Collagenase Product Information

Source: Clostridium histolyticum

I.U.B.: 3.4.24.3

Collagenase Raw Material Certification 

Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca2+ is required for enzyme activity. Particular enzymatic profiles of each collagenase have been correlated with the tissues from which the cells for study were obtained (or with the uses to which the cells are put) and as a result of the correlations several types of crude collagenases have been established by Worthington: Types 1, 2, 3, and 4.

Type 1 crude collagenase has the original balance of collagenase, caseinase, clostripain and tryptic activities.
Type 2 contains higher relative levels of protease activity, particularly clostripain.
Type 3 contains lowest levels of secondary proteases.
Type 4 is designed to be especially low in tryptic activity to limit damage to membrane proteins and receptors.
• Purified collagenase, Code: CLSPA, contains minimal secondary proteolytic activities along with high collagenase activity.

NEW! Animal Origin Free Types AFA, AFB and AFC, STZ1, and STZ2 collagenases are derived from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of potential animal derived pathogens must be prevented. Levels of secondary proteases are similar to Types 1 and 2 collagenase.

• CLSAFA is the original AFA grade designed to have collagenase and secondary proteases similar to Types 1 and 2 collagenase.
• CLSAFB contains higher collagenase and caseinase activities than CLSAFA.
• CLSAFC has especially low tryptic activity similar to Type 4 collagenase.
• STZ1 & STZ2, 0.22μ filtered STEMxyme® AOF Collagenase/Neutral Protease (Dispase®) blends for primary and stem cell isolation. 

Worthington also offers 0.22µm filtered preparations of each type in pre-packaged form for direct reconstitution and use in cell isolation and culture procedures. Correlations between enzyme type and effectiveness with different tissues have been good, but not perfect, due in part to the variable parameters of use. Nevertheless most researchers consider the tissue-typing of crude collagenase lots to be a valuable service. A detailed description of the Worthington collagenase and contaminant assays can be found in the Worthington Enzyme Manual. In addition, tissue specific references and detailed isolation conditions can be found in the Worthington Tissue Dissociation Guide.

The collagenase assay is a modification of Mandl wherein collagenase is incubated for five hours with native collagen and the extent of collagen breakdown is determined using the Moore and Stein, JBC, 176, 367, (1948) colorimetric ninhydrin method. Amino acids released are expressed as micromoles leucine per milligram collagenase.

Uses: Crude collagenases are widely used in enzymatic primary cell isolation and tissue dissociation procedures. Most researchers employ either crude collagenase preparations such as Types 1, 2, 3, and 4 or chromatographically purified collagenase (Code: CLSPA); the latter usually combined with secondary enzymes such as elastase, hyaluronidase, etc. For best results, the precise mixture of proteolytic activities must be tailored to the tissue to be dissociated. Correlations between type and effectiveness with different tissues have been good, but not perfect, and may be dependent partly on parameters of use and objectives as well as lot-to-lot variations. For more information see the Collagenase Sampling Program information. Worthington also publishes a Tissue Dissociation Guide which provides additional information regarding the enzymes used for these applications and specific references for numerous cell and tissue types.

Unit Definition: One Unit releases one micromole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5

Related Products:

Collagen (CL)
Deoxyribonuclease I (DP/D/DCLS/D2/DPFF/DPRF)
Elastase (ES/ESL/ESFF)
Hyaluronidase (HSE/HSEP)
Neutral Protease (Dispase, NPRO)
Papain (PAP/PAPL/PAP2)Proteinase K (PROK)
Trypsin (TL/TRL/TRL3/TRLS/TRTPCK)
Trypsin Inhibitors (LBI/OI/SI/SIC)

Cell Isolation Optimizing System (CIT)
Hepatocyte Isolation System (HIS)
Neonatal Cardiomyocyte Isolation System (NCIS)
Papain Dissociiation System (PDS/PDS2)

