Neural - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine
(also rat)		 Heart
Adrenal chromaffin
Paraneurons		 Trypsin: 0.06%
 25mM HEPES buffered Locke's solution, CMF Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990
Bovine Bovine Microvascular endothelial Collagenase/Dispase: 0.1%
 MEM Bowman, P., Betz, A., and Goldstein, G.: Primary Culture of Microvascular Endothelial Cells From Bovine Retina, In Vitro 18 (7), 626, 1982
Bovine Calf
(also lamb)		 Oligodendroglia
Neural		 Trypsin: 0.1%
 (see reference) Poduslo, S., Miller, K., and McKhann, G.: Metabolic Properties of Maintained Oligodendroglia Purified from Brain, J Biol Chem 253, 1592, 1978
Chicken Chicken, White Leghorn, embryos, 17-21 day Neurons Papain: 40 u/ml
 HEPES Raman, I., and Trussell, L.: The Kinetics of the Response to Glutamate and Kainte in Neurons of the Avian and Cochlear Nucleus, Neuron 9, 173, 1992
Chicken Chick, embryo, 10-14 day Flat, retina Trypsin: 0.1%
 Tyrode's solution, CMF Moyer, M., Bullrich, F., and Sheffield, J.: Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina, In Vitro Cell Dev Biol 26, 1073, 1990
Chicken Chick, White Leghorn, embryo
(also rat, SD, embryo)		 Spinal cord Trypsin: 0.05%
 Phosphate buffer
(see reference)		 Schnaar, R., and Schaffner, A.: Separation of cell types from embryonic chicken and rat spinal cord: characterization of motoneuron-enriched fractions, J Neurosci 1, 204, 1981
Chicken Chick Dorsal root ganglion neurons
Spinal cord		 Trypsin: 0.1%
 Puck's saline, CMF Choi, D., and Fiscbach, G.: GABA Conductance of Chick Spinal Cord and Dorsal Root Ganglion Neurons in Cell Culture, J Neurophysiol 45, 605, 1981
Chicken Chick, White Leghorn embryo, 8 day Neurons, ganglia Trypsin: 0.25%
 HBSS, CMF Bottenstein, J., Skaper, S., Varon, S., and Sato, G.: Selective Survival of Neurons from Chick Embryo Sensory Ganglionic Dissociates Utilizing Serum-Free Supplemented Medium, Exp Cell Res 125, 183, 1980
Chicken Chicken, White Leghorn, fertile eggs Ciliary ganglion neurons Trypsin: 0.25%
 Eagle's MEM Tuttle, J., Suszkiw, J., and Ard, M.: Long-Term Survival and Development of Dissociated Parasympathetic Neurons in Culture, Brain Res 183, 161, 1980
Chicken Chicken, embryos, 9-10 days old Dorsal root ganglia neurons Collagenase: 0.01%
 Eagle's MEM Mudge, A., Leeman, S., and Fischbach, G.: Enkephalin Inhibits Release of Substance P From Sensory Neurons in Culture and Decreases Action Potential Duration, Proc Natl Acad Sci U S A 76 (1), 526, 1979
Chicken Chick embryos Ganglion chains, sympathetic ganglia Trypsin: 0.25%
 Krebs Phosphosaline McCarthy, K., and Partlow, L.: Preparation of Pure Neuronal and Non-Neuronal Cultures From Embryonic Chick Sympathetic Ganglia: A New Method Based on Both Differential Cell Adhesiveness and the Formation of Homotypic Neuronal Aggregates, Brain Res 114, 391, 1976
Fish Black ghose knige fish, adult (Apteronotus albifrons) Neurons, spinal cord Trypsin: 0.4%
 PBS, CMF Anderson, M.J.: Differences in Growth of Neurons from Normal and Regenerated Teleost Spinal Cord in vitro, In Vitro Cell Dev Biol 29A, 145, 1993
Frog Xenopus, embryonic Neuron Collagenase Type 1: 0.1%
