Neural - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Avian Quail and Chicken, embryonic Midgut DRG Collagenase animal free: 0.05%
Neutral Protease: 0.5%
Ham's F12 Rollo, B., Zhang, D., Simkin, J., Menheniott, T. and Newgreen, D.: Why are Enteric Ganglia so Small? Role of Differential Adhesion of Enteric Neurons and Enteric Neural Crest Cells., F1000Res 4, 113, 2015
Avian Chick, White Leghorn, embryos Cilary ganglion neurons Collagenase Type 1: 0.1%
PBS Temburni, M., Rosenberg, M., Pathak, N., McConnell, R. and Jacob, M.: Neuronal Nicotinic Synapse Assembly Requires the Adenomatous Polyposis Coli Tumor Suppressor Protein., J Neurosci 24, 6776, 2004
Avian Chicken and Quail Shikano T: [Correlation of karyotype with clinical features in childhood acute lymphoblastic leukemia].,Hokkaido Igaku Zasshi Vol. 64,, , 727-37, 1989
Bovine Bovine
(also rat)
Adrenal chromaffin
Trypsin: 0.06%
25mM HEPES buffered Locke's solution, CMF Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990
Bovine Bovine Microvascular endothelial Collagenase/Dispase: 0.1%
MEM Bowman, P., Betz, A., and Goldstein, G.: Primary Culture of Microvascular Endothelial Cells From Bovine Retina, In Vitro 18 (7), 626, 1982
Bovine Calf
(also lamb)
Trypsin: 0.1%
(see reference) Poduslo, S., Miller, K., and McKhann, G.: Metabolic Properties of Maintained Oligodendroglia Purified from Brain, J Biol Chem 253, 1592, 1978
Chicken Chicken, White Leghorn, embryos, 17-21 day Neurons Papain: 40 u/ml
HEPES Raman, I., and Trussell, L.: The Kinetics of the Response to Glutamate and Kainte in Neurons of the Avian and Cochlear Nucleus, Neuron 9, 173, 1992
Chicken Chick, embryo, 10-14 day Flat, retina Trypsin: 0.1%
Tyrode's solution, CMF Moyer, M., Bullrich, F., and Sheffield, J.: Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina, In Vitro Cell Dev Biol 26, 1073, 1990
Chicken Chick, White Leghorn, embryos Cerebral neurons Trypsin: Trypsin: 0.125-0.25%
DMEM Coates, P., and Nathan, R.: Feasibility of Electrical Recordings From Unconnected Vertebrate CNS Neurons Cultured in a Three-Dimensional Extracellular Matrix, J Neurosci Methods 20, 203, 1987
Chicken Chick, White Leghorn, embryo
(also rat, SD, embryo)
Spinal cord Trypsin: 0.05%
Phosphate buffer
(see reference)
Schnaar, R., and Schaffner, A.: Separation of cell types from embryonic chicken and rat spinal cord: characterization of motoneuron-enriched fractions, J Neurosci 1, 204, 1981
Chicken Chick Dorsal root ganglion neurons
Spinal cord
Trypsin: 0.1%
Puck's saline, CMF Choi, D., and Fiscbach, G.: GABA Conductance of Chick Spinal Cord and Dorsal Root Ganglion Neurons in Cell Culture, J Neurophysiol 45, 605, 1981
Chicken Chick, White Leghorn embryo, 8 day Neurons, ganglia Trypsin: 0.25%
HBSS, CMF Bottenstein, J., Skaper, S., Varon, S., and Sato, G.: Selective Survival of Neurons from Chick Embryo Sensory Ganglionic Dissociates Utilizing Serum-Free Supplemented Medium, Exp Cell Res 125, 183, 1980
Chicken Chicken, White Leghorn, fertile eggs Ciliary ganglion neurons Trypsin: 0.25%
Eagle's MEM Tuttle, J., Suszkiw, J., and Ard, M.: Long-Term Survival and Development of Dissociated Parasympathetic Neurons in Culture, Brain Res 183, 161, 1980
Chicken Chicken, embryos, 9-10 days old Dorsal root ganglia neurons Collagenase: 0.01%
Eagle's MEM Mudge, A., Leeman, S., and Fischbach, G.: Enkephalin Inhibits Release of Substance P From Sensory Neurons in Culture and Decreases Action Potential Duration, Proc Natl Acad Sci U S A 76 (1), 526, 1979
Chicken Chick embryos Ganglion chains, sympathetic ganglia Trypsin: 0.25%
Krebs Phosphosaline McCarthy, K., and Partlow, L.: Preparation of Pure Neuronal and Non-Neuronal Cultures From Embryonic Chick Sympathetic Ganglia: A New Method Based on Both Differential Cell Adhesiveness and the Formation of Homotypic Neuronal Aggregates, Brain Res 114, 391, 1976
Chicken Chick, White Leghorn, embryos, 7 day Neural retina Collagenase: 0.25%
Elastase: 0.2%
Hyaluronidase: 1.0%
Papain: 1.0%
Protease: 0.1%
Trypsin: 0.05%
HBSS Wiseman, L., and Hammond, W.: The Reacquisition of Cell Adhesiveness Following Tissue Disaggregation by Eleven Different Agents, J Exp Zool 197, 429, 1976
Fish Zebrafish, embryonic Motorneurons PDS kit: per instructions
L-15 Spiro, Z., Koh, A., Tay, S., See, K. and Winkler, C.: Transcriptional Enhancement of Smn Levels in Motoneurons is Crucial for Proper Axon Morphology in Zebrafish., Sci Rep 6, 27470, 2016
Fish Fish, zebrafish Motor neurons Collagenase Type 2: 0.1%
See reference Sakowski, S., Lunn, J., Busta, A., Palmer, M., Dowling, J. and Feldman, E.: A Novel Approach to Study Motor Neurons from Zebrafish Embryos and Larvae in Culture., J Neurosci Methods 205, 277-82, 2012
Fish Zebrafish, Dania rerio, embryo Rohon-Beard neurons Trypsin: 0.2%
L-15/Hepes Won, Y., Ono, F. and Ikeda, S.: Identification and Modulation of Voltage-Gated Ca2+ Currents in Zebrafish Rohon-Beard Neurons., J Neurophysiol 105, 442-53, 2011
Fish Zebrafish, Danio rerio, embryo Neurons PDS kit: with modifications
EBSS Cerda, G., Hargrave, M. and Lewis, K.: RNA Profiling of FAC-sorted Neurons from the Developing Zebrafish Spinal Cord., Dev Dyn 238, 150-61, 2009
Fish Black ghose knige fish, adult (Apteronotus albifrons) Neurons, spinal cord Trypsin: 0.4%
PBS, CMF Anderson, M.J.: Differences in Growth of Neurons from Normal and Regenerated Teleost Spinal Cord in vitro, In Vitro Cell Dev Biol 29A, 145, 1993
Frog Xenopus, embryonic Neuron Collagenase Type 1: 0.1%
Steinberg's solution Takahashi, T., Nakajima, Y., Hirosawa, K., Nakajima, S., and Onodera, K.: Structure and Physiology of Developing Neuromuscular Synapses in Culture, J Neurosci 7, 473, 1987
