Pancreas - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Duct epithelial Collagenase: 0.1%
 HEPES Cotton, C., and Al-Nakkash, L.: Isolation and Culture of Bovine Pancreatic Duct Epithelial cells, Am J Physiol 272, G1328, 1997
Bovine Bovine (Bos taurus) Ductal Neutral Protease: 0.05%
 EBSS Sato, T., Sato, M., Hudson, E., and Jones, R.: Characterization of Bovine Pancreatic Ductal Cells Isolated by a Perfusion-Digestion Technique, In Vitro 19, 651, 1983
Bovine Bovine Platelets Trypsin:
(see reference) Stiles, G., and Lefkowitz, R.: Hormone-Sensitive Adneylate Cyclase, J Biol Chem 257 (11), 6287, 1982
Canine Canine Buccal ganglia; SLT muscle Trypsin: 0.2%
 DMEM Zoran, M., Doyle, R. and Haydon, P.: Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis, Neuron 6, 145, 1991
Fish Fish, Osphronemus gourami, 3-month-old, 0.5 mg Islets Collagenase: 0.12 - 0.46 u/ml
 RPMI 1640 Schrezenmeir, J., Laue, C., Sternheim,E., Wolbert, K., Darquy, S., Chicheportiche, D., Kirchgessner, J., and Reach, G.: Long-Term Function of Single-Cell Preparations of Piscine Principal Islets in Hollow Fibers, Transplant Proc 24 (6), 2941, 1992
Guinea-Pig Guinea-pig Acinar Collagenase Type 3: 60 u/ml
 Kreb's Ringer Schultz, G., Sarras, Jr, M., Gunther, G., Hull, B., Alicea, H., Gorelick, F., and Jamieson, J.: Guinea Pig Pancreatic Acini Prepared with Purified collagenase, Exp Cell Res 130, 49, 1980
Guinea-Pig Guinea-pig, Hartley, albino, male, 350-400 g Acinar Soybean Trypsin Inhibitor: 0.01%
 Kreb's Ringer Gardner, J., Conlon, T., Klaeveman, H., Adams, T., and Ondetti, M.: Action of Cholecystokinin and Cholinergic Agents on Calcium Transport in Isolated Pancreatic Acinar Cells, J Clin Invest 56, 366, 1975
Guinea-Pig Guinea-pig Exocrine Hyaluronidase: 0.15% - 0.2%
 Kreb's Ringer Amsterdam, J., and Jamieson, J.: Structural and Functional Characterization of Isolated Pancreatic Exocrine Cells, Proc Natl Acad Sci U S A 69 (10), 3028, 1972
Hamster Hamster Islets Collagenase Type 4: 1.3% - 2.0%
 HBSS Feldman, J., and Chapman, B.: Preparation of Islets of Langerhans from Rabbits and Hamsters by the Collagenase Digestion Technique, Acta Diabetol 12, 208, 1975
Human Human, 25-55 yrs old
(also porcine)		 Islets Collagenase: 0.4%
 HBSS Contractor, H., Johnson, P., Chadwick, D., Robertson, G., and London, N.: The Effect of UW Solution and Its Components on the Collagenase Digestion of Human and Porcine Pancreas, Cell Transplant 4 (6), 615, 1995
Human Human Islets Collagenase Type 4: 0.8%
 HBSS Izumi, R., Konishi, K., Ueno, K., Shimizu, K., Hirosawa, H., Takahashi, N., and Miyazaki, I.: Isolation of Human Pancreatic Islets from Cryopreserved Pancreas, Transplant Proc XVII, 383, 1985
Human Human Islets Collagenase (1 or 4): 0.60%
 HBSS Gray, D., McShane, P., Grant, A., and Morris, P.: A Method for Isolation of Islets of Langerhans from the Human Pancreas, Diabetes 33, 1055, 1984
Human Human, infant, age 1 day-1 year Islets Collagenase: 170-210 u/ml
 HBSS Sutherland, D., Matas, A., Steffes, M., and Najarian, J.: Infant Human Pancreas: A Potential Source of Islet Tissue for Transplantation, Diabetes 25 (12), 1123, 1976
Monkey Monkey, 3-5 Kg Islets Hyaluronidase: 0.05%
