Worthington Tissue Dissociation Guide
Methods and Materials: Trituration
(Cell dispersion through mild pumping action)
This can be a crucial procedure. It serves to break up the tissue fragments following incubation in the dissociation mix. If done too vigorously, cells will be destroyed lowering viability; too weakly and tissue fragments will be left intact lowering yield. Gentle trituration, using a 10 ml pipette, constitutes filling and emptying the barrel at a rate of about 3.0 ml per sec. You can best determine a suitable rate for your tissue through trial and error. Avoid bubbling the cell suspension.
Tissue Tables (references, grouped by tissue type and species)
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.