Guinea-Pig Guinea-pig, newborn Neuron, enteric Trypsin: 0.125%
Medium 199 Jessen, K, McConnell, J., Purves, R., Burnstock, G., and Chamley-Campbell, J.: Tissue Culture of Mammalian Enteric Neurons, Brain Res 152, 573, 1978
Hamster Hamster, male Vomeronasal organ neurons Collagenase Type 1: 0.02%
Trypsin: 0.02%
PBS Liman ER: Regulation by voltage and adenine nucleotides of a Ca2+-activated cation channel from hamster vomeronasal sensory neurons, J Physiol 548, 777, 2003
Human Human Spinal Cord Neural Progenitor PDS kit: with modifications
EBSS Mothe, A. and Tator, C.: Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord., J Vis Exp , e52732, 2015
Human Human Neural, various Papain: 12 u/ml
Trypsin: see reference
Collagenase/Dispase: see reference
see reference Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S: Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24, Stem Cells 25, 1560-70, 2007
Human Human, 9 month Neurons Collagenase Type 4: 1.33%
Papain: 0.07 u/ml
Neutral Protease: 1 mg/ml
DMEM/F12 Dietrich J, Lacagnina M, Gass D, Richfield E, Mayer-Proschel M, Noble M, Torres C, Proschel C.: EIF2B5 mutations compromise GFAP+ astrocyte generation in vanishing white matter leukodystrophy, Nat Med 11, 277, 2005
Human Human, 26 week Neural progenitor cells Papain: 2.5 u/ml
Deoxyribonuclease I: 250 u/ml
Neutral Protease: 1 u/ml
DMEM/F-12 Fuja TJ, Schwartz PH, Darcy D, Bryant PJ.: Asymmetric localization of LGN but not AGS3, two homologs of Drosophila pins, in dividing human neural progenitor cells, J Neurosci Res 75, 782, 2004
Human Human, adult Ventricular epithelial Papain: 11.4 u/ml
Deoxyribonuclease I: 10 u/ml
DMEM/F12 Roy NS, Benraiss A, Wang S, Fraser RA, Goodman R, Couldwell WT, Nedergaard M, Kawaguchi A, Okano H, Goldman SA: Promoter-targeted selection and isolation of neural progenitor cells from the adult human ventricular zone, J Neurosci Res 59, 321, 2000
Human Human, 5-65 years Retinal pigment epithelial (RPE) Trypsin: 0.25%
HBSS Von Recum, H., Okano, T., Kim, S, and Bernstein, P.: Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture, Exp Eye Res 69, 97, 1999
Human Human Dorsal root ganglion neurons Collagenase Type 1: 0.2%
Neutral Protease: 0.5%
DMEM/F-12 Dib-Hajj SD, Tyrrell L, Cummins TR, Black JA, Wood PM, Waxman SG.: Two tetrodotoxin-resistant sodium channels in human dorsal root ganglion neurons, F.E.B.S. Lett. 462, 117, 1999
Insect Drosophila, larval Neuroblasts Collagenase Type 1: 0.1%
Papain: 0.1%
Rinaldini solution Berger, C., Harzer, H., Burkard, T., Steinmann, J., van der Horst, S., Laurenson, A., Novatchkova, M., Reichert, H. and Knoblich, J.: FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal., Cell Rep 2, 407, 2012
Insect Drosophila Dendrites Collagenase: 0.05%
Neutral Protease: 0.2%
HBSS Sanchez-Soriano, N., Bottenberg, W., Fiala, A., Haessler, U., Kerassoviti, A., Knust, E., Lohr, R. and Prokop, A.: Are Dendrites in Drosophila Homologous to Vertebrate Dendrites?, Dev Biol 288, 126, 2005
Insect Gryllus Bimaculatus Giant interneurons Collagenase: 0.05%
Neutral Protease: 0.2%
Leibovitz's L15 Kloppenburg, P. and Horner, M.: Voltage-Activated Currents in Identified Giant Interneurons Isolated from Adult Crickets Gryllus Bimaculatus, J Exp Biol 201 (Pt 17), 2529, 1998
Mouse Mouse, 2-3 month Sympathetic neurons Papain: 10 u/ml
Collagenase Type 2: 0.13%
Neutral Protease: 0.22%
EBSS Sun, H., Tsai, W., Li, B., Tao, W., Chen, P. and Rubart, M.: Voltage-Induced Calcium Release in Postganglionic Sympathetic Neurons in Adult Mice., PLoS ONE 11, e0148962, 2016
Mouse Mouse Spinal motor neurons, astrocytes, microglia Papain: 0.2%
HBSS Beaudet, M., Yang, Q., Cadau, S., Blais, M., Bellenfant, S., Gros-Louis, F. and Berthod, F.: High Yield Extraction of Pure Spinal Motor Neurons, Astrocytes and Microglia from Single Embryo and Adult Mouse Spinal Cord., Sci Rep 5, 16763, 2015
Mouse Mouse DRG neurons Collagenase Type 2: 0.1%
Trypsin: 0.25%
DMEM/F-12 Lee, B., Cho, H., Jung, J., Yang, Y., Yang, D. and Oh, U.: Anoctamin 1 Contributes to Inflammatory and Nerve-Injury Induced Hypersensitivity., Mol Pain 10, 5, 2014
Mouse Mouse, neonatal Schwann cells Collagenase: 0.05-0.1%
Trypsin: 0.125-0.25%
DMEM Stettner, M., Lohmann, B., Wolffram, K., Weinberger, J., Dehmel, T., Hartung, H., Mausberg, A. and Kieseier, B.: Interleukin-17 Impedes Schwann Cell-Mediated Myelination., J Neuroinflammation 11, 63, 2014
Mouse Mouse Oligodendrocytes Papain: 0.1%
Deoxyribonuclease I: 0.0002%
HBSS Harris, M., Hulseberg, P., Ling, C., Karman, J., Clarkson, B., Harding, J., Zhang, M., Sandor, A., Christensen, K., Nagy, A., Sandor, Matyas,S. and Fabry, Z.: Immune Privilege of the CNS is not the Consequence of Limited Antigen Sampling., Sci Rep 4, 4422, 2014
Mouse Mouse hippocampal neurons PDS kit: per instructions
DMEM Williams, E., Zhong, X., Mohamed, A., Li, R., Liu, Y., Dong, Q., Ananiev, G., Mok, J., Lin, B., Lu, J., Chiao, C., Cherney, R., Li, H., Zhang, S. and Chang, Q: Mutant Astrocytes Differentiated from Rett Syndrome Patients-Specific iPSCs Have Adverse Effects on Wild-Type Neurons., Hum Mol Genet 23, 2968-80, 2014
Mouse Mouse Neurosperes Papain: 0.1%
Deoxyribonuclease I: 0.1%
NeuroCult NSC Stuart, M., Corrigan, F. and Baune, B.: Knockout of CXCR5 Increases the Population of Immature Neural Cells and Decreases Proliferation in the Hippocampal Dentate Gyrus., J Neuroinflammation 11, 31, 2014
Mouse Mouse, 4-6 week Schwann Collagenase Type 1: 0.01%
Trypsin: 0.125%
