Spleen - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Mouse Mouse Splenic Collagenase Type 2: 0.1%
PBS Klebanoff, C., Spencer, S., Torabi-Parizi, P., Grainger, J., Roychoudhuri, R., Ji, Y., Sukumar, M., Muranski, P., Scott, C., Hall, J., Ferreyra, G., Leonardi, A., Borman, Z. et al.: Retinoic Acid Controls the Homeostasis of Pre-cDC-Derived Splenic and Intestinal Dendritic Cells., J Exp Med 210, 1961, 2013
Mouse Mouse Splenocytes Collagenase Type 2: 0.16%
Deoxyribonuclease I: 0.002%
PBS Yi, T. and Cyster, J.: EBI2-Mediated Bridging Channel Positioning Supports Splenic Dendritic Cell Homeostasis and Particulate Antigen Capture., Elife 2, e00757, 2013
Mouse Mouse Spleen, bone marrow endothelial Collagenase Type 4: 0.3-1.0%
Deoxyribonuclease I: 20 u/ml
PBS Shi, C., Jia, T., Mendez-Ferrer, S., Hohl, T., Serbina, N., Lipuma, L., Leiner, I., Li, M., Frenette, P. and Pamer, E.: Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands., Immunity 34, 590, 2011
Mouse Mouse, female, 6-8 week Dendritic Collagenase Type 4: 0.05%
RPMI 1640 Abou Fakher, F., Rachinel, N., Klimczak, M., Louis, J. and Doyen, N.: TLR9-Dependent Activation of Dendritic Cells by DNA from Leishmania Major Favors Th1 Cell Development and the Resolution of Lesions., J Immunol 182, 1386, 2009
Mouse Mouse Dendritic Collagenase Type 1: 0.5%
HBSS Flano, E., Jewell, N., Durbin, R. and Durbin, J.: Methods Used to Study Respiratory Virus Infection.,Curr Protoc Cell Biol Vol. Chapter 26,, , Unit 26.3, 2009
Mouse Mouse Dentritic Collagenase: 300 u/ml
Deoxyribonuclease I: 0.002%
RPMI 1640 Abe, K., Nguyen, K., Fine, S., Mo, J., Shen, C., Shenouda, S., Corr, M., Jung, S., Lee, J., Eckmann, L. and Raz, E.: Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells., Proc Natl Acad Sci U S A 104, 17022, 2007
Mouse Mouse Splenic Collagenase Type 2: 0.1%
Deoxyribonuclease I: 0.1%
RPMI Mueller, S., Matloubian, M., Clemens, D, Sharpe, A., Freeman, G., Gangappa, S., Larsen, C. and Ahmed, R.: Viral Targeting of Fibroblastic Reticular Cells Contributes to Immunosuppression and Persistence During Chronic Infection., Proc Natl Acad Sci U S A 104, 15430, 2007
Mouse Mouse Splenic dendritic Collagenase: 0.1%
Deoxyribonuclease I: 0.005%
DMEM Kang, H., Liu, M. and Datta, S.: Low-dose Peptide Tolerance Therapy of Lupus Generates Plasmacytoid Dendritic Cells That Cause Expansion of Autoantigen-Specific Regulatory T Cells and Contraction of Inflammatory Th17 Cells, J Immunol 178, 7849-58, 2007
Mouse Mouse Splenic stromal Collagenase Type 3: 100-400 u/ml
HBSS Benedict Chris A, De Trez Carl, Schneider Kirsten, Ha Sukwon, Patterson Ginelle, Ware Carl F: Specific remodeling of splenic architecture by cytomegalovirus, PLoS Pathog 2, e16, 2006
Mouse Mouse Leukocytes Collagenase Type 4: 43 u/ml
RPMI 1640 Siragam, V., Crow, A., Brinc, D., Song, S., Freedman, J. and Lazarus, A.: Intravenous Immunoglobulin Ameliorates ITP via Activating Fc Gamma Receptors on Dendritic Cells., Nat Med 12, 688, 2006
Mouse Mouse, 4-6 week Dentritic Collagenase Type 1: 0.1%
Deoxyribonuclease I: 0.001%
RPMI-1640 McLellan, A., Kapp, M., Eggert, A., Linden, C., Bommhardt, U., Brocker, E., Kammerer, U. and Kampgen, E.: Anatomic Location and T-cell Stimulatory Functions of Mouse Dendritic Cell Subsets Defined by CD4 and CD8 Expression, Blood 99, 2084-93, 2002
Mouse Mouse Dentritic Collagenase Type 3: 0.1%
Deoxyribonuclease I: 325 u/ml
RPMI 1640 Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F, and Gabrieli, L: ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells, J Exp Med 196, 1415, 2002
Mouse Mouse Dentritic cells Collagenase Type 4: 0.1%
Deoxyribonuclease I: 20 ug/ml
HEPES/RPMI 1640 Stagg, AJ, Burke, F, Hill, S and Knight, S.: Isolation of Mouse Spleen Dendritic Cells., Methods Mol Med 64, 9-22, 2001
Mouse Mouse Dentritic Collagenase: 100 u/ml
HBSS Brasel K, De Smedt T, Smith JL, Maliszewski CR: Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures, Blood 96, 3029-39, 2000