Miscellaneous - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Equine Equine Tendon Cells Collagenase Type 1: 0.1%
DMEM Nemoto, M., Kizaki, K., Yamamoto, Y., Oonuma, T. and Hashizume, K.: Tenascin-C Expression in Equine Tendon-derived Cells During Proliferation and Migration., J Equine Sci 24, 17-24, 2013
Human Human, 3-7 year Dental pulp stem cells Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
DMEM Salmon, B., Bardet, C, Khaddam, M. Naji, J., Coyac, B., Baroukh, B., Letourneur, F., Lesieur, J., Decup, F., Le Denmat, D., Nicoletti, A., Poliard, A, Rowe, P., Huet, E., Vital, S. and Linglart, A.: MEPE-Derived ASARM Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia., PLoS ONE 8, e56749, 2013
Human Human Dental pulp stem Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
DMEM/F12 Bonnamain, V., Thinard, R., Sergent-Tanguy, S., Huet, P., Bienvenu, G., Naveilhan, P., Farges, J. and Alliot-Licht, B.: Human Dental Pulp Stem Cells Cultured in Serum-Free Supplemented Medium., Front Physiol 4, 357, 2013
Human Human Fungiform taste papillae Collagenase Type 1: 550 u/ml
Elastase: 10 u/ml
Soybean Trypsin Inhibitor: 0.09%
see reference Ozdener, H., Spielman, A. and Rawson, N.: Isolation and Culture of Human Fungiform Taste Papillae Cells, J Vis Exp 63, e3730, 2012
Human Human Adipose derived stromal Collagenase Type 2: 0.075%
DMEM Panetta, N., Gupta, D., Kwan, M., Wan, D., Commons, G. and Longaker, M.: Tissue Harvest by Means of Suction-Assisted or Third-Generation Ultrasound-Assisted Lipoaspiration Has No Effect on Osteogenic Potential of Human Adipose-Derived Stromal Cells., Plast Reconstr Surg 124, 65, 2009
Human Human Oral epithelial Neutral Protease: 1.2 u/ml
Trypsin: 0.05%
PBS Nakamura, T., Endo, K. and Kinoshita, S.: Identification of Human Oral Keratinocyte Stem/Progenitor Cells by Neurotrophin Receptor p75 and the Role of Neurotrophin/p75 Signaling., Stem Cells 25, 628-38, 2007
Human Human Carotid artery plaque macrophage Collagenase Type 4: 450 u/ml
Deoxyribonuclease I: 500 u/ml
Soybean Trypsin Inhibitor: 0.1%
HBSS Patino WillmarD, Kang Ju-Gyeong, Matoba Satoaki, Mian OmarY, Gochuico BernadetteR, Hwang PaulM: Atherosclerotic plaque macrophage transcriptional regulators are expressed in blood and modulated by tristetraprolin, Circ Res 98, 1282-9, 2006
Human Human Esophageal microvascular endothelial Collagenase Type 2: 0.2%
MCDB-131 Rafiee, P., Ogawa, H., Heidemann, J., Li, M., Aslam, M., Lamirand, T., Fisher, P., Graewin, S., Dwinell, M., Johnson, C., Shaker, R. and Binion, D.: Isolation and Characterization of Human Esophageal Microvascular Endothelial Cells: Mechanisms of Inflammatory Activation., Am J Physiol Gastrointest Liver Physiol 285, G1277, 2003
Human Gastric epithelial cells Collagenase Type 2: 200 u/ml
Neutral Protease: 1.2 u/ml
Soybean Trypsin Inhibitor: 0.125%
L-15 Smoot, D., Sewchand, J., Young, K., Desbrodes, B., Allen, C. and Naab, T.: A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells, Meth Cell Sci 22, 133, 2000
Human Human Synoviocytes Collagenase: 0.2%
DMEM/F12 Chen V, Croft D, Purkis P, Kramer IM: Co-culture of synovial fibroblasts and differentiated U937 cells is sufficient for high interleukin-6 but not interleukin-1beta or tumour necrosis factor-alpha release., Br J Rheumatol 37, 148-56, 1998
Human Human Peptic cells Collagenase Type 4: 0.1%
Soybean Trypsin Inhibitor: 0.2%
Ringer solution Lanas. A., Nerín, J., Esteva, F. and Sáinz, R.: Non-Steroidal Anti-Inflammatory Drugs and Prostaglandin Effects on Pepsinogen Secretion by Dispersed Human Peptic Cells., Gut 36, 657-63, 1995
Human Human Periapical granuloma CLSPA: 0.25%
RPMI-1640 Stern MH, Dreizen S, Mackler BF, Levy BM: Isolation and characterization of inflammatory cells from the human periapical granuloma, J Dent Res 61, 1408-12, 1982
Insect Insect, Lepidoptera Lepitopteran Collagenase Type 3: 0.35%
Hyaluronidase: 0.01%
Dulbecco PBS Goodwin, R and McCawley, P: Initiating Attached Cell Lines From the Lepidoptera (Insecta), Meth Cell Sci 3, 567, 1977
Mouse Mouse, 26-30 g Myenteric plexus Collagenase Type 2: 0.13%
Neurobasal A Smith, T., Grider, J., Dewey, W. and Akbarali, H.: Morphine Decreases Enteric Neuron Excitability via Inhibition of Sodium Channels., PLoS ONE 7, e45251, 2012
Mouse Mouse, 10 week Synovial mesenchymal Collagenase: 0.1%
Deoxyribonuclease I: 0.005%