 HBSS Scharp, D., Murphy, J., Newton, W., Ballinger, W., and Lacy, P.: Application of an Improved Isolation Technique for Islet Transplantation in Primates and Rats, Transplant Proc 7, 739, 1975
Mouse Mouse Pancreatic islet Collagenase Type 4: 0.2%
 HBSS Haefliger Jacques-Antoine, Tawadros Thomas, Meylan Laure, Gurun SabineLe, Roehrich Marc-Estienne, Martin David, Thorens Bernard, Waeber Gerard: The scaffold protein IB1/JIP-1 is a critical mediator of cytokine-induced apoptosis in pancreatic beta cells, J Cell Sci 116, 1463-9, 2003
Mouse Mouse Islets Collagenase Type 4: 0.2%
 HBSS Wu Yulian, Han Bing, Luo Hongyu, Roduit Raphael, Salcedo TheodoraW, Moore PaulA, Zhang Jun, Wu Jiangping: DcR3/TR6 effectively prevents islet primary nonfunction after transplantation, Diabetes 52, 2279-86, 2003
Mouse Mouse, BALB/c, 6-8 week-old, either sex Acinar Collagenase: 0.1%
 Waymouth's MB Kurup, S., and Bhonde, R.: Analysis and Optimization of Nutritional Set-up for Murine Pancreatic Acinar Cells, JOP 3 (1), 8, 2002
Mouse Mouse Islets Collagenase Type 2: 0.2%
 CF Medium Koster, J., Marshall, B., Ensor, N., Corbett, J., and Nichols, C.: Targeted Overativity of Cell KATP Channels Induces Profound Neonatal Diabetes, Cell 100, 645, 2000
Mouse Mouse Pancreatic islets Collagenase Type 4: 0.4%
 Gey's BSS Strowski M, Parmar R, Blake A, Schaeffer J: Somatostatin inhibits insulin and glucagon secretion via two receptors subtypes: an in vitro study of pancreatic islets from somatostatin receptor 2 knockout mice, Endocrinology 141, 111-7, 2000
Mouse Mouse Duct Papain: 25 u/ml
 DMEM /F-12 Githens, S: Pancreatic Duct Epithelial Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:12.1, 1995
Mouse Mouse Acinar Collagenase: 100 u/ml
 HEPES Jauch, P., Peterson, O., and Lauger, P.: Electrogenic Properties of the Na-Alanine Cotransporter in Acinar Cells, J Membr Biol 94, 99, 1986
Mouse Mouse (C57BL/6J-ob/ob), 9-12 wks, male Islets Hyaluronidase: 0.5%
 Kreb's Ringer bicarbonate buffer Dalpe-Scott, M., Heick, H., and Begin-Heick, N.: Secretion in the Obese (ob/ob) Mouse. The Effect of Oxytetracycline on Insulin Release, Diabetes 32, 932, 1983
Mouse Mouse, male, 18-24 g Acinar CLSPA: 70-90 u/ml
Soybean Trypsin Inhibitor: 0.01%
 Krebs-Henseleit Burnham DB, Williams JA: Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini, J Biol Chem 257, 10523-8, 1982
Porcine Porcine, juvenile Islets Collagenase: see reference
 Univ of Wisconsin solution Van der Burg Michael P M, Graham John M: Iodixanol Density Gradient Preparation in University of Wisconsin solution for porcine islet purification, ScientificWorldJournal 3, 1154-9, 2003
Porcine Porcine, 3 month, 15-20 kg Acinar Collagenase Type 3: 200 u/ml
 RPMI 1640 Zhao, X., Han, J., and Tang, C.: Primary Culture of Porcine Pancreatic Acinar Cells, JOP 2 (2), 78, 2001
Porcine Porcine, 1-3 year, 1.5-2.0 kg, either sex Islets Collagenase: 0.25%
 HBSS Korbutt, G., Elliott, J., Ao, Z., Smith, D., Warnock, G., and Rajotte, R.: Large Scale Isolation, Growth, and Function of Porcine Neonatal Islet Cells, J Clin Invest 97 (9), 2119, 1996
Porcine Porcine, female Islets Collagenase: 0.1%
