Worthington Tissue Dissociation Guide
Optimization Techniques: Measure of Viability
One of the simplest methods to approximate cell viability is the dye exclusion technique. This method utilizes an indicator dye to demonstrate cell membrane damage. Cells which absorb the dye become stained and are considered non-viable. Dyes such as trypan blue, erythrosin, and nigrosin are commonly used with trypan blue being the most common in preliminary cell isolation procedures.
This procedure can be performed along with the cell counting procedure but cell density may require adjustment in order to obtain approximately 106 cells per milliliter.Procedure
Total Cells / 1 mm2 = 182
Note: Dye exclusion viability procedures tend to give high estimates of cell viability when compared to cell attachment or metabolic assays, but for optimization of cell isolation procedures trypan blue does provide a rapid estimate of dissociation performance in conjunction with yield quantitation.
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Tissue Tables (references, grouped by tissue type and species)
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.