g Hepatocytes Hyaluronidase: 0.02%
 Kreb's buffer De Gerlache, J., Lans, M., Taper, H., and Roberfroid, M.: Separate Isolation of Cells from Nodules and Surrounding Parenchyma of the Same Precancerous Rat Liver: Biochemical and Cytochemical Characterization, Toxicology 18, 225, 1980
Rat Rat, SD, 7-10 day Liver Collagenase: 0.05%
 HBSS modified (see reference) Acosta, D., Anuforo, D., and Smith, R: J Tiss Cul Meth 6, 35, 1980
Rat Rat, Wistar, male, 200-250 g Hepatocytes Hyaluronidase: 460 u/ml
 Saline Poli, G., Gravela, E., Albano, E., and Dianzani, M.: Studies on Fatty Liver with Isolated Hepatocytes: II., The Action of Carbon Tetrachloride on Lipid Peroxidation, Protein and Triglyceride Synthesis and Secretion, Exp Mol Pathol 30, 116, 1979
Rat Rat, SD, female, 100-150 g Hepatocytes Collagenase Type 1: 0.065%
 DMEM
(see reference)		 Davis, R., Engelhorn, S., Pangburn, S., Weinstein, D., and Steinberg, D.: Very Low Density Lipoprotein Synthesis and Secretion by Cultured Rat Hepatocytes, J Biol Chem 254 (6), 2010, 1979
Rat Rat
(also chicken)		 Hepatocytes Collagenase Type 3 & 4:
HEPES Weigel, P., Schnaar, R., Kuhlenschmidt, M., Schmell, E., Lee, R., Lee, Y., and Roseman, S.: Adhesion of Hepatocytes to Immobilized Sugars, J Biol Chem 254 (21), 10830, 1979
Rat Rat, SD, male, 200 g Hepatocytes Collagenase Type 1: 100 u/ml
 Buffers 1 & 2
(see reference)		 Rubin, K., Kjellen, L., and Oslashbrink, B.: Intercellular Adhesion between Juvenile Liver Cells.. A Method to Measure the Formation of Stable Lateral Contacts Between Cells Attached to a Collagen Gel, Exp Cell Res 109, 413, 1977
Rat Rat (WAG/RIJ), female, 24, 30, and 37 months Parenchymal Collagenase Type 1: 0.05% - 0.06%
 HEPES buffer VanBezodijen, C., Grell, T., and Knook, D.: Effect of Age on Protein Synthesis by Isolated Liver Parenchymal Cells, Mech Ageing Dev 6, 293, 1977
Rat Rat, Fisher, adult, male Hepatocytes Collagenase Type 1: 100 u/ml
 HBSS
(see reference)		 Williams, G., Bermudez, E., and Scaramuzzino, D.: Rat Hepatocytes Primary Cell Cultures III. Improved Dissociation and Attachment Techniques and the Enchancement of Survival by Culture Medium, In Vitro 13 (12), 809, 1977
Rat Rat , Wistar, male, 250-300 g Hepatocytes Collagenase: 0.05%
 Hank's solution, CF Gravela, E., Poli, G., Albano, E., and Dianzani, M.: Studies of Fatty Liver with Isolated Hepatocytes, Exp Mol Pathol 27, 339, 1977
Rat Rat, Fischer, male, adult, 170-265 g Hepatocytes Collagenase: 0.05%-0.10%
 Williams medium E Laishes, B., and Williams, G.: Conditions Affecting Primary Cell Cultures of Functional Adult Rat Hepatocytes. I.. The Effect of Insulin, In Vitro 12, 521, 1976
Rat Rat, SD, male, 200-250 g Hepatocytes Collagenase Type 1: 0.05%
 MEM Witters, L., Alberico, L., and Acruch, J.: Insulin Regulation of Glycogen Synthase in the Isolated Rat Hepatocyte, Biochem Biophys Res Commun 69 (4), 997, 1976
Rat Rat, Fischer, adult, 200-250 g Epithelial Hyaluronidase: 0.0075%
 KCl-NaCl HEPES Buffer Williams, G., and Gunn, J.: Long-Term Culture of Adult Rat Liver Epithelial Cells, Exp Cell Res 89, 139, 1974