 HBSS Brandhorst, D., Brandhorst, H., Hering, B., Federlin, K., and Bretzel, R.: Islet Isolation from the Pancreas of Large Mammals and Humans: 10 Years of Experience, Exp Clin Endocrinol 103, 3, 1995
Porcine Porcine Islets Collagenase: 0.1%-0.2%
 HBSS Johnson, P., van Suylichem, P., Roberts, D., Vos-Scheperkeuter, G., White, S., van Schlifgaarde, R., London, N.: Design of a Simple, in vitro Method for Evaluation of the Efficiency of Crude Clostridium histolyticum Collagenase and its Components for Porcine Islet Isolation, Xenotransplantation 2, 165, 1995
Porcine Porcine, <2 year, 200-250 kg Islets Collagenase: 0.1%
 HBSS Heiser, A., Ulrichs, K., and Muller-Ruchholtz, W.: Isolation of Porcine Pancreatic Islets: Low Trypsin Activity During the Isolation Procedure Guarantees Reproducible High Islet Yields, J Clin Lab Anal 8, 407, 1994
Porcine Porcine, 10-36 months, 200-300 kg Islets Collagenase: 0.2%
 HBSS Ricordi, C., Socci, C., Davalli, A., Staudacher, C., Baro, P., Vertova, A., Sassi, I., Gavazzi, F., Pozza, G., and Di Carlo, V.: Isolation of the Elusive Pig Islet, Surgery 107 (6), 688, 1990
Porcine Porcine Acinar Collagenase: 100 u/ml
 Saline Iwatsuki, N., and Peterson, O.: Action of Tetraethylammonium on Calcium-Activated Potassim Channels in Pig Pancreatic Acinar Cells Studied by Patch-Clamp Single-Channel and Whole-Cell Current Recording, J Membr Biol 86, 139, 1985
Rabbit Rabbit, New Zealand white, Male/Female, 2-3 Kg Acinar Hyaluronidase: 0.2%
 Kreb's Ringer bicarbonate buffer Renckens, B., Schrijen, J., Swarts, H., DePont, J., and Bonting, S.: Role of Calcium in Exocrine Pancreatic Secretion. IV. Calcium Movements in Isolated Acinar Cells of Rabbit Pancreas, Biochim Biophys Acta 544, 338, 1978
Rabbit Rabbit, also hamster Islets Collagenase Type 4: 1.3% - 2.0%
 HBSS Feldman, J., and Chapman, B.: Preparation of Islets of Langerhans from Rabbits and Hamsters by the Collagenase Digestion Technique, Acta Diabetol 12, 208, 1975
Rat Rat, Wistar, male, 250-400g Pancreatic islets Collagenase: 0.75%
 RPMI 1640 Tian XH, Xue WJ, Ding XM, Pang XL, Teng Y, Tian PX, and Feng XS: Small intestinal submucosa improves islet survival and function during in vitro culture, World J Gastroenterol 11, 7378, 2005
Rat Rat, 250-350 g Pancreatic islet Collagenase Type 4: 0.2%
 HBSS Haefliger Jacques-Antoine, Tawadros Thomas, Meylan Laure, Gurun SabineLe, Roehrich Marc-Estienne, Martin David, Thorens Bernard, Waeber Gerard: The scaffold protein IB1/JIP-1 is a critical mediator of cytokine-induced apoptosis in pancreatic beta cells, J Cell Sci 116, 1463-9, 2003
Rat Rat Pancreatic acini CLSPA: 30 u/ml
Collagenase Type 4: 30 u/ml
Soybean Trypsin Inhibitor: 0.01%
 M199 Blinman TA, Gukovsky I, Mouria M, Zaninovic V, Livingston E, Pandol SJ, Gukovskaya AS: Activation of pancreatic acinar cells on isolation from tissue: cytokine upregulation via p38 MAP kinase, Am J Physiol Cell Physiol 279, C1993-2003, 2000
Rat Rat, SD, male Ancinar CLSPA: see reference
Soybean Trypsin Inhibitor: 0.01%
 DMEM Ji B, Kopin AS, Logsdon CD: Species differences between rat and mouse CCKA receptors determine the divergent acinar cell response to the cholecystokinin analog JMV-180, J Biol Chem 275, 19115-20, 2000
