Description
DNA Polymerase, _Taq_
Source:
N/A
Code
DNAPTAQ
DNA Polymerase, Taq - Assay
CAS:
9012-90-2
DNA Polymerase, <i>Taq</i> Assay
Method
One unit incorporates 10 nanomoles of total deoxynucleotides into acid precipitable products with activated calf thymus DNA as primer-template, for 30 min. at 37°C, prepared according to Aposhian and Kornberg 1962.
Reagents
150 mM Tris•HCl, pH 8.5 with 5 mM ammonium sulfate, 24 mM MgCl2, 30 mM 2-mercaptoethanol and 0.2 mg/ml BSA
Nucleoside Triphosphates: 30 μM dATP, dCTP, dGTP, dTTP spiked with [3H]dTTP to 106 - 2 X 106.
0.5 mg/ml activated calf thymus DNA
10% perchloric acid
1% perchloric acid
Methyl Cellosolve® (ethylene glycol monomethyl ether)
Calculation
DNA Polymerase, Taq Products
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