 Steinberg's solution Takahashi, T., Nakajima, Y., Hirosawa, K., Nakajima, S., and Onodera, K.: Structure and Physiology of Developing Neuromuscular Synapses in Culture, J Neurosci 7, 473, 1987
Guinea-Pig Guinea-pig, newborn Neuron, enteric Trypsin: 0.125%
 Medium 199 Jessen, K, McConnell, J., Purves, R., Burnstock, G., and Chamley-Campbell, J.: Tissue Culture of Mammalian Enteric Neurons, Brain Res 152, 573, 1978
Hamster Hamster, male Vomeronasal organ neurons Collagenase Type 1: 0.02%
Trypsin: 0.02%
 PBS Liman ER: Regulation by voltage and adenine nucleotides of a Ca2+-activated cation channel from hamster vomeronasal sensory neurons, J Physiol 548, 777, 2003
Human Human Neural, various Papain: 12 u/ml
Trypsin: see reference
Collagenase/Dispase: see reference
 see reference Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S: Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24, Stem Cells 25, 1560-70, 2007
Human Human, 9 month Neurons Collagenase Type 4: 1.33%
Papain: 0.07 u/ml
Neutral Protease: 1 mg/ml
 DMEM/F12 Dietrich J, Lacagnina M, Gass D, Richfield E, Mayer-Proschel M, Noble M, Torres C, Proschel C.: EIF2B5 mutations compromise GFAP+ astrocyte generation in vanishing white matter leukodystrophy, Nat Med 11, 277, 2005
Human Human, 26 week Neural progenitor cells Papain: 2.5 u/ml
Deoxyribonuclease I: 250 u/ml
Neutral Protease: 1 u/ml
 DMEM/F-12 Fuja TJ, Schwartz PH, Darcy D, Bryant PJ.: Asymmetric localization of LGN but not AGS3, two homologs of Drosophila pins, in dividing human neural progenitor cells, J Neurosci Res 75, 782, 2004
Human Human, adult Ventricular epithelial Papain: 11.4 u/ml
Deoxyribonuclease I: 10 u/ml
 DMEM/F12 Roy NS, Benraiss A, Wang S, Fraser RA, Goodman R, Couldwell WT, Nedergaard M, Kawaguchi A, Okano H, Goldman SA: Promoter-targeted selection and isolation of neural progenitor cells from the adult human ventricular zone, J Neurosci Res 59, 321, 2000
Human Human, 5-65 years Retinal pigment epithelial (RPE) Trypsin: 0.25%
 HBSS Von Recum, H., Okano, T., Kim, S, and Bernstein, P.: Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture, Exp Eye Res 69, 97, 1999
Human Human Dorsal root ganglion neurons Collagenase Type 1: 0.2%
Neutral Protease: 0.5%
 DMEM/F-12 Dib-Hajj SD, Tyrrell L, Cummins TR, Black JA, Wood PM, Waxman SG.: Two tetrodotoxin-resistant sodium channels in human dorsal root ganglion neurons, FEBS Lett 462, 117, 1999
Mouse Mouse, embryonic Dopaminergic neurons Trypsin: 0.1%
Deoxyribonuclease I: 0.02%
 DMEM Radad Khaled, Gille Gabriele, Rausch Wolf-Dieter: Dopaminergic neurons are preferentially sensitive to long-term rotenone toxicity in primary cell culture, Toxicol In Vitro 22, 68-74, 2008
Mouse Mouse, 6 mo Neurons, neurospheres Papain: 0.2%
 Hibernate Brewer Gregory J, Torricelli John R: Isolation and culture of adult neurons and neurospheres, Nat Protoc 2, 1490-8, 2007
Mouse Mouse Neural, various Papain: 12 u/ml
Trypsin: see reference
Collagenase/Dispase: see reference
 see reference Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S: Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24, Stem Cells 25, 1560-70, 2007
Mouse Mouse, C57BL/6, 1 year Neurons Papain: 0.2%
 DMEM Eide, L, and McMurray, C: Culture of Adult Mouse Neurons, Biotechniques 38(1), 99-104, 2005