Rat Rat Islets Collagenase: 126 - 196 u/ml
 HBSS Verspohl, E., and Wienecke, A.: The Role of Protein Kinase C in the Desensitization of Rat Pancreatic Islets to Chrolinergic stimulation, J Endocrinol 159, 287, 1998
Rat Rat Islets Collagenase: 0.5 - 0.9%
 HBSS Takaki, R and Ono J: Culture of Pancreatic Islet Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:11.1, 1995
Rat Rat Duct Papain: 25 u/ml
 DMEM /F-12 Githens, S: Pancreatic Duct Epithelial Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:12.1, 1995
Rat Rat, SD, male, 40 - 100 g Acinar Hyaluronidase: 462 u/ml
 Ham's F12 Hirschi, K., Kenny, S., Justice, J., Brannon, P.: Effects of Secretin And Caerulein On Enzymes Of Cultured Pancreatic Acinar Cells, In Vitro Cell Dev Biol 27, 660, 1991
Rat Rat, SD, male, 100 - 150 g Parotid acinar Trypsin: 0.001%
 F12 medium Yeh, C., Mertz, P., Oliver, C., Baum, B., and Kousvelari, E.: Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells, In Vitro Cell Dev Biol 27, 707, 1991
Rat Rat, Wistar, male Parotid acinar Trypsin: 0.02%
 Solution B
(See reference)		 Foskett, J., Roifman, C. and Wong, D.: Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells, J Biol Chem 266, 2778, 1991
Rat Rat, S-Wistar, male, 230 - 270 g
(also mouse, 6 - 8 wk old)		 Islets Collagenase: 0.1% - 0.2%
 HBSS Ohzato, H., Gotoh, M., Monden, M., Dono, K., Kanai, T., and Mori, T.: Improvement in the Islet Yield From a Cold-Preserved Pancreas by Pancreatic Ductal Collagenase Distention at the Time of Harvesting, Transplantation 51, 566, 1991
Rat Rat, SD, both sexes, 6-20 wks old Interlobular ducts Papain: 25 u/ml
 DMEM/Ham's F-12 Githens, S., Schexnayder, J., Desai, K., and Patke, C.: Rat Pancreatic Interlobular Duct Epithelium: Isolation and Culture in Collagen Gel, In Vitro Cell Dev Biol 25 (8), 679, 1989
Rat Rat, SD, male, 150 - 200 g Acinar Soybean Trypsin Inhibitor: 0.01%
 HEPES Menozzi, D., Sato, S., Jensen, R., and Gardner, J.: Cyclic GMP Does Not Inhibit Protein Kinase C-Mediated Enzyme Secretion in Rat Pancreatic Acini, J Biol Chem 264, 995, 1989
Rat Rat, Wistar, male, 250 - 350 g Acinar, parotid Hyaluronidase: 0.015%
 Earle's MEM Melvin, J., Kawaguchi, M., Baum, B., and Turner, R.: A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells, Biochem Biophys Res Commun 145, 754, 1987
Rat Rat, SD, male, 50 - 125 g Acinar
Exorbital lacrimal, parotid, pancreas		 Trypsin: 0.01%
 HBSS, CMF Oliver, C., Waters, J., Tolbert, C., and Kleinman, H.: Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel, In Vitro Cell Dev Biol 23, 465, 1987
Rat Rat, Fischer-344, either sex, 120-150 g Epithelial Trypsin: 0.1%
 Ham's F-12/HBSS
(see reference)		 Tsao, M., and Duguid, W.: Establishment of Propagable Epithelial Cell Lines From Normal Adult Rat Pancreas, Exp Cell Res 168, 365, 1987
Rat Rat, SD, male, 42 - 48 day, 175 - 200 g Acinar, submandibular gland Hyaluronidase: 0.1 %
 HBSS, CF Quissell, D., Redman, R., and Mark, M.: Short-Term Primary Culture of Acinar-Intercalated Duct Complexes From Rat Submandibular Glands, In Vitro Cell Dev Biol 22, 469, 1986
Rat Rat, Dark Agouti and Lewis rat donors, adult Islets Collagenase Type 1: 0.3%