DMEM Masaki, T., Qu, J., Cholewa-Waclaw, J., Burr, K., Raaum, R. and Rambukkana, A.: Reprogramming Adult Schwann Cells to Stem Cell-Like Cells by Leprosy Bacilli Promotes Dissemination of Infection., Cell 152, 51, 2013
Mouse Mouse Oligodendrocyte precursor cells Papain: 0.09%
DMEM Medina-Rodriguez, E., Arenzana, F., Bribian, A. and de Castro, F.: Protocol to Isolate a Large Amount of Functional Oligodendrocyte Precursor Cells from the Cerebral Cortex of Adult Mice and Humans., PLoS ONE 8, e81620, 2013
Mouse Mouse Enteric neurons Collagenase Type 4: 0.1%
Trypsin: 0.05%
Deoxyribonuclease I: 400 u/ml
DMEM/F12 Zhang, Y. and Hu, W.: Mouse Enteric Neuronal Cell Culture., Methods Mol Biol 1078, 55-63, 2013
Mouse Mouse, 6-8 week Neural precursors Trypsin: 0.13%
Hyaluronidase: 0.08%
see reference Babona-Pilipos, R., Popovic, M. and Morshead, C.: A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field., J Vis Exp 68, e4193, 2012
Mouse Mouse, adult Retinal neurons Papain: 10-20 ul
HBSS Goetz, J. and Trimarchi, J.: Single-Cell Profiling of Developing and Mature Retinal Neurons., J Vis Exp 62, e3824, 2012
Mouse Mouse Neurons Papain: 100 ul
DMEM Li, Y., Roy, B., Wang, W., Zhang, L., Sampson, S. and Lin, D.: Imaging pHluorin-Tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons., J Vis Exp 69, e4450, 2012
Mouse Mouse, P4-5 Neuroshere Trypsin: 0.25%
Papain: 100 u
Deoxyribonuclease I: 0.025%
Pro-N Ziegler, A., Schneider, J., Qin, M., Tyler, W., Pintar, J., Fraidenraich, D., Wood, T and Levison, S.: IGF-II Promotes Stemness of Neural Restricted Precursors., Stem Cells 30, 1265, 2012
Mouse Mouse, 6-8 week Neural stem cells Papain: 0.01%
Neutral Protease: 0.1%
Deoxyribonuclease I: 0.01%
DMEM/F12 Bracko, O., Singer, T., Aigner, S., Knobloch, M., Winner, B., Ray, J., Clemenson, G., Suh, H., Couillard-Despres, S., Aigner, L., Gage, F. and Jessberger, S.: Gene Expression Profiling of Neural Stem Cells and their Neuronal Progeny Reveals IGF2 as a Regulator of Adult Hippocampal Neurogenesis., J Neurosci 32, 3376-87, 2012
Mouse Mouse Nerve Progenitors Collagenase Type 4: 0.025%
Trypsin NF 1:250: .025%
HBSS Salisbury, E., Lazard, Z., Ubogu, E., Davis, A. and Olmsted-Davis, E.: Transient Brown Adipocyte-Like Cells Derive from Peripheral Nerve Progenitors in Response to Bone Morphogenetic Protein 2., Stem Cells Transl Med 1, 874-85, 2012
Mouse Mouse, embryonic Neural crest Collagenase/Dispase: 0.1%
DMEM Pfaltzgraff, E., Mundell, N. and Labosky, P.: Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube., J Vis Exp , e4134, 2012
Mouse Mouse, embryo, 14 day Spinal cord neurons Papain: 0.05%
Deoxyribonuclease I: 0.004%
PBS/DMEM Pollari, E., Savchenko, E., Jaronen, M., Kanninen, K., Malm, T., Wojciechowski, S., Ahtoniemi, T., Goldsteins, G., Giniatullina, R., Giniatullin, R., Koistinaho, J. and Magga, J.: Granulocyte Colony Stimulating Factor Attenuates Inflammation in a Mouse Model of Amyotrophic Lateral Sclerosis., J Neuroinflammation 8, 74, 2011
Mouse Mouse, 1-2 day Oligodendrocytes, dorsal root ganglia Papain: 0.15%
Deoxyribonuclease I: 0.006%
DMEM O'Meara, R., Ryan, S., Colognato, H. and Kothary, R.: Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-Cultures from Post-Natal Murine Tissues., J Vis Exp 54, 3324, 2011
Mouse Mouse, embryonic Motorneurons Trypsin: 0.025%
HBSS Conrad, R., Jablonka, S., Sczepan, T., Sendtner, M., Wiese, S. and Klausmeyer, A.: Lectin-Based Isolation and Culture of Mouse Embryonic Motoneurons., J Vis Exp 55, e3200, 2011
Mouse Mouse, 4-8 week Olfactory ensheathing Collagenase: 0.15%
Papain: 12 u/ml
DMEM Radtke, C., Sasaki, M., Lankford, K., Gallo, V. and Kocsis, J.: CNPase Expression in Olfactory Ensheathing Cells., J Biomed Biotechnol 2011, 608496, 2011
Mouse Mouse Dentate Gyrus Neural Precursor Papain: 2.5 u/ml
Neutral Protease: 1.0 u/ml
Deoxyribonuclease I: 250 u/ml
Neurobasal A Babu, H., Claasen, J., Kannan, S., Runker, A., Palmer, T. and Kempermann, G.: A Protocol for Isolation and Enriched Monolayer Cultivation of Neural Precursor Cells from Mouse Dentate Gyrus., Front Neurosci 5, 89, 2011
Mouse Mouse, 30 day Motoneurons Papain: 0.2%
Hibernate A Milligan, C. and Gifondorwa, D.: Isolation and Culture of Postnatal Spinal Motoneurons., Methods Mol Biol 793, 77, 2011
Mouse Mouse Hippocampal and retinal neurons Papain: 1%
Deoxyribonuclease I: 5 u/ml
HBSS Brown, J., Gianino, S. and Gutmann, D.: Defective cAMP Generation Underlies the Sensitivity of CNS Neurons to Neurofibromatosis-1 Heterozygosity, J Neurosci 30, 5579, 2010
Mouse Mouse CNS leukocytes Collagenase Type 4: 300 u/ml
HBSS Sayed, B., Christy, A., Walker, M. and Brown, M.: Meningeal Mast Cells Affect Early T Cell Central Nervous System Infiltration and Blood-Brain Barrier Integrity Through TNF: a Role for Neutrophil Recruitment?, J Immunol 184, 6891-900, 2010
Mouse Mouse, adult Spinal microganglia Papain: 0.2%
Hibernate A Yip, P., Kaan, T., Fenesan, D. and Malcangio, M.: Rapid Isolation and Culture of Primary Microglia from Adult Mouse Spinal Cord., J Neurosci Methods 183, 223-37, 2009
Mouse Mouse, embryonic Dopaminergic neurons Trypsin: 0.1%
Deoxyribonuclease I: 0.02%
DMEM Radad Khaled, Gille Gabriele, Rausch Wolf-Dieter: Dopaminergic neurons are preferentially sensitive to long-term rotenone toxicity in primary cell culture, Toxicol In Vitro 22, 68-74, 2008
Mouse Mouse, neonatal DRG neurons Collagenase: 0.2%
Trypsin: 0.05%
Ham's F12 Pedrola, L., Espert, A., Valdes-Sanchez, T., Sanchez-Piris, M., Sirkowski, E., Scherer, S., Farinas, I., and Palau, F.: Cell Expression of GDAP1 in the Nervous System and Pathogenesis of Charcot-Marie-Tooth Type 4A Disease., J Cell Mol Med 12, 679, 2008