Mouse Mouse, adult Brain and spinal cord cells Trypsin: 0.25%
 PBS Gonzalez John M, Bergmann Cornelia C, Fuss Babette, Hinton David R, Kangas Cindy, Macklin Wendy B, Stohlman Stephen A: Expression of a dominant negative IFN-gammareceptor on mouse oligodendrocytes, Glia 51, 22-34, 2005
Mouse Mouse, neonatal Neurons PDS kit:
EBSS Gill JC, Moenter SM, Tsai PS: Developmental regulation of gonadotropin-releasing hormone neurons by fibroblast growth factor signaling, Endocrinology 145, 3830, 2004
Mouse Mouse, C57BL Cerebellar granule cell precursors Papain: 16.5 u/ml
Deoxyribonuclease I: 0.008%
 Dulbecco's PBS Okano-Uchida T, Himi T, Komiya Y, and Ishizaki Y: Cerebellar granule cell precursors can differentiate into astroglial cells, Proc Natl Acad Sci U S A 101, 1211, 2004
Mouse Mouse, 1-3 day Neurons, ganglia Papain: 20 u/ml
Deoxyribonuclease I: 100 u/ml
Collagenase: 0.3%
Trypsin: 0.05%
 HBSS Savchenko V, Sung U, Blakely RD.: Cell surface trafficking of the antidepressant-sensitive norepinephrine transporter revealed with an ectodomain antibody, Mol Cell Neurosci 24, 1131, 2003
Mouse Mouse, postnatal-day-1-old (P1) Neurons Trypsin: 0.25%
 NGF-containing medium Deshmukh, M., Kuida, K., and Johnson Jr., E.: Caspase Inhibition Extends the Commitment to Neuronal Death Beyond Cytochrome c Release to the Point of Mitochondrial Depolarization, J Cell Biol 150 (1), 131, 2000
Mouse Mouse embryos Neurons, DRG and SCG Trypsin: 0.25%
 L-15 medium Lee, K., Davies, A., and Jaenisch, R.: P75-Eficient Embryonic Dorsal Root Sensory and Neonatal Sympathetic Neurons Display a Decreased Sensitivity to NGF, Development 120, 1027, 1994
Mouse mouse, 65 days Neurons, neuronal precursors Trypsin: 0.1%
Deoxyribonuclease I: 0.001%
 DMEM Richards L., Kilpatrick T., and Bartlett P.: De Novo Generation of Neuronal Cells from the Adult Mouse Brain, Proc Natl Acad Sci U S A 89, 8591, 1992
Mouse Mouse, CD-1, neonate Neurons, dorsal root ganglion Trypsin: 0.25%
 HBSS Quinn, S. and De Boni, U.: Enhanced Neuronal Regeneration by Retinoic Acid of Murine Dorsal Root Ganglia and of Fetal Murine and human Spinal Cord in vitro, In Vitro Cell Dev Biol 27, 55, 1991
Mouse Mouse, fetal Precursor Trypsin: 0.5%
 PBS Kitani, H., Shiurba, R., Sakakura, T., Tomooka, Y.: Isolation and Characterization Of Mouse Neural Precursor Cells in Primary Culture, In Vitro Cell Dev Biol 27, 615, 1991
Mouse Mouse (SWR or CF1), 1-3 months Papillae, taste receptor Pronase E: 0.15%
 Carbonate-Phosphate buffer
(see reference)		 Spielman, A., Mody, I., Brand, J., Whitney, G., MacDonald, J., and Salter, M.: A Method for Isolating and Patch-Clamping Single Mammalian Taste Receptor Cells, Brain Res 503, 326, 1989
Mouse Mouse, neonatal
(also chick)		 PNS test neurons Trypsin: 0.08%
 Eagle's Basal Medium (see reference) Varon, S., Skaper, S., Barbin, G., Selak, I., and Manthorpe, M.: Low Molecular Weight Agents Support Survival of Cultured Neurons From the Central Nervous System, J Neurosci 4 (3), 654, 1984
Mouse Mouse (BALB/c), adult Neurons, spinal cord Collagenase Type 3: 0.25%
 Hank's BSS, CMF Eagleson, K. and Bennett, M.: Survival of Purified Motor Neurons In Vitro: Effects of Skeletal Muscle-Conditioned Medium, Neurosci Lett 38, 187, 1983
