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Worthington Biochemical Deoxyribonuclease I

Source: Bovine Pancreas

I.U.B.: 3.1.21.1

Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.

Uses: Worthington offers DNase at different levels of purity for different applications. Product Codes: DPRF and DPRFS are both especially designed for Molecular Biology applications and contain the lowest levels of RNase and protease activity. They are both suitable for use in techniques requiring digestion of DNA in the recovery of intact RNA or where the integrity of structural proteins or enzymes must be maintained. Applications have included nick translation, DNA mapping, isolation of nuclear RNA and protein, plasmid construction, and RNA polymerase synthesis of RNA probes and RT-PCR. DNase is also used in tissue culture work to digest DNA from damaged cells thereby reducing viscosity, and removing membrane bound DNA fragments. Worthington Codes: DP and DCLS are suitable for these applications.

Stability/Storage: When properly stored, all grades of Worthington deoxyribonuclease are stable for 2-3 years. Recommended storage temperature for all grades of Worthington DNases is 2-8°C. Product code DPRFS may be stored at -20°C. For long term storage in solution, Product Codes D and DPFF may be dissolved in 5mM acetate, 1mM calcium, pH 4.5 and stored in single use aliquots at -20°C or -70°C for up to one year. Only freeze and thaw once; thawed aliquots are stable refrigerated at least several weeks. Addition of 50% glycerol will maintain a liquid state at -20°C without affecting stability and material in 50% glycerol can be removed and returned to -20°C repeatedly. DPRF is unusually stable due to the absence of protease. For long term storage of DPRF after reconstitution, use water or any buffer pH 4.0 to 9.0 except phosphate; avoid calcium chelators; add 50% glycerol for storage as liquid at -20°C; aliquot in single use containers; only freeze and thaw once; thawed aliquots are stable refrigerated at least several weeks.

Unit Definition: 1 Unit causes an increase in absorbance at 260nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0. Note: Kunitz units as reported by other suppliers can be 2 to 4 times higher than Kunitz units as measured at Worthington. As measured at Worthington, 0.005 Kunitz unit digests 1µg of lambda DNA in 10 minutes at 37°C in 50mM Tris, 1mM Mg2+, pH 7.8 in a 50µl reaction. Correlation of digestion units with Kunitz units is different for other DNA and buffer systems.

Technical Note: Product Code DPRF: Each vial contains approximately 2mg glycine and 2µmoles calcium per 10,000 units of DNase I. Dissolving the entire vial in 5ml provides the equivalent of a 1 mg/ml solution.

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Description Activity Code Size Cat No. Price
Deoxyribonuclease I, RNase & Protease Free, Solution
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Supplied as a solution at approximately 2 Kunitz units per microliter (approximately 1 mg/ml) containing 50% glycerol and 1mM calcium chloride.
Store at -20°C.
≥2,000 Kunitz units per ml DPRFS 100 un LS006342 $28.00
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500 un LS006344 $75.00
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Bulk (un) LS006348 Inquire
Deoxyribonuclease I, Ribonuclease&Protease Free
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Lyophilized in vial containing glycine and calcium as stabilizer.
Store at 2-8°C. PROTECT FROM MOISTURE.
≥2,000 Kunitz units per mg dry weight DPRF 2500 un LS006331 $43.00
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10 ku LS006333 $154.00
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50 ku LS006343 $560.00
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Bulk (un) LS006334 Inquire
Deoxyribonuclease I, Recombinant, Solution
Animal Free/AF. Recombinant Bovine pancreatic deoxyribonuclease 1 produced in Pichia pastoris. Chromatographically purified. Free of animal derived components, RNases & proteases. A liquid preparation in 5mM Calcium Acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol. Supplied with 10x reaction buffer.
Store at -20°C.
≥2 units per microliter DR1S 2 ku LS006353 $49.00
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5x2 ku LS006355 $196.00
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Bulk (ku) LS006357 Inquire
Deoxyribonuclease I
Chromatographically purified. A lyophilized powder containing glycine as a stabilizer. Contains ≤0.0005% RNase.
Store at 2-8°C. PROTECT FROM MOISTURE.
≥2,000 Kunitz units per mg dry weight DPFF 25 ku LS006330 $89.00
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125 ku LS006328 $328.00
Add to Cart
Bulk (ku) LS006332 Inquire
Deoxyribonuclease I
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.
Store at 2-8°C. PROTECT FROM MOISTURE.
≥2,000 Kunitz units per mg dry weight D 5 mg LS002004 $34.00
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20 mg LS002006 $80.00
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100 mg LS002007 $285.00
Add to Cart
Bulk (mg) LS002009 Inquire
Deoxyribonuclease I, Filtered
Filtered through a 0.22 micron membrane and lyophilized in vials. Material is not tested for pyrogenicity.
Store at 2-8°C. PROTECT FROM MOISTURE.
≥2,000 Kunitz units per mg dry weight DCLS 11 mg LS002058 $96.00
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25 mg LS002060 $170.00
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Deoxyribonuclease I, Standard Vial
Lyophilized in vials for assay standardization. Labeled to show established activity. Not suitable for assays at neutral pH.
Store at 2-8°C.
~2,000 Kunitz units per vial DSV 2 ku LS002173 $20.00
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5x2 ku LS002172 $58.00
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DNase Vial (D2)
A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of EBSS or equivalent yields a solution of 2000 units/ml of deoxyribonuclease (1 mg/ml).
Store at 2-8°C.
≥1,000 Kunitz units per vial D2 1 vi LK003170 $24.00
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5 vi LK003172 $82.00
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Deoxyribonuclease I
Partially purified. A lyophilized powder.
Store at 2-8°C.
≥2,000 Kunitz units per mg dry weight DP 25 mg LS002138 $39.00
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100 mg LS002139 $94.00
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1 gm LS002140 $780.00
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Bulk (mg) LS002141 Inquire
Deoxyribonuclease I
Partially purified. Supplied as lyophilized powder.
Store at 2-8°C.
≥1,250 Kunitz units per mg dry weight DPB 100 mg LS002145 $80.00
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1 gm LS002147 $570.00
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Bulk (mg) LS002149 Inquire
Deoxyribonuclease I, Recombinant, Protease and RNase Free, Animal Free/AF
Recombinant Bovine pancreatic Deoxyribonuclease 1 produced in Pichia pastoris. Chromatographically purified. Free of animal derived components, RNases, and proteases. A lyophilized powder containing glycine as a stabilizer.
Store at 2-8°C. PROTECT FROM MOISTURE.
≥5000 units per mg protein DR1 10 ku LS006361 $180.00
Add to Cart
50 ku LS006362 $755.00
Add to Cart
Bulk (ku) LS006360 Inquire

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