Thyroid/Parathyroid - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine, adult Parathyroid Deoxyribonuclease I: 0.005%,
 HEPES Ham's F10 Nygren, P., Gylfe, E., Larsson, R., Johansson, H., Juhlin, C., Klareskoq, L., Akerstrom, G., and Rastad, J.: Modulation of the Ca2+-Sensing Function of Parathyroid Cells In Vitro and in Hyperparathyroidism, Biochim Biophys Acta 968, 253, 1988
Bovine Bovine Parathyroid glands Deoxyribonuclease I: 0.0075%
 HEPES buffer Wallace, J., and Scarpa, A.: Regulation of Parathyroid Hormone Secretion in Vitro by Divalent Cations, J Biol Chem 257, 10613, 1982
Bovine Bovine Parathyroid Deoxyribonuclease I: 0.004%
 Eagle's #2 medium without bicarbonate Brown, E., Hurwitz, S., and Aurbach, G.: Preparation of Viable Isolated Bovine Parathyroid Cells, Endocrinology 99, 1582, 1976
Bovine Bovine
(also porcine)		 Thyroid Trypsin: 0.004%
 EBSS Tong, W.: The Isolation and Culture of Thyroid Cells, Meth Enzymol 32, 745, 1974
Chicken Chick embryo Thyroid
Muscle
Heart		 Collagenase: 0.25%
 Tyrode's saline, potassium free Hilfer, S., and Brown, J.: Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart, Exp Cell Res 65, 246, 1971
Chicken Chicken, Rhode Island Red, embryo Thyroid follicular Collagenase: 0.2%
 Tyrode's solution, CMF Spooner, B.: The Expression of Differentiation by Chick Embryo Throid in Cell Culture. I. Functional and Fine Structural Stability in Mass and Clonal Culture, J Cell Physiol 75, 33, 1970
Porcine Porcine
(also bovine)		 Thyroid Trypsin: 0.004%
 EBSS Tong, W.: The Isolation and Culture of Thyroid Cells, Meth Enzymol 32, 745, 1974
Sheep Sheep Thyroid Collagenase: 0.2%
 Puck's Saline F Kerkof, P.: Preparation of Primary Cultures of Ovine Thyroid Gland Cells, J Tiss Cul Meth 7, 23, 1982