DescriptionActivityCodeSizeCat. No.Price
Collagenase, Purified
Chromatographically purified. ≤ 50 caseinase units per milligram. Supplied as a lyophilized powder.
Store at 2-8C.
≥500 units per mg dry weight CLSPA 4 ku LS005275 $80.00 Buy Now
10 ku LS005273 $164.00 Buy Now
Bulk LS005277 Inquire
Collagenase Vial, NCIS
A component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of HBSS or equivalent yields a solution of 300 units/ml of collagenase, Code: CLSPA. Suitable for cell isolation and culture applications.
Store at 2-8C.
≥500 units per mg dry weight CLSPANK 1 vi LK003240 $30.00 Buy Now
5 vi LK003245 $120.00 Buy Now
Collagenase/Elastase Vial (CLSH)
Enzyme vial of HIS kit that contains 20,000 units of collagenase (Code: CLS-1) and 30 units of elastase (Code: ESL), filtered through 0.22 micron membrane and lyophilized.
Store at 2-8C.
≥20,000 units collagense/30 units elastase per vial CLSH 1 vi LK002066 $50.00 Buy Now
5 vi LK002067 $219.00 Buy Now
Collagenase, Type 1
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase, clostripain and tryptic activities. Suggested for epithelial, liver, lung and adrenal primary cell isolations. A dialyzed, lyophilized powder.
Store at 2-8C.
≥125 units per mg dry weight CLS-1 100 mg LS004194 $35.00 Buy Now
Bulk LS004193 Inquire
1 gm LS004196 $182.00 Buy Now
5 gm LS004197 $765.00 Buy Now
Bulk LS004200 Inquire
Collagenase, Type 2
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and salivary primary cell isolation. Supplied as a dialyzed, lyophilized powder.
Store at 2-8C.
≥125 units per mg dry weight CLS-2 100 mg LS004174 $35.00 Buy Now
1 gm LS004176 $182.00 Buy Now
5 gm LS004177 $765.00 Buy Now
Bulk LS004179 Inquire
Collagenase, Type 3
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. Suggested for mammary primary cell isolation. A dialyzed, lyophilized powder.
Store at 2-8C.
≥100 units per mg dry weight CLS-3 100 mg LS004180 $35.00 Buy Now
1 gm LS004182 $182.00 Buy Now
5 gm LS004183 $765.00 Buy Now
Bulk LS004185 Inquire
Collagenase, Type 4
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity. Suggested for pancreatic islet primary isolation. A dialyzed, lyophilized powder.
Store at 2-8C.
≥160 units per mg dry weight CLS-4 100 mg LS004186 $35.00 Buy Now
1 gm LS004188 $182.00 Buy Now
5 gm LS004189 $765.00 Buy Now
Bulk LS004191 Inquire
New!
Collagenase, Type 5
Partially purified to contain higher collagenase and caseinase activities. A dialyzed, lyophilized powder.
Store at 2-8C.
≥450 units per mg dry weight CLS-5 100 mg LS005280 $42.00 Buy Now
1 gm LS005282 $212.00 Buy Now
5 gm LS005283 $890.00 Buy Now
Bulk LS005284 Inquire
New!
Collagenase, Type 6
Prepared to contain above normal collagenase, FALGPA and Caseinase activities and designed to be enriched with Type II (col H) collagenase.
Store at 2-8C.
≥400 units per milligram dry weight CLS-6 100 mg LS005318 $48.00 Buy Now
500 mg LS005319 $192.00 Buy Now
2.5 gm LS005321 $525.00 Buy Now
Bulk LS005323 Inquire
New!
Collagenase, Type 7
Prepared to contain 4-5X more collagenase, FALGPA and Caseinase activities than Collagenase 1-4 and with moderate clostripain and tryptic activities.
 