Mouse Mouse, 6 mo Neurons, neurospheres Papain: 0.2%
Hibernate Brewer Gregory J, Torricelli John R: Isolation and culture of adult neurons and neurospheres, Nat Protoc 2, 1490-8, 2007
Mouse Mouse Neural, various Papain: 12 u/ml
Trypsin: see reference
Collagenase/Dispase: see reference
see reference Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S: Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24, Stem Cells 25, 1560-70, 2007
Mouse Mouse, 6-8 week Sensory neurons, DRG Papain: 20 u/ml
Collagenase Type 2: 0.4%
Neutral Protease: 0.46%
HBSS Malin, S., Davis, B. and Molliver, D.: Production of Dissociated Sensory Neuron Cultures and Considerations for their use in Studying Neuronal Function and Plasticity, Nat Protoc 2, 152, 2007
Mouse Mouse, C57BL/6, 1 year Neurons Papain: 0.2%
DMEM Eide, L, and McMurray, C: Culture of Adult Mouse Neurons, Biotechniques 38(1), 99-104, 2005
Mouse Mouse, adult Brain and spinal cord cells Trypsin: 0.25%
PBS Gonzalez John M, Bergmann Cornelia C, Fuss Babette, Hinton David R, Kangas Cindy, Macklin Wendy B, Stohlman Stephen A: Expression of a dominant negative IFN-gammareceptor on mouse oligodendrocytes, Glia 51, 22-34, 2005
Mouse Mouse, neonatal Neurons PDS kit: per instructions
EBSS Gill JC, Moenter SM, Tsai PS: Developmental regulation of gonadotropin-releasing hormone neurons by fibroblast growth factor signaling, Endocrinology 145, 3830, 2004
Mouse Mouse, C57BL Cerebellar granule cell precursors Papain: 16.5 u/ml
Deoxyribonuclease I: 0.008%
Dulbecco's PBS Okano-Uchida T, Himi T, Komiya Y, and Ishizaki Y: Cerebellar granule cell precursors can differentiate into astroglial cells, Proc Natl Acad Sci U S A 101, 1211, 2004
Mouse Mouse, 1-3 day Neurons, ganglia Papain: 20 u/ml
Deoxyribonuclease I: 100 u/ml
Collagenase: 0.3%
Trypsin: 0.05%
HBSS Savchenko V, Sung U, Blakely RD.: Cell surface trafficking of the antidepressant-sensitive norepinephrine transporter revealed with an ectodomain antibody, Mol Cell Neurosci 24, 1131, 2003
Mouse Mouse, male Trigeminal senesory neurons Papain: 20 u/ml
HEPES buffered saline Roberts, L., MacDonald, C.and Mark, C.: Anandamide is a Partial Agonist at Native Vanilloid Receptors in Acutely Isolated Mouse Trigeminal Sensory Neurons., Br J Pharmacol 137, 421, 2002
Mouse Mouse, postnatal-day-1-old (P1) Neurons Trypsin: 0.25%
NGF-containing medium Deshmukh, M., Kuida, K., and Johnson Jr., E.: Caspase Inhibition Extends the Commitment to Neuronal Death Beyond Cytochrome c Release to the Point of Mitochondrial Depolarization, J Cell Biol 150 (1), 131, 2000
Mouse Mouse embryos Neurons, DRG and SCG Trypsin: 0.25%
L-15 medium Lee, K., Davies, A., and Jaenisch, R.: P75-Eficient Embryonic Dorsal Root Sensory and Neonatal Sympathetic Neurons Display a Decreased Sensitivity to NGF, Development 120, 1027, 1994
Mouse mouse, 65 days Neurons, neuronal precursors Trypsin: 0.1%
Deoxyribonuclease I: 0.001%
DMEM Richards L., Kilpatrick T., and Bartlett P.: De Novo Generation of Neuronal Cells from the Adult Mouse Brain, Proc Natl Acad Sci U S A 89, 8591, 1992
Mouse Mouse, CD-1, neonate Neurons, dorsal root ganglion Trypsin: 0.25%
HBSS Quinn, S. and De Boni, U.: Enhanced Neuronal Regeneration by Retinoic Acid of Murine Dorsal Root Ganglia and of Fetal Murine and human Spinal Cord in vitro, In Vitro Cell Dev Biol 27, 55, 1991
Mouse Mouse, fetal Precursor Trypsin: 0.5%
PBS Kitani, H., Shiurba, R., Sakakura, T., Tomooka, Y.: Isolation and Characterization Of Mouse Neural Precursor Cells in Primary Culture, In Vitro Cell Dev Biol 27, 615, 1991
Mouse Mouse (SWR or CF1), 1-3 months Papillae, taste receptor Pronase E: 0.15%
Carbonate-Phosphate buffer
(see reference)
Spielman, A., Mody, I., Brand, J., Whitney, G., MacDonald, J., and Salter, M.: A Method for Isolating and Patch-Clamping Single Mammalian Taste Receptor Cells, Brain Res 503, 326, 1989
Mouse Mouse, neonatal
(also chick)
PNS test neurons Trypsin: 0.08%
Eagle's Basal Medium (see reference) Varon, S., Skaper, S., Barbin, G., Selak, I., and Manthorpe, M.: Low Molecular Weight Agents Support Survival of Cultured Neurons From the Central Nervous System, J Neurosci 4 (3), 654, 1984
Mouse Mouse (BALB/c), adult Neurons, spinal cord Collagenase Type 3: 0.25%
Hank's BSS, CMF Eagleson, K. and Bennett, M.: Survival of Purified Motor Neurons In Vitro: Effects of Skeletal Muscle-Conditioned Medium, Neurosci Lett 38, 187, 1983
Mouse Mouse, 0-30 day Neural Trypsin NF 1:250: 50 0.25%
BSS Shrier, B., Wilson, S., and Nirenberg, M.: Cultured Cell Systems and Methods for Neurobiology, Vol. 32,, , 765, 1974
Ovine Lamb
(also calf)
Trypsin: 0.1%
(see reference) Poduslo, S., Miller, K., and McKhann, G.: Metabolic Properties of Maintained Oligodendroglia Purified from Brain, J Biol Chem 253, 1592, 1978
Porcine Porcine, adult, 60-100 kg Superior cervical ganglia Papain: 2 u/ml
Collagenase: 0.12%
Neutral Protease: 0.48%
HBSS Si ML, Lee TJ.: Presynaptic alpha7-nicotinic acetylcholine receptors mediate nicotine-induced nitric oxidergic neurogenic vasodilation in porcine basilar arteries, J Pharmacol Exp Ther 298, 122, 2001
Quail Quail Neural crest Trypsin: 0.05%
MEM, HBSS Sieber-Blum, M., and Cohen, A.: Clonal Analysis of Quail Neural Crest Cells: They are Pluripotent and Differentiate in Vitro in the Absence of Noncrest Cells, Dev Biol 80, 96, 1980
Rat Rat, SD Schwann Collagenase Type 1: 0.05%
DMEM Schuh, C., Hercher, D., Stainer, M., Hopf, R., Teuschl, A., Schmidhammer, R. and Redl, H.: Extracorporeal Shockwave Treatment: A Novel Tool to Improve Schwann Cell Isolation and Culture., Cytotherapy 18, 760-70, 2016
