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Worthington Biochemical Proteinase K

Source: Tritirachium album limber


Proteinase K (PROK) is a serine protease with broad specificity towards aliphatic, aromatic and other hydrophobic amino acids. PROK has a molecular weight of 27,000 daltons and is Ca2+ dependent. It is not inactivated by metal ion chelating agents such as EDTA, sulfhydryl reagents, PCMB, TLCK, or TPCK. It also retains activity in 0.5% SDS. It can be inhibited by PMSF or DFP.

Stability/Storage: The lyophilized powder is stable for one year at 2-8°C. Solutions in 50mM Tris-HCl, pH 8.0 with 1mM CaCl2 are stable for months at 2-8°C. Store at 2-8°C.

Unit Definition: One Unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C, pH 7.5, using urea denatured hemoglobin as the substrate.

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Description Activity Code Size Cat No. Price
Proteinase K
A lyophilized powder. Purified to remove DNase and RNase.
Store at 2-8°C.
≥20 units per mg dry weight PROK 25 mg LS004220 $37.00
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100 mg LS004222 $78.00
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1 gm LS004224 $595.00
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Bulk (mg) LS004226 Inquire
Proteinase K, Solution
A concentrated, ready to use liquid formulation. Proteinase K prepared at 20mg/ml in 10mM Tris-HCl, 1mM calcium acetate, pH 7.5 containing 50% glycerol. DNase- and RNase- free.
Store at -20°C.
≥400 u/ml PROKS 5 ml LS004240 $112.00
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25 ml LS004242 $450.00
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Bulk (ml) LS004244 Inquire

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