A complete method development kit containing an assortment of enzymes most frequently used in enzymatic tissue dissociation and cell isolation procedures. Includes instructions, references and strategies for the handling, use and optimization of enzymatic cell isolation methods for maximum yield of viable cells. Contains all enzymes commonly referenced in tissue dissociation and cell isolation procedures and the Cell Isolation Guide describing the tissue types commonly used, the mode of action of the various enzymes, tissue culture techniques, and protocol optimization guidelines, with cell- and tissue-specific references for getting started in enzymatic cell isolation.
Tissue dissociation and cell harvesting are two principal applications for enzymes in tissue culture research and cell biology studies. Despite the widespread use of enzymes for these applications over the years, their mechanisms of action in dissociation and harvesting are not well understood. As a result, the choice of one technique over another is often arbitrary and based more on past experience than on an understanding of why the method works and what modifications could lead to even better results.
Researchers searching the scientific literature for information on the ideal enzymes and optimal conditions for tissue dissociation are often confronted with conflicting data. Much of the variation stems from the complex and dynamic nature of the extracellular matrix and from the historical use of relatively crude, undefined enzyme preparations for cell isolation applications. The extracellular matrix is composed of a wide variety of proteins, glycoproteins, lipids and glycolipids, all of which can differ in abundance from species to species, tissue to tissue and with developmental age. The Worthington Cell Isolation Optimizing System provides an assortment of the widely used enzymes in purified form for establishing an optimized cell isolation procedure on a cost-efficient basis.
• Collagenase Type 1 CLS1 500mg dw • Hyaluronidase HSE 50,000un