Source: Tritirachium album limber
Proteinase K (PROK) is a serine protease with broad specificity towards aliphatic, aromatic and other hydrophobic amino acids. PROK has a molecular weight of 27,000 daltons and is Ca2+ dependent. It is not inactivated by metal ion chelating agents such as EDTA, sulfhydryl reagents, PCMB, TLCK, or TPCK. It also retains activity in 0.5% SDS. It can be inhibited by PMSF or DFP.
Stability/Storage: The lyophilized powder is stable for one year at 2-8°C. Solutions in 50mM Tris-HCl, pH 8.0 with 1mM CaCl2 are stable for months at 2-8°C. Store at 2-8°C.
Unit Definition: One Unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C, pH 7.5, using urea denatured hemoglobin as the substrate.
Albumin, Nuclease-Free (BSANF)
Deoxyribonuclease I (DP/D/DCLS/D2/DPFF/DPRF)
E•RASE RNase A/T1 Blend (RCT)
Histones (H, NHL)
Nuclease, Micrococcal (NFCP)
Nuclease, S1 (SINUC/SINUCL)
Nucleic Acids, DNA, E.coli, Lambda/Fragments,RNA
Phosphatase, Alkaline (CAP/BAPF/BAPC/BAPSF/PC)
Phosphodiesterase I (VPH)
Phosphodiesterase II (SPH)
Reverse Transcriptase, Recombinant HIV (RTHIV)
Ribonuclease A (R/RAF/RASE/RS/RPDF)
Ribonuclease T1, Animal Origin Free (RT1S)
A lyophilized powder. Purified to remove DNase and RNase.
Store at 2-8°C.
|≥20 units per mg dry weight
||Proteinase K, Solution
A concentrated, ready to use liquid formulation. Proteinase K prepared at 20mg/ml in 10mM Tris-HCl, 1mM calcium acetate, pH 7.5 containing 50% glycerol. DNase- and RNase- free.
Store at -20°C.