DMEM Futami, I., Ishijima, M., Kaneko, H., Tsuji, K., Ichikawa-Tomikawa, N., Sadatsuki, R., Muneta, T., Arikawa-Hirasawa, E., Sekiya, I. and Kaneko, K.: Isolation and Characterization of Multipotential Mesenchymal Cells from the Mouse Synovium., PLoS ONE 7, e45517, 2012
Mouse Mouse Spleen, bone marrow endothelial Collagenase Type 4: 0.3-1.0%
Deoxyribonuclease I: 20 u/ml
PBS Shi, C., Jia, T., Mendez-Ferrer, S., Hohl, T., Serbina, N., Lipuma, L., Leiner, I., Li, M., Frenette, P. and Pamer, E.: Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands., Immunity 34, 590, 2011
Mouse Mouse Dentritic Collagenase Type 2: 0.1%
Deoxyribonuclease I: 0.001%
DME Stock, A., Booth, S. and Cerundolo, V.: Prostaglandin E2 Suppresses the Differentiation of Retinoic Acid-Producing Dendritic Cells in Mice and Humans., J Exp Med 208, 761, 2011
Mouse Mouse Cochleaer Trypsin: 0.125%
DMEM/F-12 Jan, T., Chai, R., Sayyid, Z. and Cheng, A.: Isolating LacZ-Expressing Cells from Mouse Inner Ear Tissues Using Flow Cytometry., J Vis Exp 58, e3432, 2011
Mouse Mouse, 6-8 week Bone marrow mesenchymal stem Collagenase Type 1: 0.25%
RPMI 1640 Xu, S., De Becker, A., Van Camp, B., Vanderkerken, K. and Van Riet, I.: An Improved Harvest and In Vitro Expansion Protocol for Murine Bone Marrow-Derived Mesenchymal Stem Cells., J Biomed Biotechnol 2010, 105940, 2010
Mouse Mouse, 3-6 week Ear mesenchymal stem Collagenase Type 1: 0.2%
DMEM/F12 Staszkiewicz, J., Gimble, J., Manuel, J. and Gawronska-Kozak, B.: IFATS Collection: Stem Cell Antigen-1-Positive Ear Mesenchymal Stem Cells Display Enhanced Adipogenic Potential., Stem Cells 26, 2666, 2008
Mouse Mouse, 6-10 week Salivary Gland and Stomach Collagenase Type 4: 0.8%
Deoxyribonuclease I: 1.0%
RPMI-1640 Ji, H., Rintelen, F., Waltzinger, C., Meier, D, Bilancio, A., Pearce, W., Hirsch, E., Wymann, M., Ruckle, T., Camps, M., Vanhaesebroeck, B., Okkenhaug, K. and Rommel, C.: Inactivation of PI3Kgamma and PI3Kdelta Distorts T-cell Development and Causes Multiple Organ Inflammation., Blood 110, 2940, 2007
Mouse Mouse Bone marrow Collagenase Type 1: 0.15%
Neutral Protease: 0.15%
PBS Bertoncello, I. and Williams, B.: Hematopoietic Stem Cell Characterization by Hoechst 33342 and Rhodamine 123 Staining., Methods Mol Biol 263, 181, 2004
Mouse Mouse, 25-30 g Tracheal inflammatory cells Collagenase Type 4: 0.1%
Deoxyribonuclease I: 50 u/ml
Soybean Trypsin Inhibitor: 0.1%
RPMI 1640 Minamoto Kanji, Pinsky DavidJ: Recipient iNOS but not eNOS deficiency reduces luminal narrowing in tracheal allografts, J Exp Med 196, 1321-33, 2002
Porcine Porcine, 6 month Synoviocytes Hyaluronidase: 660 u/ml
Trypsin: 0.25%
Collagenase Type 2: 583 u/ml
DMEM Kean, T and Dennis, J.: Synoviocyte Derived-Extracellular Matrix Enhances Human Articular Chondrocyte Proliferation and Maintains Re-Differentiation Capacity at Both Low and Atmospheric Oxygen Tensions., PLoS ONE 10, e0129961, 2015
Rabbit Rabbit, New Zealand, 8-10 week Tenocytes and tendon stem cells Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
DMEM Zhang, J. and Wang, J.: Characterization of Differential Properties of Rabbit Tendon Stem Cells and Tenocytes., BMC Musculoskelet Disord 11, 10, 2010
Rat Rat, Lewis, M Synovial macrophages Collagenase Type 1: 250 u/ml
DMEM Jain, S., Tran, T. and Amiji, M.: Macrophage Repolarization with Targeted Alginate Nanoparticles Containing IL-10 Plasmid DNA for the Treatment of Experimental Arthritis., Biomaterials 61, 162-77, 2015
Rat Rat, Wistar, 7-9 week Gingival mitochondria Collagenase Type 1: 0.115-0.130 %
HBSS Kaneko, N., Rikimaru, T., Fujimura, T., Mori, S., Hidaka, S. and Kaya, H.: Preparation of Rat Gingival Mitochondria with an Improved Isolation Method., Int J Dent 2010, 275103, 2010
Rat Rat, male, 250-350g Submandibular acinar Collagenase: 0.05%
PBS Silver, N., Proctor, GB, Arno, M. and Carpenter GH: Activation of mTOR Coincides with Autophagy During Ligation-induced Atrophy in the Rat Submandibular Gland., Cell Death Dis 1, e14, 2010
Rat Rat, 8-10 week Synovial cells Collagenase Type 1: 250 u/ml
RPMI 1640 Moghaddami, M., Cleland, L. and Mayrhofer, G.: MHC II+ CD45+ Cells from Synovium-Rich Tissues of Normal Rats: Phenotype, Comparison with Macrophage and Dendritic Cell Lineages and Differentiation into Mature Dendritic Cells In Vitro., Int Immunol 17, 1103, 2005