Mouse Mouse, 0-30 day Neural Trypsin NF 1:250: 50 0.25%
 BSS Shrier, B., Wilson, S., and Nirenberg, M.: Cultured Cell Systems and Methods for Neurobiology, Vol. 32,, , 765, 1974
Porcine Porcine, adult, 60-100 kg Superior cervical ganglia Papain: 2 u/ml
Collagenase: 0.12%
Neutral Protease: 0.48%
 HBSS Si ML, Lee TJ.: Presynaptic alpha7-nicotinic acetylcholine receptors mediate nicotine-induced nitric oxidergic neurogenic vasodilation in porcine basilar arteries, J Pharmacol Exp Ther 298, 122, 2001
Quail Quail Neural crest Trypsin: 0.05%
 MEM, HBSS Sieber-Blum, M., and Cohen, A.: Clonal Analysis of Quail Neural Crest Cells: They are Pluripotent and Differentiate in Vitro in the Absence of Noncrest Cells, Dev Biol 80, 96, 1980
Rat Rat, embryonic Hypothalamic neurons and glias Papain: 0.5 u/ml
 DMEM Yokosuka Makoto, Ohtani-Kaneko Ritsuko, Yamashita Kayoko, Muraoka Daisuke, Kuroda Yoichiro, Watanabe Chiho: Estrogen and environmental estrogenic chemicals exert developmental effects on rat hypothalamic neurons and glias, Toxicol In Vitro 22, 1-9, 2008
Rat Rat, Wistar, 4 day Hippocampal neurons PDS kit:
Neurobasal A Obradovic Darja, Gronemeyer Hinrich, Lutz Beat, Rein Theo: Cross-talk of vitamin D and glucocorticoids in hippocampal cells, J Neurochem 96, 500-9, 2006
Rat Rat, 2 month Spinal cord progenitor cells
Hybrid toxins		 PDS kit: see reference
 Neurobasal A Mothe, A., Kulbatski, I., Van Bendegem, R., Lee, L., Kobayashi, E., Keating, A., and Tator, C.: Analysis of Green Fluorescent Protein Expression in Transgenic Rats for Tracking Transplanted Neural Stem/Progenitor Cells, J Histochem Cytochem 53(10), 1215, 2005
Rat Rat, SD, E19 Dorsal root ganglia PDS kit:
MEM/Ham's F12 Gavva NR, Tamir R, Qu Y, Klionsky L, Zhang TJ, Immke D, Wang J, Zhu D, Vanderah TW, Porreca F, Doherty EM, Norman MH, Wild KD, Bannon AW, Louis JC, Treanor JJ.: AMG 9810 [(E)-3-(4-t-butylphenyl)-N-(2,3-dihydrobenzo[b][1,4] dioxin-6-yl)acrylamide], a novel vanilloid receptor 1 (TRPV1) antagonist with antihyperalgesic properties, J Pharmacol Exp Ther 313, 474, 2005
Rat Rat, Wistar, E15 Vomeronasal receptor neurons Collagenase/Dispase: 0.1%
Papain: 0.5 u/ml
 DMEM/F12 Moriya-Ito K, Osada T, Ishimatsu Y, Muramoto K, Kobayashi T, Ichikawa M: Maturation of vomeronasal receptor neurons in vitro by coculture with accessory olfactory bulb neurons, Chem Senses 30, 111, 2005
Rat Rat, SD, 1-2 day Cortical astrocytes Papain: see reference
 DMEM Floyd Candace L, Gorin Fredric A, Lyeth Bruce G: Mechanical strain injury increases intracellular sodium and reverses Na+/Ca2+ exchange in cortical astrocytes, Glia 51, 35-46, 2005
Rat Rat, SD, 0-2 day Superior cervical ganglion Collagenase Type 4: 20 u/ml
Trypsin: 0.25%
 DMEM Pedraza Carlos E, Podlesniy Petar, Vidal Noemi­, Arevalo Juan Carlos, Lee Ramee, Hempstead Barbara, Ferrer Isidre, Iglesias Montse, Espinet Carme: Pro-NGF isolated from the human brain affected by Alzheimer's disease induces neuronal apoptosis mediated by p75NTR, Am J Pathol 166, 533-43, 2005
Rat Rat, fetal Brainstem and cortical neurons PDS kit:
DMEM Lovshin JA, Huang Q, Seaberg R, Brubaker PL, Drucker DJ: Extrahypothalamic expression of the glucagon-like peptide-2 receptor is coupled to reduction of glutamate-induced cell death in cultured hippocampal cells, Endocrinology 145, 3495, 2004
Rat Rat, SD, male 150-220 g Dorsal root ganglion neurons Collagenase Type 4: 0.125%
Trypsin: 0.05%
 DMEM/Ham's F12 Hu Hong-Zhen, Gu Qihai, Wang Chunbo, Colton Craig K, Tang Jisen, Kinoshita-Kawada Mariko, Lee Lu-Yuan, Wood Jackie D, Zhu Michael X: 2-aminoethoxydiphenyl borate is a common activator of TRPV1, TRPV2, and TRPV3, J Biol Chem 279, 35741-8, 2004
Rat Rat, 15 week Neurons Papain: 0.2%
 Hibernate A Evans J, Sumners C, Moore J, Huentelman MJ, Deng J, Gelband CH, and Shaw G: Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem, J Neurophysiol 87, 1076, 2002
Rat Rat, SD, male, 270-330 g Spinal progenitor cells PDS kit: with modifications
 Neurobasal medium Lin CR, Wu PC, Shih HC, Cheng JT, Lu CY, Chou AK, Yang LC.: Intrathecal spinal progenitor cell transplantation for the treatment of neuropathic pain, Cell Transplant 11, 17, 2002
Rat Rat, Fisher, 8-9 week Adult progenitor Papain: 2.5 u/ml
Deoxyribonuclease I: 250 u/ml
Neutral Protease: 1 u/ml
 DMEM/F-12 Lie DC, Dziewczapolski G, Willhoite AR, Kaspar BK, Shults CW, Gage FH.: The adult substantia nigra contains progenitor cells with neurogenic potential, J Neurosci 22, 6639, 2002
Rat Rat Neurons, hippocampal Papain: 15 - 20 u/ml
 Eagle's MEM
(see reference)		 Liu, Q., Kawai, H., and Berg, D.: B-Amyloid Peptide Blocks the Response of a7-Containing Nicotinic Receptors on Hippocampal Neurons, PNAS 98 (8), 4734, 2001
Rat Rat, newborn, 7 days old Neurons Trypsin: 0.25%
 MEM10 Acosta, C., Fabrega, A., Masco, D., and Lopez, H.: A Sensory Neuron Subpopulation with Unique Sequential Survival Dependence on Nerve Growth Factor and Basic Fibroblast Growth Factor during Development, J Neurosci 21 (22), 8873, 2001
Rat Rat, embryonic 18-19 day Hippocampal neurons PDS kit: see reference
 DMEM Mabuchi T, Kitagawa K, Kuwabara K, Takasawa K, Ohtsuki T, Xia Z, Storm D, Yanagihara T, Hori M, Matsumoto M: Phosphorylation of cAMP response element-binding protein in hippocampal neurons as a protective response after exposure to glutamate in vitro and ischemia in vivo, J Neurosci 21, 9204-13, 2001
Rat Rat, embryonic, day 18 Cortical Papain:
Neurobasal medium and DMEM O'Connor, S., Andreadis, J., Shaffer, K., Ma, W., Pancrazio, J., and Stenger, D.: Immobilization of Neural Cells in Three-Dimensional Matrices for Biosensor Applications, Biosensors & Bioelectronics 14, 871, 2000
Rat Rat, Wister, postnatal, P1-3 days Neurons, hippocampal Papain: 20 u/ml
 EBSS Neuhoff, H., Roeper, J., Schweizer, M.: Activity-Dependent Formation of Perforated Synapses in Cultured Hippocampal Neurons, Eur J Neurosci 11, 4241, 1999
Rat Rat, SD, female, pregnant Sciatic nerves Trypsin: 0.025%
 L-15 medium
(see reference)		 Morrison, S., White, P., Zock, C., and Anderson, D.: Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells, Cell 96, 737, 1999
Rat Rat
(also mice)		 Spinal cord Trypsin: 0.133%
 HBSS and PIPES Johansson, C., Momma, S., Clarke, D., Risling, M., Lendahl, U., and Frisen, J: Identification of a Neural Stem Cell in the Adult Mammalian Central Nervous System, Cell 96, 25, 1999