Rat Rat, embryonic and neonatal Cortical neurons, DRG PDS kit: per instructions
Collagenase Type 1: 0.17%
Neurobasal Rheaume, C., Cai, B., Wang, J., Fernandez-Salas, E., Aoki, K., Francis, J. and Broide, R.: A Highly Specific Monoclonal Antibody for Botulinum Neurotoxin Type A-Cleaved SNAP25., Toxins 7, 2354-70, 2015
Rat Rat, Embryonic Cerebral neurons Papain: 2 u/ml
Deoxyribonuclease I: 0.01%
DMEM-PBS Oyanagi, K., Tashiro, T. and Negishi, T.: Cell-Type-Specific and Differentiation-Status-Dependent Variations in Cytotoxicity of Tributyltin in Cultured Rat Cerebral Neurons and Astrocytes., J Toxicol Sci 40, 459-68, 2015
Rat Rat, SD, 6-9 week Dorsal Root Ganglia Collagenase Type 3: 0.2%
HBSS Murayama, C., Watanabe, S., Nakamura, M. and, Norimoto, H.: Inhibitory Activity of Yokukansankachimpihange against Nerve Growth Factor-Induced Neurite Growth in Cultured Rat Dorsal Root Ganglion Neurons., Molecules 20, 14959-69, 2015
Rat Rat Dorsal root ganglion neurons Collagenase Type 2: 0.15%
HBSS Yu, R., Seymour, V., Berecki, G., Jia, X., Akca, M., Adams, D., Kaas, Q. and Craik, D.: Less is More: Design of a Highly Stable Disulfide-Deleted Mutant of Analgesic Cyclic α-Conotoxin Vc1.1., Sci Rep 5, 13264, 2015
Rat Rat, 6-8 week Spinal Cord Neural Progenitor PDS kit: with modifications
EBSS Mothe, A. and Tator, C.: Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord., J Vis Exp , e52732, 2015
Rat Rat, SD Trigeminal ganglion Collagenase Type 2: 0.1%
Papain: 20 u/ml
DMEM/F12 Wei, X., Yan, J., Tillu, D., Asiedu, M., Weinstein, N., Melemedjian, O., Price, T. and Dussor, G.: Meningeal Norepinephrine Produces Headache Behaviors in Rats via Actions Both on Dural Afferents and Fibroblasts.,Cephalalgia Vol. 35,, , 1054-64, 2015
Rat Rat, neonatal Schwann cells Collagenase: 0.05-0.1%
Trypsin: 0.125-0.25%
DMEM Stettner, M., Lohmann, B., Wolffram, K., Weinberger, J., Dehmel, T., Hartung, H., Mausberg, A. and Kieseier, B.: Interleukin-17 Impedes Schwann Cell-Mediated Myelination., J Neuroinflammation 11, 63, 2014
Rat Rat, 1-3 day Hippocampal Neurons Papain: 25 u/ml
L-15 Thurner, P., Gsandtner, I., Kudlacek, O., Choquet, D., Nanoff, C., Freissmuth, M. and Zezula, J.: A Two-State Model for the Diffusion of the A2A Adenosine Receptor in Hippocampal Neurons: Agonist-Induced Switch to Slow Mobility is Modified by Synapse-Associated Protein 102 (SAP102)., J Biol Chem 289, 9263-74, 2014
Rat Rat, SD, embryonic Hypothalamic neuronal PDS kit: per instructions
Neurobasal A Loktev, A. and Jackson, P.: Neuropeptide Y Family Receptors Traffic via the Bardet-Biedl Syndrome Pathway to Signal in Neuronal Primary Cilia., Cell Rep 5, 1316-29, 2013
Rat Rat, E18 Hippocampal Neurons Papain: 0.2%
Hibernate Todd, G., Boosalis, C., Burzycki, A., Steinman, M., Hester, L., Shuster, P. and Patterson, R.: Towards Neuronal Organoids: A Method for Long-Term Culturing of High-Density Hippocampal Neurons., PLoS ONE 8, e58996, 2013
Rat Rat, SD, 175-200 g Trigeminal ganglia Papain: 20 u/ml
Collagenase Type 2: 0.3%
HBSS Yan, J., Melemedjian, O., Price, T. and Dussor, G.: Sensitization of Dural Afferents Underlies Migraine-Related Behavior Following Meningeal Application of Interleukin-6 (IL-6)., Mol Pain 8, 6, 2012
Rat Rat, E19-21 and P1-3 Sympathetic neurons Collagenase Type 2: 0.1%
Neutral Protease: 0.5%
DMEM/F-12 Ghogha, A., Bruun, D. and Lein, P.: Inducing Dendritic Growth in Cultured Sympathetic Neurons., J Vis Exp 61, e3546, 2012
Rat Rat, 20-30 day Dorsal Root Ganglion Papain: 20 u/ml
Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
L-15 Bosmans, F., Puopolo, M., Martin-Eauclaire, M., Bean, B., Swartz, K.: Functional Properties and Toxin Pharmacology of a Dorsal Root Ganglion Sodium Channel Viewed through its Voltage Sensors., J Gen Physiol 138, 59, 2011
Rat Rat, Wistar, E18 Neurons Papain: 0.05%
Neurobasal Giacomello, M., Girardi, S., Scorzeto, M., Peruffo, A., Maschietto, M., Cozzi, B. and Vassanelli, S.: Stimulation of Ca²+ Signals in Neurons by Electrically Coupled Electrolyte-Oxide-Semiconductor Capacitors., J Neurosci Methods 198, 1, 2011
Rat Rat, SD, 200-300 g Dorsal root ganglia, fibroblast Collagenase: 0.125%
DMEM East, E., de Oliveira, D., Golding, J. and Phillips, J.: Alignment of Astrocytes Increases Neuronal Growth in Three-Dimensional Collagen Gels and is Maintained Following Plastic Compression to Form a Spinal Cord Repair Conduit., Tissue Eng Part A 16, 3173, 2010
Rat Rat, Fisher, 7-21 month Hippocampal neurons Papain: 0.2%
Hibernate A Chen, N., Newcomb, J., Garbuzova-Davis, S., Davis Sanberg, C., Sanberg, P. and Willing, A.: Human Umbilical Cord Blood Cells Have Trophic Effects on Young and Aging Hippocampal Neurons in Vitro., Aging Dis 1, 173, 2010
Rat Rat, SD, E18 Neuronal Papain: 0.2%
Neurobasal E Peltier, D., Simms, A., Farmer, J and Miller, D.: Human Neuronal Cells Possess Functional Cytoplasmic and TLR-Mediated Innate Immune Pathways Influenced by Phosphatidylinositol-3-Kinase Signaling, J Immunol 184, 7010, 2010
Rat Rat, SD, 7 day Cerebellar granule neurons PDS kit: per instructions
PBS Tanaka, S., Shaikh, I., Chiocca, E. and Saeki, Y.: The Gs-Linked Receptor GPR3 Inhibits the Proliferation of Cerebellar Granule Cells During Postnatal Development., PLoS ONE 4, e5922, 2009
Rat Rat, SD, embryonic day 18 Hippocampal neurons Papain: 20 u/ml
Neurobasal/B27 Liu, Y., Yohrling, G., Wang, Y., Hutchinson, T., Brenneman, D., Flores, C., Zhao, B.: Carisbamate, a Novel Neuromodulator, Inhibits Voltage-Gated Sodium Channels and Action Potential Firing of Rat Hippocampal Neurons., Epilepsy Res 83, 66, 2009
