Spleen - Tissue Tables

Species	Species Detail	Cells	Enzyme(s)	Medium	Reference
Mouse	Mouse	Splenic stromal	Collagenase Type 3: 100-400 u/ml	HBSS	Benedict Chris A, De Trez Carl, Schneider Kirsten, Ha Sukwon, Patterson Ginelle, Ware Carl F: Specific remodeling of splenic architecture by cytomegalovirus, PLoS Pathog 2, e16, 2006
Mouse	Mouse, 4-6 week	Dentritic	Collagenase Type 1: 0.1% Deoxyribonuclease I: 0.001%	RPMI-1640	McLellan AlexanderD, Kapp Michaela, Eggert Andreas, Linden Christian, Bommhardt Ursula, Bröcker Eva-B, Kämmerer Ulrike, Kämpgen Eckhart: Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression, Blood 99, 2084-93, 2002
Mouse	Mouse	Dentritic	Collagenase Type 3: 0.1% Deoxyribonuclease I: 325 u/ml	RPMI 1640	Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F, and Gabrieli, L: ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells, J Exp Med 196, 1415, 2002
Mouse	Mouse	Dentritic	Collagenase: 100 u/ml	HBSS	Brasel K, De Smedt T, Smith JL, Maliszewski CR: Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures, Blood 96, 3029-39, 2000