Rat Rat, (Long Evans), 2-5 day old Neurons, hippocampal Papain: 20 u/ml
 EBSS Wilding, T., and Huettner, J.: Activation and Desensitization of Hippocampal Kainate Receptors, J Neurosci 17 (8), 2713, 1997
Rat Rat, Wister and SD, newborn, 0-21 days, either sex Myenteric ganglia Trypsin: 0.05%
 MEM-HEPES Schafer, K., Saffrey, M., Burnstock, G., and Mastres-Ventura, P.: A New Method for the Isolation of Myenteric Plexus from the Newborn Rat Gastrointestinal Tract, Brain Res Proto 1, 109, 1997
Rat Rat, pups, 24-48 h old Neurons, hippocampal Papain: 20 u/ml
 Harvest buffer Hall, R., and Soderling, T.: Differential Surface Expression and Phosphorylation of the N-Methyl-D-Aspartate Receptor NR1 and NR2 in Cultured Hippocampal Neurons, J Biol Chem 272 (7), 4135, 1997
Rat Rat, SD, adult, 250-300g, P8 Neurons, DRG Neutral Protease: 0.5%
 L-15 w/ CO2 Davies, S., Fitch, M., Memberg, S., Hall, A, Raisman, G., and Silver, J.: Regeneration of Adult Axons in White Matter Tracts of the Central Nervous System, Nature 390, 680, 1997
Rat Rat, 1-4 day old Neurons, hippocampal Papain: 20 u/ml
 MEM Twitchell, W., Brown, S., and Mackie, K.: Cannabinoids Inhibit N- and P/Q-Type Calcium Channels in Cultured Rat Hippocampal Neurons, J Neurophysiol 78, 43, 1997
Rat Rat, SD, Fisher Hippocampal neurons Papain: 0.2%
 HibernateA/B27 Brewer, G.J.: Isolation and culture of adult rat hippocampal neurons of any age, J Neurosci Methods 71, 143, 1997
Rat Rat, E18 Hippocampal neurons Papain: 20 u/ml
Deoxyribonuclease I: 0.01%
 MEM Liu QY, Schaffner AE, Li YX, Dunlap V, Barker JL: Upregulation of GABAA current by astrocytes in cultured embryonic rat hippocampal neurons, J Neurosci 16, 2912-23, 1996
Rat Rat, pups, 14 day old Neurons, sympathetic Neutral Protease: 0.24%
 HBSS McFarlane, S., and Cooper, E.: Extrinsic Factors Influence the Expression of Voltage-Gated K Currents on Neonatal Rat Sympathetic Neurons, J Neurosci 13 (6), 2591, 1993
Rat Rats, SD, 11-14 day old, 29-32 g Basal forebrain neurons Trypsin: 0.125%
 Gey's BSS Allen, T., Sim, J., and Brown, D.: The Whole-Cell Calcium Current in Acutely Dissociated Magnocellular Cholinergic Basal Forebrain Neurones of the Rat, J Physiol 460, 91, 1993
Rat Rat, Wistar-Hanover, 7-12 day Postnatal dopamine neurons Trypsin: 0.035%
 (see reference) Rayport, S., Sulzer, D., Shi, W., Sawasdikosol, S., Monaco, J., Batson, D., and Rajendran, G.: Identified Postnatal Mesolimbic Dopamine Neurons in Culture: Morphology and Electrophysiology, J Neurosci 12 (11), 4264, 1992
Rat Rat, SD, 18-day-old, fetus Hippocampal Trypsin: 0.2%
 HBSS Cheng, B., and Mattson, M.: IGF-I and IGF-II Protect Cultured Hippocampal and Septal Neurons Against Calcium-Mediated Hypoglycemic Damage, J Neurosci 12 (4), 1558, 1992
Rat Rat Stem, neural crest Collagenase: 0.075%
 Ringer's solution Stemple, D., and Anderson, D.: Isolation of a Stem Cell for Neurons and Glia from the Mammalian Neural Crest, Cell 71, 973, 1992
Rat Rat, SD, pregnant, Charles River Pyramidal neurons
Nonpyramidal neurons		 Trypsin: 0.027%
 HEPES Buchhalter, J., and Dichter, M.: Electrophysiological Comparison of Pyramidal and Stellate Nonpyramidal Neurons in Dissociated Cell Culture of Rat Hippocampus, Brain Res Bull 26, 333, 1991