Rat Rat, embryonic Hypothalamic neurons and glias Papain: 0.5 u/ml
DMEM Yokosuka Makoto, Ohtani-Kaneko Ritsuko, Yamashita Kayoko, Muraoka Daisuke, Kuroda Yoichiro, Watanabe Chiho: Estrogen and environmental estrogenic chemicals exert developmental effects on rat hypothalamic neurons and glias, Toxicol In Vitro 22, 1-9, 2008
Rat Rat, Wistar, 7 day Superior cervical ganglion Collagenase: 0.05%
L-15 Sakisaka, T., Yamamoto, Y., Mochida, S., Nakamura, M., Nishikawa, K., Ishizaki, H., Okamoto-Tanaka, M., Miyoshi, J., Fujiyoshi, Y., Manabe, T. and Takai, Y.: Dual Inhibition of SNARE Complex Formation by Tomosyn Ensures Controlled Neurotransmitter Release., J Cell Biol 183, 323, 2008
Rat Rat, neonatal Dopamine neurons Papain: 20 u/ml
PBS Frank, L., Caldera-Siu, A. and Pothos, E.: Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates., J Vis Exp 21, 820, 2008
Rat Rat, Wistar, 4 day Hippocampal neurons PDS kit: per instructions
Neurobasal A Obradovic Darja, Gronemeyer Hinrich, Lutz Beat, Rein Theo: Cross-talk of vitamin D and glucocorticoids in hippocampal cells, J Neurochem 96, 500-9, 2006
Rat Rat, SD, neonatal astrocytes PDS kit: per instructions
DMEM Lacroix-Fralish, M., Tawfik, V., Nutile-McMenemy, N., Harris, B. and Deleo, J.: Differential Regulation of Neuregulin 1 Expression by Progesterone in Astrocytes and Neurons., Neuron Glia Biol 2, 227, 2006
Rat Rat, 2 month Spinal cord progenitor cells
Hybrid toxins
PDS kit: see reference
Neurobasal A Mothe, A., Kulbatski, I., Van Bendegem, R., Lee, L., Kobayashi, E., Keating, A., and Tator, C.: Analysis of Green Fluorescent Protein Expression in Transgenic Rats for Tracking Transplanted Neural Stem/Progenitor Cells, J Histochem Cytochem 53(10), 1215, 2005
Rat Rat, SD, E19 Dorsal root ganglia PDS kit: per instructions
MEM/Ham's F12 Gavva NR, Tamir R, Qu Y, Klionsky L, Zhang TJ, Immke D, Wang J, Zhu D, Vanderah TW, Porreca F, Doherty EM, Norman MH, Wild KD, Bannon AW, Louis JC, Treanor JJ.: AMG 9810 [(E)-3-(4-t-butylphenyl)-N-(2,3-dihydrobenzo[b][1,4] dioxin-6-yl)acrylamide], a novel vanilloid receptor 1 (TRPV1) antagonist with antihyperalgesic properties, J Pharmacol Exp Ther 313, 474, 2005
Rat Rat, Wistar, E15 Vomeronasal receptor neurons Collagenase/Dispase: 0.1%
Papain: 0.5 u/ml
DMEM/F12 Moriya-Ito K, Osada T, Ishimatsu Y, Muramoto K, Kobayashi T, Ichikawa M: Maturation of vomeronasal receptor neurons in vitro by coculture with accessory olfactory bulb neurons, Chem Senses 30, 111, 2005
Rat Rat, SD, 1-2 day Cortical astrocytes Papain: see reference
DMEM Floyd Candace L, Gorin Fredric A, Lyeth Bruce G: Mechanical strain injury increases intracellular sodium and reverses Na+/Ca2+ exchange in cortical astrocytes, Glia 51, 35-46, 2005
Rat Rat, SD, 0-2 day Superior cervical ganglion Collagenase Type 4: 20 u/ml
Trypsin: 0.25%
DMEM Pedraza Carlos E, Podlesniy Petar, Vidal Noemi­, Arevalo Juan Carlos, Lee Ramee, Hempstead Barbara, Ferrer Isidre, Iglesias Montse, Espinet Carme: Pro-NGF isolated from the human brain affected by Alzheimer's disease induces neuronal apoptosis mediated by p75NTR, Am J Pathol 166, 533-43, 2005
Rat Rat, SD, male, 5-8 week Trigeminal neurons Papain: 20 u/ml
Collagenase: 0.3%
CMF Hanks Connor, M, Naves, L. and McCleskey, E.: Contrasting Phenotypes of Putative Proprioceptive and Nociceptive Trigeminal Neurons Innervating Jaw Muscle in Rat., Mol Pain 1, 31, 2005
Rat Rat, fetal Brainstem and cortical neurons PDS kit: per instructions
DMEM Lovshin JA, Huang Q, Seaberg R, Brubaker PL, Drucker DJ: Extrahypothalamic expression of the glucagon-like peptide-2 receptor is coupled to reduction of glutamate-induced cell death in cultured hippocampal cells, Endocrinology 145, 3495, 2004
Rat Rat, SD, male 150-220 g Dorsal root ganglion neurons Collagenase Type 4: 0.125%
Trypsin: 0.05%
DMEM/Ham's F12 Hu Hong-Zhen, Gu Qihai, Wang Chunbo, Colton Craig K, Tang Jisen, Kinoshita-Kawada Mariko, Lee Lu-Yuan, Wood Jackie D, Zhu Michael X: 2-aminoethoxydiphenyl borate is a common activator of TRPV1, TRPV2, and TRPV3, J Biol Chem 279, 35741-8, 2004
Rat Rat, 15 week Neurons Papain: 0.2%
Hibernate A Evans J, Sumners C, Moore J, Huentelman MJ, Deng J, Gelband CH, and Shaw G: Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem, J Neurophysiol 87, 1076, 2002
Rat Rat, SD, male, 270-330 g Spinal progenitor cells PDS kit: with modifications
Neurobasal medium Lin CR, Wu PC, Shih HC, Cheng JT, Lu CY, Chou AK, Yang LC.: Intrathecal spinal progenitor cell transplantation for the treatment of neuropathic pain, Cell Transplant 11, 17, 2002
Rat Rat, Fisher, 8-9 week Adult progenitor Papain: 2.5 u/ml
Deoxyribonuclease I: 250 u/ml
Neutral Protease: 1 u/ml
DMEM/F-12 Lie DC, Dziewczapolski G, Willhoite AR, Kaspar BK, Shults CW, Gage FH.: The adult substantia nigra contains progenitor cells with neurogenic potential, J Neurosci 22, 6639, 2002
Rat Rat, SD, embryonic Sciatic Nerve and Gut Neural Crest Stem Cells Collagenase Type 4: 0.025%
Trypsin: 0.005%
Deoxyribonuclease I: 0.05%
HBSS Bixby, S., Kruger, G., Mosher, J., Joseph, N. and Morrison, S.: Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity., Neuron 35, 643, 2002
Rat Rat Neurons, hippocampal Papain: 15 - 20 u/ml
Eagle's MEM
(see reference)
Liu, Q., Kawai, H., and Berg, D.: B-Amyloid Peptide Blocks the Response of a7-Containing Nicotinic Receptors on Hippocampal Neurons, PNAS 98 (8), 4734, 2001
Rat Rat, newborn, 7 days old Neurons Trypsin: 0.25%
MEM10 Acosta, C., Fabrega, A., Masco, D., and Lopez, H.: A Sensory Neuron Subpopulation with Unique Sequential Survival Dependence on Nerve Growth Factor and Basic Fibroblast Growth Factor during Development, J Neurosci 21 (22), 8873, 2001