Rat Rat, neonate, 3 day Hepatocytes Trypsin: 0.25%
 PBS, CMF Bausher, J., and Schaeffer, W.: A Diploid Rat Liver Cell Culture. 1. Characterization and Sensitivity to Aflatoxin B1, In Vitro 9, 286, 1974
Rat Rat, SD, albino, male Parenchymal Hyaluronidase: 0.1%
 Hank's solution, CMF Bonney, R., Becker, J., Walker, P., and Potter, V.: Primary Monolayer Cultures of Adult Rat Liver Parenchymal Cells Suitable for Study of the Regulation of Enzyme Synthesis, In Vitro 9, 399, 1974
Rat Rat, embryos, 1-3 days Hepatocytes Hyaluronidase: 0.10%
 HBSS Gerschenson, L., Berliner, J., and Davidson, M.: The Isolation and Culture of Liver Cells, Methods Enzymol 32, 733, 1974
Rat Rat, SD, adult, male, 200-250 g Parenchymal Hyaluronidase: 0.10%
 HBSS, CF Howard, R., Lee, J., and Pesch, L.: The fine structure, potassium content, and respiratory activity of isolated rat liver parenchymal cells prepared by improved enzymatic techniques, J Cell Biol 57, 642, 1973
Rat Rat, SD, adult, male, 180-300 g Parenchymal Collagenase Type 1: 0.05%
 Hank's solution, CF Bissell, D., Hammaker, L., and Meyer, U.: Parenchymal Cells from Adult Rat Liver in Nonproliferating Monolayer Culture. I. Functional Studies, J Cell Biol 59, 722, 1973
Rat Rat, Wistar, 200-250 g Hepatocytes Hyaluronidase: 0.10%
 HBSS, CF Berg, T., Boman, D., and Seglen, P.O.: Induction of Tryptophan Oxygenase in Primary Rat Liver Cell Suspensions by Glucocorticoid Hormone, Exp Cell Res 72, 571, 1972
Rat Rat, SD, female, 130-160 g Hepatocytes Hyaluronidase: 0.08%
 HBSS, CF Johnson, M., Das, N., Butcher, F., and Fain, J.: The Regulation of Gluconeogenesis in Isolated Rat Liver Cells by Glucagon, Insulin, Dibutyryl Cyclic Adenosine Monophosphate, and Fatty Acids, J Biol Chem 247, 3229, 1972
Rat Rat, Fisher 344, pregnant (19-21 days gestation),
200-250 g		 Hepatocytes Collagenase: 0.3%
 Modified Eagle's w/ Serum Leffert, H., and Paul, D.: Studies on Primary Cultures of Differentiated Fetal Liver Cells, J Cell Biol 52, 559, 1972
Rat Rat, Wistar, male, 260-310 g Hepatocytes Collagenase: 0.01 - 0.08%
 HEPES Seglen, P.: Preparation of Rat Liver Cells, Exp Cell Res 74, 450, 1972
Rat Rat, Fisher, 10 day Epithelial-like Trypsin: 0.25%
 PBS Williams, G., Weisburger, E., and Weisburger, J.: Isolation and Long-Term Cell Culture of Epithelial-Like Cells from Rat Liver, Exp Cell Res 69, 106, 1971
Rat Rat, Wistar, male, 6-8 weeks, 80-160 G Hepatocytes Hyaluronidase: 1.0%
 HBSS, CMF Iype, P.: Cultures from Adult Rat Liver Cells. 1.Establishment of Monolayer Cell-Cultures from Normal Lver, J Cell Physiol 78, 281, 1971
Rat Rat, 100-200 g Hepatocytes Hyaluronidase: 0.10%
 HBSS, CF Haung, Y., and Ebner, K.: Induction of Tyrosine Aminotransferase in Isolated Liver Cells, Biochim Biophys Acta 191, 161, 1969
Rat Rat, SD, adult, 200-300 g Parenchymal Hyaluronidase: 0.10%
Collagenase Type 1: 0.05%
 HBSS, CF Berry, M., and Friend, D.: High Yield Preparation of Isolated Rat Liver Parenchymal Cells, J Cell Biol 43, 506, 1969