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Worthington Biochemical Papain

Source: Carica papaya Latex

I.U.B.: 3.4.22.2

Papain is a sulfhydryl protease from Carica papaya latex. It has a molecular weight of 23 kDa and an optimum pH range of 6.0-7.0.  The action of papain on leucine methyl ester produces an insoluble polyleucine peptide. Papain breaks down the intercellular matrix of cartilage. Papain is activated by cysteine, sulfide, and sulfite. Stabilizing agents are EDTA, cysteine and dimercaptoethanol.

Stability/Storage: Stable for 6-12 months at 2-8°C.  Do not freeze aqueous suspensions.

Technical Notes: Papain preparations should be incubated in the activation solution before use to ensure full activity. Applications include antibody fragmentation and primary/neural cell isolation.

Unit Definition: One Unit hydrolyzes one micromole of benzoyl-L-arginine ethyl ester per minute at 25°C, pH 6.2, after activation in a solution containing 1.1 mM EDTA, 0.067 mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes.

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Papain Dissociiation System (PDS/PDS2)

Description Activity Code Size Cat No. Price
PDS Kit, Papain Vial
A component of the Papain Dissociation System, for use in the tissue dissociation method of Huettner, J.E., and Baughman, R.W.: J. Neuroscience, 6, 3044 (1986). Contains papain, L-cysteine, and EDTA. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of EBSS or equivalent yields a solution at 20 units of papain per ml in 1mM L-cysteine with 0.5mM EDTA.
Store at 2-8°C.
≥100 units per vial PAP2 1 vi LK003176 $25.00
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5 vi LK003178 $85.00
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Papain, Suspension
Supplied as a two times crystalline suspension in 50mM sodium acetate, pH 4.5. To ensure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use.
Store at 2-8°C.
Activates to at least 20 units per mg protein PAP 25 mg LS003124 $34.00
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100 mg LS003126 $75.00
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1 gm LS003127 $495.00
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Bulk (mg) LS003128 Inquire
Papain, Lyophilized
Supplied as a lyophilized powder prepared from a two times crystalline suspension, Code: PAP. To ensure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use.
Store at 2-8°C.
Activates to at least 15 units per mg protein PAPL 25 mg LS003118 $37.00
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100 mg LS003119 $87.00
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1 gm LS003120 $570.00
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Bulk (mg) LS003122 Inquire

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