 
Store at 2-8C.
≥1,000 units per milligram dry weight CLS-7 100 mg LS005332 $55.00 Buy Now
500 mg LS005333 $215.00 Buy Now
2.5 gm LS005335 $625.00 Buy Now
Bulk LS005337 Inquire
Collagenase, Type 1, Filtered
Collagenase, Type 1 (Code: CLS-1), which is filtered through a 0.22 micron membrane and lyophilized in vials.
Store at 2-8C.
≥125 units per mg dry weight CLSS-1 50 mg LS004214 $45.00 Buy Now
5x50 mg LS004216 $162.00 Buy Now
1 gm LS004217 $420.00 Buy Now
Collagenase, Type 2, Filtered
Collagenase, Type 2 (Code: CLS-2), which is filtered through a 0.22 micron membrane and lyophilized in vials.
Store at 2-8C.
≥125 units per mg dry weight CLSS-2 50 mg LS004202 $45.00 Buy Now
5x50 mg LS004204 $162.00 Buy Now
1 gm LS004205 $420.00 Buy Now
Collagenase, Type 3, Filtered
Collagenase, Type 3 (Code: CLS-3), which is filtered through a 0.22 micron membrane and lyophilized in vials to contain 50 milligrams per vial.
Store at 2-8C.
≥100 units per mg dry weight CLSS-3 50 mg LS004206 $45.00 Buy Now
5x50 mg LS004208 $162.00 Buy Now
Collagenase, Type 4, Filtered
Collagenase, Type 4 (Code: CLS-4), which is filtered through a 0.22 micron membrane and lyophilized in vials.
Store at 2-8C.
≥160 units per mg dry weight CLSS-4 50 mg LS004210 $45.00 Buy Now
5x50 mg LS004212 $162.00 Buy Now
1 gm LS004209 $420.00 Buy Now
New!
Collagenase, Type 5, Filtered
Collagenase Type 5 (Code: CLS-5), which is filtered through a 0.22 micron membrane and lyophilized in vials.
Store at 2-8C.
≥450 units per mg dry weight CLSS-5 50 mg LS005286 $54.00 Buy Now
5x50 mg LS005287 $190.00 Buy Now
1 gm LS005288 $485.00 Buy Now
BSE-Free Collagenase (Animal Origin Free)-A
Prepared cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens must be prevented.
Store at 2-8C.
≥150 units per mg dry weight CLSAFA 100 mg LS004152 $45.00 Buy Now
1 gm LS004154 $220.00 Buy Now
5 gm LS004156 $950.00 Buy Now
Bulk LS004158 Inquire
New!
BSE-Free
Collagenase, CLSAFA, Filtered
Collagenase, Animal Origin-Free (Code: CLSAFA) which is filtered through a 0.22 micron membrane and lyophilized in vials.
Store at 2-8C.
≥150 units per mg dry weight CLSAFAS 50 mg LS004118 $58.00 Buy Now
5X50 mg LS004119 $225.00 Buy Now
New!
BSE-Free
Collagenase (Animal Origin Free) - B
Prepared from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens must be prevented.
Store at 2-8C.
≥300 units per mg dry weight CLSAFB 100 mg LS004145 $45.00 Buy Now
1 gm LS004147 $220.00 Buy Now
5 gm LS004148 $950.00 Buy Now
Bulk LS004150 Inquire
New!
BSE-Free
Collagenase, CLSAFB, Filtered
Collagenase, Animal Origin Free (Code: CLSAFB) which is filtered through a 0.22 micron membrane and lyophilized in vials.
Store at 2-8C.
≥300 units per mg dry weight CLSAFBS 50 mg LS004124 $58.00 Buy Now
5x50 mg LS004125 $225.00 Buy Now
New!
BSE-Free
Collagenase (Animal Origin Free) - C
Prepared from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens must be prevented.
Store at 2-8C.
≥200 units per mg dry weight CLSAFC 100 mg LS004138 $45.00 Buy Now
1 gm LS004140 $220.00 Buy Now
5 gm LS004141 $950.00 Buy Now
Bulk LS004143 Inquire
New!
BSE-Free
Collagenase, CLSAFC, Filtered
Collagenase Animal Origin Free (Code: CLSAFC) which is filtered through a 0.22 micron membrane and lyophilized in vials.
Store at 2-8C.
≥200 units per mg dry weight CLSAFCS 50 mg LS004130 $58.00 Buy Now
5x50 mg LS004131 $225.00 Buy Now
New!
BSE-Free
STEMxyme 1, Collagenase/Neutral Protease (Dispase)
A specialized combination of Animal Origin-Free Clostridium histolyticum collagenase (Code: CLSAFB) and animal origin free Bacillus polymyxa neutral protease with a minimum of 250 CLS units and 1,000 caseinase units per mg dry weight. Designed for stem cell and other primary cell isolations and bioprocessing applications where introduction of potential animal derived pathogens must be prevented.
Store at 2-8C.
≥250 CLS/1,000 CAS units per milligram dry weight STZ1 50 mg LS004106 $86.00 Buy Now
5x50 mg LS004107 $395.00 Buy Now
New!
BSE-Free
STEMxyme 2 Collagenase/Neutral Protease (Dispase)
A specialized combination of Animal Origin-Free Clostridium histolyticum collagenase (Code: CLSAFB) and animal origin free Bacillus polymyxa neutral protease with a minimum of 250 CLS units and 2,000 caseinase units per mg dry weight. Designed for stem cell and other primary cell isolations and bioprocessing applications where introduction of potential animal derived pathogens must be prevented.
Store at 2-8C.
≥250 CLS/2,000 CAS units per milligram dry weight STZ2 50 mg LS004112 $132.00 Buy Now
5x50 mg LS004113 $585.00 Buy Now

Up: Worthington Product Catalog