Worthington Tissue Dissociation Guide
Dissociating Enzymes: Hyaluronidase
Hyaluronidase is a polysaccharidase with a specificity for endo-N-acetylhexosaminic bonds between 2-acetoamido-2-deoxy-beta-D-glucose and D-glucuronate. These bonds are common in hyaluronic acid and chondroitin sulfate A and C. Because these substances are found in high concentrations in the ground substance of virtually all connective tissues, hyaluronidase is often used for the dissociation of tissues, usually in combination with a crude protease such as collagenase.
More Information: Worthington Hyaluronidase
Tissue Tables (references, grouped by tissue type and species)
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.