Rat Rat, embryonic 18-19 day Hippocampal neurons PDS kit: see reference
DMEM Mabuchi T, Kitagawa K, Kuwabara K, Takasawa K, Ohtsuki T, Xia Z, Storm D, Yanagihara T, Hori M, Matsumoto M: Phosphorylation of cAMP response element-binding protein in hippocampal neurons as a protective response after exposure to glutamate in vitro and ischemia in vivo, J Neurosci 21, 9204-13, 2001
Rat Rat, embryonic, day 18 Cortical Papain:
Neurobasal medium and DMEM O'Connor, S., Andreadis, J., Shaffer, K., Ma, W., Pancrazio, J., and Stenger, D.: Immobilization of Neural Cells in Three-Dimensional Matrices for Biosensor Applications, Biosensors & Bioelectronics 14, 871, 2000
Rat Rat, Wister, postnatal, P1-3 days Neurons, hippocampal Papain: 20 u/ml
EBSS Neuhoff, H., Roeper, J., Schweizer, M.: Activity-Dependent Formation of Perforated Synapses in Cultured Hippocampal Neurons, Eur J Neurosci 11, 4241, 1999
Rat Rat, SD, female, pregnant Sciatic nerves Trypsin: 0.025%
Collagenase Type 3: 0.1%
L-15 medium
(see reference)
Morrison, S., White, P., Zock, C., and Anderson, D.: Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells, Cell 96, 737, 1999
Rat Rat
(also mice)
Spinal cord Trypsin: 0.133%
HBSS and PIPES Johansson, C., Momma, S., Clarke, D., Risling, M., Lendahl, U., and Frisen, J: Identification of a Neural Stem Cell in the Adult Mammalian Central Nervous System, Cell 96, 25, 1999
Rat Rat, SD, embryonic Sciatic nerves Trypsin: 0.025%
Collagenase Type 3: 0.1%
L-15 medium
(see reference)
Morrison, S., White, P., Zock, C., and Anderson, D.: Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells, Cell 96, 737, 1999
Rat Rat, (Long Evans), 2-5 day old Neurons, hippocampal Papain: 20 u/ml
EBSS Wilding, T., and Huettner, J.: Activation and Desensitization of Hippocampal Kainate Receptors, J Neurosci 17 (8), 2713, 1997
Rat Rat, Wister and SD, newborn, 0-21 days, either sex Myenteric ganglia Trypsin: 0.05%
MEM-HEPES Schafer, K., Saffrey, M., Burnstock, G., and Mastres-Ventura, P.: A New Method for the Isolation of Myenteric Plexus from the Newborn Rat Gastrointestinal Tract, Brain Res Proto 1, 109, 1997
Rat Rat, pups, 24-48 h old Neurons, hippocampal Papain: 20 u/ml
Harvest buffer Hall, R., and Soderling, T.: Differential Surface Expression and Phosphorylation of the N-Methyl-D-Aspartate Receptor NR1 and NR2 in Cultured Hippocampal Neurons, J Biol Chem 272 (7), 4135, 1997
Rat Rat, SD, adult, 250-300g, P8 Neurons, DRG Neutral Protease: 0.5%
L-15 w/ CO2 Davies, S., Fitch, M., Memberg, S., Hall, A, Raisman, G., and Silver, J.: Regeneration of Adult Axons in White Matter Tracts of the Central Nervous System, Nature 390, 680, 1997
Rat Rat, 1-4 day old Neurons, hippocampal Papain: 20 u/ml
MEM Twitchell, W., Brown, S., and Mackie, K.: Cannabinoids Inhibit N- and P/Q-Type Calcium Channels in Cultured Rat Hippocampal Neurons, J Neurophysiol 78, 43, 1997
Rat Rat, SD, Fisher Hippocampal neurons Papain: 0.2%
HibernateA/B27 Brewer, G.J.: Isolation and culture of adult rat hippocampal neurons of any age, J Neurosci Methods 71, 143, 1997
Rat Rat, E18 Hippocampal neurons Papain: 20 u/ml
Deoxyribonuclease I: 0.01%
MEM Liu QY, Schaffner AE, Li YX, Dunlap V, Barker JL: Upregulation of GABAA current by astrocytes in cultured embryonic rat hippocampal neurons, J Neurosci 16, 2912-23, 1996
Rat Rat, 1-6 day DRG neurons Papain: 20 u/ml
Collagenase Type 1: 150 u/ml
Neutral Protease: 0.8%
HBSS Robertson, S., Rae, M., Rowan, E. and Kennedy, C.: Characterization of a P2X-Purinoceptor in Cultured Neurones of the Rat Dorsal Root Ganglia., Br J Pharmacol 118, 951, 1996
Rat Rat, pups, 14 day old Neurons, sympathetic Neutral Protease: 0.24%
HBSS McFarlane, S., and Cooper, E.: Extrinsic Factors Influence the Expression of Voltage-Gated K Currents on Neonatal Rat Sympathetic Neurons, J Neurosci 13 (6), 2591, 1993
Rat Rats, SD, 11-14 day old, 29-32 g Basal forebrain neurons Trypsin: 0.125%
Gey's BSS Allen, T., Sim, J., and Brown, D.: The Whole-Cell Calcium Current in Acutely Dissociated Magnocellular Cholinergic Basal Forebrain Neurones of the Rat, J Physiol 460, 91, 1993
Rat Rat, Wistar-Hanover, 7-12 day Postnatal dopamine neurons Trypsin: 0.035%
(see reference) Rayport, S., Sulzer, D., Shi, W., Sawasdikosol, S., Monaco, J., Batson, D., and Rajendran, G.: Identified Postnatal Mesolimbic Dopamine Neurons in Culture: Morphology and Electrophysiology, J Neurosci 12 (11), 4264, 1992
Rat Rat, SD, 18-day-old, fetus Hippocampal Trypsin: 0.2%
HBSS Cheng, B., and Mattson, M.: IGF-I and IGF-II Protect Cultured Hippocampal and Septal Neurons Against Calcium-Mediated Hypoglycemic Damage, J Neurosci 12 (4), 1558, 1992
Rat Rat Stem, neural crest Collagenase: 0.075%
Ringer's solution Stemple, D., and Anderson, D.: Isolation of a Stem Cell for Neurons and Glia from the Mammalian Neural Crest, Cell 71, 973, 1992
Rat Rat, SD, pregnant, Charles River Pyramidal neurons
Nonpyramidal neurons
Trypsin: 0.027%
HEPES Buchhalter, J., and Dichter, M.: Electrophysiological Comparison of Pyramidal and Stellate Nonpyramidal Neurons in Dissociated Cell Culture of Rat Hippocampus, Brain Res Bull 26, 333, 1991
Rat Rat, SD, embryos, 19-21 days gestation Schwann, dorsal root ganglia Trypsin: 0.25%
HBSS, CMF Mithen, F., Reiker, M., and Birchem, R.: Effects of Ethanol on Rat Schwann Cell Proliferation and Myelination in Culture, In Vitro Cell Dev Biol 26, 129, 1990
Rat Rat
(also bovine)
Adrenal chromaffin
Trypsin: 0.06%
25mM HEPES buffered Locke's solution, CMF Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990