Rat Rat, SD, embryos, 19-21 days gestation Schwann, dorsal root ganglia Trypsin: 0.25%
 HBSS, CMF Mithen, F., Reiker, M., and Birchem, R.: Effects of Ethanol on Rat Schwann Cell Proliferation and Myelination in Culture, In Vitro Cell Dev Biol 26, 129, 1990
Rat Rat
(also bovine)		 Heart
Adrenal chromaffin
Paraneurons		 Trypsin: 0.06%
 25mM HEPES buffered Locke's solution, CMF Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990
Rat Rat, SD, 8 day old pups Cerebellar neurons Trypsin: 0.025%
 Eagle's MEM Novelli, A., Reilly, J., Lysko, P., and Henneberry, R.: Glutamate Becomes Neurotoxic Via the N-methyl-D-aspartate Receptor When Intracellular Energy Levels are Reduced, Brain Res 451, 205, 1988
Rat Rat, fetuses, 18 day old Hippocampal neurons Trypsin: 0.2%
 Eagle's MEM Mattson, M., and Kater, S.: Isolated Hippocampal Neurons in Cryopreserved Long-Term Cultures: Development of Neuroarchitecture and Sensitivity to Nmda, Int J Dev Neurosci 6 (5), 439, 1988
Rat Rat, postnatal Septal neurons Papain: 0.05%
 PBS, CMF Hatanaka,H., Tsukui, H., Nihonmatsu, I.: Septal Cholinergic Neurons From Postnatal Rat Can Survive In The Dissociate Culture Conditions In The Presence Of Nerve Growth Factor, Neurosci Lett 79, 85, 1987
Rat Rat, Long Evans, 1-15 days Neurons, visual cortex Papain: 20 u/ml
 BSS see reference (see reference) Huettner, J., and Baughman, R.: Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats, J Neurosci 6, 3044, 1986
Rat Rat, SD, female, 4 month old CNS cells Trypsin: 0.25%
 EBSS Wood, P., and Bunge, R.: Evidence That Axons are Mitogenic for Oligodendrocytes Isolated From Adult Animals, Nature 320, 756, 1986
Rat Rat, embryo, 15 day Dorsal horn neurons
Spinal		 Trypsin: 0.025%
 Ham's F-12 Jahr, C. and Jessell, T.: Synaptic Transmission between Dorsal Root Ganglion and Dorsal Horn Neurons in Culture: Antagonism of Monosynaptic Excitatory Postsynaptic Potentials and Glutamate Excitation by Kynurenate, J Neurosci 5, 2281, 1985
Rat Rat, fetus, 18-20 day Hippocampal neurons Trypsin: 0.25%
 HBSS, CMF Bartlett, W. and Banker, G.: An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts, J Neurosci 4, 1944, 1984
Rat Rat, postnatal Ganglion, retina Papain: 12.5 u/ml
 HBSS w/5 mM HEPES Leifer, D., Lipton, S., Barnstable, C., and Masland, R.: Monoclonal Antibody to Thy-1 Enhances Regeneration of Processes by Rat Retinal Ganglion Cells in Culture, Science 224, 303, 1984
Rat Rat, SD, neonatal Retinal ganglion Trypsin: 0.25%
 CMF medium /Ham's F-10 with FCS Sarthy, P.V., Curtis, B.M., and Catterall, W.A.: Retrograde Labeling, Enrichment, and Characterization of Retinal Ganglion Cells from the Neonatal Rat, J Neurosci 3, 2532, 1983
Rat Rat, SD, pups Retina Trypsin: 0.25%
 Ham's F-12 Sarthy PV, Curtis BM, and Catterall WA.: Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat, J Neurosci 3 (12), 2532, 1983
Rat Rat, W/FU, 5-8 day Neurons and glial Trypsin: 0.25%
 MEM
(see reference)		 Raff, M., Fields, K., Hakomori, S., Mirsky, R., Pruss, R., and Winter, J.: Cell-Type-Specific Markers for Distinguishing and Studying Neurons And the Major Classes of Glial Cells in Culture, Brain Res 174, 283, 1979
Rat Rat, fetus Neurons, sympathetic Trypsin: 0.25%