 Hank's solution Sutton, R., Peters, M., McShane, P., Gray, D., and Morris, P.: An Improved Method for the Isolation of Islets of Langerhans From the Adult Rat Pancreas, Transplant Proc XVII (6), 1819, 1986
Rat Rat, SD, male, 50-75 g Acinar Hyaluronidase: 0.1%
 HBSS
(see reference)		 Brannon, P., Orrison, B., and Kretchmer, N.: Primary Cultures of Rat Pancreatic Acinar Cells in Serum-Free Medium, In Vitro Cell Dev Biol 21 (1), 6, 1985
Rat Rat, SD, 125 - 350 g Duct Trypsin: 0.01%
 HBSS Githens, S., Holmquist, D., Whelan, J., and Ruby, J.: Ducts of the Rat Pancreas in Agarose Matrix Culture, In Vitro 16, 797, 1980
Rat Rat Exocrine Hyaluronidase: 0.9%
 Kreb's Ringer Schulz, I., Heil, K., Kribben, A., Sachs, G., and Haase, W.: Isolation and Functional Characterization of Cells From the Exocrine Pancreas,Biology of Normal and Cancerous Exocrine Pancreatic Cells,Ribet, A., Pradayrol, L., and Susini, C., Elsevier, , 1980
Rat Rat, Wistar, male Islets Collagenase Type 4: 1%
 Medium 199 Katada, T., and Ui, M.: Enhanced Insulin Secretion and Cyclic Amp Accumulation in Pancreatic Islets Due to Activation of Native Calcium Ionophores, J Biol Chem 254 (2), 469, 1979
Rat Rat, SD, male, 250 - 350 g
(also mouse, white, Swiss, male, 20 - 24 g)		 Acinar Hyaluronidase: 0.18%
 Kreb's Henseleit bicarbonate buffer Williams, J., Korc, M., and Dormer, R.: Action of Secretagogues on a New Preparation of Functionally Intact, Isolated Pancreatic Acini, Am J Physiol 235, 517, 1978
Rat Rat, Wistar, male, 200 - 300 g Islets Collagenase Type 4: 0.5%
 HBSS Shibata, A., Ludvigsen, C., Naber, S., McKaneil M., and Lacy, P.: Standardization of a Digestion-Filtration Method for Isolation of Islets, Diabetes 25, 667, 1976
Rat Rat Exocrine Hyaluronidase: 0.15%
 Krebs Kondo, S., and Schulz, I.: Calcium Ion Uptake in Isolated Pancreas Cells Induced by Secretagogues, Biochim Biophys Acta 419, 76, 1976
Rat Rat, Wistar-Lewis, fetal 18-20 day gestation
(also neonatal 3-12 day old)		 Islets Collagenase Type 4: 0.63%
 EBSS
(see reference)		 Braaten, J., Jarlfors, U., Smith, D., and Mintz, D.: Purification of Monolayer Cell Cultures of the Endocrine Pancreas, Tissue Cell 7 (4), 747, 1975
Rat Rat, neonate Islets Trypsin: 0.05%
 Puck's saline buffered w/ EDTA 0.02% Leonard, R., Lazarow, A., and Hegre, O.: Pancreatic Islet Transplantation in the Rat, Diabetes 22, 413, 1973
Rat Rat, male, albino, 200-300 g Islets Collagenase: 0.5%
 Hanks solution Lacy, P., Walker, M., and Fink, J.: Perifusion of Isolated Rat Islets in Vitro, Diabetes 21 (10), 987, 1972
Rat Rat, Lewis, inbred Islets Collagenase: 0.5%
 HBSS Ballinger, W., and Lacy, P.: Transplantation of Intact Pancreatic Islets in Rats, Surgery 72 (2), 175, 1972
Rat Rat, Wistar, albino, male, 400 - 500 g Islets Collagenase Type 4: 1.0% - 1.2%
 HBSS Lacy, P., and Kostianovsky, M.: Method for the Isolation of Intact Islets of Langerhans from the Rat Pancreas, Diabetes 16, 35, 1967
Rat Rat Ascites hepatoma Trypsin: 0.1%
 Phosphate buffer (see reference) Essner, E.: Experiments on an Ascites Hepatoma. I. Enzymatic Digestion and Alkaline Degradation of the Cementing Substance and Separation of Cells, in Tumor Islands, Exp Cell Res 7, 430, 1954