Trypsinogen - Manual

Source:
Bovine Pancreas
CAS:
9002-08-8

Trypsinogen is the 24kDa inactive precursor of trypsin which nay be activated by enzymatic removal of an n-terminal hexapeptide to yield single chain beta-trypsin. Subsequent limited autolysis produces other active forms having two or more peptide chains bound by disulfide bonds. The predominent forms are alpha-trypsin, having two peptide chains and beta-trypsin, a single chain.

Composition

Trypsinogen has been shown to have certain intrinsic activity. See Knights and Light (1974) for further references. It is usually considered, however, as the inactive precursor of trypsin which may be activated by removal of a terminal hexapeptide to yield single-chain β-trypsin. Subsequent limited autolysis produces other active forms having two or more peptide chains bound by disulfide bonds. The predominant forms are α-trypsin, having two peptide chains and β-, a single chain. Different activity and thermal stability are shown by α- and β-trypsin.

Characteristics of Trypsinogen
Molecular Weight

Trypsinogen: 24,000 (Walsh and Neurath 1964).

Isoelectric point

Trypsinogen: pH 9.3 (Walsh and Neurath 1964).

Activators

The rate of trypsinogen conversion is enhanced by using lanthanide in place of calcium ions (Gomez et al. 1974).

Stabilizers

Calcium ion retards trypsin autolysis and promotes activation of trypsinogen. Sipos and Merkel (1970) have reported on the calcium-trypsin complex. See also Griffiths and Brechner (1973).

Trypsinogen Products

Description
Activity
Code
Cat. #
Size
Price
Description
Trypsinogen
Source:
Bovine Pancreas
A 1X crystallized lyophilized powder. Intrinsic trypsin activity 0.5 units/mg of TAME activity.
Store at 2-8°C. PROTECT FROM MOISTURE.
Activity
Activates to at least 150 units per mg dry weight
Code
TG
Minimum order quantity
20000
LS003652
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 20000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
LS003649
1 gm
$54.00
LS003648
500 mg
$31.00