 L-15 or HBSS, CMF Wakshull, E., Johnson, M., Burton, H.: Postnatal Rat Sympathetic Neurons In Culture. 1. A Comparison With Embryonic Neurons, J Neurophysiol 42, 1410, 1979
Rat Rat, Wistar/Furth, newborn
(also bovine)		 Schwann Trypsin: 0.25%
 DMEM Brockes, J., Fields, K., and Raff, M.: Studies on Cultured Rat Schwann Cells. I. Establishment of Purified Populations From Cultures of Peripheral Nerve, Brain Res 165, 105, 1979
Rat Rat, embryo Neurons, cortical Trypsin: 0.027%
 MEM Dichter, M.: Rat Cortical Neurons in Cell Culture: Culture Methods, Cell Morphology, Electrophysiology, and Synapse Formation, Brain Res 149, 279, 1978
Rat Rat, newborn Neurons, sympathetic Collagenase Type 1: 0.01%
 Hank's solution, CF Reichardt, L., Patterson, P.: Neurotransmitter Synthesis and Uptake by Isolated Sympathetic Neurones in Microcultures, Nature 270, 147, 1977
Rat Rat, neonatal Neurons, superior cervical ganglia Trypsin: 0.1%
 Basal L-15 medium Mains, R., and Patterson, P.: Primary Cultures of Dissociated Sympathetic Neurons I. Establishment of Long-Term Growth in Culture and Studies of Differentiated Properties, J Cell Biol 59, 329, 1973
Salamander Salamander, 18-25 cm Retina Papain: 14 u/ml
 Saline Townes-Anderson, E., MacLeish, P., and Raviola, E.: Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase, J Cell Biol 100, 175, 1985
Salamander Salamander (A.tigrinum) Photoreceptors, retina Papain: 0.05%
 (see reference) Bader, C., MacLeish, P., and Schwartz, E.: Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina, Proc Natl Acad Sci U S A 75, 3507, 1978
Sheep Lamb
(also calf)		 Oligodendroglia
Neural		 Trypsin: 0.1%
 (see reference) Poduslo, S., Miller, K., and McKhann, G.: Metabolic Properties of Maintained Oligodendroglia Purified from Brain, J Biol Chem 253, 1592, 1978
Shellfish Snails (Helisoma trivolis), albino, adult Buccal ganglia; SLT muscle Trypsin: 0.2%
 DMEM Zoran, M., Doyle, R. and Haydon, P.: Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis, Neuron 6, 145, 1991
Shellfish Pond snail (Helisoma) albino, adult Somata, buccal ganglia Trypsin: 0.2%
 Antibiotic saline, Leibowitz 50% Haydon, P.: The Formation of Chemical Synapses Between Cell-Cultured Neuronal Somata, J Neurosci 8, 1032, 1988
Shellfish Helisoma trivovlis Buccal ganglia Trypsin: 0.2%
 Liebowitz L-15 medium Cohan, C., Haydon, P., and Kater, S.: Single Channel Activity Differs in Growing and Nongrowing Growth Cones of Isolated Identified Neurons of Helisoma, J Neurosci Res 13, 285, 1985
Shellfish Mollusc, juvenile 1-2 gm or adult 50-100 gm Neurons
LUQ cells		 Protease: 1%
 L15 medium Schacher, S., and Proshanksy, E.: Neurite Regeneration by Aplysia Neurons in Dissociated Cell Culture: Modulation by Aplysia Hemolymph and the Presence of the Initial Axonal Segment, J Neurosci 3 (12), 2403, 1983
Shellfish Aplysia californica LUQ cells
RUQ cells		 Protease: 1%
 L15 medium Camardo, J., Proshansky, E., and Schacher, S.: Identified Aplysia Neurons Form Specific Chemical Synapses in Culture, J Neurosci 3 (12), 2614, 1983
Turtle Turtle (Pseudemys scripta elegans) Retinal Papain: 0.1% (13.5 u/mg)
 Kreb's Ringer Lam, D.: Biosynthesis of Acetylcholine in Turtle Photoreceptors, Proc Natl Acad Sci U S A 69, 1987, 1972