Rat Rat, SD, 8 day old pups Cerebellar neurons Trypsin: 0.025%
Eagle's MEM Novelli, A., Reilly, J., Lysko, P., and Henneberry, R.: Glutamate Becomes Neurotoxic Via the N-methyl-D-aspartate Receptor When Intracellular Energy Levels are Reduced, Brain Res 451, 205, 1988
Rat Rat, fetuses, 18 day old Hippocampal neurons Trypsin: 0.2%
Eagle's MEM Mattson, M., and Kater, S.: Isolated Hippocampal Neurons in Cryopreserved Long-Term Cultures: Development of Neuroarchitecture and Sensitivity to Nmda, Int J Dev Neurosci 6 (5), 439, 1988
Rat Rat, postnatal Septal neurons Papain: 0.05%
PBS, CMF Hatanaka,H., Tsukui, H., Nihonmatsu, I.: Septal Cholinergic Neurons From Postnatal Rat Can Survive In The Dissociate Culture Conditions In The Presence Of Nerve Growth Factor, Neurosci Lett 79, 85, 1987
Rat Rat, Long Evans, 1-15 days Neurons, visual cortex Papain: 20 u/ml
(see reference)
Huettner, J., and Baughman, R.: Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats, J Neurosci 6, 3044, 1986
Rat Rat, SD, female, 4 month old CNS cells Trypsin: 0.25%
EBSS Wood, P., and Bunge, R.: Evidence That Axons are Mitogenic for Oligodendrocytes Isolated From Adult Animals, Nature 320, 756, 1986
Rat Rat, embryo, 15 day Dorsal horn neurons
Trypsin: 0.025%
Ham's F-12 Jahr, C. and Jessell, T.: Synaptic Transmission between Dorsal Root Ganglion and Dorsal Horn Neurons in Culture: Antagonism of Monosynaptic Excitatory Postsynaptic Potentials and Glutamate Excitation by Kynurenate, J Neurosci 5, 2281, 1985
Rat Rat, fetus, 18-20 day Hippocampal neurons Trypsin: 0.25%
HBSS, CMF Bartlett, W. and Banker, G.: An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts, J Neurosci 4, 1944, 1984
Rat Rat, postnatal Ganglion, retina Papain: 12.5 u/ml
HBSS w/5 mM HEPES Leifer, D., Lipton, S., Barnstable, C., and Masland, R.: Monoclonal Antibody to Thy-1 Enhances Regeneration of Processes by Rat Retinal Ganglion Cells in Culture, Science 224, 303, 1984
Rat Rat, SD, pups Retina Trypsin: 0.25%
Ham's F-12 Sarthy PV, Curtis BM, and Catterall WA.: Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat, J Neurosci 3 (12), 2532, 1983
Rat Rat, W/FU, 5-8 day Neurons and glial Trypsin: 0.25%
(see reference)
Raff, M., Fields, K., Hakomori, S., Mirsky, R., Pruss, R., and Winter, J.: Cell-Type-Specific Markers for Distinguishing and Studying Neurons And the Major Classes of Glial Cells in Culture, Brain Res 174, 283, 1979
Rat Rat, fetus Neurons, sympathetic Trypsin: 0.25%
L-15 or HBSS, CMF Wakshull, E., Johnson, M., Burton, H.: Postnatal Rat Sympathetic Neurons In Culture. 1. A Comparison With Embryonic Neurons, J Neurophysiol 42, 1410, 1979
Rat Rat, Wistar/Furth, newborn
(also bovine)
Schwann Trypsin: 0.25%
DMEM Brockes, J., Fields, K., and Raff, M.: Studies on Cultured Rat Schwann Cells. I. Establishment of Purified Populations From Cultures of Peripheral Nerve, Brain Res 165, 105, 1979
Rat Rat, embryo Neurons, cortical Trypsin: 0.027%
MEM Dichter, M.: Rat Cortical Neurons in Cell Culture: Culture Methods, Cell Morphology, Electrophysiology, and Synapse Formation, Brain Res 149, 279, 1978
Rat Rat, newborn Neurons, sympathetic Collagenase Type 1: 0.01%
Hank's solution, CF Reichardt, L., Patterson, P.: Neurotransmitter Synthesis and Uptake by Isolated Sympathetic Neurones in Microcultures, Nature 270, 147, 1977
Rat Rat, neonatal Neurons, superior cervical ganglia Trypsin: 0.1%
Basal L-15 medium Mains, R., and Patterson, P.: Primary Cultures of Dissociated Sympathetic Neurons I. Establishment of Long-Term Growth in Culture and Studies of Differentiated Properties, J Cell Biol 59, 329, 1973
Salamander Salamander, 18-25 cm Retina Papain: 14 u/ml
Saline Townes-Anderson, E., MacLeish, P., and Raviola, E.: Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase, J Cell Biol 100, 175, 1985
Salamander Salamander (A.tigrinum) Photoreceptors, retina Papain: 0.05%
(see reference) Bader, C., MacLeish, P., and Schwartz, E.: Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina, Proc Natl Acad Sci U S A 75, 3507, 1978
Shellfish Aplysia californica Neurons Neutral Protease: 1.0%
L-15-ASW Lee, A., Decourt, B. and Suter, D.: Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones., J Vis Exp 12, 662, 2008
Shellfish Snails (Helisoma trivolis), albino, adult Buccal ganglia; SLT muscle Trypsin: 0.2%
DMEM Zoran, M., Doyle, R. and Haydon, P.: Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis, Neuron 6, 145, 1991
Shellfish Pond snail (Helisoma) albino, adult Somata, buccal ganglia Trypsin: 0.2%
Antibiotic saline, Leibowitz 50% Haydon, P.: The Formation of Chemical Synapses Between Cell-Cultured Neuronal Somata, J Neurosci 8, 1032, 1988
Shellfish Helisoma trivovlis Buccal ganglia Trypsin: 0.2%
L-15 medium Cohan, C., Haydon, P., and Kater, S.: Single Channel Activity Differs in Growing and Nongrowing Growth Cones of Isolated Identified Neurons of Helisoma, J Neurosci Res 13, 285, 1985
Shellfish Mollusc, juvenile 1-2 gm or adult 50-100 gm Neurons
LUQ cells
Protease: 1%
L15 medium Schacher, S., and Proshanksy, E.: Neurite Regeneration by Aplysia Neurons in Dissociated Cell Culture: Modulation by Aplysia Hemolymph and the Presence of the Initial Axonal Segment, J Neurosci 3 (12), 2403, 1983
Shellfish Aplysia californica LUQ cells
RUQ cells
Protease: 1%
L15 medium Camardo, J., Proshansky, E., and Schacher, S.: Identified Aplysia Neurons Form Specific Chemical Synapses in Culture, J Neurosci 3 (12), 2614, 1983
Turtle Turtle (Pseudemys scripta elegans) Retinal Papain: 0.1% (13.5 u/mg)
Kreb's Ringer Lam, D.: Biosynthesis of Acetylcholine in Turtle Photoreceptors, Proc Natl Acad Sci U S A 69, 1987, 1972